BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0161.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310887|ref|YP_003774979.1| metal ion ABC transporter perip... 504 e-144 gi|300310325|ref|YP_003774417.1| metal ion ABC transporter perip... 214 5e-57 gi|300314030|ref|YP_003778122.1| metal ion ABC transporter perip... 192 2e-50 gi|300311120|ref|YP_003775212.1| metal ion ABC transporter perip... 183 1e-47 gi|300311637|ref|YP_003775729.1| metal ion ABC transporter perip... 115 5e-27 >gi|300310887|ref|YP_003774979.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 504 bits (1298), Expect = e-144, Method: Compositional matrix adjust. Identities = 250/263 (95%), Positives = 261/263 (99%) Query: 1 MIRKLIPHLVAGAALAFSVGAHAADKLVIAATPVPHAEILEHIKPALAKEGIELQVKVFT 60 MIRKLIPHL AGAALAFSVGAHAADKLVIAATPVPHAEILEHIKPALAKEGI+LQVKVFT Sbjct: 1 MIRKLIPHLFAGAALAFSVGAHAADKLVIAATPVPHAEILEHIKPALAKEGIDLQVKVFT 60 Query: 61 DYIQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKFKKVAD 120 DY+QPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQK+KK+AD Sbjct: 61 DYVQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKYKKIAD 120 Query: 121 IPDGATIAIPNDPSNSGRALLLLARNGLIKLKDSSNISSTQKDIVENPKKLKFRALEAAT 180 IP+GATIAIPNDPSNSGRALLLLARNGLIKLKD++NIS+TQKDIVENPKKLKFRALEAAT Sbjct: 121 IPNGATIAIPNDPSNSGRALLLLARNGLIKLKDNTNISATQKDIVENPKKLKFRALEAAT 180 Query: 181 LPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSAAIKKLAA 240 LPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDS AIKKLAA Sbjct: 181 LPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSPAIKKLAA 240 Query: 241 ALNTPDVKKFIEEKYKGAVVPAF 263 ALN+PDVKKFIEEKY+GA+VPAF Sbjct: 241 ALNSPDVKKFIEEKYQGAIVPAF 263 >gi|300310325|ref|YP_003774417.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 265 Score = 214 bits (546), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 116/266 (43%), Positives = 163/266 (61%), Gaps = 4/266 (1%) Query: 1 MIRKLIPHLVAGAALA---FSVGAHAADKLVIAATPVPHAEILEHIKPALAKEGIELQVK 57 M R+ + +AG LA S A A D++ + T PHAEI+E +K L K+G++++V Sbjct: 1 MKRRQLIQFIAGLGLAAGLVSAPAFAQDQIKMGVTAGPHAEIMEQVKKLLEKDGVQMKVI 60 Query: 58 VFTDYIQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKFKK 117 FTDYIQP A +D N + HQPYLD K V V P YS+K K Sbjct: 61 EFTDYIQPNAALAAGDLDANSYQHQPYLDAQIKDRGYKF-VSVGSTITFPMGVYSKKIKS 119 Query: 118 VADIPDGATIAIPNDPSNSGRALLLLARNGLIKLKDSSNISSTQKDIVENPKKLKFRALE 177 + D+ GA + +PNDP+N GRALL+L G+IKLK + + +T DIVENPKK+K L+ Sbjct: 120 LNDLKQGARVGVPNDPTNGGRALLVLQAKGVIKLKADAGLKATPLDIVENPKKIKIVELD 179 Query: 178 AATLPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSAAIKK 237 AA LPR L+ D A+IN NYA A L+P KD++ +E + PYAN++ R +KD + K Sbjct: 180 AAQLPRSLDDFDAAVINGNYAESAGLSPTKDAIAVEASTGPYANVIAVRIADKDKPWVAK 239 Query: 238 LAAALNTPDVKKFIEEKYKGAVVPAF 263 L A ++P+VKKF+ EKYKG+V+ ++ Sbjct: 240 LVKAYHSPEVKKFVLEKYKGSVITSW 265 >gi|300314030|ref|YP_003778122.