BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0138.p (658 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 1300 0.0 gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 409 e-115 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 182 7e-47 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 160 3e-40 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 119 9e-28 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 1300 bits (3365), Expect = 0.0, Method: Compositional matrix adjust. Identities = 629/660 (95%), Positives = 639/660 (96%), Gaps = 2/660 (0%) Query: 1 VADIETFKQENRVFAPPAELARHAAISGMDAYQALNAEFEKDYEGTWAKLAKDNLNWHKP 60 +ADIETFKQENRVFAPPAEL +HAAISGMDAY ALNAEF KDYEGTWA+LAKDNL WHKP Sbjct: 1 MADIETFKQENRVFAPPAELVKHAAISGMDAYHALNAEFAKDYEGTWARLAKDNLKWHKP 60 Query: 61 FTQTLDESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTAVIFEADDGRVTRVSYRELH 120 FTQTLDESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTAVIFE+DDG+VTRVSYRELH Sbjct: 61 FTQTLDESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELH 120 Query: 121 QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER 180 QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER Sbjct: 121 QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER 180 Query: 181 VIDAGAVAVLTSDYQVRGGKQLPLKSIVDEALAMGGCDTLKHVVVYKRAGAEINWVEGRD 240 VIDAGAVAVLT+DYQVRGGKQLPLKSIVDEAL MGGCDTLKHVVVYKRAGAEINWVEGRD Sbjct: 181 VIDAGAVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRD 240 Query: 241 LWLSDVVANQPDTCEPEWVDAEHPLFILYTSGSTGKPKGVQHSSAGYLLWAVLTMKWTFD 300 LWLSDVVANQ DTCEPEWVDAEHPLFILYTSGSTGKPKGVQH+SAGYLLWAVLTMKWTFD Sbjct: 241 LWLSDVVANQSDTCEPEWVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFD 300 Query: 301 IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDKVGAPPLFFF 360 IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWD V F Sbjct: 301 IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIF 360 Query: 361 FLG--XIRSLIKAADADEKIHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGRENCPIV 418 + IRSLIKAADADEK+HPKQYDLSSLRILGSVGEPINPEAWMWYYKNVG ENCPIV Sbjct: 361 YTAPTAIRSLIKAADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIV 420 Query: 419 DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPW 478 DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPW Sbjct: 421 DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPW 480 Query: 479 PSMIRTIWNDPERFKKSYFPEELGGNIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM 538 PSMIRTIWNDPERFKKSYFPEELGG IYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM Sbjct: 481 PSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM 540 Query: 539 GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGEEAKQIAKELRDWVA 598 GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTG+EAKQIAKELRDWVA Sbjct: 541 GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVA 600 Query: 599 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ 658 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ Sbjct: 601 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ 660 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 409 bits (1050), Expect = e-115, Method: Compositional matrix adjust. Identities = 238/632 (37%), Positives = 366/632 (57%), Gaps = 19/632 (3%) Query: 32 YQALNAEFEKDYEGTWAKLAKDNLNWHKPFTQTLDESNAPFYKWFADGQVNVSYNCLDVN 91 Y+ + + E WA+ A+ ++W +PF++TLD S PF +WF DGQ N+ +N +D + Sbjct: 3 YEQFYRQSVEQPEAFWAQEAQ-RIHWQQPFSRTLDYSRPPFARWFIDGQTNLCHNAVDRH 61 Query: 92 LENGNADKTAVIFEADDGRVTRV-SYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVE 150 L A + A+I + + RV +Y EL Q+V A +++LG+++GDRV+IYMPM E Sbjct: 62 LPE-RAQQAALIAISTETNSERVYTYAELQQEVMAMAASMQALGVQRGDRVLIYMPMIAE 120 Query: 151 GVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVLTSDYQVRGGKQLPLKSIVDE 210 V AM ACARIGA HSVVFGGF++ SL R+ DA ++++D RGGK +P K ++DE Sbjct: 121 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPYKGLLDE 180 Query: 211 ALAMGGCDTLKHVVVYKRAGAEINWVEGRDLWLSDVVANQPDTCEP-EWVDAEHPLFILY 269 A+AM +HV++ R A + RD+ + + P W+++ +ILY Sbjct: 181 AIAMAQ-HRPQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQQVPVTWLESNASSYILY 239 Query: 270 TSGSTGKPKGVQHSSAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHTYIAYGPLAV 329 TSG+TGKPKGVQ GY + +M+ F +P + Y+CT+DIGW+ GH+YI YGPL Sbjct: 240 TSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIVYGPLIA 299 Query: 330 GATQIVFEGVPTYPNAGRFWDKVGAPPLFFFFLGXIRSLIKAADADEKIHPKQYDLSSLR 389 G +++EG+P P+AG +W V + F + E + ++YD SSL+ Sbjct: 300 GMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECM--ERYDTSSLK 357 Query: 390 ILGSVGEPINPEAWMWYYKNVGRENCPIVDTFWQTETGGHMISPLPGA--TPQVPGSCTL 447 L GEP++ W G P++D +WQTE+G +IS G P GS + Sbjct: 358 ALYLAGEPLDETTSSWI---SGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLGSPGV 414 Query: 448 PLPGITAAIVDE-TGNDIPNGNGGILVVKRPW-PSMIRTIWNDPERFKKSYFPEELGGNI 505 P+PG AI+DE TG G++ ++ P P ++T++ D ERF +Y+ L + Sbjct: 415 PMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYW-RSLPREV 473 Query: 506 YLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPDET 565 Y D +R+K+ GY+ I GR DDV+NV+GHR+GT EIE ++ ++ V+E AVVG D+ Sbjct: 474 YSTFDWGIRDKD-GYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKL 532 Query: 566 TGESICAFVVLKRPR--PTGEEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGK 623 G+ AF + K+ T E+ K + E+ V K+IG + +P + F LPKTRSGK Sbjct: 533 KGQVAFAFAIPKQAYTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTRSGK 592 Query: 624 IMRRLLRVLAKGEAITQDVSTLENPAILDQLK 655 ++RR ++ + +G D+ T+E+PA L+Q++ Sbjct: 593 LLRRAIQSICEGRD-PGDLPTIEDPAALEQVR 623 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 182 bits (462), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 166/567 (29%), Positives = 256/567 (45%), Gaps = 54/567 (9%) Query: 73 YKWFADGQVNVSYNCLDVNLENGNADKTAVIFEADDGRVTRVSYRELHQKVCQFANGLKS 132 Y+W N++ D +G+ +TA+I E DG VT SY +L +FAN L++ Sbjct: 24 YEWQIPTHFNIAQAACD-RWADGSG-RTAIICEDADGSVTSYSYDQLKILSDRFANALRA 81 Query: 133 LGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVLTS 192 G+ +GDR+ IY+ +E V A ++GA +F F ++ R+ ++GAVA++T Sbjct: 82 AGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTD 