BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0157.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310886|ref|YP_003774978.1| amino acid ABC transporter peri... 488 e-139 gi|300311590|ref|YP_003775682.1| amino acid ABC transporter/sign... 405 e-114 gi|300313535|ref|YP_003777627.1| amino acid ABC transporter/sign... 276 1e-75 gi|300313169|ref|YP_003777261.1| amino acid transport periplasmi... 142 2e-35 gi|300309582|ref|YP_003773674.1| amino acid ABC transporter/sign... 135 2e-33 >gi|300310886|ref|YP_003774978.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 265 Score = 488 bits (1255), Expect = e-139, Method: Compositional matrix adjust. Identities = 242/265 (91%), Positives = 250/265 (94%), Gaps = 2/265 (0%) Query: 1 MKFVKTILA--LAALATLTGTACADNLADIQKAGVVKIGVPQDFAPYGSVTSDLQLQGLD 58 M F+KT +A L +A A AD LADIQKAGVVKIGVPQDFAPYGSVTSDLQLQGLD Sbjct: 1 MNFIKTAIAASLGLMAVAVHPAMADTLADIQKAGVVKIGVPQDFAPYGSVTSDLQLQGLD 60 Query: 59 IDVAKLVAKGLGVKAELVPVPISSRMAFLQTHKIDLIISTLGKNAEREKVIDFSQGYAPY 118 IDVA+LVAKG+GVKAELVPVPISSRMAFLQTHKIDLIISTLGKNAEREKVIDFSQGYAPY Sbjct: 61 IDVARLVAKGMGVKAELVPVPISSRMAFLQTHKIDLIISTLGKNAEREKVIDFSQGYAPY 120 Query: 119 NNSVFGVAAIKVAGPADLANQTVGVARSTFEDLMLTDSVPKSTVIKRYEDNNGLISAYVS 178 NNSVFGVAAIKVAGPADLANQTVGVARSTFEDLMLTDSVPKSTVIKRYEDNNGLI+AYVS Sbjct: 121 NNSVFGVAAIKVAGPADLANQTVGVARSTFEDLMLTDSVPKSTVIKRYEDNNGLIAAYVS 180 Query: 179 GQVQIVGTGDFVAYALAEKTPNNKPLLKYIINVSTCYVGMNKGESALMAKVNEALTASKK 238 GQVQ+VGTGDFVAYALAEKTP NKPLLKYIINVSTCYVGMNKGE ALMAKVNEALTASKK Sbjct: 181 GQVQLVGTGDFVAYALAEKTPANKPLLKYIINVSTCYVGMNKGEPALMAKVNEALTASKK 240 Query: 239 SGELNGIVKKWLNVPLPEKMATTYQ 263 SGELNGIVKKWLNVPLPEK+ATTYQ Sbjct: 241 SGELNGIVKKWLNVPLPEKIATTYQ 265 >gi|300311590|ref|YP_003775682.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 405 bits (1041), Expect = e-114, Method: Compositional matrix adjust. Identities = 198/261 (75%), Positives = 227/261 (86%), Gaps = 2/261 (0%) Query: 5 KTILA--LAALATLTGTACADNLADIQKAGVVKIGVPQDFAPYGSVTSDLQLQGLDIDVA 62 KT+LA L A G A AD LADIQK GVV+IGVPQDFAP+GSV +DLQLQGLDIDVA Sbjct: 7 KTMLAVVLGLAAMQGGLARADALADIQKNGVVRIGVPQDFAPFGSVNTDLQLQGLDIDVA 66 Query: 63 KLVAKGLGVKAELVPVPISSRMAFLQTHKIDLIISTLGKNAEREKVIDFSQGYAPYNNSV 122 +L+A+ +GVK +LVPV ++R+A+LQTHK DL+ISTLG+NAEREKVIDFSQ YAPYNNSV Sbjct: 67 RLIAQKMGVKLQLVPVASANRIAYLQTHKADLVISTLGRNAEREKVIDFSQPYAPYNNSV 126 Query: 123 FGVAAIKVAGPADLANQTVGVARSTFEDLMLTDSVPKSTVIKRYEDNNGLISAYVSGQVQ 182 FGVA++KV+GPADLA++TVGVAR TF+DL LTD+ PKS IKRYEDNN +ISAYVSGQVQ Sbjct: 127 FGVASVKVSGPADLADKTVGVARGTFQDLQLTDTAPKSATIKRYEDNNAMISAYVSGQVQ 186 Query: 183 IVGTGDFVAYALAEKTPNNKPLLKYIINVSTCYVGMNKGESALMAKVNEALTASKKSGEL 242 +VGTGDFVAYALAEK PNNKP+LKYII S C VG+NKGE AL+AKVNEALTA+KKSGEL Sbjct: 187 LVGTGDFVAYALAEKAPNNKPVLKYIIQESACNVGLNKGEPALLAKVNEALTAAKKSGEL 246 Query: 243 NGIVKKWLNVPLPEKMATTYQ 263 NGIV+KWLNVPLP KMA T Q Sbjct: 247 NGIVQKWLNVPLPAKMAATLQ 267 >gi|300313535|ref|YP_003777627.