BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0115.p (608 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 811 0.0 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 489 e-139 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 326 3e-90 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 241 9e-65 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 125 1e-29 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 811 bits (2094), Expect = 0.0, Method: Compositional matrix adjust. Identities = 407/449 (90%), Positives = 432/449 (96%), Gaps = 3/449 (0%) Query: 160 MDSTVKPCLXKSSRALALDMKVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRT 219 MDSTV + S ALALDM VDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFR+ Sbjct: 1 MDSTV---ISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRS 57 Query: 220 AICVFMLGSILCGASSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLT 279 AICVFMLGSILCG SSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLT Sbjct: 58 AICVFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLT 117 Query: 280 IPALFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVSRLDIVG 339 IPALFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQV+ LD+ G Sbjct: 118 IPALFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKG 177 Query: 340 FLLSALGCSLLMMGLATSGRHLVSGDISLACVVAGVLFLCAYVWHARRATAPLIDLALLK 399 FLLSALGCSLLMMGLAT+GRHLVSG+++L CV+AG++FL AYVWHARRA+APLIDLALLK Sbjct: 178 FLLSALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLK 237 Query: 400 IPTLRAGVFGGSLYRVGIGAIPFLLPLMLQLGFGYTPFHSGLLTCASAAGAMFMKTIAAR 459 IPTLRAGVFGGS YRVGIGAIPFLLPLMLQLGFGYTPFHSG+LTCASAAGAMFMKTIAAR Sbjct: 238 IPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAAR 297 Query: 460 FLRRYGFRRVLLVNGALGVLGLWALATFTPQTPYLVMLIILLVGGCFRSLQFTSLNAISY 519 FLRRYGFRRVLLVNGALGVLGLWA+ATFTPQTPYL+M++ILL+GGCFRSLQFTSLNAISY Sbjct: 298 FLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISY 357 Query: 520 ADVDKVRLSQATSMASVAQQLSIGMGVVLAGFALQMSNELQGHATLVAQDFWPAFLVVGA 579 ADVDK+RLSQATSMASVAQQLSIGMGVVLAGFALQMSNE+QGHATLVAQDFWPAFLVVGA Sbjct: 358 ADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGA 417 Query: 580 VSALALLMLRQLPPNAGAEMAGQQENKPA 608 V+A ALLMLRQLP +AGAEMAG+ +N+PA Sbjct: 418 VAACALLMLRQLPHDAGAEMAGRDDNRPA 446 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 489 bits (1260), Expect = e-139, Method: Compositional matrix adjust. Identities = 248/441 (56%), Positives = 323/441 (73%), Gaps = 3/441 (0%) Query: 160 MDSTVKPCLXKSSRALALDMKVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRT 219 MD+TV L S ALA D+ DPI+LKLA+T+Y+ +L VFIPISGWMAD+ G+ +F Sbjct: 1 MDATV---LATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSA 57 Query: 220 AICVFMLGSILCGASSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLT 279 A+ VFM GSILC S +L FVAARFLQGIGGAMMVPVGR++I RSV+K LVKA+SYLT Sbjct: 58 AMIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLT 117 Query: 280 IPALFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVSRLDIVG 339 +PAL GPV+GP LGGFITTYFHWRW+F INIP LGLYLA RFI + K + + LD+ G Sbjct: 118 LPALLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKG 177 Query: 340 FLLSALGCSLLMMGLATSGRHLVSGDISLACVVAGVLFLCAYVWHARRATAPLIDLALLK 399 F+L+++G +++M+GL+ G HLV G +A G + L Y H+ + PL+D LL+ Sbjct: 178 FVLTSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLR 