BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0066.p (497 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313323|ref|YP_003777415.1| hypothetical protein Hsero_4036... 400 e-112 gi|300310077|ref|YP_003774169.1| hypothetical protein Hsero_0742... 398 e-112 >gi|300313323|ref|YP_003777415.1| hypothetical protein Hsero_4036 [Herbaspirillum seropedicae SmR1] Length = 497 Score = 400 bits (1027), Expect = e-112, Method: Compositional matrix adjust. Identities = 192/427 (44%), Positives = 269/427 (62%), Gaps = 2/427 (0%) Query: 71 LDFYIGQLDRQISRQLDAVMHTPEFQALEGRWRGLKMLVSRTDFRKNARIEVLDVSKEAL 130 LD I +LDR IS Q+ A+MH PEFQ LEG W GL LV T +N +I++L+ +K+ L Sbjct: 68 LDARIAELDRLISSQVSAIMHAPEFQKLEGSWTGLNYLVRNTTVGENQKIKILNATKKEL 127 Query: 131 QRDFEDTPELIQSGLYRLTYIEEYDTPGGQPISAMISDFEFTHLPQDIALLRNISKVAAA 190 +DF E QS +++ Y EE+ T GG P A++ DFE T P+D+ + +S++AAA Sbjct: 128 VKDFNSALEFDQSAMFKKVYEEEFGTFGGAPFGALLGDFEITRQPEDMYFIEQMSRIAAA 187 Query: 191 AHMPFIGSVGPAFFGKKSMEEVAAIQDIANYFDRAEYIKWKSFRETDDARYVGLTMPRVL 250 +H PFI + P FG +S E+ +D++ FD EY KWKSFRE++D+RYVGLT+PR L Sbjct: 188 SHAPFITAASPELFGLESFSELGKPRDLSKVFDTVEYAKWKSFRESEDSRYVGLTLPRFL 247 Query: 251 GRLPYG-KDTTPVRAFNYEEAVKGPDHDKYLWMNASFAFAANMVRSFVTNGWCVQIRGPQ 309 GRLP+ KD T V FN+ E V G DH KYLW NA++AF + + ++F GWC IRG + Sbjct: 248 GRLPFNPKDGTTVEGFNFVEDVDGTDHSKYLWCNAAYAFGSKLTKAFEDFGWCAAIRGVE 307 Query: 310 AGGKVDDLPVHLYDLGTG-VQPKIPTEVLIPETREFEFANLGFIPLSFYKNHDFACFFSA 368 GG V+DLP H + G V K PTE+ I + RE E ++LGFI L KN D+A FF A Sbjct: 308 GGGLVEDLPTHTFKTDEGEVALKCPTEIAITDRREKELSDLGFISLVHCKNTDYAAFFGA 367 Query: 369 SSTQKPSLYETKEATANSRINARLPYIFLLSRIAHYLKLIQRENIGTTKDRRLLELELNH 428 S QKP Y + A AN+ ++++L YIF +SRIAHY+K + R+ IG+ +E LN Sbjct: 368 QSAQKPKKYNSDAANANAVLSSQLQYIFAVSRIAHYMKSMMRDKIGSFAAASNVEDFLNR 427 Query: 429 WVKGLVTEMKDPGDELQASHPLREAKVTVEDIEDNPGFFRIKLFIIPHFQVEGMDIGLSL 488 W+ V + E +A PLREA V V ++ PG +R F+ PHFQ++ + + L L Sbjct: 428 WIAQYVLLDDNATQEAKAQFPLREASVQVSEVPGRPGVYRAVSFLRPHFQLDELSVSLRL 487 Query: 489 VSQMPKA 495 V+++P+A Sbjct: 488 VAELPQA 494 >gi|300310077|ref|YP_003774169.1| hypothetical protein Hsero_0742 [Herbaspirillum seropedicae SmR1] Length = 495 Score = 398 bits (1023), Expect = e-112, Method: Compositional matrix adjust. Identities = 195/459 (42%), Positives = 279/459 (60%), Gaps = 2/459 (0%) Query: 39 ASADERVARGMDTLLNLIARESRSVDRLDKSLLDFYIGQLDRQISRQLDAVMHTPEFQAL 98 AS +R + L+N + + V + LD I +LD+ IS Q+ A+MH PEFQ L Sbjct: 34 ASEHDRAKDLITELVNQVMEGTVVVSSNLSATLDARIAELDKLISSQVSAIMHAPEFQKL 93 Query: 99 EGRWRGLKMLVSRTDFRKNARIEVLDVSKEALQRDFEDTPELIQSGLYRLTYIEEYDTPG 158 EG W GL LV T +N +I++L+ +K+ L +DF E QS +++ Y EE+ T G Sbjct: 94 EGSWTGLNYLVRNTTVGENQKIKILNATKKELVKDFNSALEFDQSAMFKKVYEEEFGTFG 153 Query: 159 GQPISAMISDFEFTHLPQDIALLRNISKVAAAAHMPFIGSVGPAFFGKKSMEEVAAIQDI 218 G P ++ DFE T P+D+ + +S++AAA+H PF+ + P FG +S ++ +D+ Sbjct: 154 GAPFGVLLGDFEITRQPEDMYFIEQMSRIAAASHAPFVTAASPELFGLESYADLGKPRDL 213 Query: 219 ANYFDRAEYIKWKSFRETDDARYVGLTMPRVLGRLPYG-KDTTPVRAFNYEEAVKGPDHD 277 + FD EY KWKSFRE++D+RYVGLT+PR LGRLP+ KD T V FN+ E V G DH Sbjct: 214 SKVFDTVEYAKWKSFRESEDSRYVGLTLPRFLGRLPFNPKDGTTVEGFNFVEDVDGSDHS 273 Query: 278 KYLWMNASFAFAANMVRSFVTNGWCVQIRGPQAGGKVDDLPVHLYDLGTG-VQPKIPTEV 336 KYLW NA++AF + ++F GWC IRG + GG V+DLP H + G V K PTE+ Sbjct: 274 KYLWCNAAYAFGTKLTKAFEDFGWCAAIRGVEGGGLVEDLPTHTFKTDEGEVALKCPTEI 333 Query: 337 LIPETREFEFANLGFIPLSFYKNHDFACFFSASSTQKPSLYETKEATANSRINARLPYIF 396 I + RE E ++LGFI L KN D+A FF A S QKP Y T A AN+ ++++L YIF Sbjct: 334 AITDRREKELSDLGFISLVHCKNTDYAAFFGAQSAQKPKKYNTDSANANAVLSSQLQYIF 393 Query: 397 LLSRIAHYLKLIQRENIGTTKDRRLLELELNHWVKGLVTEMKDPGDELQASHPLREAKVT 456 +SRIAHY+K + R+ IG+ +E LN W+ V + E +A PLREA V Sbjct: 394 AVSRIAHYMKSMMRDKIGSFAAASNVEDFLNRWISQYVLLDDNATQEAKAQFPLREASVQ 453 Query: 457 VEDIEDNPGFFRIKLFIIPHFQVEGMDIGLSLVSQMPKA 495 V ++ PG +R F+ PHFQ++ + + L LV+++P+A Sbjct: 454 VSEVPGRPGVYRAVSFLRPHFQLDELSVSLRLVAELPQA 492 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,517,766 Number of extensions: 64500 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 2 Length of query: 497 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 408 Effective length of database: 1,198,455 Effective search space: 488969640 Effective search space used: 488969640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)