BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0062.p (905 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310082|ref|YP_003774174.1| ClpA/B-type chaperone protein [... 712 0.0 gi|300313318|ref|YP_003777410.1| ClpA/B-type chaperone ATPase [H... 692 0.0 gi|300311426|ref|YP_003775518.1| ATP-dependent Clp protease subu... 526 e-150 gi|300310731|ref|YP_003774823.1| ATP-dependent protease ATP-bind... 253 4e-68 >gi|300310082|ref|YP_003774174.1| ClpA/B-type chaperone protein [Herbaspirillum seropedicae SmR1] Length = 893 Score = 712 bits (1838), Expect = 0.0, Method: Compositional matrix adjust. Identities = 418/898 (46%), Positives = 562/898 (62%), Gaps = 32/898 (3%) Query: 9 LLRHLNQHCIATLEAAASLCQGRLTDEITVEHWLLKLIEAGDGDIPAILRHYDIDLNKVW 68 L+ LN E AASLC R E+ +EH L L+E D I+R I Sbjct: 8 LISKLNDSSRLATERAASLCMARGQYEVDLEHLFLALLENPQTDFSLIVRRSGIS----P 63 Query: 69 DALIGGIEHLPRHLRG----KPALSPQLAMILHEAWLSISSKGDGVLIRSGDLLQAIVNS 124 D+L +EH + P LS L + WL S G+ IRSG LL A++ Sbjct: 64 DSLQADLEHEISQFKNGNTRTPVLSSHLQTLFEHGWLIASLDGERPSIRSGHLLLALLTQ 123 Query: 125 PHV--LRGQNTWMLLSVRAAQIERLLPRLGPRSCESHSDIQSKEEPASFAVDQKDEGTCE 182 + L + + + + +++ L +L S E+ + + A+ + +G Sbjct: 124 TELAQLAYRGSKLFARFKVDELKHDLAKLTEGSVEAPALASASASAAADGQADEQQGG-- 181 Query: 183 TLPHKENTVPSAQKVTRNDALQRFTTDLTEKARCGEIDPVFGRDREIQQMVDVLARRRKN 242 + V + R AL ++TT+LT++AR G+IDPV GRD EI+Q VD+L RRR+N Sbjct: 182 ------DPVGEMRAGGRTPALDQYTTNLTQRAREGKIDPVVGRDGEIRQAVDILLRRRQN 235 Query: 243 NPILVGEPGVGKTALVEGLALRVAQQSVPHAIRNVRILTLDLALLQAGAGVKGEFEQRLK 302 NPIL GE GVGKTA+VEGLALR+AQ VP A++ V I TLD+ LLQAGA VKGEFE RLK Sbjct: 236 NPILTGEAGVGKTAVVEGLALRIAQDDVPQALQGVEIHTLDMGLLQAGASVKGEFENRLK 295 Query: 303 NLIDEVQSAPSPILLFIDEAHTLIGAGNTIGGADAANLLKPALARGELRTIAATTWSEYK 362 +IDEV+ +P PI+LFIDEAHT+IGAG G DAANLLKPALARGELRTIAATTWSEYK Sbjct: 296 GVIDEVKKSPHPIILFIDEAHTMIGAGGQAGQNDAANLLKPALARGELRTIAATTWSEYK 355 Query: 363 EFFERDAALERRFQLIKVDEPDDEAACMMLRGLKTRYAEYHNVHISDQALVAAVKLSRRY 422 ++FE+DAAL RRFQ+IKV+EP +E AC MLR + ++ V + D+A+ AV+LS RY Sbjct: 356 KYFEKDAALARRFQVIKVEEPTEEVACAMLRAMAPLMEKHFGVRVYDEAITEAVRLSHRY 415 Query: 423 IPARQLPDKAVDLIDTAAARVRMGLDSPPADLQRAHAAVAALELEQVTLDADERAGIHGR 482 I RQLPDKA+ ++DTA A+V +G ++ PA L+ + L E L+ + R+G