237 Query: 400 IPTLRAGVFGGSLYRVGIGAIPFLLPLMLQLGFGYTPFHSGLLTCASAAGAMFMKTIAAR 459 IPTL A V GGSL+R+G+GA+PFLLPL LQ G G T F +G +TCASA G++F++ +A R Sbjct: 238 IPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGR 297 Query: 460 FLRRYGFRRVLLVNGALGVLGLWALATFTPQTPYLVMLIILLVGGCFRSLQFTSLNAISY 519 LRRYGFR VL+VN + L A F+ TPYL+++ I+L+GG F +LQFT LN+I+Y Sbjct: 298 VLRRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAY 357 Query: 520 ADVDKVRLSQATSMASVAQQLSIGMGVVLAGFALQMSNELQGHATLVAQDFWPAFLVVGA 579 AD+D + TS+ASV QQLS+G+GV +AG LQ+SN LQGHAT+V DFWPAFLV+G Sbjct: 358 ADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGL 417 Query: 580 VSALALLMLRQLPPNAGAEMA 600 S ++ + +LP NAG E++ Sbjct: 418 FSVASVPISGKLPHNAGLELS 438 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 326 bits (836), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 193/491 (39%), Positives = 287/491 (58%), Gaps = 9/491 (1%) Query: 123 HSRCTPPSHPHSSPLTMPIPRTFIIPLIVACALFMEQMDSTVKPCLXKSSRALALDMKVD 182 H ++ ++ ++ R ++P IVA FME +D+T+ + + +A V Sbjct: 8 HPESRGENNVATNDNSLTDARVRMLPYIVAATFFMEYLDTTI---IATALPQMAQSFGVG 64 Query: 183 PISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRTAICVFMLGSILCGASSTLPQFVA 242 P L L +T+Y+++LAVFIPISGW+ADRFGS T+F AI +F L SILCG S + +F A Sbjct: 65 PNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTA 124 Query: 243 ARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPALFGPVLGPPLGGFITTYFHW 302 AR LQG+GGAMMVPVGR++++RS K L+KA++ +T PA+ PV+GP +GGFITTY W Sbjct: 125 ARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASW 184 Query: 303 RWIFFINIPISILGLYLATRFIPDLKAEQVSRLDIVGFLLSALGCSLLMMGLATSGRHLV 362 WIF +N+P + + + LD+VGFLLS + L+ G + Sbjct: 185 HWIFLMNVPFGLAAFTAVMMVVRNEHGVSKRPLDLVGFLLSGAALTSLLYGTEIASHMES 244 Query: 363 SGDISLACVVAGVLFLCAYVWHARRATAPLIDLALLKIPTLRAGVFGGSLYRVGIGAIPF 422 + ++L V GV+ H +R PL+D + L+IPT V GSL R+G+ A+P+ Sbjct: 245 NIKVALGFVALGVVLGYGAWRHMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPY 304 Query: 423 LLPLMLQLGFGYTPFHSGLLTCASAAGAMFMKTIAARFLRRYGFRRVLLVNGALGVLGLW 482 L+PLM Q+GFG T F SGLL A+A G + MK R L+R+GFR V + N A+ + Sbjct: 305 LMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLII 364 Query: 483 ALATFTPQTPYLVMLIILLVGGCFRSLQFTSLNAISYADVDKVRLSQATSMASVAQQLSI 542 A TP TP V+L ++ G RS+QF++L ++YAD+ R S A+++ SVAQQ++I Sbjct: 365 ACGLLTPATPLPVILGVIFCYGLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTI 424 Query: 543 GMGVVLAGFALQMSNELQGHAT------LVAQDFWPAFLVVGAVSALALLMLRQLPPNAG 596 GMG+ AL+++ L+G A DF AF + G V+ ++++ R L +AG Sbjct: 425 GMGIAFGALALRIAAALRGEAIDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAG 484 Query: 597 AEMAGQQENKP 607 + + G P Sbjct: 485 SNIGGGMRAAP 495 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 241 bits (616), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 154/447 (34%), Positives = 244/447 (54%), Gaps = 12/447 (2%) Query: 150 IVACALFMEQMDST-VKPCLXKSSRALALDMKVDPI-SLKLALTSYLVSLAVFIPISGWM 207 IVA FM+ +D+T V L +R+L +P+ ++ + +Y +++A P SGW+ Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLG-----EPVLNMHPVVVAYTLTMASLTPASGWL 111 Query: 208 ADRFGSLTIFRTAICVFMLGSILCGASSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVE 267 ADRFG+ ++ AI +F LGS+LC +S+LP +AAR +QG+GG+M++PVGRL +LR+V Sbjct: 112 ADRFGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVP 171 Query: 268 KVNLVKALSYLTIPALFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDL 327 + AL+ ++I GPV+GP LGG++ W WIF IN+P+ +LG+ F+PD Sbjct: 172 GAQYIAALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDD 231 Query: 328 KAEQVSRLDIVG-FLLSALGCSLLMMGLATSGRHLVSGDISLACVVAGVLFLCAYVWHAR 386 R D++G LLSA + + H I+L V A L + Y HA Sbjct: 232 SLAGTPRFDLLGCVLLSACMVAFSLALDVPFDSHRTGWSIALFVVAA--LTVLGYWRHAS 289 Query: 387 RATAPLIDLALLKIPTLRAGVFGGSLYRVGIGAIPFLLPLMLQLGFGYTPFHSGLLTCAS 446 PL L L P G+ G + R+G A+PFLLPL+LQ+ GY+P H+G++ + Sbjct: 290 VHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPA 349 Query: 447 AAGAMFMKTIAARFLRRYGFRRVLLVNGALGVLGLWALATFTPQTPYLVMLIILLVGGCF 506 A K A ++R+G+ R LLVN A+ + + A TP P ++ L + G Sbjct: 350 AIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGAS 409 Query: 507 RSLQFTSLNAISYADVDKVRLSQATSMASVAQQLSIGMGVVLAGFALQMSNELQGHATLV 566 S+QF ++N+++ +DK S+ S+ Q L+IG+GV + G + + G AT Sbjct: 410 NSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTAT-- 467 Query: 567 AQDFWPAFLVVGAVSALALLMLRQLPP 593 F F +VGA++ + + R++ P Sbjct: 468 NASFQITFAIVGAITLASAWVFRKMDP 494 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 125 bits (314), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 120/449 (26%), Positives = 206/449 (45%), Gaps = 22/449 (4%) Query: 129 PSHPHSSPLTMPIPRTFIIPLIVACALFMEQMDSTVKPCLXKSSRALALDMKVDPISLKL 188 PS ++ P + + L V+ A+FM +DS++ + L + VD Sbjct: 7 PSQAYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISG-NLGVSVD--EGTW 63 Query: 189 ALTSYLVSLAVFIPISGWMADRFGSLTIFRTAICVFMLGSILCGASSTLPQFVAARFLQG 248 +TS+ + A+ IP++GW+ R G++ +F T++ +F+L S LCG + TLP +AAR LQG Sbjct: 64 VITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQG 123 Query: 249 IGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPALFGPVLGPPLGGFITTYFHWRWIFFI 308 + MVP+ + ++L + K AL+ + A+ PV GP LGG+IT + W WIF+I Sbjct: 124 LVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYI 183 Query: 309 NIPISIL--GLYLA---TRFIPDLKAEQVSRLDIVG--FLLSALGCSLLMMGLATSGRHL 361 NIP+ ++ G+ LA +R P K +D +G L++ + +M+ Sbjct: 184 NIPVGLIAAGVTLAIYRSRETPTHKLP----IDKIGLALLVTWVAAFQIMLDKGKDLDWF 239 Query: 362 VSGDISLACVVAGVLFLCAYVWHARRATAPLIDLALLKIPTLRAGVFGGSL-YRVGIGAI 420 S I ++A + F VW P++DL L G S+ Y V G + Sbjct: 240 ASPTIVTLGIIALIAFAYFVVWELNN-DHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNL 298 Query: 421 PFLLPLMLQLGFGYTPFHSGLLTCASAAGAMFMKTIAARFLRRYGFRRVLLVNGALGVLG 480 +LP LQ GY SGL T A+ + + RFL R+V + + Sbjct: 299 -VILPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIV 357 Query: 481 LWALATFTPQTPYLVMLIILLVGGCFRSLQFTSLNAISYADVDKVRLSQATSMASVAQQL 540 W + + Q + +++ L+ G ++ F L + + + R+ A ++S + Sbjct: 358 FWLRSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVF 417 Query: 541 SIGMGVVLAGFALQMSN-----ELQGHAT 564 +G ++ A + +L HAT Sbjct: 418 CGAVGTSISTTAWNNRSIMHHAQLTEHAT 446 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,528,305 Number of extensions: 54080 Number of successful extensions: 343 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 323 Number of HSP's successfully gapped: 15 Length of query: 608 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 517 Effective length of database: 1,188,985 Effective search space: 614705245 Effective search space used: 614705245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)