141 Query: 193 DYQVRGGKQLPLKSIVDEALAMGGCDTLKHVVVYKRAGAEINWVEGRDLWLSDVVANQPD 252 S +++A G L+ +V+ +++ + D W D + P Sbjct: 142 ------------ASGMEKATLAGELPALR--LVFCVESSDLVLPQTTDFW--DRLHAAPA 185 Query: 253 TCEPEWVDAEHPLFILYTSGSTGKPKGVQHSSAGYLLWAVLTMKWTFDIKPD--DVYWCT 310 +P A+ P I+YTSG+TGK KG H+ LL + ++ + D P D +W Sbjct: 186 ELDPVLTLADDPAMIIYTSGTTGKAKGALHAHR-VLLGHLPGVEVSHDSFPQEGDRFWTP 244 Query: 311 ADIGWITGHTYI----AYGPLAVGATQI-VFEGVPTYPNAGRFWDK-VGAPPLFFFFLGX 364 AD WI G + Y +AV A ++ F+ + R + V PP L Sbjct: 245 ADWAWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPPTALKMLRG 304 Query: 365 IRSLIKAADADEKIHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGRENCPI-VDTFWQ 423 ++ AD SLR + S GE + + W GRE + ++ F+ Sbjct: 305 AATVRAQAD------------FSLRSVASGGETLGDDLIAW-----GREALGVTINEFYG 347 Query: 424 TETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPWPSMIR 483 ++S P V GS +PG IVDE G +P+G G + ++ P P M Sbjct: 348 QTECNLVVSSSSHCYPSVSGSMGRAVPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFL 407 Query: 484 TIWNDPERFKKSYFPEELGGNIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEI 543 W + E + E+ G+ L GD + E GY GR DDV+ +G+R+G I Sbjct: 408 RYWRNEEATR-----EKFAGDFLLTGDLGSMD-EQGYIRYLGRNDDVITSAGYRIGPAAI 461 Query: 544 ESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGEEAKQIAKELRDWVAKEIGP 603 E L+ + V AAVVG D E + AFVVLK E K EL+ V ++ Sbjct: 462 EECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDGVTPDEALK---AELQQHVRAQLAA 518 Query: 604 IAKPKEIRFGDNLPKTRSGKIMRRLLR 630 P+ I F LP T +GKIMR+ L+ Sbjct: 519 HEYPRLISFVAALPTTATGKIMRKTLK 545 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 160 bits (405), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 146/559 (26%), Positives = 235/559 (42%), Gaps = 59/559 (10%) Query: 81 VNVSYNCLDVNLENGNADKTAVIFEADDGRVTRVSYRELHQKVCQFANGLKSLGIKKGDR 140 +N C D +++ D A+ F + G + S+ ++ + AN L+ GI+ G Sbjct: 37 INACVECCDRHVKG---DNIALEFVSLHGEHQQYSFAQVRSMAARVANLLRQQGIQPGQI 93 Query: 141 VVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVLTSDYQVRGGK 200 V +P + E +A + R GA + +F F K++ R+ +GA ++T+ Sbjct: 94 VAGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVTN-------- 145 Query: 201 QLPLKSIVDEALAMGGCDTLKHVVVYKRAGAEINWVEGRDLWLSDVVANQPDTCEPEWVD 260 + + +DE T++ RAG D+ V Q D P Sbjct: 146 -VANRDKLDEIANCPQVCTVRESNDPLRAG---------DIDFRAAVDAQSDDFAPVLRS 195 Query: 261 AEHPLFILYTSGSTGKPKGVQHSSAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHT 320 + ++ TSG+TG KGV + ++V M+ + P D +W AD GW G Sbjct: 196 GADLMLMMSTSGTTGAAKGVPVPLSALQAFSVY-MREAVGLLPQDKFWNMADPGWAYGLY 254 Query: 321 YIAYGPLAVGA----TQIVFEGVPTYPNAGRF--WDKVGAPPLFFFFLGXIRSLIKAADA 374 Y GPLA+G + F TY R GAP + + + Sbjct: 255 YAIIGPLAIGHGITFNEAAFTVSGTYETIRRLGITSLAGAPTAYRMMMAAGEEAAASVKG 314 Query: 375 DEKIHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGRENCPIVDTFWQTETGGHMISPL 434 LR + S GEP+N E W+ + + PI D + QTE G + + Sbjct: 315 ------------RLRAVSSAGEPLNAEVVRWFASAL---DVPIHDHYGQTELGMVVNNHH 359 Query: 435 PGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPWPSMIRTIWNDPERFKK 