BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0125.p (519 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310865|ref|YP_003774957.1| response regulator containing a... 601 e-173 gi|300312843|ref|YP_003776935.1| response regulator containing a... 313 2e-86 gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspi... 236 3e-63 gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspi... 201 1e-52 gi|300313993|ref|YP_003778085.1| diguanylate cyclase/phosphodies... 168 9e-43 >gi|300310865|ref|YP_003774957.1| response regulator containing a CheY-like receiver domain and a GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 554 Score = 601 bits (1549), Expect = e-173, Method: Compositional matrix adjust. Identities = 310/499 (62%), Positives = 390/499 (78%), Gaps = 2/499 (0%) Query: 10 SKTAKLFVVVVCLIIVVLEAWSAWRAYEVATDEAIRNASNMALALTQHADQSFKEIDVVL 69 S+TA LFVVVVC++I++LEAW+AWRA EVA E+ +ASN+ALAL QHA+QS KE D+VL Sbjct: 23 SRTALLFVVVVCVVILLLEAWTAWRAREVALSESTTDASNVALALIQHAEQSIKEADIVL 82 Query: 70 LGIQEHMEYDGPGVAGRRRTHEFMVASVAAVPQLAGLMVYDETGLAIGTSQATLEH--RY 127 +QEH++++ + R RTH+FM+ SVA++PQLA L V DE G + +SQ + Sbjct: 83 ETVQEHLDHEDRSASARIRTHDFMIRSVASLPQLAQLTVVDEQGRTVASSQQRMVQTGSP 142 Query: 128 IQDDYFLHHQRLRTDQAYIGKPMRSRTNGQWIVTMSRRVNHADGSFAGVVVADVDLSHFQ 187 +QDDYF +HQ R+D+ +IG+PMRS GQ +T+SRR+NH DGSFAGV+VA++DL++FQ Sbjct: 143 VQDDYFQYHQHRRSDRPFIGQPMRSHVTGQRTLTVSRRINHVDGSFAGVIVAEIDLAYFQ 202 Query: 188 RYYEQFAIGRDGAILFGLNEGTVIYRQPLLSYSIGLDISKSALVTNYLSKAEQGSFELRS 247 RYYEQF IG G ILFGL G++I R+PLL+ + S + ++ +A +GSF +RS Sbjct: 203 RYYEQFNIGPHGVILFGLTGGSLILRRPLLADMTDRSPAMSEALRKHMEQARRGSFAMRS 262 Query: 248 PRDDIVRLTSFQHCSLYPLFVAVALAKDDVLEDWTRRVWIRGIAVTLLLIIIAYGGNRLV 307 RD + R++ FQ YP+F VALAKDD+LEDW+R VW+RGI V LLL +I +GG RLV Sbjct: 263 ERDGVERISCFQQGEAYPIFTVVALAKDDILEDWSRHVWVRGIGVVLLLAVICFGGKRLV 322 Query: 308 AQVAGRENAELEAITARTELERLYRTLEAQSQKDGLTDVFNRRYFDAALTAELARLNRSG 367 VAGRE AE +AI AR ELE LYRTLEAQSQKDGLTDVFNRRYFDAAL AELARLNR+G Sbjct: 323 VLVAGRERAEAQAIAARQELESLYRTLEAQSQKDGLTDVFNRRYFDAALAAELARLNRTG 382 Query: 368 ESLSLIMVDVDHFKRYNDTYGHAAGDICLKQVAAALNGVARRQGDIVARYGGEEFALILP 427 ESLSL+M+DVDHFK++NDTYGHAAGD+CL+QVAAA++ VARRQGDIVARYGGEEFA+ILP Sbjct: 383 ESLSLMMIDVDHFKQFNDTYGHAAGDVCLRQVAAAISTVARRQGDIVARYGGEEFAVILP 442 Query: 428 NCNAASASAISSRLVQAVRALNIPHEGSSVGHVTISVGAASLASGKGAQVDARELIDTAD 487 NC+ SA ++ R+VQAVR L I HE S +G VTISVG ASLASGK ++ RELID+AD Sbjct: 443 NCHPGSAQGLAHRVVQAVRGLEIAHERSPMGVVTISVGVASLASGKSLLIEPRELIDSAD 