BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0106.p (460 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 887 0.0 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 236 2e-63 gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 175 6e-45 gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 131 1e-31 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 105 1e-23 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 887 bits (2291), Expect = 0.0, Method: Compositional matrix adjust. Identities = 443/465 (95%), Positives = 449/465 (96%), Gaps = 5/465 (1%) Query: 1 MKNFIGSIGNRIFFLLLGGIVVAVFATNWLAGNERRNTLRELRFQHLADRVEQIVQAVDD 60 MKNF+GSIGNRIFFLLLGGIVVAVFATNWLAGNERRNTLRELRFQHLADRVEQIVQAVD+ Sbjct: 1 MKNFLGSIGNRIFFLLLGGIVVAVFATNWLAGNERRNTLRELRFQHLADRVEQIVQAVDN 60 Query: 61 IPPAQRPIVLQAATNFGFEARMVEDMNDAIGPNAS----TSPLIEMLQARLGEDRKIAVQ 116 IPPAQRPIVLQAA+NFGFEARMVEDMNDAIGP+AS TSPL EMLQARLGEDRKIAVQ Sbjct: 61 IPPAQRPIVLQAASNFGFEARMVEDMNDAIGPDASPSPSTSPLTEMLQARLGEDRKIAVQ 120 Query: 117 RESECPPLRADDGRGPRRDTCRVIYISLHDQALMRLRLHQPGEPPALRGR-GPGGPAGLF 175 RESECPPLR DD R PRRDTCRVIYISLHDQALMRLRLHQPGEPPALR R GPGGPA LF Sbjct: 121 RESECPPLRTDDARAPRRDTCRVIYISLHDQALMRLRLHQPGEPPALRARAGPGGPASLF 180 Query: 176 GLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLPEAGPTEIRQAAHA 235 GLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPL E GPTEIRQAAHA Sbjct: 181 GLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQAAHA 240 Query: 236 FNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVTDAPLQQKLIDDLGVMHGMV 295 FNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKV+D LQQKLIDDLGVMHGMV Sbjct: 241 FNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVSDPGLQQKLIDDLGVMHGMV 300 Query: 296 REGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGSTRAFVLAQPGALRRCL 355 REGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGSTRAFV+AQPGALRRCL Sbjct: 301 REGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGSTRAFVMAQPGALRRCL 360 Query: 356 TNLIDNACKYGKSARVSIALEQQKIAIRIRDQGPGIPESELEAVFEPFYRLEASRSRDTG 415 TNLIDNACKYGKSARVSIALEQQKIAIRIRDQGPGIPESELEAVFEPFYRLEASRSRDTG Sbjct: 361 TNLIDNACKYGKSARVSIALEQQKIAIRIRDQGPGIPESELEAVFEPFYRLEASRSRDTG 420 Query: 416 GTGLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELPPMTPTKNR 460 GTGLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELPPMTP KNR Sbjct: 421 GTGLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELPPMTPAKNR 465 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 236 bits (603), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 161/473 (34%), Positives = 248/473 (52%), Gaps = 38/473 (8%) Query: 7 SIGNRIFFLLLGGIVVAVFATNWLAGNERRNTLRELRFQHLADRVEQIVQAVDDIPPAQR 66 ++ R+ +L+ G++ A T + + R T E+ + A + V +DD P QR Sbjct: 17 TLSGRLALILVAGMLAAQMLTGTIWYDMRYGTAMEMPTRLAAAHLADAVLILDDAPATQR 76 Query: 67 PIVLQAATNFGFEARM--VEDMNDAIGPN--ASTSPLI---------------------- 100 + + T F + V D D P ++SP Sbjct: 77 QALAHSLTRAQFVLSLPDVVDRADLAVPGLAPASSPTTHADQGVVDLFGTVLQQHAGPRP 136 Query: 101 -EMLQARLGEDRKIAVQRESECPPLRADDGRGPRRDTCRVIYISLHDQALMRLRLHQPGE 159 +MLQ LG D + A S LR + G R + ++L D + ++ + E Sbjct: 137 WKMLQ--LGLDDQHANAESSWWTLLRDEFPHGRFR-----VLVTLKDGSWLQADIE---E 186 Query: 160 PPALRGRGPGGPAGLFGLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHA 219 A P A + ++ L + + ++AW+ R+A +P++ +A+AA +LG +I A Sbjct: 187 GQAGMSTAPHSAAFDYFMRIYALRIFAVVLIAWMAVRLAMKPLQVMAEAAEKLGGNIRSA 246 Query: 220 PLPEAGPTEIRQAAHAFNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVTDAP 279 PL GP E+ AA AFNAMQ RI I RT LAA++HDL++P+TRM+LR E + D Sbjct: 247 PLATDGPLEVSTAAQAFNAMQRRIIDNIAERTRFLAAVSHDLRSPITRMKLRTEMLPDER 306 Query: 280 LQQKLIDDLGVMHGMVREGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGS 339 ++K DL M ++ L++ + +D E+ Q +D+DSLL+S+ D A+ G +V L G Sbjct: 307 GKEKFRKDLDEMDALISATLNVVQDIDVQERPQLIDLDSLLESLRTDQAEVGGEVTLSGH 366 Query: 340 TRAFVLAQPGALRRCLTNLIDNACKYGKSARVSIALEQQKIAIRIRDQGPGIPESELEAV 399 A + P +LRRCL NL++NA +YG+ ARV + + ++ IR+ D GPGIPE+ LE V Sbjct: 367 A-APLQGYPRSLRRCLQNLVENAVRYGQRARVQLQDDADQVCIRVDDDGPGIPEALLERV 425 Query: 400 FEPFYRLEASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELP 452 EPFYRLE SR+ TGG