BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0088.p (319 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 590 e-170 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 238 5e-64 gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 191 7e-50 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 151 9e-38 gi|300309865|ref|YP_003773957.1| D-isomer specific 2-hydroxyacid... 135 3e-33 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 590 bits (1521), Expect = e-170, Method: Compositional matrix adjust. Identities = 289/319 (90%), Positives = 304/319 (95%) Query: 1 MKPHVVVYKKLPEHLLQRLQAECDVTLFDRITQDNRADFLAALKTAHGMIGASLPITNDM 60 MKPHVVVYKKLPEHL+QRLQAE D+TLFDRIT DNRADF+AAL TAHGMIGASLPITN M Sbjct: 1 MKPHVVVYKKLPEHLVQRLQAEFDLTLFDRITDDNRADFIAALATAHGMIGASLPITNAM 60 Query: 61 LEGAPQLRIASTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILSTARRVVE 120 LE APQL+I STISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALIL++ARRVVE Sbjct: 61 LEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARRVVE 120 Query: 121 LAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRPD 180 LAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRPD Sbjct: 121 LAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRPD 180 Query: 181 PQAERELDARYVSQDELLAQADFVCVMLPLNADTERLIGAREFALMKRSAIFINASRGRI 240 P+AEREL ARYVS+++LLAQADFVCVMLPL TER+ GA EFALMKRSAIFINASRGRI Sbjct: 181 PEAERELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRGRI 240 Query: 241 VDEAALIAALQDKTIYGAGLDVFEVEPLPAESPLLQLPNVVALPHIGSATHETRLAMAEL 300 VDEAALIAALQDKTI+GAGLDVFEVEPLP +SPLL+LPNVVALPHIGSATHETRLAMAEL Sbjct: 241 VDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHETRLAMAEL 300 Query: 301 AVCNLLAGLRGEPVPHLAV 319 AV NL+AGLRGEPV HLAV Sbjct: 301 AVTNLIAGLRGEPVAHLAV 319 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 238 bits (607), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 148/325 (45%), Positives = 198/325 (60%), Gaps = 15/325 (4%) Query: 1 MKPHVVVYKKLPEHLLQRLQAECDVTLFDRITQDNRADFLAALKT-AHGMIG----ASLP 55 MKP + V + + +L RL+ +V QD+R A L+ A G G S P Sbjct: 1 MKPKIFVARAVFPEVLARLEQHFEV----ESNQDDRIYSAAELQAKAQGKDGLFTTPSEP 56 Query: 56 ITNDMLEGAPQLRIASTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILSTA 115 +T + PQL+ ++VG +N D+ Q G+M +TP VL E TAD +AL+++ A Sbjct: 57 VTAALFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAA 116 Query: 116 RRVVELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYH 175 RR+ E +++AG+WK ++ G ++HG TLG+IGMGRIG A+ARR+ GF M +LYH Sbjct: 117 RRITESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRS-LGFDMQVLYH 175 Query: 176 NR-RPDPQAE-RELDARYVSQDELLAQADFVCVMLPLNADTERLIGAREFALMKRSAIFI 233 NR R P+ E R ARYVS++ELL QAD V ++LP + DT I A E ALMK +A Sbjct: 176 NRSRLAPELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLT 235 Query: 234 NASRGRIVDEAALIAALQDKTIYGAGLDVFEVEPLPAESP-LLQLPNVVALPHIGSATHE 292 N +RG IVD+AALIAAL++ I AG+DVFE E PA P L L NVV PHI SA+ Sbjct: 236 NIARGGIVDDAALIAALREGRIAAAGVDVFENE--PAFKPEFLDLSNVVLTPHIASASTP 293 Query: 293 TRLAMAELAVCNLLAGLRGEPVPHL 317 TRLAMA A NL+A L G+ P+L Sbjct: 294 TRLAMANCAADNLIAALSGQRPPNL 318 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 191 bits (485), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 130/322 (40%), Positives = 174/322 (54%), Gaps = 20/322 (6%) Query: 2 KPHVVVYKKLPEHLLQRLQAECDVTLFDRITQDNRADFLAALKTAHGMIGAS--LPITND 59 +PHV++ +LP+HLL LQ T + I Q + LAA+ I A+ ++ + Sbjct: 6 RPHVLIVARLPQHLLDLLQQHY--TCHNLILQPHSEAELAAIAPQIRGIAANGEAKVSRE 63 Query: 60 MLEGAPQLRIASTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILSTARRVV 119 + P