502 Query: 488 AALYRAKEQGRDRAIQHDY 506 ALYRAKE+GRDRA+Q + Sbjct: 503 GALYRAKEEGRDRAVQRPF 521 >gi|300312843|ref|YP_003776935.1| response regulator containing a CheY-like receiver and a GGDEF domains protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 313 bits (802), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 200/495 (40%), Positives = 286/495 (57%), Gaps = 6/495 (1%) Query: 10 SKTAKLFVVVVCLIIVVLEAWSAWRAYEVATDEAIRNASNMALALTQHADQSFKEIDVVL 69 ++ A+LFVV++C I W + A V + + N+A AL +HA + D +L Sbjct: 17 ARRARLFVVLICAAIAGGHLWQSMTARGVQLENNRSYSGNVAKALARHAYDVLQATDGLL 76 Query: 70 LGIQEHMEYDGPGVAGRRRTHEF-MVASVAA-VPQLAGLMVYDETGLAIGTSQAT-LEHR 126 L + + + D G+A R M+ +++ +PQL GL V+DE G + TS A L Sbjct: 77 LDMSDRI--DSVGIAHARLVDLMPMLRRLSSEMPQLDGLYVFDEHGKRLLTSSAPPLAPA 134 Query: 127 YIQD-DYFLHHQRLRTDQAYIGKPMRSRTNGQWIVTMSRRVNHADGSFAGVVVADVDLSH 185 D DYF+ H+ ++GK + SR+ G+WI+ MSRR+NH DGSFAGV++A +D++H Sbjct: 135 NNSDRDYFIWHRDHDDVGPHVGKALLSRSTGRWIIPMSRRLNHPDGSFAGVLLATLDINH 194 Query: 186 FQRYYEQFAIGRDGAILFGLNEGTVIYRQPLLSYSIGLDISKSALVTNYLSKAEQGSFEL 245 F + Y IG+ G+I L +GT++ R P I D S+ L L + G E+ Sbjct: 195 FNQLYRTVEIGKGGSIALLLRDGTLLTRMPFDPGYINRDFSQGRLFLEGLPHSPFGYLEV 254 Query: 246 RSPRDDIVRLTSFQHCSLYPLFVAVALAKDDVLEDWTRRVWIRGIAVTLLLIIIAYGGNR 305 SP D R S+Q YPL + V LA+++ L DW ++ ++ G AV LLL II G R Sbjct: 255 VSPLDARQRFLSYQAVDKYPLVLVVTLAREEALADWKQQTFVYGAAVLLLLAIIGVFGWR 314 Query: 306 LVAQVAGRENAELEAITARTELERLYRTLEAQSQKDGLTDVFNRRYFDAALTAELARLNR 365 LV Q+ R AE A+ A EL+ LE + +DGLT + NRR+ D L E R R Sbjct: 315 LVGQIELRLRAEDRALQAMGELQMANHRLEMLAHQDGLTGLANRRHLDTMLETEFKRAER 374 Query: 366 SGESLSLIMVDVDHFKRYNDTYGHAAGDICLKQVAAALNGVARRQGDIVARYGGEEFALI 425 SG +L+LIM+DVD FK+YND YGH AGD CL++VA L RR GD+ ARYGGEE A++ Sbjct: 375 SGAALALIMIDVDFFKQYNDLYGHQAGDECLRRVAGVLKERQRRPGDLAARYGGEEMAML 434 Query: 426 LPNCNAASASAISSRLVQAVRALNIPHEGSSVGHVTISVGAASLASGKGAQVDARELIDT 485 LP +A A A++ +L ++AL +PH G+ VG VT+SVG +L A + L+ Sbjct: 435 LPGTDAEGACAVAEKLRAGIQALELPHRGNPVGVVTVSVGVCALELLPSAARSVQALLGA 494 Query: 486 ADAALYRAKEQGRDR 500 ADAALY AK +GR++ Sbjct: 495 ADAALYEAKHEGRNQ 509 >gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 236 bits (602), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 162/505 (32%), Positives = 262/505 (51%), Gaps = 34/505 (6%) Query: 11 KTAKLFVVVV----CLIIVVLEAWSAWRAYEVATDEAIRNASNMALALTQHADQSFKEID 66 K K ++VV+ L+++ + A + +++Y+ A+ NASN+ L + ++ + + Sbjct: 23 KQLKNYLVVILAIAVLMVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNLEMLS 82 Query: 67 VVLLGIQEHMEYDGPGVAGRR----RTHEFMVASVAAVPQLAGLMVYDETGLAIGTSQ-- 120 + L + ++ ++ RR + + ++ S V +A + V D G +S+ Sbjct: 83 LSLDTLAYRFQHP---LSARRLNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQVSSRLG 139 Query: 121 -ATLEHRYIQDDYFLHHQRLRTDQAYIGKPMR-SRTNGQWIVTMSRRVNHADGSFAGVVV 178 Y YF+ H+ T Y+ +PM+ S N +V +S+R+++ DGSFAGVVV Sbjct: 140 RGNGAPHYADRAYFIAHRDSPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVV 199 Query: 179 ADVDLSHFQRYYEQFAIGRDGAILFGLNEGTVIYRQPLLSYSIGLDISKSALVTNY--LS 236 +DL++F+ +E A+G +G I +EG R P IG DIS++ + Sbjct: 200 MALDLAYFRELFEGLALGDEGIISLYSDEGIAYMRVPYRETIIGRDISQTGNFQRLRAML 259 Query: 237 KAEQGSFELRSPRDDIVRLTSFQHCSLYPLFVAVALAKDDVLEDWTRRVWIRGIAVTLLL 296 E+GSF + D + RL +F+ PL V V A++ + + W + + +++ + Sbjct: 260 SEEEGSFFAHATNDGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAIFG 319 Query: 297 IIIAYGGNRLVAQVAGRENAELEAITARTELERLYRTLEAQSQKDGLTDVFNRRYFDAAL 356 ++ A+ + ++ R AE R LEA ++ DGLTD+ NRR D AL Sbjct: 320 LLTAWLLLHVRQELRRRVTAE--------------RQLEALARTDGLTDLLNRRALDEAL 365 Query: 357 TAELARLNRS-GESLSLIMVDVDHFKRYNDTYGHAAGDICLKQVAAALNGVARRQGDIVA 415 A R R+ G S S++ +DVD+FK YNDTYGH GD LK+VAA L R D Sbjct: 366 QATWERCQRNLGASFSVLFIDVDYFKAYNDTYGHKLGDTVLKEVAATLTARLPRHTDCAG 425 Query: 416 RYGGEEFALILPNCNAASASAISSRLVQAVRALNIPHEGSSVGHVTISVGAASLASGKGA 475 RYGGEEF ++L + A ++ RL AVR +I H S + VTISVG A+L Sbjct: 426 RYGGEEFVVLLEQTDLQGAQMMAQRLCDAVRERHIAHTSSPLAVVTISVGVATLERDHHR 485 Query: 476 QVDARELIDTADAALYRAKEQGRDR 500 +V+ ++++ ADAALYRAK+ GRDR Sbjct: 486 RVE--DVLNAADAALYRAKQAGRDR 508 >gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 201 bits (510), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 147/509 (28%), Positives = 252/509 (49%), Gaps = 39/509 (7%) Query: 8 RASKTAKLFVVVVCLIIVVLEAWSAWRAYEVATDEAIRNASNMALALTQHADQSFKEIDV 67 R K L + ++ ++++ + S + Y AI A+N+ L + + ++ + + + Sbjct: 3 RLQKYLALILGLIIMMVLSICGASLYEGYRQTRISAITEANNVTLIVQRAISRNMEILSL 62 Query: 68 VL--LGIQEHMEYDGPGVAGRRRTHEFMVASVAAVPQLAGLMVYDETGLAIGTSQ----A 121 + L + D + R + ++ S AA + L + D G TS+ Sbjct: 63 AMDTLAYRYQHPLDLTHASDEER-YNYLFGSTAAAHHITALAILDADGYVRVTSRRSASP 121 Query: 122 TLEHRYIQDDYFLHHQRLRTDQAYIGKPMRSRTNGQW-IVTMSRRVNHADGSFAGVVVAD 180 ++ Y + YF H+ +I +P+++ + +V +S+R++ DGSF GVV+ Sbjct: 122 SVVVNYSERTYFTVHRDSADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGVVLMA 181 Query: 181 VDLSHFQRYYEQFAIGRDGAILFGLNEGTVIYRQPLLSYSIGLDIS--------KSALVT 