BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0164.p (401 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspir... 741 0.0 gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herb... 470 e-134 gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspir... 469 e-133 gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein ... 231 1e-61 gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspir... 90 3e-19 >gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 741 bits (1912), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/392 (92%), Positives = 374/392 (95%) Query: 1 MNAPAPCRPPADLRFPIYLDYGATTPVDPHVARVMCDFLTEKFGNAASSSHPFGWEARRA 60 MNAPAPCRPPADLRFPIYLDYGATTPVDP VARVMCDFLT+KFGNAASSSHPFGWEARRA Sbjct: 1 MNAPAPCRPPADLRFPIYLDYGATTPVDPQVARVMCDFLTDKFGNAASSSHPFGWEARRA 60 Query: 61 VDKARAQVAGLVGARPDEIIWTSGATESNNLALKGCAHALRAQGRGKHLITVATEHKAVL 120 V++ARAQVAGLVGA+P EI+WTSGATESNNLALKG A+ALRAQGRG HLITV TEHKAVL Sbjct: 61 VEQARAQVAGLVGAQPGEIVWTSGATESNNLALKGAAYALRAQGRGNHLITVPTEHKAVL 120 Query: 121 DTMHTLEKSGFEVTYLQPGADGLITPAQFRAALRPDTLLASVMYVNNEIGVIQPIAELGE 180 DTMHTLEK+GFEVTYLQPGADGLITP Q RAALRPDTLLASVMYVNNEIGVIQPIAELGE Sbjct: 121 DTMHTLEKAGFEVTYLQPGADGLITPQQLRAALRPDTLLASVMYVNNEIGVIQPIAELGE 180 Query: 181 LCAERGVVFHVDAVQAAGKIAIDLQQLKVDLMSFSAHKVYGPKGIGALYVRHNPALKLEA 240 +CA VVFHVDAVQAAGKIAIDL +LKVDLMSFSAHKVYGPKGIGALYVRHNP LKLEA Sbjct: 181 ICAAHDVVFHVDAVQAAGKIAIDLTRLKVDLMSFSAHKVYGPKGIGALYVRHNPQLKLEA 240 Query: 241 QIDGGGHENGMRSGTLATHQIVGMGEAFALAGDLLEEEGRRIRALRDRLWAGLADLPGVQ 300 QIDGGGHENGMRSGTLATHQIVGMGEAFALAG LLEEE RRI ALRDRLWAGLADLPGVQ Sbjct: 241 QIDGGGHENGMRSGTLATHQIVGMGEAFALAGRLLEEESRRIGALRDRLWAGLADLPGVQ 300 Query: 301 LNGSAEARVPHNLNVSFPPTRYGTLGSQLLGIAVSAGSACNSAASAPSFVLLAIGRSPDV 360 LNG A RVPHNLNVSFPPTR+GTLGSQLLGIAVSAGSACNSAA+APSFVLLAIGRSPDV Sbjct: 301 LNGCARQRVPHNLNVSFPPTRHGTLGSQLLGIAVSAGSACNSAAAAPSFVLLAIGRSPDV 360 Query: 361 ARNAVRFSLGRGTTEAEIDYVVATVRQVLAHE 392 ARNAVRFSLGRGTTEAEIDYVVATVRQVLAHE Sbjct: 361 ARNAVRFSLGRGTTEAEIDYVVATVRQVLAHE 392 >gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herbaspirillum seropedicae SmR1] Length = 406 Score = 470 bits (1210), Expect = e-134, Method: Compositional matrix adjust. Identities = 241/377 (63%), Positives = 283/377 (75%), Gaps = 2/377 (0%) Query: 11 ADLRFPIYLDYGATTPVDPHVARVMCDFLTEKFGNAASSSHPFGWEARRAVDKARAQVAG 70 + L PIYLDY ATTPVDP VA+ M +LT+ GN AS SH FGW A AV+ AR QVA Sbjct: 3 SSLSLPIYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVAA 62 Query: 71 LVGARPDEIIWTSGATESNNLALKGCAHALRAQGRGKHLITVATEHKAVLDTMHTLEKSG 130 LV A EIIWTSGATESNNLA+KG A LR++GR HLITV TEHKAVLDTM LE+ G Sbjct: 63 LVNADVREIIWTSGATESNNLAIKGAAEFLRSKGR--HLITVKTEHKAVLDTMRELERRG 120 Query: 131 FEVTYLQPGADGLITPAQFRAALRPDTLLASVMYVNNEIGVIQPIAELGELCAERGVVFH 190 FEVTYL+ G++GL+ A F AALRPDT+LASVMYVNNEIGVIQ IA LG++C RGV+ H Sbjct: 121 FEVTYLEVGSNGLLDLAAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLH 180 Query: 191 