BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0155.p (535 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310885|ref|YP_003774977.1| methyl-accepting chemotaxis tra... 556 e-159 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 230 2e-61 gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sen... 230 3e-61 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 229 6e-61 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 224 1e-59 >gi|300310885|ref|YP_003774977.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 551 Score = 556 bits (1432), Expect = e-159, Method: Compositional matrix adjust. Identities = 321/437 (73%), Positives = 360/437 (82%), Gaps = 5/437 (1%) Query: 1 MNPLRRLRLGPRLAAGFAALLLLLVIITFIGITRMSSLDTTSSRLSGAIYPKVNAAQKMQ 60 MNPLRRLRLG RLA GFAALLLLLV+IT IGITRMSSLD TS++LS IYPKV+AAQKMQ Sbjct: 29 MNPLRRLRLGARLACGFAALLLLLVVITVIGITRMSSLDNTSTQLSAQIYPKVSAAQKMQ 88 Query: 61 YLMMNIARSASNIIILEDDAQMNSNKQSIDKDLAEYQKTLDYLRNSVSTDEGRSLVARAD 120 YLMMNIARSA NIIIL D+AQM SNKQ IDKDLAEY + L+ LR+S T+EGR ++ Sbjct: 89 YLMMNIARSARNIIILSDEAQMASNKQGIDKDLAEYARLLEQLRSSAMTEEGRQMINAVA 148 Query: 121 EQSKAYFAFTNDVVALGLKNQNVEGQRLLFGPRYKTQGELLATLQQMVTRFEGQMREASE 180 E SK YFAFTNDVVALGLKNQN EGQRLLFGPRYK+QGELLA L ++V R+EGQM++AS Sbjct: 149 EASKNYFAFTNDVVALGLKNQNEEGQRLLFGPRYKSQGELLARLTELVQRYEGQMQQASA 208 Query: 181 AAHATYVTSATILGVSAAVAVLLGLIAALAISRSITVPMADAVDAARKVANGDLTKTITD 240 AHATYVTSATILG +A VA+LLGL+AAL I+RSITVPMA+AVDAARKVANGDLTK I D Sbjct: 209 QAHATYVTSATILGGAAGVAILLGLLAALVITRSITVPMAEAVDAARKVANGDLTKDIRD 268 Query: 241 SAPDETGDLLGALREMNTALVDIVSNVRTGADTIATASQEIAAGNQDLSARTEHQASSLE 300 ++PDETG+LL ALREMN+ALVDIV+NVRTGADTIATASQEIAAGNQDLSARTEHQASSLE Sbjct: 269 NSPDETGELLVALREMNSALVDIVTNVRTGADTIATASQEIAAGNQDLSARTEHQASSLE 328 Query: 301 ETASSMEELTSTVQQNSANARQAAEMAQAASNVAGQGGAIVGDVVSTMDAIDASARKIVD 360 ETASSMEELTSTVQQNSANARQAAEMAQ+AS +AGQGG +VGDVV+TMD IDASARKIVD Sbjct: 329 ETASSMEELTSTVQQNSANARQAAEMAQSASGIAGQGGELVGDVVATMDDIDASARKIVD 388 Query: 361 IIGVIDGIAFQTNILAXWPSVR-RRPPRRSRSSSATRWRRSTRVRNWSRRPARPWMRSSR 419 IIGVIDGIAFQTNILA +V R + R + +T VR+ ++R A Sbjct: 389 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVV----ATEVRSLAQRSAAAAKEIKV 444 Query: 420 AWPRSIPSSARSAPLVK 436 S+ A LVK Sbjct: 445 LIGDSVEKVDSGAHLVK 461 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 230 bits (586), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 159/455 (34%), Positives = 247/455 (54%), Gaps = 11/455 (2%) Query: 1 MNPLRRLRLGPRLAAGFAALLLLLVIITFIGITRMSSLDTTSSRLSGAIYPKVNAAQKMQ 60 M L++ +L FAA+++L VI+ I + +++ S+ ++ P + AAQ +Q Sbjct: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 Query: 61 YLMMNIARSASNIIILEDDAQMNSNKQSIDKDLAEYQKTLDYLRNSVSTDEGRSLVARAD 120 + + S ++ A+ ++I L K +S E + L + Sbjct: 61 ASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFS 120 Query: 121 EQSKAYFAFTNDVVALGLKNQNVEGQRLLFGPRYKTQGELLATLQQMVTRFEGQMREASE 180 + Y ++ + + + E + L G KT ++ + +++ + + + + Sbjct: 121 KSFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD 180 Query: 181 AAHATYVTSAT--ILGVSAAVAVL-LGLIAALAISRSITVPMADAVDAARKVANGDLTKT 