BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0110.p (319 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 273 1e-74 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 141 8e-35 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 86 3e-18 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 85 7e-18 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 84 1e-17 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 273 bits (699), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 138/143 (96%), Positives = 142/143 (99%) Query: 84 YKEGQVVKAGELLAEIDPRSLQAALTQAEGQLARDRALLASARLDLKRYQTLLSQDSIAS 143 YKEGQVVKAG+LLAEIDPRSLQAALTQAEGQLARDRALLASARLDLKRYQTLL+QDSIAS Sbjct: 75 YKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDLKRYQTLLAQDSIAS 134 Query: 144 QQVDTQAALVKQYEGTVKADEGSVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTSDT 203 QQVDTQ ALVKQYEGTVKADEG+VASARLQLSFTRVTAPISGRLGLRQADVGNNVTTSDT Sbjct: 135 QQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTSDT 194 Query: 204 NGLVIITQLQPITAIFSIPEDNM 226 NGLVIITQLQPITAIFSIPEDN+ Sbjct: 195 NGLVIITQLQPITAIFSIPEDNI 217 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 141 bits (355), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 81/141 (57%), Positives = 104/141 (73%) Query: 84 YKEGQVVKAGELLAEIDPRSLQAALTQAEGQLARDRALLASARLDLKRYQTLLSQDSIAS 143 ++EG+ VK G+LLA+ID R+LQA L QAE Q A+D A L +ARLDL+RY TL SQD+ Sbjct: 92 FQEGEDVKKGQLLAQIDARALQAQLAQAEAQRAKDSATLMNARLDLQRYTTLRSQDAATQ 151 Query: 144 QQVDTQAALVKQYEGTVKADEGSVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTSDT 203 QQ+DTQ ALV Q E VK DE VA A++QLS+T + APISGR+G R D GN V +D Sbjct: 152 QQLDTQKALVAQLEAAVKTDEAQVAYAKVQLSYTTINAPISGRVGARLVDPGNIVHATDA 211 Query: 204 NGLVIITQLQPITAIFSIPED 224 NGLV+I Q+ PIT +F++PE+ Sbjct: 212 NGLVVINQIDPITLVFTLPEE 232 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 86.3 bits (212), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 50/134 (37%), Positives = 75/134 (55%) Query: 82 RXYKEGQVVKAGELLAEIDPRSLQAALTQAEGQLARDRALLASARLDLKRYQTLLSQDSI 141 R +KEG VK G++L +IDP +A+L A+ LAR +A L L +RY+ L+ +++ Sbjct: 78 RVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAV 137 Query: 142 ASQQVDTQAALVKQYEGTVKADEGSVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTS 201 + Q+ D A KQ E V A + +V +A L L + VT+PISGR+G G V Sbjct: 138 SKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQG 197 Query: 202 DTNGLVIITQLQPI 215 D L ++ QL P+ Sbjct: 198 DATQLALVQQLDPV 211 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 85.1 bits (209), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 49/134 (36%), Positives = 75/134 (55%) Query: 82 RXYKEGQVVKAGELLAEIDPRSLQAALTQAEGQLARDRALLASARLDLKRYQTLLSQDSI 141 R +KEG VKAG+ L +IDP S QAA A L + A LAS++L +RY L D++ Sbjct: 88 RLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSKLKAERYAELNKIDAV 147 Query: 142 ASQQVDTQAALVKQYEGTVKADEGSVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTS 201 + Q D + ++Q + V+ + +V +A + L +T++ +PISGR+ G VT S Sbjct: 148 SKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKSAVTPGALVTAS 207 Query: 202 DTNGLVIITQLQPI 215 T L + Q+ PI Sbjct: 208 QTTALTTVQQIDPI 221 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 49/134 (36%), Positives = 76/134 (56%) Query: 82 RXYKEGQVVKAGELLAEIDPRSLQAALTQAEGQLARDRALLASARLDLKRYQTLLSQDSI 141 R ++EG VKAG++L +I+P S QAA + LA+ A + +ARL RY+ L++ ++ Sbjct: 80 RSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRYKELVAIKAV 139 Query: 142 ASQQVDTQAALVKQYEGTVKADEGSVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTS 201 + Q D A + + E V + +V ++R+ L + R+ APISGR+G G VT + Sbjct: 140 SQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSVTPGALVTAN 199 Query: 202 DTNGLVIITQLQPI 215 T L I QL PI Sbjct: 200 QTTALTTIQQLDPI 213 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 740,380 Number of extensions: 25159 Number of successful extensions: 107 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 108 Number of HSP's successfully gapped: 7 Length of query: 319 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 234 Effective length of database: 1,217,395 Effective search space: 284870430 Effective search space used: 284870430 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)