BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0140.p (351 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310865|ref|YP_003774957.1| response regulator containing a... 242 3e-65 gi|300312843|ref|YP_003776935.1| response regulator containing a... 172 3e-44 gi|300313022|ref|YP_003777114.1| two component sensor histidine ... 100 2e-22 gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspi... 94 1e-20 gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspi... 83 3e-17 >gi|300310865|ref|YP_003774957.1| response regulator containing a CheY-like receiver domain and a GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 554 Score = 242 bits (618), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 115/268 (42%), Positives = 174/268 (64%) Query: 27 LEAWTSWHERTAEMEESFRATANMALALSQHADQALDEVDIVLKAVMEQVPGKPRAVAGN 86 LEAWT+W R + ES +N+ALAL QHA+Q++ E DIVL+ V E + + R+ + Sbjct: 40 LEAWTAWRAREVALSESTTDASNVALALIQHAEQSIKEADIVLETVQEHLDHEDRSASAR 99 Query: 87 ERLHNFLVRTVGEVPQLAGLFIYDEKGKWLASSQPVLETRFNNSDRDYFRHHQTHRLDSP 146 R H+F++R+V +PQLA L + DE+G+ +ASSQ + + DYF++HQ R D P Sbjct: 100 IRTHDFMIRSVASLPQLAQLTVVDEQGRTVASSQQRMVQTGSPVQDDYFQYHQHRRSDRP 159 Query: 147 FIGKPIRSKSTGHWVLTMSRRINKPDGSFGGVALATLDIQFFERFYHKFDIGQQGAILFG 206 FIG+P+RS TG LT+SRRIN DGSF GV +A +D+ +F+R+Y +F+IG G ILFG Sbjct: 160 FIGQPMRSHVTGQRTLTVSRRINHVDGSFAGVIVAEIDLAYFQRYYEQFNIGPHGVILFG 219 Query: 207 TNEGTVLYRRPLLTDSMGKSLAQSPLFRQYISTADAGQVTIRSRQDDVTRIHGFKHLGNY 266 G+++ RRPLL D +S A S R+++ A G +RS +D V RI F+ Y Sbjct: 220 LTGGSLILRRPLLADMTDRSPAMSEALRKHMEQARRGSFAMRSERDGVERISCFQQGEAY 279 Query: 267 PLFVIVAVSENEVLQSWTRHLIARALKI 294 P+F +VA++++++L+ W+RH+ R + + Sbjct: 280 PIFTVVALAKDDILEDWSRHVWVRGIGV 307 >gi|300312843|ref|YP_003776935.1| response regulator containing a CheY-like receiver and a GGDEF domains protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 172 bits (436), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 100/277 (36%), Positives = 148/277 (53%), Gaps = 2/277 (0%) Query: 10 SRAAKGFVAAVCIIIIILEAWTSWHERTAEMEESFRATANMALALSQHADQALDEVDIVL 69 +R A+ FV +C I W S R ++E + + N+A AL++HA L D +L Sbjct: 17 ARRARLFVVLICAAIAGGHLWQSMTARGVQLENNRSYSGNVAKALARHAYDVLQATDGLL 76 Query: 70 KAVMEQVPGKPRAVAGNERLHNFLVRTVGEVPQLAGLFIYDEKGK-WLASSQPVLETRFN 128 + +++ A A L L R E+PQL GL+++DE GK L SS P L N Sbjct: 77 LDMSDRIDSVGIAHARLVDLMPMLRRLSSEMPQLDGLYVFDEHGKRLLTSSAPPLAPA-N 135 Query: 129 NSDRDYFRHHQTHRLDSPFIGKPIRSKSTGHWVLTMSRRINKPDGSFGGVALATLDIQFF 188 NSDRDYF H+ H P +GK + S+STG W++ MSRR+N PDGSF GV LATLDI F Sbjct: 136 NSDRDYFIWHRDHDDVGPHVGKALLSRSTGRWIIPMSRRLNHPDGSFAGVLLATLDINHF 195 Query: 189 ERFYHKFDIGQQGAILFGTNEGTVLYRRPLLTDSMGKSLAQSPLFRQYISTADAGQVTIR 248 + Y +IG+ G+I +GT+L R P + + +Q LF + + + G + + Sbjct: 196 NQLYRTVEIGKGGSIALLLRDGTLLTRMPFDPGYINRDFSQGRLFLEGLPHSPFGYLEVV 255 Query: 249 SRQDDVTRIHGFKHLGNYPLFVIVAVSENEVLQSWTR 285 S D R ++ + YPL ++V ++ E L W + Sbjct: 256 SPLDARQRFLSYQAVDKYPLVLVVTLAREEALADWKQ 292 >gi|300313022|ref|YP_003777114.