237 A Y SAT I+G+ AA+ V+ LGL A+ ++R+++ P+ +AV A +VA GDLT Sbjct: 181 MAEQVY-RSATLWIIGLLAAIVVIALGL--AVMVARAVSRPLNEAVAVAERVAQGDLTMD 237 Query: 238 ITDSAPDETGDLLGALREMNTALVDIVSNVRTGADTIATASQEIAAGNQDLSARTEHQAS 297 I ++ DETG L+ +LR MN +L IV VRTG DTI TASQEIA GN DLS+RTE QA Sbjct: 238 IRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQAG 297 Query: 298 SLEETASSMEELTSTVQQNSANARQAAEMAQAASNVAGQGGAIVGDVVSTMDAIDASARK 357 SLEETAS+MEELT+TV+QN+ NARQA ++A +AS++A QGGA+VG VV TM+ I S+ + Sbjct: 298 SLEETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESSNR 357 Query: 358 IVDIIGVIDGIAFQTNILAXWPSVR-RRPPRRSRSSSATRWRRSTRVRNWSRRPARPWMR 416 I DII VIDGIAFQTNILA +V R + R + ++ VR+ ++R A Sbjct: 358 IADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVV----ASEVRSLAQRSASAAKE 413 Query: 417 SSRAWPRSIPSSARSAPLVKSSAPASSRCTRPWRR 451 + S+ + LV+ + S RR Sbjct: 414 IKQLIDDSVEKVGAGSQLVERAGATMSEVVASVRR 448 >gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sensory transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 585 Score = 230 bits (586), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 170/403 (42%), Positives = 226/403 (56%), Gaps = 47/403 (11%) Query: 4 LRRLRLGPRLAAGF---AALLLLLVIITFIGITRMSSLDTTSSRLSGAIYPKVNAAQKMQ 60 L L++G RL GF AA+L++LV + + R+ + ++ L+ Y + M Sbjct: 3 LADLKIGTRLYMGFGLVAAILVILVSVAYTNFARLGA----ANELNVHTYEVRAQLRAMA 58 Query: 61 YLMMNIARSASNIIILEDDAQ-------MNSNKQSIDK------DLAEYQKTLDYLRNS- 106 ++NI + DA M + K +++K D Q+ L L +S Sbjct: 59 ESLINIETGQRGFSLSGIDASLEPYHLGMTTFKTALEKTRTLTSDNTLQQQRLQELSDSE 118 Query: 107 -----VSTD---EGRSLVARADEQSKAYFAFTNDVVALGLKNQNVEGQRLLFGPRYKTQG 158 + D E R V EQ A AF G ++ R+L +T+ Sbjct: 119 QRWLKSAIDPAIELRRAVREGREQMAAVVAFEQ----AGKGKSQMDSMRVLLDDLDRTES 174 Query: 159 ELLATLQQMVTRFEGQMREASEAAHATYVTSATILGVSAAVAVLLGLIAALAISRSITVP 218 LL A EAA VTS T++G A+A++L ++ A ++ +IT P Sbjct: 175 ALLG-------------ERAEEAAALRSVTSTTLIG-GGAIALMLAVLLAWWLANNITRP 220 Query: 219 MADAVDAARKVANGDLTKTITDSAPDETGDLLGALREMNTALVDIVSNVRTGADTIATAS 278 +A AV AR+VA+GDLT I + DETG LLGAL++MN +LV IVS VR G + IATAS Sbjct: 221 LALAVQLARRVASGDLTARIEVRSRDETGALLGALKDMNESLVRIVSGVRRGTEMIATAS 280 Query: 279 QEIAAGNQDLSARTEHQASSLEETASSMEELTSTVQQNSANARQAAEMAQAASNVAGQGG 338 EIA GN DLS+RTE QASSLEETASSMEELTSTVQQN+ANAR+A MAQAAS++A GG Sbjct: 281 SEIATGNLDLSSRTEQQASSLEETASSMEELTSTVQQNTANAREANRMAQAASDIASSGG 340 Query: 339 AIVGDVVSTMDAIDASARKIVDIIGVIDGIAFQTNILAXWPSV 381 +V VV TM+ I+ S+RKIVDII VIDGIAFQTNILA +V Sbjct: 341 GVVARVVDTMEEINDSSRKIVDIISVIDGIAFQTNILALNAAV 383 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 229 bits (583), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 156/381 (40%), Positives = 223/381 (58%) Query: 1 MNPLRRLRLGPRLAAGFAALLLLLVIITFIGITRMSSLDTTSSRLSGAIYPKVNAAQKMQ 60 M + +++ RL A FAAL++++ +I +G+ + + + + Y KV ++ Sbjct: 1 MMKISDMKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYVKVALGTQIT 60 Query: 61 YLMMNIARSASNIIILEDDAQMNSNKQSIDKDLAEYQKTLDYLRNSVSTDEGRSLVARAD 120 + ARS N I+ + + +D ++ + L + +T G L+A Sbjct: 61 DKVNTGARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLAEMEKRTNTPRGSELLAALK 120 Query: 121 EQSKAYFAFTNDVVALGLKNQNVEGQRLLFGPRYKTQGELLATLQQMVTRFEGQMREASE 180 + Y VV L + + E LF Q + A +++MV+ M +++E Sbjct: 121 QARADYSKERIKVVDLIHQQKRDEATAHLFDKTIPVQDKYFAAVKEMVSFQSSLMDKSAE 180 Query: 181 AAHATYVTSATILGVSAAVAVLLGLIAALAISRSITVPMADAVDAARKVANGDLTKTITD 240 A A ++ I+ V + VAVLL + A+ I+RSIT P+ +AV A VA GDLT I Sbjct: 181 RASAASSSAINIMLVLSGVAVLLSALCAMLITRSITRPLNEAVQVATAVAAGDLTVQIEA 240 Query: 241 SAPDETGDLLGALREMNTALVDIVSNVRTGADTIATASQEIAAGNQDLSARTEHQASSLE 300 ++ DETG LLGAL+ MN L IV+ VR G DTI TAS+EIA GN DLS+RTE QA +LE Sbjct: 241 TSKDETGALLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRTEQQAGALE 300 Query: 301 ETASSMEELTSTVQQNSANARQAAEMAQAASNVAGQGGAIVGDVVSTMDAIDASARKIVD 360 ETAS+MEELTSTV+QN+ NARQA +A+ AS VA QGG++VG+VV TM I+ ++RKIVD Sbjct: 301 ETASAMEELTSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQINDASRKIVD 360 Query: 361 IIGVIDGIAFQTNILAXWPSV 381 II VIDGIAFQTNILA +V Sbjct: 361 IISVIDGIAFQTNILALNAAV 381 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 224 bits (572), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 163/414 (39%), Positives = 237/414 (57%), Gaps = 15/414 (3%) Query: 4 LRRLRLGPRLAAGFAALLLLLVIITFIGITRMSSL-DTTSSRLSGAIYPKVNAAQKMQYL 62 ++ L++G RLA GF ALL+L+ ++ +G+ R+ S+ D T A+ + +A Q + Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 Query: 63 MMNIARSASNIIILEDDAQMNSNKQSIDKDLAEYQKTLDYLRNSVSTDEGRSLVARADEQ 122 N R+ + ++ +DDA ++ ID A + + +++ E + L + Sbjct: 61 KENGVRTFA-VMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKL 119 Query: 123 SKAYFAFTNDVVALGLKNQNVEGQRLLFGPRYKTQGELLATLQQMVTR---FEGQMREAS 179 AY A + + + + + K ++ V R F+ Q+ + + Sbjct: 120 RAAYRATRQSIFDVKTAGDDARAREMTDTTLVK----MMDDYADGVLRYADFQKQVIDQT 175 Query: 180 EAA-HATYVTSATILGVSAAVAVLLGLIAALAISRSITVPMADAVDAARKVANGDLTKTI 238 AA + Y + ++L A+ ++LG A ++RSIT P+ AV A VA GDLT I Sbjct: 176 AAAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRI 235 Query: 239 TDSAPDETGDLLGALREMNTALVDIVSNVRTGADTIATASQEIAAGNQDLSARTEHQASS 298 S+ DETG LL AL+ MN L+DIV VR G +TI TAS+EIA GN DLSARTE QA S Sbjct: 236 DSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGS 295 Query: 299 LEETASSMEELTSTVQQNSANARQAAEMAQAASNVAGQGGAIVGDVVSTMDAIDASARKI 358 LEETAS+ME+LTSTV+QN+ NARQA E+A +AS VA QGGA+V VV+TM AI AS++KI Sbjct: 296 LEETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKI 355 Query: 359 VDIIGVIDGIAFQTNILAXWPSVR-RRPPRRSRSSSATRWRRSTRVRNWSRRPA 411 DIIGVIDGIAFQTNILA +V R + R + ++ VR+ ++R A Sbjct: 356 ADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVV----ASEVRSLAQRSA 405 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.127 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,110,003 Number of extensions: 36753 Number of successful extensions: 382 Number of sequences better than 1.0e-08: 65 Number of HSP's gapped: 302 Number of HSP's successfully gapped: 66 Length of query: 535 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 445 Effective length of database: 1,193,720 Effective search space: 531205400 Effective search space used: 531205400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)