Sbjct: 416 ISGRQLPDKAISVLDTACAKVALGQNATPARLEGVTRRLERLAAEIAALERETRSGA-SH 474 Query: 483 QQRRDTLSVELEDARHTLANLQMRYSSELELVRLVHEQRATDSEARDNRDDGT------- 535 R L + A LQ R+ E EL + RA EA Sbjct: 475 ATRLGELQAQHAQAIAEQTELQQRWEQERELNGRIQAARAALEEANQESASSQDSAAARS 534 Query: 536 -LKGIQQKLSEVQGKTPLVFVDVDVAAVARVVAEWTGIPVGNIVENELQSLLRLEQNLAK 594 L+ + +L E QG+TP+V V VD VA +VA WTGIP+G +V++E++++L L+ L + Sbjct: 535 ELQQLLAQLKEHQGETPMVPVQVDGHVVAEIVAGWTGIPLGKMVKDEIKTVLGLDALLKE 594 Query: 595 RVVGQDHALNALAESLRTAKVGLKNEQAPLGVFLLVGPSGVGKTETALALADLLFGGEAT 654 RV+GQ A A+A+ +RT++ L + P GVFL VGPSGVGKTETALALAD+L+GGE Sbjct: 595 RVLGQPQATAAVAQRVRTSRANLDDPGKPKGVFLFVGPSGVGKTETALALADVLYGGERK 654 Query: 655 LTTINMSEYQESHTVSQLKGSPPGYVGYGQGGILTEAVRRRPYSVILLDEVEKAHRDVLE 714 L TINMSEYQE+HTVS LKGSPPGYVGYG+GG+LTEAVRR PYSV+LLDEVEKAH DVLE Sbjct: 655 LITINMSEYQEAHTVSGLKGSPPGYVGYGEGGVLTEAVRRNPYSVVLLDEVEKAHPDVLE 714 Query: 715 MFYQVFDRGMMRDGEGREIDFRNCVILMTSNLASAQIDEATADN--AEI-TQDALLEIIH 771 +F+QVFD+G+M DGEGREIDF+N +I++TSN+AS+Q+ +A + AE+ T D L +I Sbjct: 715 LFFQVFDKGVMDDGEGREIDFKNTIIILTSNVASSQLMQACLNKSAAELPTPDELDVLIR 774 Query: 772 PHLLDHFQPALLARFQSLVYRPLDTDALESIVHLKLAKVAERLLRQHDITMTCDDSLIKE 831 P L+ F+PA L R + + Y P+ + LE I+ LKL ++A R+ H + D +L++ Sbjct: 775 PQLVKAFKPAFLGRLKVIPYYPISDEVLEQIIKLKLGRIANRIAENHKAEFSYDKALVEA 834 Query: 832 MAQLCLARESGARNCDAFLNQSVLPTVSRELLARMASDNMPASIVLSSSMEG--KLTI 887 + C +SGARN D LN S+LP ++ +LARMA + I +S+ +G K TI Sbjct: 835 VLARCTEVDSGARNVDNILNGSLLPEIAESVLARMAEGQGISRIKVSAGKKGDFKYTI 892 >gi|300313318|ref|YP_003777410.1| ClpA/B-type chaperone ATPase [Herbaspirillum seropedicae SmR1] Length = 889 Score = 692 bits (1786), Expect = 0.0, Method: Compositional matrix adjust. Identities = 417/903 (46%), Positives = 567/903 (62%), Gaps = 46/903 (5%) Query: 9 LLRHLNQHCIATLEAAASLCQGRLTDEITVEHWLLKLIEAGDGDIPAILRHYDIDLNKVW 68 L+ LN+ E AA+LC E+ +EH L L+E D I R ID Sbjct: 8 LICKLNETARLATERAATLCVSCSHYEVDLEHLFLALLEQPQSDFSLIARRSGIDT---- 63 Query: 69 DALIGGIEHLPRHLRG----KPALSPQLAMILHEAWLSISSKGDGVLIRSGDLLQAIVNS 124 D L +EH R P LSP L + WL S + +RS LL A++ Sbjct: 64 DGLQADLEHEISTFREGNARTPVLSPHLRTLFENGWLIASLEESNPSVRSAHLLLALLTQ 123 Query: 125 PHV--LRGQNTWMLLSVRAAQIERLLPRLGPRSCESHSDIQSKEEPASFAVDQKDEGTCE 