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 276 Score = 192 bits (488), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 101/270 (37%), Positives = 161/270 (59%), Gaps = 8/270 (2%) Query: 1 MIRKLIPHLVAG-AALAFSVGAHAADKLVIAATPVPHAEILEHIKPALAKEGIELQVKV- 58 +R+L +V+ A+L + A A + + T PHA+I E + ++ +VK+ Sbjct: 8 WLRRLSTLMVSMLASLVLNAQAAADQVIRVGVTSGPHAQIFEQARRVFERDNPGYKVKII 67 Query: 59 -FTDYIQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKFKK 117 F DYIQP A + ++D N + H+P+L+ K+ + K + P A YS+K++K Sbjct: 68 EFNDYIQPNAALDAGELDANSYQHRPFLNAQIKTRGYKLYAE-GKTMIGPMAIYSRKYRK 126 Query: 118 VADIPDGATIAIPNDPSNSGRALLLLARNGLIKLKDS----SNISSTQKDIVENPKKLKF 173 + D+P GA I IPNDP+N R LLLL ++G IKL+ + ++T DI ENPKK KF Sbjct: 127 LEDVPVGARIGIPNDPANESRVLLLLQKHGFIKLRAGIDPLTGTNATPIDITENPKKWKF 186 Query: 174 RALEAATLPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSA 233 ++AA LPR L+ +D + +N +YA +A LNP +DSL +E +SPYA L+ RE ++ Sbjct: 187 VEIDAAQLPRTLDDLDASAVNADYAAKAGLNPARDSLVVESGDSPYACLIAVREKDRAQP 246 Query: 234 AIKKLAAALNTPDVKKFIEEKYKGAVVPAF 263 + KL A +P+VK++IE ++KG ++ A+ Sbjct: 247 WLGKLVQAYQSPEVKRYIETEFKGGILAAW 276 >gi|300311120|ref|YP_003775212.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 183 bits (465), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 110/268 (41%), Positives = 160/268 (59%), Gaps = 9/268 (3%) Query: 4 KLIPHLVAGAALAFSVG---AHAADKLVI-AATPVPHAE-ILEHIKPALAKEGIELQVKV 58 K I L A AL+ ++ A DK I V AE I + +K A++G+E+Q V Sbjct: 8 KNIKRLAAIGALSLALVPQLGQAQDKQKIKVGISVGSAEAIFDVVKKVAARDGLEIQTVV 67 Query: 59 FTDYIQPAAQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHVEPFAAYSQKFKKV 118 F+DY+QP A +D N F H+PYL+ + I VPV P YS+KF+ V Sbjct: 68 FSDYLQPNAALAAGDLDANAFQHRPYLESQIAARGYRI-VPVGLTITAPLGIYSRKFRSV 126 Query: 119 ADIPDGATIAIPNDPSNSGRALLLLARNGLIKLK---DSSNISSTQKDIVENPKKLKFRA 175 +P GA I I NDPSN RALLLL + GLI+LK + +++T D++ENP+KLK Sbjct: 127 EQLPQGAAIGIQNDPSNGNRALLLLQKAGLIRLKPGVGENGVNATPLDVIENPRKLKLVE 186 Query: 176 LEAATLPRVLNQVDVALINTNYALEAKLNPVKDSLFIEDANSPYANLLVAREDNKDSAAI 235 L+AA LPR L+ + A IN +YA +A L+ KD++ +ED YAN++ R ++KD + Sbjct: 187 LDAAQLPRSLDDLAAASINNDYAFKAGLSLQKDTIAVEDPRGRYANIIATRAEDKDRPWV 246 Query: 236 KKLAAALNTPDVKKFIEEKYKGAVVPAF 263 +KL A ++ +V+KFIE ++KG++VPAF Sbjct: 247 RKLVQAYHSEEVRKFIETEFKGSLVPAF 274 >gi|300311637|ref|YP_003775729.1| metal ion ABC transporter periplasmic component/surface antigen protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 115 bits (287), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 89/260 (34%), Positives = 126/260 (48%), Gaps = 21/260 (8%) Query: 8 HLVAGAALAFSV-GAHAADKLVIAATPVPHAEILEHIKPALAKEGIELQVKVFTDYIQPA 66 HLV + S+ A AADKL I P + + + ++GI++QV FTD+ P Sbjct: 5 HLVFLSLTVLSLQAAQAADKLRIGVVPGAYGDSVAVAAKEAKRQGIDVQVVEFTDWTTPN 64 Query: 67 AQTNEKQVDGNFFLHQPYLDQFKKSHKNDIEVPVAKVHV-EPFAAYSQKFKKVADIPDGA 125 + +D N+F HQP+LD KN ++ A + YS K+K + +P GA Sbjct: 65 VALDAGDLDLNYFQHQPFLD--NAIQKNGYKLASAGTGILSNVGLYSLKYKSLTALPQGA 122 Query: 126 TIAIPNDPSNSGRALLLLARNGLIKLKDSSNISSTQKDIVENPKKLKFRALEAATLPRVL 185 + I NDP N GRALLLL GLIKLK + DI++NP+KL F +E L R+ Sbjct: 123 KVGIANDPVNQGRALLLLQNVGLIKLKPKVGYLGSIDDIIDNPRKLSFVEVEGPQLVRIT 182 Query: 186 NQVDVALINTNYALEAK-LNPVKDSLF--IEDANSPYANLLVAREDNKDSAAIKKLAAAL 242 VD+A ++ + AK +P + IEDA +A V R A + Sbjct: 183 PDVDLAQGYPHFIVAAKAFDPSSGLAYSGIEDAR--FAIQFVTR------------ANRV 228 Query: 243 NTPDVKKFIEEKYKGAVVPA 262 N P V+KFI+ A V A Sbjct: 229 NDPVVQKFIQIYQNSAAVRA 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 708,776 Number of extensions: 25895 Number of successful extensions: 97 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 90 Number of HSP's successfully gapped: 5 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)