494 VPGS +PG ++DE ++ G L + I P + Sbjct: 360 ALRHEVVPGSAGFAMPGYRVVVLDENHQELGANQPGELAID---------IARSPLYWFS 410 Query: 495 SYFPEE---LGGNIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANS 551 Y+ ++ + Y GD V + G + GR DDV+ +G+R+G ++ESAL+ + Sbjct: 411 GYWKQDTPAIAHGYYSTGDN-VELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHP 469 Query: 552 IVAEAAVVGKPDETTGESICAFVVLKRPRPTGEEAKQIAKELRDWVAKEIGPIAKPKEIR 611 VA+ AV+G PD E + AFVVL + + + ++L V + + A P+ I Sbjct: 470 AVADVAVIGLPDPERTEIVKAFVVLSD---QFKASDALREQLAQHVKRRLSAHAYPRAIE 526 Query: 612 FGDNLPKTRSGKIMRRLLR 630 F D LPKT SGK+ R +LR Sbjct: 527 FLDALPKTPSGKLQRFVLR 545 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 119 bits (298), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 144/538 (26%), Positives = 227/538 (42%), Gaps = 53/538 (9%) Query: 114 VSYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFS 173 +SYREL L+ LG++KG RV I MP ++ A+ A R G T V ++ Sbjct: 49 LSYRELDSLSRALGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYT 108 Query: 174 AKSLHERVIDAGAVAVLT-SDYQVRGGKQLP---LKSIVDEALA--MGGCD-TLKHVVVY 226 A+ L ++ D+GA A+ ++ K + +K IV ++ +G TL ++VV Sbjct: 109 ARELEHQLKDSGAEAIFVLENFACTLQKVVAHTNIKHIVVASMGELLGALKGTLVNLVVR 168 Query: 227 KRAGAEINWVEGRDLWLSDVVAN-QPDTCEPEWVDAEHPLFILYTSGSTGKPKGVQHSSA 285 W + S ++ + T +P + + F+ YT G+TG KG + Sbjct: 169 HVKKLVPAWSLPNAITFSQALSQARGKTLQPVTLTHDDIAFLQYTGGTTGVAKGAVLTHR 228 Query: 286 GYLLWAVLTMKWTFDI----KPDD--VYWCTADIGWITGHTYIAYGPLAVGATQIVF--- 336 + + +W KP D V+ C + I T GA ++ Sbjct: 229 NLVANVLQAEEWLTPALKAGKPIDQLVFVCALPLYHIFALTVCNMLGTREGALNLLIPNP 288 Query: 337 EGVPTYPNAGRFWDKVGAPPLFFFFLGXIRSLIKAADADEKIHPKQYDLSSLRILGSVGE 396 +P + + P + + +L+ AD + D S R G Sbjct: 289 RDIPGFVKELAKYKVNTFPAVNTLY----NALLNNADF------ARLDFSGYRCCVGGGM 338 Query: 397 PINPEAWMWYYKNVGRENCPIVDTFWQTETGGHMISPLPGATP----QVPGSCTLPLPGI 452 + + K G CPI++ + +ET SP+ A P + G+ LPLP Sbjct: 339 AVQKSVADKWKKLTG---CPIIEGYGLSET-----SPIASANPCTIDEYTGTIGLPLPST 390 Query: 453 TAAIVDETGNDIPNGNGGILVVKRPWPSMIRTIWNDPERFKKSYFPEELGGNIYLAGDGA 512 AI+D+ G +P G G + ++ P ++ W P+ K P+ + GD Sbjct: 391 EFAILDDDGQPVPLGQPGEIAIR--GPQVMPGYWQRPDETAKVMTPD----GFFKTGDIG 444 Query: 513 VRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPDETTGESICA 572 V + E GY I R D++ VSG + E+E + + V E A VG PDE TGE++ Sbjct: 445 VMD-ERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEAVKL 503 Query: 573 FVVLKRPRPTGEEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLR 630 FVV K + A +L D+ ++ KPK I F D LPKT GKI+RR LR Sbjct: 504 FVVRK-------DKTLSAAQLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 554 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,230,381 Number of extensions: 100138 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 281 Number of HSP's successfully gapped: 9 Length of query: 658 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 567 Effective length of database: 1,188,985 Effective search space: 674154495 Effective search space used: 674154495 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)