BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0145.p (549 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 911 0.0 gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 609 e-175 gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 89 1e-18 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 911 bits (2354), Expect = 0.0, Method: Compositional matrix adjust. Identities = 440/547 (80%), Positives = 475/547 (86%) Query: 1 MQSTYTYPRFAYQPSDEQRSGQPQRHPVVIIGAGPIGLTAALECAARGLPAVVLDDNDTV 60 M STYTYPRFAY+ SDEQR +P RHPVV+IGAGPIGLTAALECAARGLP VVLDDNDTV Sbjct: 1 MHSTYTYPRFAYRQSDEQREARPSRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTV 60 Query: 61 SIGSRAVCYAKRPLEIWDRLGVAQRMVDKGVGWQLGKVFFEDRQVYQFDLLPEAGHRMPA 120 SIGSRAVCYAKRPLEIWDRLGVA RMV+KGV W+LGKVFFE+R+VY+FDLLPE H+MPA Sbjct: 61 SIGSRAVCYAKRPLEIWDRLGVAPRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPA 120 Query: 121 MINLQQYYLEEYLVEACTAHPLIDLRWKHALVALKQDDDGATLTVETPDGSFTMQADWII 180 MINLQQYYLE+YLVEAC A PLIDLRWKH +++L QDD ATL VETPDG F M+ADW+I Sbjct: 121 MINLQQYYLEQYLVEACQAQPLIDLRWKHKMISLHQDDTHATLDVETPDGIFRMEADWVI 180 Query: 181 ACDGANSDTRKMVGAQFTGQFFQDRFLIADVIMKADFPTERWFWFDPPFHPGQSVLLHKQ 240 ACDGANSDTRKMVGA+F+GQFFQDRFLIADVIMKADFP ERWFWFDPPFH GQSVLLHKQ Sbjct: 181 ACDGANSDTRKMVGAEFSGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQ 240 Query: 241 CDNVWRIDFQLGWDADPEEEKKPENVIPRIQAMLGPEVEFELEWVSVYQFACRRIDNMRY 300 CDNVWRIDFQLGW+ADPEEEKKPE VIPRIQAMLG EVEFELEWVSVYQFACRRID MRY Sbjct: 241 CDNVWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRY 300 Query: 301 QRVLFAGDAAHQVSPFGARGANTGVQDIDNLIWKLKLVIDGQAPVSFIDSYHEERAFAAD 360 QRVLFAGDAAHQVSPFGARGANTGVQDIDNL+WKLKLVIDG+AP SFIDSYHEERA+AAD Sbjct: 301 QRVLFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDSYHEERAWAAD 360 Query: 361 DNLSQSSRSTDFITPKSRASRVLRDAVLTLAEHEPFARTLVNSGRLSTPTPYVSSSLNTS 420 DNL QS+RSTDFITPKSRAS+ LRDAVL LAE E FARTLVNSGRLSTPT YV S LNT+ Sbjct: 361 DNLRQSTRSTDFITPKSRASKALRDAVLGLAETEEFARTLVNSGRLSTPTSYVHSPLNTA 420 Query: 421 DSASFGERMVPGTPCADAPVRQGSRPGWLLSQLGEGFTVLVFGAGDFPVSLQAAGLEIPV 480 D+A FG RM PGT CADAPV + +PGWLL LG GF VL FGA ++AAG+ V Sbjct: 421 DAALFGGRMAPGTVCADAPVLREGQPGWLLESLGAGFVVLFFGASAPQAVIEAAGISARV 480 Query: 481 KTIGPAGSGALLEDVQGLAAQRYAAHEGCAYLIRPDQHVAARWSRFDPLQIAAAVNRCVG 540 T+G GSGA L D +GLAA RYAA EGC YL+RPDQHVAARW+RF IAAA+ RCVG Sbjct: 481 VTVGAPGSGAELIDREGLAALRYAASEGCVYLVRPDQHVAARWNRFHSDDIAAALARCVG 540 Query: 541 HSMVQAA 547 M AA Sbjct: 541 QEMPLAA 547 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 609 bits (1571), Expect = e-175, Method: Compositional matrix adjust. Identities = 308/547 (56%), Positives = 376/547 (68%), Gaps = 28/547 (5%) Query: 5 YTYPRFAYQPSDEQRS-GQPQRHPVVIIGAGPIGLTAALECAARGLPAVVLDDNDTVSIG 63 Y RFA+ P EQ+S G HPVVIIGAGP+GL A++ A +G+ V++DD+DT+S G Sbjct: 9 YQQQRFAWTPCAEQQSPGHAPVHPVVIIGAGPVGLACAIDLARQGIAVVLVDDDDTLSTG 68 Query: 64 SRAVCYAKRPLEIWDRLGVAQRMVDKGVGWQLGKVFFEDRQVYQFDLLPEAGHRMPAMIN 123 SRA+C+AKR L+I+DR+G QRMVDKGVGW GKVF D+ VY+FDLLPE+GH PA IN Sbjct: 69 SRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLRDQAVYEFDLLPESGHARPAFIN 128 Query: 124 LQQYYLEEYLVEACTAHPLIDLRWKHALVALKQDDDGATLTVETPDGSFTMQADWIIACD 183 LQQYY+E YL+E P I++RWK+ + A+ +D LTV+TP GS+T++A +++A D Sbjct: 129 LQQYYVEGYLLEQAQQWPHIEIRWKNKVSAIAHHEDHEALTVDTPQGSYTLKARYVVAAD 188 Query: 184 GANSDTRKMVGAQFTGQFFQDRFLIADVIMKADFPTERWFWFDPPFHPGQSVLLHKQCDN 243 G+ S RKM+G + G F+DRFLIADV M A FP ERWFWFDPPFHP QSVLLH+Q D+ Sbjct: 189 