L I S VG D D+ R+RG+ + HTP VL + AD AL+L+TAR VV Sbjct: 64 FMARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAMALMLATARNVV 123 Query: 120 ELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRP 179 + ++G WK G F V G LG++G+GRIG A+A+RA F M I YHNR Sbjct: 124 RADRFARSGEWKK--GPFPFTTKVSGARLGIVGLGRIGQAIAKRA-AAFDMQISYHNR-- 178 Query: 180 DPQAERELDARYVSQDELLA-QADFVCVMLPLNADTERLIGAREFALMKRSAIFINASRG 238 + +++ YV LA + DF+ ++ P A T L+ A + IN +RG Sbjct: 179 ---SRKDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARG 235 Query: 239 RIVDEAALIAALQDKTIYGAGLDVFEVEP-LPAESPLLQLPNVVALPHIGSATHETRLAM 297 +VDEAALIAAL+ I GAGLDVF EP +PAE L L NVV PH+ S T TR AM Sbjct: 236 SVVDEAALIAALKTGVIAGAGLDVFADEPNVPAE--LAALDNVVLTPHMASGTLVTRTAM 293 Query: 298 AELAVCNLLAGLRG----EPVP 315 A+LA NL A G PVP Sbjct: 294 ADLAFNNLQAHFSGAGVISPVP 315 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 151 bits (381), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 97/253 (38%), Positives = 141/253 (55%), Gaps = 9/253 (3%) Query: 56 ITNDMLEGAPQLRIASTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILSTA 115 ++ +++ AP LR+ S G+D+ D +QRG+ + G A A+ +ALI++ A Sbjct: 60 VSARVMDAAPILRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACA 119 Query: 116 RRVVELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYH 175 + VV L + ++ G W S ++ + + G+TLGL+G+G IG VA A GMP+L H Sbjct: 120 KNVVGLDQRMREGHWDKSTHKS---LELQGRTLGLVGLGAIGRRVAAVAAA-LGMPVLAH 175 Query: 176 NRRPDPQA-ERELDARYVSQDELLAQADFVCVMLPLNADTERLIGAREFALMKRSAIFIN 234 DP A E + V D L AQ+D V + PL A+ + +I A+ A MK AI +N Sbjct: 176 ----DPYAKEAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVN 231 Query: 235 ASRGRIVDEAALIAALQDKTIYGAGLDVFEVEPLPAESPLLQLPNVVALPHIGSATHETR 294 +RG ++DE ALIAAL + AGLD FE EP A PL ++ N V PHIG T + Sbjct: 232 TARGGLIDEQALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVTSDAY 291 Query: 295 LAMAELAVCNLLA 307 +AM A N+LA Sbjct: 292 IAMGTGAASNVLA 304 >gi|300309865|ref|YP_003773957.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 348 Score = 135 bits (341), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 97/269 (36%), Positives = 141/269 (52%), Gaps = 14/269 (5%) Query: 49 MIGASLPITNDMLEGAPQLRIASTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIF 108 + G I+N +++ P L++ + VG D DL++ R +GL + T G LTE AD Sbjct: 81 ITGGHTGISNALMDSLPALKVVAVNGVGTDAVDLNHARSKGLPVTGTFGALTEDVADLAL 140 Query: 109 ALILSTARRVVELAEYVKAGRW--KGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHH 166 L+L+ +R++ +V+ G W S + GK +G++GMG++G A+A+RA Sbjct: 141 GLMLAVSRQICPGHAFVQRGDWVRHPSPTAIPLSRRLSGKRVGIVGMGKVGRAIAQRA-A 199 Query: 167 GFGMPILYHNRRPDPQAERELDARYVSQDEL--LAQADFVCVMLPLNADTERLIGAREFA 224 FG PI Y + R D Y Q EL LA+ V V+ + +I A Sbjct: 200 AFGCPITYTDLRAME------DLPYAFQPELLQLARESDVLVLAAAADKAQGIINAAVLD 253 Query: 225 LMKRSAIFINASRGRIVDEAALIAALQDKTIYGAGLDVFEVEP-LPAESPLLQLPNVVAL 283 + R I IN +RG++V E L+ ALQ I GAGLDVF EP +PA LLQ+ VV Sbjct: 254 ALGREGILINIARGKLVVEEDLVDALQRGVIAGAGLDVFVDEPNVPAA--LLQMDQVVLQ 311 Query: 284 PHIGSATHETRLAMAELAVCNLLAGLRGE 312 H SAT E+R AM E+ + +L GL G+ Sbjct: 312 AHRASATVESRTAMGEMVLASLAQGLAGQ 340 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,021,109 Number of extensions: 44454 Number of successful extensions: 143 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 120 Number of HSP's successfully gapped: 11 Length of query: 319 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 234 Effective length of database: 1,217,395 Effective search space: 284870430 Effective search space used: 284870430 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)