BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0146.p (220 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 163 8e-42 gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 139 1e-34 gi|300313074|ref|YP_003777166.1| GGDEF domain-containing protein... 136 1e-33 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 131 5e-32 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 127 7e-31 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 163 bits (413), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 91/194 (46%), Positives = 123/194 (63%), Gaps = 3/194 (1%) Query: 17 VASLGLAGTLGALLASARGRCRELHRQLREARAANDGLRQQALYDGLTGLPNRSLLEQRI 76 +A+L +A L A R +L R L AAN+ L Q L+D LT LPNR+LLE R+ Sbjct: 228 LAALTIALLTSVLDARLESRTAKLARSLA---AANEELTQMVLHDQLTRLPNRTLLEDRL 284 Query: 77 AAAIARAERAQGSFALLFLDLDGFKLVNDRHGHAAGDLLLLQIGERLGASLRHADTIARI 136 AI +A R QG FAL+F+DLDGFK +ND GH GD LL+++ +RL +S R DT+AR+ Sbjct: 285 NQAINKAAREQGHFALMFMDLDGFKAINDSLGHHVGDRLLVEVAQRLTSSSRANDTVARL 344 Query: 137 GGDEFVVLTDIVASGDIRIIRDKLTQALAAPFQIGHTMLTVSASLGHARYPDDGNSMDAL 196 GGDEFV+L D+ D + +KL +A+ PF++ L VSAS+G A YP+DG + L Sbjct: 345 GGDEFVILVDLNTPEDAMGVAEKLVEAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDL 404 Query: 197 LACADQGMYHLKRA 210 + AD MYH KR+ Sbjct: 405 VINADAAMYHTKRS 418 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 139 bits (351), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 76/161 (47%), Positives = 104/161 (64%), Gaps = 1/161 (0%) Query: 51 NDGLRQQALYDGLTGLPNRSLLEQRIAAAIARAERAQGSFALLFLDLDGFKLVNDRHGHA 110 ND L A +D LTGLPNR+LL RI A+ A R +FA+LF+DLDGFK +ND GHA Sbjct: 255 NDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKTFAVLFMDLDGFKAINDSLGHA 314 Query: 111 AGDLLLLQIGERLGASLRHADTIARIGGDEFVVLTDIVASGD-IRIIRDKLTQALAAPFQ 169 GD LL+ + +R+ LR D +ARIGGDEFVV+ ++S D + + + + AL FQ Sbjct: 315 VGDGLLVAVAQRIRQCLRGEDMVARIGGDEFVVVVGNLSSPDVVEQLSENILAALRQDFQ 374 Query: 170 IGHTMLTVSASLGHARYPDDGNSMDALLACADQGMYHLKRA 210 I L V++S+G A YP+ G+S+DAL+ AD MY K++ Sbjct: 375 IDDATLRVTSSIGIAVYPNSGDSVDALMKNADAAMYEAKQS 415 >gi|300313074|ref|YP_003777166.1| GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 738 Score = 136 bits (343), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 76/170 (44%), Positives = 107/170 (62%), Gaps = 4/170 (2%) Query: 44 LREARAANDGLRQQALYDGLTGLPNRSLLEQRIAAAIARAERAQGSFALLFLDLDGFKLV 103 + E A D +R+ AL D LT LPNR R+ + RA RA AL F+DLDGFK + Sbjct: 555 ITERLAFRDQMRELALTDVLTSLPNRRAFSDRLPEGMMRARRAHQLLALFFMDLDGFKGI 614 Query: 104 NDRHGHAAGDLLLLQIGERLGASLRHADTIARIGGDEFVV-LTDIVASGDIRIIRDKLTQ 162 NDR+GH AGD LL++ RL AS+R DT+AR+ GDEFVV L ++ + D ++ +K+ Sbjct: 615 NDRYGHEAGDELLVEFARRLQASVRDVDTVARLAGDEFVVILENLQSQQDAIMVAEKILH 674 Query: 163 ALAAPFQIGHTMLTVSASLGHARY-PD-DGN-SMDALLACADQGMYHLKR 209 A+ PF++ H+ + +S+S+G A Y PD DG + LL+ AD+ MY KR Sbjct: 675 AIREPFELTHSTVHISSSIGVAVYQPDLDGEIGPEELLSMADKAMYAAKR 724 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 131 bits (329), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 72/156 (46%), Positives = 99/156 (63%), Gaps = 1/156 (0%) Query: 54 LRQQALYDGLTGLPNRSLLEQRIAAAIARAERAQGSFALLFLDLDGFKLVNDRHGHAAGD 113 LR QA YD L+GLPNR LL + +A A++ A++F+DLD FK VND GH+ GD Sbjct: 309 LRHQANYDPLSGLPNRRLLGDMLQREVASAKKHAYPLAVVFIDLDRFKDVNDSLGHSVGD 368 Query: 114 LLLLQIGERLGASLRHADTIARIGGDEFV-VLTDIVASGDIRIIRDKLTQALAAPFQIGH 172 LL+ +R+ AS+R D +AR+ GDEFV VL + AS + I D + ALA PFQ+G Sbjct: 369 QLLVVAAQRVRASVREHDLVARLAGDEFVAVLPRLEASAALTRIGDDIVAALAEPFQLGE 428 Query: 173 TMLTVSASLGHARYPDDGNSMDALLACADQGMYHLK 208 + +SAS+G A YP D +++ L+ CADQ M+ K Sbjct: 429 HRVRISASVGIAIYPHDADTVTTLIGCADQAMFAAK 464 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 127 bits (319), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 71/168 (42%), Positives = 102/168 (60%), Gaps = 4/168 (2%) Query: 44 LREARAANDGLRQQALYDGLTGLPNRSLLEQRIAAAIARAERAQGSF-ALLFLDLDGFKL 102 + E +A +R QA +D LTGLPNR++L + + A+ R R +G+ A+ F+DLD FK Sbjct: 252 ITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRAL-RVARQKGALTAVAFVDLDQFKF 310 Query: 103 VNDRHGHAAGDLLLLQIGERLGASLRHADTIARIGGDEFVVLTDIVASGD--IRIIRDKL 160 +ND GH GD LL + +RL A LR AD +AR GGDEFV++ G+ I + ++ Sbjct: 311 INDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQRI 370 Query: 161 TQALAAPFQIGHTMLTVSASLGHARYPDDGNSMDALLACADQGMYHLK 208 A+A P+Q G V+AS+G +RYP DG +++LL AD MY K Sbjct: 371 LAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAK 418 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 556,782 Number of extensions: 19969 Number of successful extensions: 142 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 108 Number of HSP's successfully gapped: 39 Length of query: 220 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 138 Effective length of database: 1,231,600 Effective search space: 169960800 Effective search space used: 169960800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)