BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0169.p (324 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310893|ref|YP_003774985.1| dipeptide/oligopeptide/nickel A... 603 e-174 gi|300312480|ref|YP_003776572.1| dipeptide/oligopeptide/nickel A... 207 1e-54 gi|300311170|ref|YP_003775262.1| dipeptide/oligopeptide/nickel A... 185 5e-48 gi|300310464|ref|YP_003774556.1| dipeptide/oligopeptide/nickel A... 125 4e-30 gi|300312718|ref|YP_003776810.1| ABC transporter permease [Herba... 119 3e-28 >gi|300310893|ref|YP_003774985.1| dipeptide/oligopeptide/nickel ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 324 Score = 603 bits (1555), Expect = e-174, Method: Compositional matrix adjust. Identities = 316/323 (97%), Positives = 321/323 (99%) Query: 1 MSGAIAKSVLQALMVVLLMTVIVFFGLHMIGNPVDILIGQDVDQVDRLRIIKELGLDQPV 60 MSGAIA+SV+QALMVVLLMTVIVFFGLHMIGNPVDILIGQDVDQVDRLRII+ELGLDQPV Sbjct: 1 MSGAIARSVVQALMVVLLMTVIVFFGLHMIGNPVDILIGQDVDQVDRLRIIQELGLDQPV 60 Query: 61 WRQYLAFLNGALHGNLGNSFVYNTPAILLIFQRLPATLELAVAALLLALAVGVPLGLFAG 120 WRQYLAFLNGALHGNLGNSFVYNTPAILLIFQRLPATLELA AALLLAL VGVPLGLFAG Sbjct: 61 WRQYLAFLNGALHGNLGNSFVYNTPAILLIFQRLPATLELACAALLLALVVGVPLGLFAG 120 Query: 121 MKPDHPFSRLVMTTSIFGFSLPTFWIGLMLIMAFSVTLGWLPSGGRGQTAELFGVQWSWL 180 MKPDHPFSRLVMTTSIFGFSLPTFWIGLMLIMAFSVTLGWLPSGGRGQTAELFGVQWSWL Sbjct: 121 MKPDHPFSRLVMTTSIFGFSLPTFWIGLMLIMAFSVTLGWLPSGGRGQTAELFGVQWSWL 180 Query: 181 TADGWRHMILPAINLSLFKISLVIRLTRANVREVLPMDYVKFARAKGLSPARVVLMYVLR 240 TADGWRHMILPAINLSLFKISLVIRLTRANVREVLPMD+VKFARAKGLSPARVVLMYVLR Sbjct: 181 TADGWRHMILPAINLSLFKISLVIRLTRANVREVLPMDFVKFARAKGLSPARVVLMYVLR 240 Query: 241 NTLIPLVTVVGLEFGSTIAFAVVTESIFAWPGAGKLILDSINALDRPVIVAYLMVIVCMF 300 NTLIPLVTVVGLEFGSTIAFAVVTESIFAWPGAGKLILDSINALDRPVIVAYLMVIVCMF Sbjct: 241 NTLIPLVTVVGLEFGSTIAFAVVTESIFAWPGAGKLILDSINALDRPVIVAYLMVIVCMF 300 Query: 301 VTINLVVDVLYKLLDPRVRVEAR 323 VTINLVVDVLYKLLDPRVRVEA+ Sbjct: 301 VTINLVVDVLYKLLDPRVRVEAK 323 >gi|300312480|ref|YP_003776572.1| dipeptide/oligopeptide/nickel ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 305 Score = 207 bits (526), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 115/312 (36%), Positives = 183/312 (58%), Gaps = 20/312 (6%) Query: 12 ALMVVLLMTVIVFFGLHMIGNPVDILIGQDVD--QVDRLRIIKELGLDQPVWRQYLAFLN 69 AL V + ++V+ F LHM G+ + G + QV+ +R + GLD+P+ QYL +L Sbjct: 12 ALCVAVTVSVVSFTLLHMSGDLAVSIAGPEATAAQVESVR--TQFGLDRPMATQYLDWLG 69 Query: 70 GALHGNLGNSFVYNTPAILLIFQRLPATLELAVAALLLALAVGVPLGLFAGMKPDHPFSR 129 A+ + G SF + P + +I +RLP T +L +ALLLA+ +PLG+ A + D R Sbjct: 70 RAVQLDFGKSFYFQGPVMEMIAERLPITFKLGGSALLLAVLTAIPLGVLAAIYRDTWLDR 129 Query: 130 LVMTTSIFGFSLPTFWIGLMLIMAFSVTLGWLPSGGRGQTAELFGVQWSWLTADGWRHMI 189 + ++ G ++P+FW GL LI+ F+VTL WLP G + W+H I Sbjct: 130 AALLIAVVGQAMPSFWFGLTLILIFAVTLHWLPVAGN----------------ESWQHFI 173 Query: 190 LPAINLSLFKISLVIRLTRANVREVLPMDYVKFARAKGLSPARVVLMYVLRNTLIPLVTV 249 LPA+ L + + ++RLTR+ + EVL DY++ ARAKGLS +RVV + LRN +IP+V + Sbjct: 174 LPAVALGYYAMPAMMRLTRSGMLEVLGSDYIRTARAKGLSKSRVVFKHALRNAVIPVVAL 233 Query: 250 VGLEFGSTIAFAVVTESIFAWPGAGKLILDSINALDRPVIVAYLMVIVCMFVTINLVVDV 309 +E G + +VV ES+++ G G+L D+I+ D PV+ A +++I ++ + + DV Sbjct: 234 AAVELGFMLGGSVVIESVYSMQGLGQLAWDAISRNDYPVVQAVVLIIAMFYIGLTFLADV 293 Query: 310 LYKLLDPRVRVE 321 + LLDPR+R Sbjct: 294 INALLDPRIRTR 305 >gi|300311170|ref|YP_003775262.1| dipeptide/oligopeptide/nickel ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 306 Score = 185 bits (469), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 105/291 (36%), Positives = 167/291 (57%), Gaps = 16/291 (5%) Query: 31 GNPVDILIGQDVDQVDRLRIIKELGLDQPVWRQYLAFLNGALHGNLGNSFVYNTPAILLI 90 G+P + G + D + +LGLD+P+ Q++ F A G+ G+S P I Sbjct: 32 GDPARLAAGPEADDKTVALVRADLGLDKPLPEQFVNFFLNAARGDFGHSIRTKRPVSEEI 91 Query: 91 FQRLPATLELAVAALLLALAVGVPLGLFAGMKPDHPFSRLVMTTSIFGFSLPTFWIGLML 150 R TL L V A++ A+ G+ +G+ + + + RL MT ++ G S P F +G++L Sbjct: 92 SARFWPTLWLTVVAMVWAVLFGLIIGISSAVWRNQWPDRLGMTLAVSGISFPAFSLGILL 151 Query: 151 IMAFSVTLGWLPSGGRGQTAELFGVQWSWLTADGWRHMILPAINLSLFKISLVIRLTRAN 210 + FSV LGWLP+ G D W H ILP+I L +++ R TR++ Sbjct: 152 VQVFSVKLGWLPTVGD----------------DTWLHYILPSITLGAAVAAVMARFTRSS 195 Query: 211 VREVLPMDYVKFARAKGLSPARVVLMYVLRNTLIPLVTVVGLEFGSTIAFAVVTESIFAW 270 E+L D+V+ ARAKGL+ VV+ + LRN LIP+VT++GL+FG + +++ E +F W Sbjct: 196 FIEILQEDFVRTARAKGLTETVVVIKHCLRNALIPVVTMMGLQFGFLLGGSILVEVVFNW 255 Query: 271 PGAGKLILDSINALDRPVIVAYLMVIVCMFVTINLVVDVLYKLLDPRVRVE 321 PG G+L++D++ D PVI A +++ F+ INLVVDVLY +++P +R + Sbjct: 256 PGMGRLLVDAVEMRDYPVIQAEILLFSLEFIFINLVVDVLYAVINPSIRFK 306 >gi|300310464|ref|YP_003774556.1| dipeptide/oligopeptide/nickel ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 318 Score = 125 bits (314), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 89/302 (29%), Positives = 151/302 (50%), Gaps = 13/302 (4%) Query: 23 VFFGLHMI-GNPVDILIGQDV--DQVDRLRIIKELGLDQPVWRQYLAFLNGALHGNLGNS 79 VFF +++ G+ +GQ + V LR+ + GLD P +Y +L L GN G S Sbjct: 27 VFFITNLLPGDAAQTALGQAATPETVAALRL--QYGLDLPAPLRYAHWLGNLLSGNPGMS 84 