182 + L + + + + +I+ L RL S ES + ++ + D++ G Sbjct: 124 SDLSQLAYRGSKLFAKFKIDEIKHNLHRLTEGSVESAAIGKTGQ-----GGDRESGGD-- 176 Query: 183 TLPHKENTVPSAQKVTRNDALQRFTTDLTEKARCGEIDPVFGRDREIQQMVDVLARRRKN 242 P E K+T AL ++TT LT++AR G+IDPV GRD EI+Q VD+L RRR+N Sbjct: 177 --PMGELKQLRIGKMT---ALDQYTTQLTQRARDGKIDPVIGRDAEIRQAVDILLRRRQN 231 Query: 243 NPILVGEPGVGKTALVEGLALRVAQQSVPHAIRNVRILTLDLALLQAGAGVKGEFEQRLK 302 NPIL GE GVGKTA+VEGLALR+AQ VP ++ V I TLD+ LLQAGA VKGEFE RLK Sbjct: 232 NPILTGEAGVGKTAVVEGLALRIAQGDVPKPLQGVEIHTLDMGLLQAGASVKGEFESRLK 291 Query: 303 NLIDEVQSAPSPILLFIDEAHTLIGAGNTIGGADAANLLKPALARGELRTIAATTWSEYK 362 +IDEV+ + PI+LFIDEAHT+IGA G +DAANLLKPALARGELRTIAATTWSEYK Sbjct: 292 TVIDEVKKSAYPIILFIDEAHTMIGAAGQAGQSDAANLLKPALARGELRTIAATTWSEYK 351 Query: 363 EFFERDAALERRFQLIKVDEPDDEAACMMLRGLKTRYAEYHNVHISDQALVAAVKLSRRY 422 ++FE+DAAL RRFQ+IKV+EP ++ AC MLR + ++ V + D+A+V AV+LS RY Sbjct: 352 KYFEKDAALARRFQVIKVEEPGEDVACAMLRAMVPLMEQHFGVRVYDEAVVEAVRLSHRY 411 Query: 423 IPARQLPDKAVDLIDTAAARVRMGLDSPPADLQRAHAAVAALELEQVTLDADERAGIHGR 482 I RQLPDKA+ ++DTA A+V +G D+ PA L+ + L+ E L + +G Sbjct: 412 ISGRQLPDKAISVLDTACAKVALGRDATPALLEGVTRRLERLDTEISALRREAASG---- 467 Query: 483 QQRRDTLSVELEDARHTLA-----NLQMRYSSELELVRLVHEQRAT-----DSEARDNRD 532 Q + L+ L+ +H A +LQ+R+ E EL + + RAT +AR Sbjct: 468 SQHEERLASLLD--QHVCAVAERHDLQLRWEQERELNERIQQARATLENPGTGDARGGNV 525 Query: 533 DGTLKGIQQKLSEV---QGKTPLVFVDVDVAAVARVVAEWTGIPVGNIVENELQSLLRLE 589 D ++Q L E+ QG P+V + VD VA +VA WTGIP+G +V++E+Q++L L+ Sbjct: 526 DAARAELKQLLDELRQRQGDAPMVPIQVDGHVVAEIVAAWTGIPLGKMVKDEIQTVLDLD 585 Query: 590 QNLAKRVVGQDHALNALAESLRTAKVGLKNEQAPLGVFLLVGPSGVGKTETALALADLLF 649 L +RV+GQ A +A+A+ +RT++ L + P GVFL VGPSGVGKTETALALAD+L+ Sbjct: 586 ALLQERVLGQPQATSAVAQRVRTSRANLDDPGKPKGVFLFVGPSGVGKTETALALADVLY 645 Query: 650 GGEATLTTINMSEYQESHTVSQLKGSPPGYVGYGQGGILTEAVRRRPYSVILLDEVEKAH 709 GGE L TINMSEYQE+HTVS LKGSPPGYVGYG+GG+LTEAVRR PYSV+LLDEVEKAH Sbjct: 646 GGERKLITINMSEYQEAHTVSGLKGSPPGYVGYGEGGVLTEAVRRNPYSVVLLDEVEKAH 705 Query: 710 RDVLEMFYQVFDRGMMRDGEGREIDFRNCVILMTSNLASAQIDEATADNAE---ITQDAL 766 DVLE+F+QVFD+G+M D EGREIDF+N +I++TSN+ASAQ+ +A + ++ T + L Sbjct: 706 ADVLELFFQVFDKGVMDDAEGREIDFKNTIIILTSNVASAQMMQACLNKSQEELPTPEEL 765 Query: 767 