GLGL+IA + + H G ++L N GGL V + +P Sbjct: 426 QEPFYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGGGLRVEILIP 478 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 175 bits (444), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 103/263 (39%), Positives = 150/263 (57%), Gaps = 5/263 (1%) Query: 194 VARMATRPIRQLAQAASRLGSDIDHAPLPEAGPTEIRQAAHAFNAMQARIRRQIQHRTHM 253 V R TRP+ LA AA G LPE G E + A AF+ M+ RI+R ++ R + Sbjct: 215 VVRWTTRPLAALADAAEAFGKGETVPSLPETGSREYVKTARAFSGMRERIKRYLEDRERL 274 Query: 254 LAAITHDLQTPLTRMRLRLEKVTDAPLQQKLIDDLGVMHGMVREGLDLARSMDSSEKIQA 313 +I+HDL+TP+TR++LR E + D ++ DDL + MV+ L + D E Sbjct: 275 FVSISHDLRTPITRLKLRTELMDDDAMRTDFHDDLDELDMMVKGALQSVKDSDIHENRTE 334 Query: 314 LDIDSLLDSVCADAADAGQDVALQGSTRAFVLAQPGALRRCLTNLIDNACKYGKSARVSI 373 + +D+L+ + DA AG ++A S V+A+P AL+R + NL DNA YG+ +SI Sbjct: 335 VRLDALVLRMIRDARLAGHEIAFTPSG-INVIAKPLALKRAIGNLFDNALYYGQKVEISI 393 Query: 374 ----ALEQQKIAIRIRDQGPGIPESELEAVFEPFYRLEASRSRDTGGTGLGLTIALNIAE 429 E + I IRD GPG+PE + +F+P+ RLE R++++GG GLGL I+ NI + Sbjct: 394 RPSPGAEGDLVEIEIRDHGPGVPEDAMGTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQ 453 Query: 430 NHHGQLRLRNLAEGGLEVLLELP 452 H G+L LRN EGGL + LP Sbjct: 454 AHGGELLLRNHPEGGLMATIILP 476 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 131 bits (330), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 97/290 (33%), Positives = 151/290 (52%), Gaps = 17/290 (5%) Query: 175 FGLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLPEAGPTEIRQAAH 234 G I LFL LI A ++ + +P+ +L AA + PLPE+GPTEI +A Sbjct: 163 LGWGSIALFLSLIG--AVFISTLINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANR 220 Query: 235 AFNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLE--KVTDAPLQQKLIDDLGVMH 292 +FN M A + R R +LA I+HDL+TPL RM+L +E ++D + + DL M Sbjct: 221 SFNQMVADLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDEA-RDGMHSDLAQMD 279 Query: 293 GMVREGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQ-DVALQGSTRAFVLAQPGAL 351 ++ + LD A+ D+S ++ +D+ +L V +A+ V Q + V L Sbjct: 280 SIIGQFLDYAKPFDTS-SLETIDLADVLQDVAGNASRLSDIRVHTQITPDTQVAGNGTEL 338 Query: 352 RRCLTNLIDNACKYGKSARVSIA-------LEQQKIAIRIRDQGPGIPESELEAVFEPFY 404 R +NL++NA +YGK+ A +E ++ + + D GPG+P +E E + PF Sbjct: 339 ARVFSNLVENARRYGKTEGTDCAEIDIRSRIEGHQVVVEVADHGPGVPVNECERLLRPFT 398 Query: 405 RLEASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLAE--GGLEVLLELP 452 RL+ +R + G+GLGL I I H+G+L L+ E GL V + LP Sbjct: 399 RLDTARGQ-ANGSGLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLP 447 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 105 bits (261), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 95/283 (33%), Positives = 136/283 (48%), Gaps = 29/283 (10%) Query: 187 IAMLAWLVARMATRPIRQLA-QAASRLGSDIDHAPLPEAG-PTEIRQAAHAFNAMQARIR 244 I +L+W+ P+R+ A + A+R + + PL + G P EI+ HA N + AR++ Sbjct: 153 IGVLSWVAVSRGLAPVRKAAAEVAARDMNSLQ--PLADVGMPQEIQPLTHALNDLLARLK 210 Query: 245 RQIQHRTHMLAAITHDLQTPLTRMRLRL---EKVTDAPLQQKLIDDLG-----VMHGMVR 296 + + +A H+L+TPLT +RL+ E+ D ++ DL H +V Sbjct: 211 HATETQRAFVADAAHELRTPLTALRLQTQLAERAVDEEERRAAFADLKNGLERTTH-LVH 269 Query: 297 EGLDLARSMDSSEKIQALDIDSLLDSVCAD----AADAGQDVAL--QGST-RAF-VLAQP 348 + L LAR + +D+ +LL SV D A G D+ L QG R V Sbjct: 270 QLLTLARQEPDAMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNA 329 Query: 349 GALRRCLTNLIDNACKY---GKSARVSIALEQQ-KIAIRIRDQGPGIPESELEAVFEPFY 404 ALR NL+DNA +Y G V + L + ++D GPGI ESEL VF+ FY Sbjct: 330 DALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRFY 389 Query: 405 RL----EASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLAEG 443 R E S SR G+GLGL I IA+ H + LRN G Sbjct: 390 RAQHSQEGSGSRAAFGSGLGLAIVRQIADLHRVPVELRNTGHG 432 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,242,368 Number of extensions: 47743 Number of successful extensions: 324 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 292 Number of HSP's successfully gapped: 38 Length of query: 460 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 372 Effective length of database: 1,203,190 Effective search space: 447586680 Effective search space used: 447586680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)