BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0114.p (510 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 804 0.0 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 376 e-105 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 309 4e-85 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 269 3e-73 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 183 3e-47 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 804 bits (2076), Expect = 0.0, Method: Compositional matrix adjust. Identities = 444/514 (86%), Positives = 467/514 (90%), Gaps = 20/514 (3%) Query: 1 MTQQDLFKLKPLLTVLAASFALAACSVGP-----------VLLRAGQNWKPAQPRDLAAD 49 MTQQDLFKLKPLL +L AS ALAACSVGP + + GQNWKPAQPRDL AD Sbjct: 1 MTQQDLFKLKPLLAMLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVAD 60 Query: 50 GKWWEMFGDPQLNALAEQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLAASAGASRG 109 GKWWEMFGDPQLNAL QVDISNQNLALAEANFRSAVALVQSSRSAYSPTL+AS Sbjct: 61 GKWWEMFGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSAS------ 114 Query: 110 RSGSAGSSSSSSNSANSSNSSGNSSSSGSVRNSYTFSLTASWEADIWGKVRRDVESSQAS 169 + S SS ++ SS+++ + SS+GSVRNSYTFSLTA+WEADIWGKVRRDVESSQAS Sbjct: 115 ---AGASRGSSGSAGASSSNAASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQAS 171 Query: 170 AQASAADLAGARLSAQAQLAQNYLQLRVLDAQQQLLEDTVQAYQRSLQVTQNQYAAGLVA 229 AQASAADLA ARLSAQAQ+AQNYLQLRVLDAQQQLLE+TVQAY+RSLQV QNQYAAG+VA Sbjct: 172 AQASAADLAAARLSAQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVA 231 Query: 230 KSDVIQAQTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAEFSLPRAPLTAVAPPVPQG 289 KSDVIQAQTQLKSAQASALDNGVTRAQVEHAIALLVGKTPA+F+LPRAPL AVAPPVPQG Sbjct: 232 KSDVIQAQTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVAPPVPQG 291 Query: 290 VPSMLLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRV 349 VPSMLLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRV Sbjct: 292 VPSMLLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRV 351 Query: 350 WSLGPQLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQDVEDNLAALRILEQEA 409 WSLGPQLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQ+VEDNLAALRILEQEA Sbjct: 352 WSLGPQLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEA 411 Query: 410 AVQGEALQAARQAVTLVTNQYKAGTVSYVNVITAQATALSAELTSLNILNRRMAASVLLA 469 AVQGEALQAARQAVTLV NQYKAGTVSYVNVITAQATALSAEL+SLNILNRRMAASVLLA Sbjct: 412 AVQGEALQAARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLA 471 Query: 470 KALGGGWDGHSLDDLGPLQQQVADKAKGLKDNAA 503 KALGGGWD SLDDLG LQQQ A+KAK LK+NAA Sbjct: 472 KALGGGWDERSLDDLGSLQQQSAEKAKALKENAA 505 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 376 bits (965), Expect = e-105, Method: Compositional matrix adjust. Identities = 241/500 (48%), Positives = 318/500 (63%), Gaps = 45/500 (9%) Query: 2 TQQDLFKLKPLLTVLAASFALAACSVGPVLLRAGQN----------WKPAQPRDLAADGK 51 TQ F+ +T L + L C+VGP R + WK A PR + Sbjct: 24 TQYLPFRATKGMTALVLAALLGGCAVGPDYARPALDLPQAYKETGPWKLAAPRQIDDHQP 83 Query: 52 WWEMFGDPQLNALAEQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLAASAGASRGRS 111 WWE +GDP LNAL +Q + +NQ + A A +R A A + +R++ PT+ A AGASR ++ Sbjct: 84 WWEAYGDPVLNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQT 143 Query: 112 GSAGSSSSSSNSANSSNSSGNSSSSGSVRNSYTFSLTASWEADIWGKVRRDVESSQASAQ 171 N++ + NSY+ L ASWEAD+WG++RR E+ +A+AQ Sbjct: 144 --------------------NTNGVQRLGNSYSVGLNASWEADLWGRIRRGAEAGEANAQ 183 Query: 172 ASAADLAGARLSAQAQLAQNYLQLRVLDAQQQLLEDTVQAYQRSLQVTQNQYAAGLVAKS 231 ASAA LA ARLS QA LAQ+YLQLRV D Q+ L TV AY RSLQ++ +QY AG +S Sbjct: 184 ASAASLAAARLSIQATLAQDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRS 243 Query: 232 DVIQAQTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAEFSLPRAP------------- 278 DV QA+TQL++AQA +D TR Q+EHAIA+L+GK PA+FSLP P Sbjct: 244 DVAQAETQLRAAQAQLIDLDDTRNQLEHAIAMLIGKAPAQFSLPALPPAAASDSAIDANA 303 Query: 279 --LTAVAPPVPQGVPSMLLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQS 336 L P +P G+PS LLERRPD+A AER A+ANA IGVA+AAYFP+LTLSASGGY S Sbjct: 304 ARLQGSLPQIPVGLPSELLERRPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNS 363 Query: 337 STFADWFTLPGRVWSLGPQLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQDVE 396 FA+ F P RVWSLG LA+++FDGGAR+A D A+A YD VA YKQTVL Q+VE Sbjct: 364 LAFANLFNTPSRVWSLGSALAESLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVE 423 Query: 397 DNLAALRILEQEAAVQGEALQAARQAVTLVTNQYKAGTVSYVNVITAQATALSAELTSLN 456 D L+ LR+L+QE+ VQ +A+Q+A+ A L QY+AGTV+Y++V+T QA +L+ + ++ Sbjct: 424 DKLSTLRVLDQESTVQAQAVQSAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVT 483 Query: 457 ILNRRMAASVLLAKALGGGW 476 +L R++ ASV L KA GGGW Sbjct: 484 LLGRQLVASVALIKATGGGW 503 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 309 bits (791), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 193/447 (43%), Positives = 271/447 (60%), Gaps = 19/447 (4%) Query: 35 GQNWKPAQPRDLAADGKWWEMFGDPQLNALAEQVDISNQNLALAEANFRSAVALVQSSRS 94 G WK AQP + A G+WW++FGD +LNAL + +NQ+L A A A AL + +R+ Sbjct: 61 GSQWKTAQPAEDIARGEWWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARA 120 Query: 95 AYSPTLAASAGASRGRSGSAGSSSSSSNSANSSNSSGNSSSSGSVRNS-YTFSLTASWEA 153 Y P + A GA+R R A S G + + + N+ Y + S+E Sbjct: 121 GYFPQVDAGVGATRQRPSPA--------------SQGLPADADTRPNTLYRAQVNVSYEV 166 Query: 154 DIWGKVRRDVESSQASAQASAADLAGARLSAQAQLAQNYLQLRVLDAQQQLLEDTVQAYQ 213 D++G+V V+++ A AQ S A L+ QA +AQ Y +R LDA L TV Q Sbjct: 167 DLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQ 226 Query: 214 RSLQVTQNQYAAGLVAKSDVIQAQTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAEFS 273 SL++ Q ++ G +++ DV +A+ +L SAQ+ AL RA EHA+A+L+G+TPAEFS Sbjct: 227 ESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFS 286 Query: 274 LPRAPLTAVAPPVPQGVPSMLLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGG 333 LP PLT +A VP G+PS LLERRPD+AAAER +A+ANA++GVAK+A+FPSL L+ G Sbjct: 287 LPPQPLTRLAVTVPPGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLG 346 Query: 334 YQSSTFADWFTLPGRVWSLGP----QLAQTIFDGGARAAATDQAIATYDGTVATYKQTVL 389 Y+S+ D F R + LGP L+ +FDGG R A D+A A+Y+ VA+Y+ TVL Sbjct: 347 YESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYRGTVL 406 Query: 390 TAFQDVEDNLAALRILEQEAAVQGEALQAARQAVTLVTNQYKAGTVSYVNVITAQATALS 449 AF++VEDNLA LRIL+Q+ Q EA+ A+ +A L QY+ G VSY +VI A T L Sbjct: 407 NAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTVLQ 466 Query: 450 AELTSLNILNRRMAASVLLAKALGGGW 476 + S+ + R ++V L +ALGGGW Sbjct: 467 QQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 269 bits (688), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 177/444 (39%), Positives = 259/444 (58%), Gaps = 13/444 (2%) Query: 38 WKPAQPRDLAADGKWWEMFGDPQLNALAEQVDISNQNLALAEANFRSAVALVQSSRSAYS 97 W+ QP D G WWE FGD QLNAL +Q NQ LA+A A A A + + +A + Sbjct: 42 WRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTGAAQT 101 Query: 98 PTLAASAGASRGRSGSAGSSSSSSNSANSSNSSGNSSSSGSVRNSYTFSLTASWEADIWG 157 P + +A A+R R SAN ++ NS + +V+N + + S+E D +G Sbjct: 102 PQVNLNARAARSRI-----------SANRPLTNYNSPNFSTVQNDFALGASVSYEVDFFG 150 Query: 158 KVRRDVESSQASAQASAADLAGARLSAQAQLAQNYLQLRVLDAQQQLLEDTVQAYQRSLQ 217 +V+R VE++ A+A+ SAADL RL A+LA NY