BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0087.p (314 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 582 e-167 gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 104 1e-23 gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspiril... 97 2e-21 gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 66 4e-12 gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum se... 57 2e-09 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 582 bits (1501), Expect = e-167, Method: Compositional matrix adjust. Identities = 288/313 (92%), Positives = 300/313 (95%) Query: 1 MTMQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVG 60 MTMQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVG Sbjct: 1 MTMQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVG 60 Query: 61 NDAFGRFIRKRVAQEGVEVSRVLTDMEFRTAIQLKAKAVDGADPAIEYYRKGSAASHLSL 120 NDAFGRFIR+RVAQEGVEVSRV+TDMEFRTAIQLKAKAV GADPAIEYYRKGSAASHLS+ Sbjct: 61 NDAFGRFIRQRVAQEGVEVSRVITDMEFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSV 120 Query: 121 EDFDPAYFGAARHLHATGIAPALSPTTMAFAHHAMEFMRAQGKTISFDPNLRPMLWPSRE 180 +DFD YFGAARHLHATGIAPALS TTMAFAH AM+FMR QGKTISFDPNLRPMLWPS+E Sbjct: 121 DDFDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLWPSQE 180 Query: 181 VMAQQLNALAFKADWVLPGLSEGTLLTGYEDPHAIAGFYLERGVKMVVIKLGAEGAYWRN 240 VMAQQLNALAFKADWVLPGLSEG +LTG++D IAGFYLERGVK+VVIKLGAEGAYWRN Sbjct: 181 VMAQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIKLGAEGAYWRN 240 Query: 241 SEGEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLLVPEAVMRGNRIGAFAIQVVGDM 300 +GEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGL VP+AVMRGNRIGAFAIQVVGDM Sbjct: 241 GQGEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDM 300 Query: 301 EGLPTRGELEAAS 313 EGLPTR ELEAAS Sbjct: 301 EGLPTRAELEAAS 313 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 104 bits (259), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 101/309 (32%), Positives = 136/309 (44%), Gaps = 8/309 (2%) Query: 3 MQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 M ++ +GEA+ P + ++ Y G +N AI AR G + S VG+D Sbjct: 1 MSKTILAYGEAMVEFNQRIDAPRQFLQGY----GGDTSNFAIAAARQGAASAYLSAVGDD 56 Query: 63 AFGRFIRKRVAQEGVEVSRVLTDMEFRTAIQLKAKAVDGADPAIEYYRKGSAASHLSLED 122 FG+ + +E V+V V T I D A Y R GSAAS + Sbjct: 57 HFGQDLLALWQREEVDVRHVAIAPGGSTGIYFVTH--DAAGHHFHYRRAGSAASAYQAKA 114 Query: 123 FDPAYFGAARHLHATGIAPALSPTTMAFAHHAMEFMRAQGKTISFDPNLRPMLWPSREVM 182 AA LH +GI+ A+S + AM RA G T S D NLR LWP Sbjct: 115 LPLEAIAAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERAR 174 Query: 183 AQQLNALAFKADWVLPGLSEGTLLTGYEDPHAIAGFYLERGVKMVVIKLGAEGAYWRNSE 242 A+ A D LP + +LLTG ED AI L GVK+V KLG EG Y S+ Sbjct: 175 ARMEEAFRL-CDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSD 233 Query: 243 GEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLLVPEAVMRGNRIGAFAIQVVGDMEG 302 VA PV + +D GAGD F ++ ++ G A N A + G +E Sbjct: 234 QRRMVAPYPV-DSLDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEP 292 Query: 303 LPTRGELEA 311 +P R ++EA Sbjct: 293 IPRRAQVEA 301 >gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspirillum seropedicae SmR1] Length = 657 Score = 97.1 bits (240), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 90/335 (26%), Positives = 146/335 (43%), Gaps = 40/335 (11%) Query: 4 QLDVVTWGEALALLVADEVG-PFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 +LD++ G L A +VG P +V + + L G+ N+A G ARLGLK +R+G++ Sbjct: 20 RLDLICIGRLAVDLYAQQVGAPLSDVSSFAKYLGGSSANIAFGCARLGLKSAMLARIGDE 79 Query: 63 AFGRFIRKRVAQEGVEVSRVLTDMEFRTAIQLKAKAVDGADPAIEYYRKGSAASHLSLED 