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 100 bits (249), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 60/179 (33%), Positives = 96/179 (53%), Gaps = 3/179 (1%) Query: 108 IYDEKGKWLASSQPVLETRFNNSDRDYFRHHQTHRLDSPFIGKPIRSKSTGHWVLTMSRR 167 I D +G + SS+P T N +DR++ + H D FI KP+ + + W L ++RR Sbjct: 113 IVDAQGDVVLSSKPF--TPVNLADREHVQVHMQGGQDQLFISKPVLGRVSKKWSLQITRR 170 Query: 168 INKPDGSFGGVALATLDIQFFERFYHKFDIGQQGAILFGTNEGTVLYRRPLLTDSMGKSL 227 IN+PDG FGGV + ++D Q+F R YH+ D+G G I +G RR D+MG+ + Sbjct: 171 INRPDGGFGGVVVVSMDPQYFTRLYHQIDVGHHGVITLVGADGVARVRRTGDMDAMGEQV 230 Query: 228 AQSPLFRQYISTADAGQVTIRSRQDDVTRIHGFKHLGNYPLFVIVAVSENEVLQSWTRH 286 A ++ ++ +G + S+ D R++ F+ L +YPLF V + E L + R Sbjct: 231 AGGKVYAAMLAQG-SGTIEAVSKIDGRERLYAFQKLRDYPLFAAVGIDLKERLAPYYRE 288 >gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 94.4 bits (233), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 64/239 (26%), Positives = 114/239 (47%), Gaps = 13/239 (5%) Query: 84 AGNERLHNFLVRTVGEVPQLAGLFIYDEKGKWLASSQ----PVLETRFNNSDRDYFRHHQ 139 A +E +N+L + + L I D G +S+ P N S+R YF H+ Sbjct: 80 ASDEERYNYLFGSTAAAHHITALAILDADGYVRVTSRRSASP--SVVVNYSERTYFTVHR 137 Query: 140 THRLDSPFIGKPIRSKSTGHW-VLTMSRRINKPDGSFGGVALATLDIQFFERFYHKFDIG 198 FI +PI++ V+ +S+R+++ DGSF GV L LD+ +F + +G Sbjct: 138 DSADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGVVLMALDMSYFRDLFTGLTLG 197 Query: 199 QQGAILFGTNEGTVLYRRPLLTDSMGKSLAQSPLFRQY--ISTADAGQVTIRSRQDDVTR 256 + G + +++G R P S+G+ + +P F++ + + G R+ D V R Sbjct: 198 EDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPNFQRLRAMLVDNDGNFFARAHTDGVER 257 Query: 257 IHGFKHLGNYPLFVIVAVSENEVLQSWTRHLIARALKIKVTRARAGDYLAAARHLVRRQ 315 ++ F+ + + PL V++ SE + ++W L + L + + A AG L LVRR+ Sbjct: 258 LYTFRRIPDAPLIVLLGRSERFIFKTWYDTLYSVLLLLIIFTALAGVLLM----LVRRE 312 >gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 83.2 bits (204), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 67/295 (22%), Positives = 143/295 (48%), Gaps = 24/295 (8%) Query: 17 VAAVCIIIIILEAWTSWHERTAEMEESFRATANMALALSQHA-DQALDEVDIVLKAVME- 74 + A+ +++++ + ++ ++ + +S + A+ +SQ A + L+ + + L + Sbjct: 32 ILAIAVLMVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNLEMLSLSLDTLAYR 91 Query: 75 -QVPGKPRAVAGNERLHNFLVRTVGEVPQLAGLFIYDEKGKWLASSQPVLETRFNNS--- 130 Q P R + +R + +L + V +A + + D G+ SS+ R N + Sbjct: 92 FQHPLSARRLNDAQR-YAYLFGSTTNVSHIAVMAVLDADGRVQVSSRL---GRGNGAPHY 147 Query: 131 -DRDYFRHHQTHRLDSPFIG----KPIRSKSTGH--WVLTMSRRINKPDGSFGGVALATL 183 DR YF H+ DSP +G +P+++ S G+ V+ +S+R++ DGSF GV + L Sbjct: 148 ADRAYFIAHR----DSPTVGLYVSRPMQA-SLGNELQVVVLSKRLSNEDGSFAGVVVMAL 202 Query: 184 DIQFFERFYHKFDIGQQGAILFGTNEGTVLYRRPLLTDSMGKSLAQSPLFRQY--ISTAD 241 D+ +F + +G +G I ++EG R P +G+ ++Q+ F++ + + + Sbjct: 203 DLAYFRELFEGLALGDEGIISLYSDEGIAYMRVPYRETIIGRDISQTGNFQRLRAMLSEE 262 Query: 242 AGQVTIRSRQDDVTRIHGFKHLGNYPLFVIVAVSENEVLQSWTRHLIARALKIKV 296 G + D V R++ F+ + PL V V +E + ++W + A + + + Sbjct: 263 EGSFFAHATNDGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAI 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 993,363 Number of extensions: 39142 Number of successful extensions: 121 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 7 Length of query: 351 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 265 Effective length of database: 1,212,660 Effective search space: 321354900 Effective search space used: 321354900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)