Query: 80 FVYNTPAILLIFQRLPATLELAVAALLLALAVGVPLGLFAGMKPDHPFSRLVMTTSIFGF 139 V N P +I RLPA+L LA L+++ + + LG+F+ M F R V +++ Sbjct: 85 LVNNVPVAQMIGGRLPASLMLAAVTALVSVPLALFLGIFSAMYRGSFFDRAVNVSAVSMV 144 Query: 140 SLPTFWIGLMLIMAFSVTLGWLPSGGRGQTAELFGVQWSWLTADGWRHMILPAINLSLFK 199 S+P F + + +M F+V L WL + A G +++ A +P + L Sbjct: 145 SVPEFLVATVAVMIFAVKLRWLSALSHAGDAATLG---AFIKAYA-----MPVLTLCCVI 196 Query: 200 ISLVIRLTRANVREVLPMDYVKFARAKGLSPARVVLMYVLRNTLIPLVTVVGLEFGSTIA 259 ++ + R+TRA V + L Y + A KG+ R+VL + L N + P+ V L + Sbjct: 197 VAQMTRMTRAAVIDQLRSSYAEMALLKGVKRTRIVLRHALPNAIGPIANAVALSLSYLLG 256 Query: 260 FAVVTESIFAWPGAGKLILDSINALDRPVIVAYLMVIVCMFVTINLVVDVLYKLLDPRVR 319 ++ ESIF +PG L++D++ D P++ A M+ ++ + L DVL + +PR++ Sbjct: 257 GVIIVESIFNYPGIATLMIDAVTTRDMPLVQACSMIFCAGYLLLVLTADVLAIVSNPRLK 316 Query: 320 VE 321 Sbjct: 317 TR 318 >gi|300312718|ref|YP_003776810.1| ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 356 Score = 119 bits (299), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 91/294 (30%), Positives = 148/294 (50%), Gaps = 16/294 (5%) Query: 40 QDVDQVDRLRIIKEL-GLDQPVWRQYLAFLNGALHGNLGNSFVYNTPAILLIFQRLPATL 98 Q +D+ R+ IK+L G D+P ++ L G L +LG SF ++ L+ +LP ++ Sbjct: 69 QGIDE-QRIAEIKQLYGFDKPPVERFWTMLKGFLTFDLGKSFYHHGDVWTLVKSKLPVSI 127 Query: 99 ELAVAALLLALAVGVPLGLFAGMKPDHPFSRLVMTTSIFGFSLPTFWIGLMLIMAFS--V 156 + + LL+ + VPLG+ ++ F + G+S+P F +G+ L++ F+ Sbjct: 128 SIGLWTFLLSYLISVPLGIAKAVREGTRFDTATTGLVLVGYSIPGFVLGVFLLVLFAGGS 187 Query: 157 TLGWLPSGGRGQTAELFGVQWSWLT-----ADGWRHMILPAINLSLFKISLVIRLTRANV 211 + W P RG T++ W L+ D H+ LP + +++ LTR Sbjct: 188 FVQWFPL--RGLTSD----NWESLSLMGKITDYLWHITLPVTASVIGSFAVITMLTRNTF 241 Query: 212 REVLPMDYVKFARAKGLSPARVVLMYVLRNTLIPLVTVVGLEF-GSTIAFAVVTESIFAW 270 E + YV ARAKGLS RV+ +V RN LIPLVT F S A +++ E++F+ Sbjct: 242 LEEIRKQYVLTARAKGLSERRVLYKHVFRNALIPLVTGFPSAFIASFFAGSLLIETLFSL 301 Query: 271 PGAGKLILDSINALDRPVIVAYLMVIVCMFVTINLVVDVLYKLLDPRVRVEARA 324 G G L +S D PV++ L + + + + LV D+ Y DPRV+ E+ A Sbjct: 302 DGMGLLAYESAIRRDYPVVMGTLYLFTLIGLVVKLVGDLCYVWADPRVQFESLA 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.144 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 922,748 Number of extensions: 36241 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 6 Length of query: 324 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 239 Effective length of database: 1,217,395 Effective search space: 290957405 Effective search space used: 290957405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)