122 A G+F++ +A EG +VS V D + TA+ + + P I +YR A L+ ED Sbjct: 80 ANGQFLKAELAAEGCDVSHVTVDPQRLTALVMLGLKDKDSFPLI-FYRDNCADMALAPED 138 Query: 123 FDPAYFGAARHLHATGIAPALSPTTMAFAHHAMEFMRAQGKTISFDPNLRPMLW------ 176 D A+ +++ L TG + +P + A+++ R D + RP+LW Sbjct: 139 IDEAFIASSKALLITGTHFS-TPQVHRSSSLALQYARRHNVRTVLDIDYRPVLWGLTSKG 197 Query: 177 ------PSREVMAQQLNALAFKADWVLPGLSEGTLLTGYEDPHA---------IAGFYLE 221 + + + Q L + + D V+ E + G D A A ++ Sbjct: 198 DGETRFIASDGVTQHLQGILPQFDLVVGTEEEFMIAGGSTDIMAALRAVRALCAATLVVK 257 Query: 222 RGVKMVVIKLGA------EGAYWRNSEGEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLE 275 RG V+ GA +G ++R GV V EV++ +GAGD F G + G L Sbjct: 258 RGPLGCVVLHGAIPERLDDGFHYR---------GVQV-EVLNVLGAGDAFISGFLKGWLN 307 Query: 276 GLLVPEAVMRGNRIGAFAIQVVGDMEGLPTRGELE 310 N GA + +P EL+ Sbjct: 308 EEDDEHCCRYANACGALVVSRHACAPAMPGPIELD 342 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 65.9 bits (159), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 83/300 (27%), Positives = 113/300 (37%), Gaps = 68/300 (22%) Query: 37 GAETNVAIGLARLGLKVGWASRVGNDAFGRFIRKRVAQEGVEVSRVLTDMEFRTAIQLKA 96 G +N A+ AR+G V + S +G DAFG F + R I Sbjct: 45 GKGSNQAVAAARVGAPVRFISAIGKDAFGDFA---------------LSLWRRETIAPHV 89 Query: 97 KAVDGADP--AIEYYRKGS----------AASHLSLEDFDPAYFGAARHLHATGIAPAL- 143 + +DGA A Y S AAS LS +D + R A A Sbjct: 90 RVIDGAPTGAAFIYVNDASGDNAIIVVPGAASQLSEQDVE-------RERQAIEAAKVFV 142 Query: 144 ----SPTTMAFAHHAMEFMRAQGKTISFDPNLRPMLWPSREVMAQQLNALAFKA------ 193 P A A ++ RA G T F+P ALAF A Sbjct: 143 TQLEQPPEAALA--GLQIARAAGTTTVFNPA----------------PALAFPAQVYGLC 184 Query: 194 DWVLPGLSEGTLLTGYE-----DPHAIAGFYLERGVKMVVIKLGAEGAYWRNSEGEGRVA 248 D++ P E LLTG E D A LERGV +I LGA+G+ + + Sbjct: 185 DFITPNEHEAALLTGIEIHHIDDARRAADVLLERGVGCALITLGAQGSLLHSRSQSLHLP 244 Query: 249 GVPVKEVVDTVGAGDGFAVGVISGMLEGLLVPEAVMRGNRIGAFAIQVVGDMEGLPTRGE 308 + VV+T GAGD F +G+ EG+ EA + A ++ G +P+R E Sbjct: 245 ALCAGTVVETAGAGDAFNGAFAAGLAEGMSAEEAARLATAVAAISVTRPGTAPSMPSRQE 304 >gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum seropedicae SmR1] Length = 299 Score = 57.0 bits (136), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 77/284 (27%), Positives = 120/284 (42%), Gaps = 34/284 (11%) Query: 37 GAETNVAIGLARL---GLKVGWASRVGNDAFGRFIRKRVAQEGVE---VSRVLTDMEFRT 90 G N A+ ARL G +V + VG+DAFG +R+ + G++ + V + Sbjct: 37 GKGANQAVACARLAAPGTRVAMVACVGDDAFGGQMRQSITACGIDDRYIDEVAGEATGIA 96 Query: 91 AIQLKAKAVDGADPAIEYYRKGSAASHLSLEDFDPAYFGAARHLHATGIAPALSPTTMAF 150 +I + A A + A L +E + A + I MA Sbjct: 97 SIMVDANAQN------SIVIAAGANGRLDVERIERARA----LIEQASIVLLQLEVPMAT 146 Query: 151 AHHAMEFMRAQGKTISFDPNLRPMLWPSREVMAQQL-NALAFKADWVLPGLSEGTLLT-- 207 H++E A GKT+ +P P AQ L AL K D+++ E +L Sbjct: 147 VIHSIELAHALGKTVVLNP------AP-----AQALPRALLQKIDYLILNEIEAAMLAEE 195 Query: 208 GYEDPHAIAGFYLERGVKMVVIKLGAEGAYWRNSEGEGRVAGVPVKEV--VDTVGAGDGF 265 ED +A + G + VV+ LG +G Y ++G+ R +P ++V VDT AGD F Sbjct: 196 QSEDIPMLARKLHDLGARNVVVTLGEKGVYGSFADGQQR--HLPARKVQAVDTTAAGDTF 253 Query: 266 AVGVISGMLEGLLVPEAVMRGNRIGAFAIQVVGDMEGLPTRGEL 309 G I + +G EA+ A ++ VG +PTR E+ Sbjct: 254 IGGFIGAIAQGRDQFEAIAYAQAAAALSVTRVGAQTSIPTRDEV 297 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,061,466 Number of extensions: 48685 Number of successful extensions: 172 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 6 Length of query: 314 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 229 Effective length of database: 1,217,395 Effective search space: 278783455 Effective search space used: 278783455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)