BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0141.p (188 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310865|ref|YP_003774957.1| response regulator containing a... 193 6e-51 gi|300313993|ref|YP_003778085.1| diguanylate cyclase/phosphodies... 160 9e-41 gi|300312320|ref|YP_003776412.1| response regulator protein [Her... 157 4e-40 gi|300313030|ref|YP_003777122.1| two component response regulato... 142 2e-35 gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspi... 136 1e-33 >gi|300310865|ref|YP_003774957.1| response regulator containing a CheY-like receiver domain and a GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 554 Score = 193 bits (491), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 98/177 (55%), Positives = 126/177 (71%) Query: 1 MFELLQKQSRRDGLTGAINRRHFDELLEAEAARFARVGGAFSLLMIDVDHFKQYNDHYGH 60 ++ L+ QS++DGLT NRR+FD L AE AR R G + SL+MIDVDHFKQ+ND YGH Sbjct: 345 LYRTLEAQSQKDGLTDVFNRRYFDAALAAELARLNRTGESLSLMMIDVDHFKQFNDTYGH 404 Query: 61 VVGDVCLRNIAAAISSAAQREIDTVARYGGEEFSVLLPECGSAGAIVVAERIGAAVRAMG 120 GDVCLR +AAAIS+ A+R+ D VARYGGEEF+V+LP C A +A R+ AVR + Sbjct: 405 AAGDVCLRQVAAAISTVARRQGDIVARYGGEEFAVILPNCHPGSAQGLAHRVVQAVRGLE 464 Query: 121 IPHATSEKGWVTVSIGITSITSAEGFSYQPRELVESADKALYQAKDTGRDRAVFREF 177 I H S G VT+S+G+ S+ S + +PREL++SAD ALY+AK+ GRDRAV R F Sbjct: 465 IAHERSPMGVVTISVGVASLASGKSLLIEPRELIDSADGALYRAKEEGRDRAVQRPF 521 >gi|300313993|ref|YP_003778085.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 573 Score = 160 bits (404), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 80/170 (47%), Positives = 109/170 (64%), Gaps = 2/170 (1%) Query: 5 LQKQSRRDGLTGAINRRHFDELLEAEAARFARVGGAFSLLMIDVDHFKQYNDHYGHVVGD 64 L+ S DGLTG NRR FDE L E R R G + +LLM+DVD+FK+YND YGH+ GD Sbjct: 398 LEALSATDGLTGVANRRRFDEALANEMRRAVRSGQSLALLMLDVDYFKKYNDRYGHLAGD 457 Query: 65 VCLRNIAAAISSAAQREIDTVARYGGEEFSVLLPECGSAGAIVVAERIGAAVRAMGIPHA 124 CLR +A + +++R D VARYGGEEFSV++ EC + A+ AE I A+ + +PHA Sbjct: 458 ECLRIVARVLQKSSRRATDLVARYGGEEFSVIIAECDTTQALQQAENICQAIFELKLPHA 517 Query: 125 TSEKGWVTVSIGITSITSAEGFSYQPRELVESADKALYQAKDTGRDRAVF 174 S G+++ SIG+ + E + P ++V AD+ALY AK GR+R V Sbjct: 518 DSPFGYISASIGVAVLLPDEHTA--PEQMVRIADQALYHAKYQGRNRVVL 565 >gi|300312320|ref|YP_003776412.1| response regulator protein [Herbaspirillum seropedicae SmR1] Length = 341 Score = 157 bits (398), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 82/176 (46%), Positives = 116/176 (65%), Gaps = 4/176 (2%) Query: 2 FELLQKQSRRDGLTGAINRRHFDELLEAEAARFARVGGAFSLLMIDVDHFKQYNDHYGHV 61 FEL Q+ + DGLTG NRR+FD+ L AE R R G +LL+IDVD FK YND+YGHV Sbjct: 168 FEL-QRLTNTDGLTGIANRRYFDDYLGAEWRRARRDGLDLALLLIDVDFFKLYNDNYGHV 226 Query: 62 VGDVCLRNIAAAISSAAQREIDTVARYGGEEFSVLLPECGSAGAIVVAERIGAAVRAMGI 121 GD LR +A A+ S+ QR D AR+GGEEF+++LP G GA +A+++ V ++ + Sbjct: 227 AGDAVLRQVAEALDSSIQRPADLSARFGGEEFAMILPRTGLDGAHAIAQKVCHLVESLQV 286 Query: 122 PH-ATSEKGWVTVSIGITSITSAEGFSYQPRELVESADKALYQAKDTGRDRAVFRE 176 PH +S W+TVS+G ++ E + REL+E+AD+ LY AK GR+R V+++ Sbjct: 287 PHEGSSVSAWLTVSVGAATLVPQE--QQELRELIEAADRRLYLAKQQGRNRVVWQD 340 >gi|300313030|ref|YP_003777122.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 320 Score = 142 bits (357), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 76/159 (47%), Positives = 95/159 (59%), Gaps = 2/159 (1%) Query: 12 DGLTGAINRRHFDELLEAEAARFARVGGAFSLLMIDVDHFKQYNDHYGHVVGDVCLRNIA 71 D LTG NRR FD L + R G A SLL+ID+DHFKQYNDHYGH GD CL +A Sbjct: 148 DSLTGLFNRRKFDSELAEKWELCKRSGQAMSLLLIDIDHFKQYNDHYGHQAGDHCLAEVA 207 Query: 72 AAISSAAQREIDTVARYGGEEFSVLLPECGSAGAIVVAERIGAAVRAMGIPHATSE-KGW 130 I S R D VARYGGEEF+ +LP G AI A+ + +V + +PHA S+ W Sbjct: 208 HTIRSNFHRSYDCVARYGGEEFACILPMTGREQAIRQAQEVCHSVARLHLPHARSDTSAW 267 Query: 131 VTVSIGITSITSAEGFSYQPRELVESADKALYQAKDTGR 169 V++SIG+ ++ A + P L+ ADK LY AK GR Sbjct: 268 VSLSIGVATMI-APDHNASPVALLALADKNLYAAKANGR 305 >gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 136 bits (342), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 73/168 (43%), Positives = 104/168 (61%), Gaps = 3/168 (1%) Query: 5 LQKQSRRDGLTGAINRRHFDELLEAEAARFAR-VGGAFSLLMIDVDHFKQYNDHYGHVVG 63 L+ +R DGLT +NRR DE L+A R R +G +FS+L IDVD+FK YND YGH +G Sbjct: 343 LEALARTDGLTDLLNRRALDEALQATWERCQRNLGASFSVLFIDVDYFKAYNDTYGHKLG 402 Query: 64 DVCLRNIAAAISSAAQREIDTVARYGGEEFSVLLPECGSAGAIVVAERIGAAVRAMGIPH 123 D L+ +AA +++ R D RYGGEEF VLL + GA ++A+R+ AVR I H Sbjct: 403 DTVLKEVAATLTARLPRHTDCAGRYGGEEFVVLLEQTDLQGAQMMAQRLCDAVRERHIAH 462 Query: 124 ATSEKGWVTVSIGITSITSAEGFSYQPRELVESADKALYQAKDTGRDR 171 +S VT+S+G+ ++ + +++ +AD ALY+AK GRDR Sbjct: 463 TSSPLAVVTISVGVATLE--RDHHRRVEDVLNAADAALYRAKQAGRDR 508 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 545,233 Number of extensions: 19996 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 39 Length of query: 188 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 108 Effective length of database: 1,241,070 Effective search space: 134035560 Effective search space used: 134035560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)