BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0032.0116.p
         (299 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310857|ref|YP_003774949.1| drug/metabolite transporter (DM...   499   e-142
gi|300314029|ref|YP_003778121.1| permease [Herbaspirillum serope...    59   5e-10

>gi|300310857|ref|YP_003774949.1| drug/metabolite transporter (DMT)
           superfamily permease [Herbaspirillum seropedicae SmR1]
          Length = 298

 Score =  499 bits (1284), Expect = e-142,   Method: Compositional matrix adjust.
 Identities = 276/294 (93%), Positives = 286/294 (97%)

Query: 4   MTYKSLWFPLGAVLIWAGNTVISKMSAGVIAPEDISFYRWVLAALLMSPFLARPTWAARA 63
           M YK LWFPLGAVLIWAGNTVISKMSA VIAPEDISFYRWVLAALLMSPFLAR TWAARA
Sbjct: 1   MHYKHLWFPLGAVLIWAGNTVISKMSASVIAPEDISFYRWVLAALLMSPFLARQTWAARA 60

Query: 64  QIRPYLGKILVLALLGMVLFQSLAYFAAATASATSMGIIASLMPLLTLVLSIKLLSEPPT 123
           QIRP+LGKI+VLALLGMVLFQSLAYFAAATASATSMGIIASLMPLLTLVLSIKLLSEPPT
Sbjct: 61  QIRPHLGKIVVLALLGMVLFQSLAYFAAATASATSMGIIASLMPLLTLVLSIKLLSEPPT 120

Query: 124 VGTLAGGILSLFGLAVLIGRGHPLALFDQGVVTGDLLMLLATIAYGLYGVMLRRWALPLR 183
           VGTL GGILSL GLAVLIGRGHPLALF+QGVVTGDLLMLLATIAYGLYGVMLRRWALPLR
Sbjct: 121 VGTLGGGILSLLGLAVLIGRGHPLALFEQGVVTGDLLMLLATIAYGLYGVMLRRWALPLR 180

Query: 184 TWQLLYMQVLMAVVLLFPGYVLGHHSPLTRENLPILLYAGIAASILSQFLWMRGVQYLGA 243
           TWQLLYMQVLMAVVLLFPG+VLGHHSP+TRENLPILLYAGIAASI+SQFLWMRGV YLGA
Sbjct: 181 TWQLLYMQVLMAVVLLFPGFVLGHHSPVTRENLPILLYAGIAASIISQFLWMRGVAYLGA 240

Query: 244 SRCAIFVNLMPVFTVIIAVVALGEQLHGYHAIGGLITLAGVIMAQVFKQRLRWR 297
           SRCAIFVNLMPVFTVIIAV+ALGEQLHGYHA+GG+ITL GVIMAQVF+QRLRWR
Sbjct: 241 SRCAIFVNLMPVFTVIIAVLALGEQLHGYHAVGGVITLGGVIMAQVFRQRLRWR 294


>gi|300314029|ref|YP_003778121.1| permease [Herbaspirillum
           seropedicae SmR1]
          Length = 310

 Score = 58.5 bits (140), Expect = 5e-10,   Method: Compositional matrix adjust.
 Identities = 62/230 (26%), Positives = 114/230 (49%), Gaps = 16/230 (6%)

Query: 72  ILVLALLGMVLFQSLAYFAAATASATSMGIIASLMPLLTLVLSIKLLSEPPTV---GTLA 128
           + + + LG  LF        +  +A S G+I S +P +  V+S   L E  ++   G +A
Sbjct: 73  VFLESFLGNFLFTLCMITGVSMTNAVSAGVIMSAIPAVVAVMSWIFLKEKISLRVWGAVA 132

Query: 129 GGILSLFGLAVLIGRGHPLALFDQGVVT--GDLLMLLATIAYGLYGVMLRRWALPLRTWQ 186
            G + + GL  L   GH  +    G     G+ L+  A +    Y V+ ++    L   +
Sbjct: 133 CGAVGI-GLLSLAKDGHGASAASGGQYAWLGNGLVFCAVLCEAAYAVIGKKLTAVLTPKR 191

Query: 187 LLYMQVLMAVVLLFPGYVLGHHSPLTRE--NLP-----ILLYAGIAASILSQFLWMRGVQ 239
           +  +  L  ++L+ P   +G ++ L  +   +P     +LL+ G+AAS+ + +LWM G++
Sbjct: 192 ISAVINLWGLLLMTP---MGVYTALHFDFGAVPANMWWLLLFYGLAASVWTVWLWMTGLK 248

Query: 240 YLGASRCAIFVNLMPVFTVIIAVVALGEQLHGYHAIGGLITLAGVIMAQV 289
            + A+R  +F  ++PV   ++ V+ALGE L    A    I LAG+++A +
Sbjct: 249 VVPAARAGVFTVMLPVSAALVGVLALGESLSALQAGAFAIALAGMLLATL 298


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.330    0.142    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 939,293
Number of extensions: 38188
Number of successful extensions: 221
Number of sequences better than 1.0e-08: 5
Number of HSP's gapped: 219
Number of HSP's successfully gapped: 5
Length of query: 299
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 214
Effective length of database: 1,217,395
Effective search space: 260522530
Effective search space used: 260522530
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 130 (54.7 bits)