BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0070.p (957 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310093|ref|YP_003774185.1| transmembrane protein [Herbaspi... 129 1e-30 gi|300313314|ref|YP_003777406.1| transmembrane protein [Herbaspi... 125 1e-29 >gi|300310093|ref|YP_003774185.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1291 Score = 129 bits (324), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 134/545 (24%), Positives = 228/545 (41%), Gaps = 79/545 (14%) Query: 421 ALKNLEDLDRQLDTLEHHHRNGAPWTTRFGLNHDNALLGALWPHYAIAANQILVTPIRQQ 480 AL+ L+D QLD +R P + GL + L L Y +I++ P+ Sbjct: 494 ALEILQDRIEQLD----RYRASRPISVGMGLYQGDLLERKLREEYFSGIKEIMLKPVASN 549 Query: 481 LEARLLQL--------------ASLSDTEIANSGNE--------------KVQAAYDTLK 512 +E+ L+++ S T +A++G + V+ AY+ LK Sbjct: 550 IESYLMEVNANAARLEPMSRPPQSGGITPVADNGAQAASALRTYKDSSATSVEDAYNALK 609 Query: 513 TYLMLGKPERAAAAFLAPQL-------LATPAPSRPGNATISSGTWEDLRQHTITFFTEH 565 TYLML RA ++ L Q+ L T + P I S + I+F E Sbjct: 610 TYLMLADKSRAESSHLNDQITRFWRGWLETNRGTMPREQMIRSA------ERLISFSLEQ 663 Query: 566 LAAGKLRAAPLDVGLIASARQTIISVRGIQNSTETIYQQILDEADEKYPPLSLATLLGDT 625 + + L+ AR+ + V + E +Y + A ++ +++A ++GD Sbjct: 664 INDPSWPTIDNKLTLVDQARENLRRVVRGMPARERVYADVKARAATRFASMTVARIVGD- 722 Query: 626 SSRGLFNTSSTVPGVFTRAAWDERIAKAIDDASEQRNVSGDWVLS----DASTAQAAPSA 681 R L S +PG FTR AW++ + +A +A+ + S DWVL D T + +P Sbjct: 723 KDRELVMGSYAIPGTFTRDAWEKFVQEAFKEAANKELQSADWVLKTSAKDDLTLEGSPEQ 782 Query: 682 LKTQLRERYFDEYARAWEQFLNSLRWQPASTLSATADQLTLLGDPQRSPLVALMNAIVYQ 741 ++ L +Y EYAR W++FL + L + + LGDP SP+ ++N + + Sbjct: 783 IQKALVTQYKTEYAREWQKFLQGVSIVELRNLDDATNAMNRLGDPITSPMKKVINTVYDE 842 Query: 742 AGTGATAASLSDALISKAQKLVGADEKDPAKQAQPQKPPLSAAFGPILHLTGSDLAASGS 801 T SL DA ++ A+ K+ + P P + A G+ Sbjct: 843 --TSWDNPSLVDAGLANAKSGFMGWFKETILRRSPAPAP-------------TQFNADGT 887 Query: 802 T--PNKP-------ASQVAATGDLS---LARYLERVTAMRLRASQIVSAADPDALARLAA 849 T P P +++ + D + L YL+ ++ +R R + I + D A+ Sbjct: 888 TAIPMGPVGREFSGVARLVVSKDKNGSLLQTYLDNLSKLRTRLNTIKNQGDTGPGAKQLM 947 Query: 850 QSVLQGKTSEISDSRDYA-SRVAASLGEQWAG-FGELLRTPFDQTWQVIVQPAASSLNEI 907 Q L+G SE+SDS + + L +Q LL P Q + +V+P +N+I Sbjct: 948 QQTLEGNGSELSDSLKFVDEEMLPGLNDQQKTVLRPLLVRPLVQGFNALVRPTEGEVNKI 1007 Query: 908 WRTAI 912 WR + Sbjct: 1008 WRAQV 1012 >gi|300313314|ref|YP_003777406.