VDAVQAAGKIAIDLQQLKVDLMSFSAHKVYGPKGIGALYVRHNPALKLEAQIDGGGHENG 250 VDA QA GK+AIDL +LKVDLMSFSAHK YGPKGIGALYV P ++LEAQ+ GGGHE G Sbjct: 181 VDAAQATGKVAIDLARLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGHERG 240 Query: 251 MRSGTLATHQIVGMGEAFALAGDLLEEEGRRIRALRDRLWAGLADLPGVQLNGSAEARVP 310 +RSGTLATHQIVGMGEAF LA + E RIR LRDRL AG AD+ +NG E RVP Sbjct: 241 LRSGTLATHQIVGMGEAFELARLAMGTENERIRMLRDRLHAGFADIEECFVNGDLERRVP 300 Query: 311 HNLNVSFPPTRYGTLGSQLLGIAVSAGSACNSAASAPSFVLLAIGRSPDVARNAVRFSLG 370 HNLNVSF +L + +AVS+GSAC SA+ PS+VL A+G S ++A +++RFS+G Sbjct: 301 HNLNVSFNYVEGESLLMAIKDVAVSSGSACTSASLEPSYVLRALGMSDELAHSSIRFSIG 360 Query: 371 RGTTEAEIDYVVATVRQ 387 R TT EID+ V +R+ Sbjct: 361 RFTTVEEIDFTVNVIRE 377 >gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 469 bits (1207), Expect = e-133, Method: Compositional matrix adjust. Identities = 231/389 (59%), Positives = 287/389 (73%), Gaps = 5/389 (1%) Query: 1 MNAPAPCRPPADLR---FPIYLDYGATTPVDPHVARVMCDFLTEKFGNAASSSHPFGWEA 57 MNAP ++ FPIY+DY ATTPVDP VA M +L E+FGN AS SH +GW A Sbjct: 1 MNAPLEKSLLETIKAPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHMYGWTA 60 Query: 58 RRAVDKARAQVAGLVGARPDEIIWTSGATESNNLALKGCAHALRAQGRGKHLITVATEHK 117 +AV++AR +VA LV A EIIWTSGATESNNLA+KG A ++ +GKH+ITV TEHK Sbjct: 61 EKAVEEAREEVAKLVNADSREIIWTSGATESNNLAIKGAAQFYKS--KGKHIITVKTEHK 118 Query: 118 AVLDTMHTLEKSGFEVTYLQPGADGLITPAQFRAALRPDTLLASVMYVNNEIGVIQPIAE 177 AVLDT LE+ GFE TYLQP +GL+T Q AA+RPDT+L SVM VNNEIGVIQP+ E Sbjct: 119 AVLDTFRELERIGFEATYLQPQDNGLVTVEQIAAAVRPDTILVSVMLVNNEIGVIQPVPE 178 Query: 178 LGELCAERGVVFHVDAVQAAGKIAIDLQQLKVDLMSFSAHKVYGPKGIGALYVRHNPALK 237 +GE C +G++FH DA QA GK+ IDL+ KVDLMSFSAHK YGPKGIGALYVR P ++ Sbjct: 179 IGEFCRSKGIIFHSDAAQATGKVKIDLENWKVDLMSFSAHKSYGPKGIGALYVRRKPRVR 238 Query: 238 LEAQIDGGGHENGMRSGTLATHQIVGMGEAFALAGDLLEEEGRRIRALRDRLWAGLADLP 297 +EAQ+ GGGHE G+RSGTLATHQIVGMGEAF LA + ++ E RIRALRDRL GL ++ Sbjct: 239 IEAQMHGGGHERGLRSGTLATHQIVGMGEAFRLAREEMDSELARIRALRDRLAKGLQEIE 298 Query: 298 GVQLNGSAEARVPHNLNVSFPPTRYGTLGSQLLGIAVSAGSACNSAASAPSFVLLAIGRS 357 +NG E RVPHNLNVSF +L + IAVS+GSAC SA+ PS+VL A+GRS Sbjct: 299 ETYINGDMEHRVPHNLNVSFNYVEGESLIMAIKDIAVSSGSACTSASLEPSYVLRALGRS 358 Query: 358 PDVARNAVRFSLGRGTTEAEIDYVVATVR 386 ++A +++RF++GR TTE +ID+ +A ++ Sbjct: 359 DELAHSSIRFTIGRFTTEEDIDFTIALIK 387 >gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein [Herbaspirillum seropedicae SmR1] Length = 379 Score = 231 bits (588), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 159/375 (42%), Positives = 224/375 (59%), Gaps = 10/375 (2%) Query: 17 IYLDYGATTPVDPHVARVMCDFLTEKFGNAASSSHPFGWEARRAVDKARAQVAGLVGARP 76 IYLD ATT P V M E +GN SS H G AR+ ++ ARA+VA L+ A Sbjct: 5 IYLDNNATTRPTPEVLAAMQACQDEAWGNP-SSKHRVGDAARQRMNSARAEVAALLAASA 63 Query: 77 DEIIWTSGATESNNLALKGCAHALRAQGRGKHLITVATEHKAVLDTMHTLEKSGFEVTYL 136 E+++TSGATE+N++A+ G AL Q + +H+++ EH + L + L+ G ++++ Sbjct: 64 AELVFTSGATEANHMAILG---ALALQPQRRHIVSSLVEHPSTLLLLRHLQAQGVRISWI 