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 260 Score = 276 bits (707), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 138/257 (53%), Positives = 178/257 (69%), Gaps = 2/257 (0%) Query: 1 MKFVKTILALAALATL--TGTACADNLADIQKAGVVKIGVPQDFAPYGSVTSDLQLQGLD 58 MK K +L L A A + A AD L DIQK G +++ VP DF P+GSV +DL+ QGLD Sbjct: 1 MKLTKLLLGLMAGAMMFSASVARADALDDIQKHGTLRVAVPADFPPFGSVDADLKPQGLD 60 Query: 59 IDVAKLVAKGLGVKAELVPVPISSRMAFLQTHKIDLIISTLGKNAEREKVIDFSQGYAPY 118 IDVA L+AK LGVK EL+PV ++R+ +L T K+DL+IS++GKNAEREKVIDFS YAP+ Sbjct: 61 IDVATLIAKKLGVKVELIPVSSANRIPYLTTKKVDLVISSMGKNAEREKVIDFSAAYAPF 120 Query: 119 NNSVFGVAAIKVAGPADLANQTVGVARSTFEDLMLTDSVPKSTVIKRYEDNNGLISAYVS 178 N VFG A + V ADLA +T+ V R + EDL LT VP S +KRYEDNN IS+++S Sbjct: 121 FNGVFGPADLSVKSAADLAGKTIAVTRGSVEDLELTKIVPASATVKRYEDNNSTISSFLS 180 Query: 179 GQVQIVGTGDFVAYALAEKTPNNKPLLKYIINVSTCYVGMNKGESALMAKVNEALTASKK 238 GQVQ+V TG+ VA A+ + P KP K++I S C++G+NKGE L KV+ L SKK Sbjct: 181 GQVQLVATGNVVAAAIIARNPPKKPETKFLIKDSPCFIGLNKGEKKLQDKVDAILAESKK 240 Query: 239 SGELNGIVKKWLNVPLP 255 G L+ I +KWL +PLP Sbjct: 241 DGSLSAISQKWLGMPLP 257 >gi|300313169|ref|YP_003777261.1| amino acid transport periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 142 bits (359), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 93/254 (36%), Positives = 145/254 (57%), Gaps = 12/254 (4%) Query: 12 ALATLTGTAC------ADNLADIQKAGVVKIGVPQDFAPYGSVTSDLQLQGLDIDVAKLV 65 A A + G AC AD LADI+ V + + P+G + L+ QG D++ A+L+ Sbjct: 11 AAAVVLGLACFAQAAHADALADIKARKKVLVAIDLGAPPFGMTNAKLEPQGSDVETARLL 70 Query: 66 AKGLGVKAELVPVPISSRMAFLQTHKIDLIISTLGKNAEREKVIDFSQGYAPYNNSVFGV 125 AK LGV+ E+V V +R+ FL T K D++IS+ ER+KV+ F+Q PY S F V Sbjct: 71 AKDLGVELEIVQVTGPNRIPFLMTGKADMVISSFSITPERQKVVAFTQ---PYGASQFVV 127 Query: 126 AAIK---VAGPADLANQTVGVARSTFEDLMLTDSVPKSTVIKRYEDNNGLISAYVSGQVQ 182 AA K + DL + V V R +D +LT PK T I R++D+ + +A +SGQV Sbjct: 128 AAPKGENIKSLNDLVGKRVAVVRGNMQDSLLTPIAPKGTNIVRFDDDATVTAAIISGQVD 187 Query: 183 IVGTGDFVAYALAEKTPNNKPLLKYIINVSTCYVGMNKGESALMAKVNEALTASKKSGEL 242 + T + +A A+A++ P+ K+ I +G+ KGE AL +NE ++A+ K+G+L Sbjct: 188 ALCTPNSLASAIAKQNPSKNLETKFAIKDIPYAIGLRKGEPALEKWLNEWISANLKNGKL 247 Query: 243 NGIVKKWLNVPLPE 256 GI ++W+ P+P+ Sbjct: 248 PGIYQQWVGSPMPD 261 >gi|300309582|ref|YP_003773674.1| amino acid ABC transporter/signal transduction system periplasmic binding protein [Herbaspirillum seropedicae SmR1] Length = 269 Score = 135 bits (341), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 80/237 (33%), Positives = 125/237 (52%) Query: 22 ADNLADIQKAGVVKIGVPQDFAPYGSVTSDLQLQGLDIDVAKLVAKGLGVKAELVPVPIS 81 AD L I A +++ + PY L+L G D++ A+ +AK G++ E+VP ++ Sbjct: 28 ADQLDTILAAKKLRVAIDLAVPPYSMKDDKLRLIGSDVETAQRMAKDFGLELEVVPTTLT 87 Query: 82 SRMAFLQTHKIDLIISTLGKNAEREKVIDFSQGYAPYNNSVFGVAAIKVAGPADLANQTV 141 +R+ +LQT+K D++IS ER KVIDFS YAP V ++K+ PADLA + + Sbjct: 88 NRVPYLQTNKADIVISAFSITPERAKVIDFSVPYAPILVVVGAPRSMKITSPADLAGKKI 147 Query: 142 GVARSTFEDLMLTDSVPKSTVIKRYEDNNGLISAYVSGQVQIVGTGDFVAYALAEKTPNN 201 R T D LT P S I R++D+ ++A VSGQ I T + + +K P+ Sbjct: 148 IATRGTTNDQELTKIAPSSATITRFDDDATSMTAMVSGQADIWATSPMLLKVVNQKNPSR 207 Query: 202 KPLLKYIINVSTCYVGMNKGESALMAKVNEALTASKKSGELNGIVKKWLNVPLPEKM 258 K + + VG+ KGE L K++ + + +GELN I KK+ LP ++ Sbjct: 208 DIESKLTLKANLLAVGLRKGEPRLKEKIDGWIRTNLSNGELNTIYKKYHEAELPPEI 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.132 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 703,589 Number of extensions: 27473 Number of successful extensions: 116 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 109 Number of HSP's successfully gapped: 15 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)