BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0100.p (171 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310842|ref|YP_003774934.1| cobalamin biosynthesis transmem... 342 9e-96 gi|300311955|ref|YP_003776047.1| cobalamin biosynthesis CobD/Cbi... 59 3e-10 >gi|300310842|ref|YP_003774934.1| cobalamin biosynthesis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 342 bits (877), Expect = 9e-96, Method: Compositional matrix adjust. Identities = 169/171 (98%), Positives = 171/171 (100%) Query: 1 VFGVFFWFLMPVGPALAVMYRVAEYLSRAWNEPDHMKYEAFGRFAAQAFYWIDWIPARLT 60 VFGVFFWFLMPVGPALAVMYRVAEYLSRAWNEPDHMK+EAFGRFAAQAFYWIDWIPARLT Sbjct: 149 VFGVFFWFLMPVGPALAVMYRVAEYLSRAWNEPDHMKFEAFGRFAAQAFYWIDWIPARLT 208 Query: 61 AAAFAVVGNFEDAIYAWRNFASRWDDEAAGIILAAGGGAMGVRFGTEKEAAAGIILPADA 120 AAAFAVVGNFEDAIYAWRNFASRWDDE+AGIILAAGGGAMGVRFGTEKEAAAGIILPADA Sbjct: 209 AAAFAVVGNFEDAIYAWRNFASRWDDESAGIILAAGGGAMGVRFGTEKEAAAGIILPADA 268 Query: 121 ATVDSMPEETDGLPGDTPSPRTLQSTVGLVWRALLLWMFLLLLLSVAVWMG 171 ATVDSMPEETDGLPGDTPSPRTLQSTVGLVWRALLLWMFLLLLLSVAVWMG Sbjct: 269 ATVDSMPEETDGLPGDTPSPRTLQSTVGLVWRALLLWMFLLLLLSVAVWMG 319 >gi|300311955|ref|YP_003776047.1| cobalamin biosynthesis CobD/CbiB [Herbaspirillum seropedicae SmR1] Length = 317 Score = 58.5 bits (140), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 33/93 (35%), Positives = 52/93 (55%), Gaps = 2/93 (2%) Query: 1 VFGVFFWFLMPVGPALAVMYRVAEYLSRAWNEPDHMKYEAFGRFAAQAFYWIDWIPARLT 60 VFG FWFL+ GP A+++R++ L W + ++ FG AA+ ++W PARLT Sbjct: 156 VFGTLFWFLLAGGPG-ALLFRLSNTLDAMWGY-RNARFLYFGWAAARIDDALNWCPARLT 213 Query: 61 AAAFAVVGNFEDAIYAWRNFASRWDDEAAGIIL 93 A ++AV+G A+ W+ A W AG ++ Sbjct: 214 ALSYAVLGQRRQALACWKEQAPAWSSPNAGPVM 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.460 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 483,114 Number of extensions: 15243 Number of successful extensions: 63 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 62 Number of HSP's successfully gapped: 2 Length of query: 171 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 92 Effective length of database: 1,245,805 Effective search space: 114614060 Effective search space used: 114614060 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)