BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0091.p (381 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 730 0.0 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 336 2e-93 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 303 2e-83 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 96 5e-21 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 77 3e-15 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 730 bits (1884), Expect = 0.0, Method: Compositional matrix adjust. Identities = 349/354 (98%), Positives = 353/354 (99%) Query: 1 LFVMNELAGLAAVNALPGCEDATPETAEAILEENARFCSEVIAPLNHSADKAPSSWADGK 60 LFVMNELAGLAAVNALPGCEDATPETAEAILEENA+FCSEV+APLNHSADK PSSWADGK Sbjct: 11 LFVMNELAGLAAVNALPGCEDATPETAEAILEENAKFCSEVVAPLNHSADKEPSSWADGK 70 Query: 61 VTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALCPLLTD 120 VTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALCPLLTD Sbjct: 71 VTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALCPLLTD 130 Query: 121 GTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGTFKLF 180 GTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGT+KLF Sbjct: 131 GTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGTYKLF 190 Query: 181 GTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGSLGARNDVHCV 240 GTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGSLGARNDVHCV Sbjct: 191 GTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGSLGARNDVHCV 250 Query: 241 SIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSER 300 SIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSER Sbjct: 251 SIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSER 310 Query: 301 AYQKAVSYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALSYVA 354 AYQKAV+YARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALSYVA Sbjct: 311 AYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALSYVA 364 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 336 bits (861), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 174/362 (48%), Positives = 240/362 (66%), Gaps = 13/362 (3%) Query: 2 FVMNELAGLAAV-NALPGCEDATPETAEAILEENARFCSEVIAPLNHSADKAPSSW--AD 58 FV++EL + V LP D E + +LEE +F S+V+ PLNHS D+ + Sbjct: 13 FVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGDREGCHYDRES 72 Query: 59 GKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALCPLL 118 VTT GFK+A++Q+ EAGW + EFGGQGLP + EMLNS+N ++ + P L Sbjct: 73 KTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLNSSNQAWTMYPGL 132 Query: 119 TDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGTFK 178 + G E L G+++QK+ ++ +SG+WTGTM LTE G+DL L+R++A P+ DG++K Sbjct: 133 SHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSKAEPQADGSYK 192 Query: 179 LFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGSLGARNDVH 238 + G KIFI+ GE+D++ NI+HLVLAR P+APEG KGISLF+VPKFL NADGSLGARN + Sbjct: 193 ITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPNADGSLGARNPIF 252 Query: 239 CVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVS 298 C +IE K+GI + T + GA G L+G+ ++GL+ MF+ MNAAR VG+QGL ++ Sbjct: 253 CGAIEEKMGIHGNATCQMNL---DGATGWLIGQPHKGLQAMFVFMNAARLGVGMQGLGLT 309 Query: 299 ERAYQKAVSYARDRIQSRDLAG-----SPGPVAIIHQPDVRRMLMSMRAQIEAARAL-SY 352 E AYQ A+ YA+DR+Q R L+G P I+H PDVRRML++ +A E RA SY Sbjct: 310 EVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVH-PDVRRMLLTAKAFAEGGRAFTSY 368 Query: 353 VA 354 VA Sbjct: 369 VA 370 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 303 bits (776), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 166/355 (46%), Positives = 217/355 (61%), Gaps = 10/355 (2%) Query: 2 FVMNE-LAGLAAVNALPGCEDATPETAEAILEENARFCSEVIAPLNHSADKAPSSWADGK 60 FV++E L + ALP + + +LEE RF ++V+APLN D+ + +G Sbjct: 14 FVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNGIGDREGCRFKNGT 73 Query: 61 VTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALCPLLTD 120 VTT PGF+ A+ QF GW + E GGQGLP +L EML++AN + + P L Sbjct: 74 VTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAANHGWTMYPGLLH 133 Query: 121 GTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGTFKLF 180 G L G+ + ++ SG+W TM LTE QAGSDL +RTRAVP+ DG+++L Sbjct: 134 GAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTRAVPQDDGSYRLS 193 Query: 181 GTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGSLGARNDVHCV 240 G KIFI+ GEHD+ NIVHLVLAR P+AP G KG+SLF+ P+ L + G+RN VHC Sbjct: 194 GDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDD-----GSRNGVHCT 248 Query: 241 SIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSER 300 IEHK+GI S T L F A LVGE RGL MF+MMNAAR VG QGL ++E Sbjct: 249 GIEHKMGIHGSATCSLHFEQ---AQAWLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEA 305 Query: 301 AYQKAVSYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALSYVAG 355 AYQ+A +YAR+R QSR GS G I+H P V+++LM R +IE AR L+ AG Sbjct: 306 AYQRASAYARERRQSR-APGSSGNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAG 359 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 95.9 bits (237), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 89/290 (30%), Positives = 133/290 (45%), Gaps = 31/290 (10%) Query: 69 EAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLS----FALCPLLTDGTIE 124 EA ++ E G G+ P ++GG G+ + +E + + + + ++ L G E Sbjct: 38 EALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITE 97 Query: 125 ALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGTFKLFGTKI 184 G+ QKE ++ G+ G LTEP GSD A +RTRA +GD F L GTK Sbjct: 98 KY---GSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIRTRAERDGD-DFILNGTKQ 153 Query: 185 FITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGSLGARNDVHCVSIEH 244 FIT G+H V +V A T + G KGIS F+VP + G + R+ E Sbjct: 154 FITSGKH----AGVAIVFAVTDRSA-GKKGISCFLVP---CDTPGFVVGRS-------EE 198 Query: 245 KLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSERAYQK 304 K+G AS T + + L+G+E G + A R + Q + ++ A++ Sbjct: 199 KMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEA 258 Query: 305 AVSYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALSYVA 354 AVSYAR R+ G P II V L M ++AAR + + A Sbjct: 259 AVSYAR----QRESFGVP----IIEHQAVNFRLADMNTLLDAARLMVWRA 300 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 77.0 bits (188), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 90/368 (24%), Positives = 152/368 (41%), Gaps = 46/368 (12%) Query: 16 LPGCEDATPETAEAILEENARFCSEVIAPLNHSADKAPSSWADGKVTTSPGFKEAFRQFG 75 LPG E A+ E A F IAP D++ D +++ G Sbjct: 4 LPGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMD-----------LWKKLG 52 Query: 76 EAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALC-PLLTDGTIEALMTAGTQQQ 134 + G G+ E+GG GL L +E ++ A+ S L ++ + + G ++Q Sbjct: 53 DLGVLGITVSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQ 112 Query: 135 KETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGTFKLFGTKIFITYGEHDMA 194 K ++ ISG + G + ++EP AGSD+ ++ RA +GD + L G+K++IT G Sbjct: 113 KRKYLPRLISGDFIGALAMSEPNAGSDVVSMKLRADKKGD-RYVLNGSKMWITNG----P 167 Query: 195 ENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGSLGARNDVHCVSIEHKLGIKASPTA 254 + V +V A+T + G +G++ F+V K S+ + D KLG++ S T Sbjct: 168 DADVLVVYAKT-DLEAGARGMTAFLVEKGYKGF--SVAQKLD--------KLGMRGSHTG 216 Query: 255 VLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSERAYQKAVSYARDRIQ 314 L F D ++G RG+ + ++ R + L + + V Y DR Q Sbjct: 217 ELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQ 276 Query: 315 SRDLAGSPGPVAIIHQP--DVRRMLMSMRAQIEA-------------ARALSYVAGGVFF 359 + G ++ D+ +M+ +A + A RAL A G Sbjct: 277 ---FGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADSADKVRALRKDAAGAIL 333 Query: 360 XKPSTNTW 367 TW Sbjct: 334 YSAEKATW 341 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,257,227 Number of extensions: 54955 Number of successful extensions: 164 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 8 Length of query: 381 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 294 Effective length of database: 1,207,925 Effective search space: 355129950 Effective search space used: 355129950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)