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1266 Score = 125 bits (315), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 133/538 (24%), Positives = 218/538 (40%), Gaps = 63/538 (11%) Query: 421 ALKNLEDLDRQLDTLEHHHRNGAPWTTRFGLNHDNALLGALWPHYAIAANQILVTPIRQQ 480 AL+ L+D QLD H P + GL + L L Y +I++ P+ Sbjct: 494 ALEILQDRIEQLDRYRSHR----PVSIDLGLYQGDLLERKLREEYFAGIKEIMLKPVASS 549 Query: 481 LEARLLQ-------------------LASLSDTEI---------ANSGNEKVQAAYDTLK 512 +E L++ LA+ D I +S V+ AY+ LK Sbjct: 550 IENYLIEVNANSSKLEPMSRPPQADSLATTGDNNIHAGNALRTYKDSSATNVEDAYNALK 609 Query: 513 TYLMLGKPERAAAAFLAPQL-------LATPAPSRPGNATISSGTWEDLRQHTITFFTEH 565 TYLML RA A+ L Q+ L T + P I S + I+F E Sbjct: 610 TYLMLSDKSRAEASHLNDQITRFWRGWLETNRGTMPREQMIRSA------ERLISFSLEQ 663 Query: 566 LAAGKLRAAPLDVGLIASARQTIISVRGIQNSTETIYQQILDEADEKYPPLSLATLLGDT 625 + + L+ R+ + V + E +Y + A ++ +++A ++GD Sbjct: 664 IDDPSWPTTENKLTLVDQTRENLRHVVRGMPARERVYADVKARAATRFASMTVARIVGD- 722 Query: 626 SSRGLFNTSSTVPGVFTRAAWDERIAKAIDDASEQRNVSGDWVLSDAS----TAQAAPSA 681 R L S +PG FTR AW++ + A +A+ + S DWVL +S T + +P Sbjct: 723 KDRELVMGSYAIPGTFTRNAWEKFVEDAFKEAANKELQSADWVLKTSSKEDLTLEGSPEQ 782 Query: 682 LKTQLRERYFDEYARAWEQFLNSLRWQPASTLSATADQLTLLGDPQRSPLVALMNAIVYQ 741 ++ L +Y EYAR W++FL + L + + LGDP SP+ ++ + VY Sbjct: 783 IQKALVSQYKSEYAREWQKFLQGVSIVELHNLDDATNAMNRLGDPLTSPINKVI-STVYD 841 Query: 742 AGTGATAASLSDALISKAQKLVGADEKDPAKQAQPQKPPLSAAFGPILHLTGSDLAASGS 801 + + + L + ++ L G K+ ++ P P L+ G G Sbjct: 842 ETSWDNPSLIGSGLATASRGLSGW-----FKETVLRRSPAPPPAPPQLNTDGGAAIPMGP 896 Query: 802 TPNKPAS----QVAATGDLSLAR-YLERVTAMRLRASQIVSAADPDALARLAAQSVLQGK 856 + A V+ SL R Y+E ++ +R R + I + D A+ Q L G Sbjct: 897 VAREFAGVARLVVSKDKGNSLMRGYMENLSKLRTRLNTIKNQGDTGPGAKQLMQQTLDGS 956 Query: 857 TSEISDSRDYASR--VAASLGEQWAGFGELLRTPFDQTWQVIVQPAASSLNEIWRTAI 912 SE+SD+ + + +Q LL P Q + +V P LN+IWR + Sbjct: 957 GSELSDALKFVDEEMLPGLNDQQKTTLRPLLVRPLVQGFNALVHPTEGELNKIWRAQV 1014 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,764,274 Number of extensions: 107965 Number of successful extensions: 368 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 359 Number of HSP's successfully gapped: 2 Length of query: 957 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 863 Effective length of database: 1,174,780 Effective search space: 1013835140 Effective search space used: 1013835140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)