120 Query: 137 QPGADGLITPAQFRAALRPDTLLASVMYVNNEIGVIQPIAELGELCAERGVVFHVDAVQA 196 G + + AA+ PDT L S+M+ NNE GV+ PIA+ EL +RGV+FH DAVQA Sbjct: 121 AVDEHGQLQLDELAAAITPDTALVSLMWANNETGVVFPIAQAAELARQRGVLFHTDAVQA 180 Query: 197 AGKIAIDLQQLKVDLMSFSAHKVYGPKGIGALYVRHNPALKLEAQIDGGGHENGMRSGTL 256 G++ D+ L VDL+S SAHK++GPKGIGALYVR +KL + G E G R GT Sbjct: 181 VGRVPCDVAGLGVDLLSLSAHKLHGPKGIGALYVRKG--IKLPPLL-FGHQERGRRGGTE 237 Query: 257 ATHQIVGMGEAFALAGDLLEEEGRRIRALRDRLWAGLADLPGVQLNGSAEARVPHNLNVS 316 IVG+G A A A L + + ALR R GLA LP V++NG+ AR+P+ ++V Sbjct: 238 DVAGIVGLGVAAAQARAALPRM-QAVSALRQRFERGLARLPQVKINGAGAARLPNTVSVR 296 Query: 317 FPPTRYGTLGSQL--LGIAVSAGSACNSAASAPSFVLLAIGRSPDVARNAVRFSLGRGTT 374 F + +L LGI S+GSAC ++ PS VL+A+G S + A +RFSL R T Sbjct: 297 FGQVHAELVLERLDRLGICASSGSACTASGDMPSHVLMAMGMSSEQAVATIRFSLSRDTR 356 Query: 375 EAEIDYVVATVRQVL 389 E EID V+A + Q++ Sbjct: 357 EDEIDAVLAALLQIV 371 >gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspirillum seropedicae SmR1] Length = 632 Score = 90.1 bits (222), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 112/401 (27%), Positives = 165/401 (41%), Gaps = 47/401 (11%) Query: 17 IYLDYGATTPVDPHVARVMCDFLTEKFGNAASSSHPFGWEARRAVDKARAQVAGLVGARP 76 I+ D ATT V + +F + N ++H A A + AR +V + A P Sbjct: 249 IWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERVRAFLNA-P 307 Query: 77 D--EIIWTSGATESNNLALK--GCAHALRAQGRGKHLITVATEHKAVLDTMHTLE-KSGF 131 D E+I+ G TE+ NL K G H G G +I EH A + L + G Sbjct: 308 DVNEVIFVRGTTEAINLVAKSWGGKHV----GAGDEIIVSHLEHHANIVPWQQLAAEKGA 363 Query: 132 EVTYLQPGADGLITPAQFRAALRPDTLLASVMYVNNEIGVIQPIAELGELCAERGVVFHV 191 ++ + G + ++ L T + +V V+N +G I P ++ EL G V Sbjct: 364 KLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGARTLV 423 Query: 192 DAVQAAGKIAIDLQQLKVDLMSFSAHKVYGPKGIGALYVRHNPALKLEAQIDGGGH---- 247 D Q+ + D+Q L D FS HKV+GP GIGAL+ R + L+ GGG+ Sbjct: 424 DGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRD-VLEDMPPWQGGGNMIAD 482 Query: 248 -----------ENGMRSGTLATHQIVGMGEAFALAGDL-LEEEGRRIRALRDRLWAGLAD 295 N +GT VG+G A + ++ AL + L + Sbjct: 483 VTFEKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRVGIDNIAAYEHALLEYGMHQLGE 542 Query: 296 LPGVQLNGSAEARVPHNLNV-SFPPTRYGT--LGSQL--LGIAVSAGSACNSAASAPSFV 350 + G++L G+A H +V SF Y T +G L GIAV G C + Sbjct: 543 IEGLRLIGTAA----HKASVMSFVLEGYSTEDVGKALNQEGIAVRTGHHCAQP------I 592 Query: 351 LLAIGRSPDVARNAVRFSLGRGTTEAEIDYVVATVRQVLAH 391 L G VR SL T EID ++ VR++ A Sbjct: 593 LRRFG-----VETTVRPSLAFYNTFEEIDCLLTVVRRLAAQ 628 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,305,531 Number of extensions: 56261 Number of successful extensions: 215 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 5 Length of query: 401 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 314 Effective length of database: 1,207,925 Effective search space: 379288450 Effective search space used: 379288450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)