LR LD + ++ + +++L+ Sbjct: 151 RVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVELDVVRRAIALQRQALE 210 Query: 218 VTQNQYAAGLVAKSDVIQAQTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAEFSLPRA 277 + ++ G + D+ Q Q L S G RAQ EHAIA L G FS+ + Sbjct: 211 LATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIATLTGTPAPAFSITPS 270 Query: 278 PLTAVAPP-VPQGVPSMLLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQS 336 LT + PP +P GVPS +LERRPD+AAAER +A+ANA+IGVA AAY+PS L S G S Sbjct: 271 -LTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYPSFMLQPSYGVDS 329 Query: 337 STFADWFTLPGRVWSLGPQLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQDVE 396 +A F P +WSLG +Q++FDGG A D + A Y+ TVA+Y++TVLTA Q+VE Sbjct: 330 RNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASYRRTVLTAMQEVE 389 Query: 397 DNLAALRILEQEAAVQGEALQAARQAVTLVTNQYKAGTVSYVNVITAQATALSAELTSLN 456 D + L LE+ A A+ +AR+ + + +++Y+ G Y++VITAQ + L++E + Sbjct: 390 DGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVITAQQSLLNSERQAAQ 449 Query: 457 ILNRRMAASVLLAKALGGGWDGHS 480 ++ +R+ SV L KALGG W + Sbjct: 450 LMGQRLLVSVFLIKALGGDWQSRT 473 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 183 bits (464), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 146/485 (30%), Positives = 237/485 (48%), Gaps = 29/485 (5%) Query: 8 KLKPLLTVLAASFALAACSVGPVLLRAGQNWKPAQPRDLAADGK-------WWEMFGDPQ 60 +L + L + L CS+ P R + + P D A + W + F D + Sbjct: 11 RLLHMCAALVMAGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNLGWRQFFPDQR 70 Query: 61 LNALAEQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLAASAGASRGRSGSAGSSSSS 120 L L +N++L A N A A Q +R+ P L S A+R R+ ++ S + Sbjct: 71 LQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQ 130 Query: 121 SNSANSSNSSGNSSSSGSVRNSYTFSLTA-SWEADIWGKVRRDVESSQASAQASAADLAG 179 ++ NSY L ++E D +G+VR +++ AS ++ Sbjct: 131 P----------------TISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKS 174 Query: 180 ARLSAQAQLAQNYLQLRVLDAQQQLLEDTVQAYQRSLQVTQNQYAAGLVAKSDVIQAQTQ 239 A++S AQ+AQ YL R Q +L ++T+++ Q +L + Q + G D QT Sbjct: 175 AQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTL 234 Query: 240 LKSAQASALDNGVTRAQVEHAIALLVGKTPAEFSLPRAPLTA---VAPPVPQGVPSMLLE 296 ++SA+ + RAQ E+A+ LLVGK E LP A L + + +P G+PS LL Sbjct: 235 VESARVAKTQLERQRAQAENALTLLVGKKIGE--LPPAQLLSDEKIVTDIPAGLPSDLLT 292 Query: 297 RRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQL 356 RPD+ +AE+ + +ANA IG A+AA+FP ++L+ + G SST F WS GP L Sbjct: 293 NRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSL 352 Query: 357 AQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQDVEDNLAALRILEQEAAVQGEAL 416 + IFD G +A D A + V TY++ + TAF++V D L A L+++ Q L Sbjct: 353 SLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFL 412 Query: 417 QAARQAVTLVTNQYKAGTVSYVNVITAQATALSAELTSLNILNRRMAASVLLAKALGGGW 476 A ++ + L ++K G S ++ + AQ SA+ + R+ ++ L KALGGG Sbjct: 413 AAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGL 472 Query: 477 DGHSL 481 + +++ Sbjct: 473 NENTV 477 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.123 0.341 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,190,001 Number of extensions: 41367 Number of successful extensions: 237 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 180 Number of HSP's successfully gapped: 18 Length of query: 510 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 421 Effective length of database: 1,198,455 Effective search space: 504549555 Effective search space used: 504549555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)