232 +D+S+F+ + +G DG + ++G R P SIG +I+ ++ LV Sbjct: 182 LDMSYFRDLFTGLTLGEDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPNFQRLRAMLVD 241 Query: 233 NYLSKAEQGSFELRSPRDDIVRLTSFQHCSLYPLFVAVALAKDDVLEDWTRRVWIRGIAV 292 N G+F R+ D + RL +F+ PL V + ++ + + W ++ +V Sbjct: 242 N------DGNFFARAHTDGVERLYTFRRIPDAPLIVLLGRSERFIFKTWYDTLY----SV 291 Query: 293 TLLLIIIAYGGNRLVAQVAGRENAELEAITARTELERLYRTLEAQSQKDGLTDVFNRRYF 352 LLLII L+ V L+ + A +LE L RT DGLT + NRR Sbjct: 292 LLLLIIFTALAGVLLMLV---RRELLKRVAAEKQLEDLART-------DGLTGLLNRRSL 341 Query: 353 DAALTAELARLNRSGES-LSLIMVDVDHFKRYNDTYGHAAGDICLKQVAAALNGVARRQG 411 D AL A R RS + S + +DVD+FKRYNDTYGH GD L+ VAA L +RQ Sbjct: 342 DEALLAVWQRSQRSPTAGFSTLFIDVDYFKRYNDTYGHEMGDTVLQAVAAVLREHLQRQN 401 Query: 412 DIVARYGGEEFALILPNCNAASASAISSRLVQAVRALNIPHEGSSVGHVTISVGAASLAS 471 D+ RYGGEEF ++L + A ++ +L +A+ IPH GS +G +++S+G A++ Sbjct: 402 DLAGRYGGEEFVVLLEQTDEHGALRMAQQLCEAIYNRGIPHSGSPLGVISVSIGVATMNR 461 Query: 472 GKGAQVDARELIDTADAALYRAKEQGRDR 500 +++ ++++ AD ALY AK +GR+R Sbjct: 462 APHQRME--DVLNAADHALYIAKREGRNR 488 >gi|300313993|ref|YP_003778085.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 573 Score = 168 bits (426), Expect = 9e-43, Method: Compositional matrix adjust. Identities = 94/204 (46%), Positives = 129/204 (63%), Gaps = 7/204 (3%) Query: 304 NRLVAQVAGR--ENAEL--EAITART-ELERLYRTLEAQSQKDGLTDVFNRRYFDAALTA 358 +R ++AG+ +N E+ E + RT ELER R LEA S DGLT V NRR FD AL Sbjct: 363 SRKFNEMAGKLQQNREVLEEQVRLRTRELERANRKLEALSATDGLTGVANRRRFDEALAN 422 Query: 359 ELARLNRSGESLSLIMVDVDHFKRYNDTYGHAAGDICLKQVAAALNGVARRQGDIVARYG 418 E+ R RSG+SL+L+M+DVD+FK+YND YGH AGD CL+ VA L +RR D+VARYG Sbjct: 423 EMRRAVRSGQSLALLMLDVDYFKKYNDRYGHLAGDECLRIVARVLQKSSRRATDLVARYG 482 Query: 419 GEEFALILPNCNAASASAISSRLVQAVRALNIPHEGSSVGHVTISVGAASLASGKGAQVD 478 GEEF++I+ C+ A + + QA+ L +PH S G+++ S+G A L + Sbjct: 483 GEEFSVIIAECDTTQALQQAENICQAIFELKLPHADSPFGYISASIGVAVLLPDE--HTA 540 Query: 479 ARELIDTADAALYRAKEQGRDRAI 502 +++ AD ALY AK QGR+R + Sbjct: 541 PEQMVRIADQALYHAKYQGRNRVV 564 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,543,071 Number of extensions: 63288 Number of successful extensions: 344 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 42 Length of query: 519 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 430 Effective length of database: 1,198,455 Effective search space: 515335650 Effective search space used: 515335650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)