GSRSPLRKMMGLESQGHTFRDRFLIADVKMDAPFPAERWFWFDPPFHPQQSVLLHRQPDD 248 Query: 244 VWRIDFQLGWDADPEEEKKPENVIPRIQAMLGPEVEFELEWVSVYQFACRRIDNMRYQRV 303 VWRIDFQLGWDADP EK PE VIPR++A+LG EV F LEWVSVY F+C R++ R+ RV Sbjct: 249 VWRIDFQLGWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRMERFRHGRV 308 Query: 304 LFAGDAAHQVSPFGARGANTGVQDIDNLIWKLKLVIDGQAPVSFIDSYHEERAFAADDNL 363 LF GDAAH VSPFGARGAN+G+QD +NL WKL V+ G+AP S +DSY ER +AAD+NL Sbjct: 309 LFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASEREYAADENL 368 Query: 364 SQSSRSTDFITPKSRASRVLRDAVLTLAEHEPFARTLVNSGRLSTPTPYVSSSLNTSDSA 423 S+RSTDFITPKS SR RDAVL LA FAR LVNSGRLS P S LNT D A Sbjct: 369 LNSTRSTDFITPKSAVSRAFRDAVLGLAREHAFARRLVNSGRLSLPATLDQSPLNTPDQA 428 Query: 424 ------SFGERMVPGTPCADAPVRQGSRPGWLLSQLGEGFTVLVFGA-GDFP-------- 468 +F M PG PC DAP+ Q +P WLL QLG+ F +++F A P Sbjct: 429 AAPEAVAFTGPMRPGAPCCDAPLLQAGQPAWLLPQLGDRFVLMIFAAPAQLPAATRQALA 488 Query: 469 ------------VSLQAAGLEIPVKTIGPAGSGALLEDVQGLAAQRYAAHEGCAYLIRPD 516 +S QAA E AG ++D+ LAAQRY AH G YLIRPD Sbjct: 489 ALAQPDALRLLFISAQAATQEDERVLQTLAGQAIQVQDIDTLAAQRYDAHPGSCYLIRPD 548 Query: 517 QHVAARW 523 QHV+ARW Sbjct: 549 QHVSARW 555 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 88.6 bits (218), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 125/524 (23%), Positives = 207/524 (39%), Gaps = 36/524 (6%) Query: 22 QPQRHPVVIIGAGPIGLTAALECAARGLPAVVLDDNDTVSIGSRAVCYAKRPLEIWDRLG 81 Q + V+I+GAGP+G+T G+ ++++ N V RAV L + G Sbjct: 13 QVMQAQVLIVGAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAVGIDDEALRTFQSAG 72 Query: 82 VAQRMVDKGVGWQLGKVFFEDRQVYQFDLLPEAGHRMPAMINL-QQYYLEEYLVEACTAH 140 +A +V + +++ DR+ + ++ P NL Q E L Sbjct: 73 MADAIVRDVIQNVPMRMYTADRRCFA-EIQPTTREFGWYRRNLFSQPLGEATLRLGLRRF 131 Query: 141 PLIDLRWKHALVALKQDDDGATLTVETPDGS-FTMQADWIIACDGANSDTR-KMVGAQFT 198 + LR L+ L QD+ G ++ G+ T++A +++ CDG S R K++ + Sbjct: 132 EHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRSTVREKILKLPYE 191 Query: 199 GQFFQDRFLIADVIMKADFPTERWFWFDPPF-----HPGQSVLLHKQCDNVWRIDFQLGW 253 G+ ++ V+++ D D P+ P + + V R +F L Sbjct: 192 GKTHPAKW----VVIECDGDP-----LDAPYTALHCEPQRPYVCLALPYGVRRWEFMLFP 242 Query: 254 DADPEEEKKPENVIPRIQAMLGPEVEFELEWVSVYQFACRRIDNMRYQRVLFAGDAAHQV 313 D E+ PE V ++ + + VY R ++ R+ AGDAAH Sbjct: 243 GEDGEQMLAPEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHIT 302 Query: 314 SPFGARGANTGVQDIDNLIWKLKLVIDGQAPVSFIDSYHEERAFAADDNLSQSSRSTDFI 373 P+ +G N G++D NL WKL V+ G+ + SYHEER + + + Sbjct: 303 PPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEERHAHVKAMIDLADLFGAVL 362 Query: 374 TPKSRASRVLRDAVLTLAEHEPFARTLVNSGRLSTPTPYVSSSLNTSDSASFGERMVPGT 433 + K+R RD + P R V + P P SS D +V G Sbjct: 363 SQKNRLLAWCRDKFFLAIKDIPRVRDYVLQMKFK-PMPSFSSQAVMQDDVRNRSDLV-GR 420 Query: 434 PCADAPVRQGSRPGWLLSQLGEGFTVL------VFGAGD--FPVSLQAAG---LEIPVKT 482 + V G+ L L G VL V A + +QA G L+ Sbjct: 421 MFLQSLVENGAGEVMRLDDLCGGRFVLLSWRCDVLAAAEPGLRSRMQAFGCDYLQGVRSR 480 Query: 483 IGP-----AGSGALLEDVQGLAAQRYAAHEGCAYLIRPDQHVAA 521 GP AG+G +++D + + L+RPD+ +AA Sbjct: 481 SGPRGQVLAGAGVVIQDYENKLNAWFKEKGVDWVLLRPDRFIAA 524 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,920,066 Number of extensions: 87373 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 3 Length of query: 549 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 459 Effective length of database: 1,193,720 Effective search space: 547917480 Effective search space used: 547917480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)