LEIIHPHLLDHFQPALLARFQSLVYRPLDTDALESIVHLKLAKVAERLLRQHDITMTCDD 826 +I P L+ F+PA L R + + Y P+ L I+ LKLA++A+R+ H + + D Sbjct: 766 DTLIRPQLIKTFKPAFLGRLKVVPYYPIADVVLVEIIKLKLARIAKRVAENHRVEFSYDK 825 Query: 827 SLIKEMAQLCLARESGARNCDAFLNQSVLPTVSRELLARMASDNMPASIVLSSSMEG--K 884 +L++ + C +SGARN D L+ ++LP V+ +L RMA I +S+S +G K Sbjct: 826 ALVEAVLGRCTEVDSGARNVDNILSGTLLPAVAESVLVRMAEGVEIRRIKVSASKKGDFK 885 Query: 885 LTI 887 TI Sbjct: 886 YTI 888 >gi|300311426|ref|YP_003775518.1| ATP-dependent Clp protease subunit [Herbaspirillum seropedicae SmR1] Length = 861 Score = 526 bits (1354), Expect = e-150, Method: Compositional matrix adjust. Identities = 292/699 (41%), Positives = 433/699 (61%), Gaps = 31/699 (4%) Query: 190 TVPSAQKVTRNDALQRFTTDLTEKARCGEIDPVFGRDREIQQMVDVLARRRKNNPILVGE 249 +V S Q + +AL+++T DLTE+AR G++DPV GRD EI++ + VL RR KNNP+L+GE Sbjct: 148 SVSSQQDEGQREALKKYTLDLTERARAGKLDPVIGRDDEIRRAIQVLQRRSKNNPVLIGE 207 Query: 250 PGVGKTALVEGLALRVAQQSVPHAIRNVRILTLDLALLQAGAGVKGEFEQRLKNLIDEVQ 309 PGVGKTA+VEGLA R+ VP ++++ R+L+LD+A L AGA +GEFE+RLK ++ E+ Sbjct: 208 PGVGKTAIVEGLAQRIVNGEVPDSLKSKRVLSLDMAALLAGAKYRGEFEERLKAVLKEIA 267 Query: 310 SAPSPILLFIDEAHTLIGAGNTIGGADAANLLKPALARGELRTIAATTWSEYKEFFERDA 369 ++FIDE HT++GAG G DA N+LKPALARGEL + ATT EY+++ E+DA Sbjct: 268 QDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDA 327 Query: 370 ALERRFQLIKVDEPDDEAACMMLRGLKTRYAEYHNVHISDQALVAAVKLSRRYIPARQLP 429 ALERRFQ I VDEP EA +LRGL+ +Y +H V I+D A+VAA +LS RYI R LP Sbjct: 328 ALERRFQKILVDEPSVEATIAILRGLQEKYEVHHGVDITDPAIVAAAELSHRYITDRFLP 387 Query: 430 DKAVDLIDTAAARVRMGLDSPPADLQRAHAAVAALELEQVTLDADERAGIHGRQQRRDTL 489 DKA+DLID AA+++++ +DS P + + + L++E+ + ++ R Q + Sbjct: 388 DKAIDLIDEAASKIKIEIDSKPEVMDKLDRRLIQLKIEREAVKREKDEASQKRLQLIEEE 447 Query: 490 SV----------ELEDARHTLANLQMRYSSELELVRLVHEQ--RATDSEARDNRDDGTLK 537 E+ A + A + E+E VRL E+ R +D + G L Sbjct: 448 IEKLEREYADLEEIWKAEKSSAQGGQQLKEEIEKVRLQMEEATRKSDWQKVSELKYGKLA 507 Query: 538 GIQQKLSEVQGKT----------PLVFVDVDVAAVARVVAEWTGIPVGNIVENELQSLLR 587 ++ L EVQ K LV V +A +VA TGIPV +++ E + LL Sbjct: 508 ELEAAL-EVQNKKDAAGVKTEKPKLVRTQVGAEEIAEIVARATGIPVSRMMQGEREKLLH 566 Query: 588 LEQNLAKRVVGQDHALNALAESLRTAKVGLKNEQAPLGVFLLVGPSGVGKTETALALADL 647 +E L +RVVGQD A+ A+++++R ++ GL + P G F+ +GP+GVGKTE ALA Sbjct: 567 MEDVLHERVVGQDEAIVAVSDAIRRSRAGLGDPSKPYGSFMFLGPTGVGKTELCKALASY 626 Query: 648 LFGGEATLTTINMSEYQESHTVSQLKGSPPGYVGYGQGGILTEAVRRRPYSVILLDEVEK 707 LF E + I+MSE+ E H+V++L G+PPGYVGY +GG LTEAVRR+PYSVILLDE+EK Sbjct: 627 LFDTEEAMIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEK 686 Query: 708 AHRDVLEMFYQVFDRGMMRDGEGREIDFRNCVILMTSNLASAQIDEATADNAEITQDALL 767 AH DV + QV D G M DG+GR +DF+N VI+MTSNL S +I + E + AL+ Sbjct: 687 AHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSHKI-----QSMEDSDPALV 741 Query: 768 EI-IHPHLLDHFQPALLARFQSL-VYRPLDTDALESIVHLKLAKVAERLLRQHDITMTCD 825 ++ + + HF+P + R + V+ LD + +I ++L ++ E+ L + +I + Sbjct: 742 KLAVMAEVRTHFRPEFINRVDEIVVFHALDAKNIGAIAKIQL-RILEQRLAKLEIGLEIS 800 Query: 826 DSLIKEMAQLCLARESGARNCDAFLNQSVLPTVSRELLA 864 ++ ++++A+ GAR + Q + +S+++LA Sbjct: 801 EAGLQKIAEAGFDPVYGARPLKRAIQQEIENPLSKQILA 839 >gi|300310731|ref|YP_003774823.1| ATP-dependent protease ATP-binding specificity subunit [Herbaspirillum seropedicae SmR1] Length = 767 Score = 253 bits (647), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 125/253 (49%), Positives = 178/253 (70%), Gaps = 2/253 (0%) Query: 194 AQKVTRNDALQRFTTDLTEKARCGEIDPVFGRDREIQQMVDVLARRRKNNPILVGEPGVG 253 A+ + L +FT +L + A G+IDP+ GR+ E+++++ L RRRKNNP+LVGE GVG Sbjct: 163 AEGQQKESPLDQFTQNLNKAAAEGKIDPLIGRESEVERVIQTLCRRRKNNPLLVGEAGVG 222 Query: 254 KTALVEGLALRVAQQSVPHAIRNVRILTLDLALLQAGAGVKGEFEQRLKNLIDEVQSAPS 313 KTA+ EGLA RV Q VP ++N + +LD+ L AG +G+FEQRLK ++ +++ +P+ Sbjct: 223 KTAIAEGLAWRVTQGDVPEILKNAVVYSLDMGALLAGTKYRGDFEQRLKAVLKQLKDSPN 282 Query: 314 PILLFIDEAHTLIGAGNTIGGA-DAANLLKPALARGELRTIAATTWSEYKEFFERDAALE 372 I LFIDE HT+IGAG+ GG DA+NLLKPAL+ G+L+ I ATT++E++ FE+D AL Sbjct: 283 GI-LFIDEIHTIIGAGSASGGTLDASNLLKPALSSGQLKCIGATTYTEFRGVFEKDHALS 341 Query: 373 RRFQLIKVDEPDDEAACMMLRGLKTRYAEYHNVHISDQALVAAVKLSRRYIPARQLPDKA 432 RRFQ I V+EP E +LRGLK+++ E+H V S AL +A +L+ R+I R LPDKA Sbjct: 342 RRFQKIDVNEPTVEQTVQILRGLKSKFEEHHGVKYSASALTSAAELAARFINDRHLPDKA 401 Query: 433 VDLIDTAAARVRM 445 +D+ID A A R+ Sbjct: 402 IDVIDEAGAAQRI 414 Score = 188 bits (478), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 110/335 (32%), Positives = 187/335 (55%), Gaps = 24/335 (7%) Query: 562 VARVVAEWTGIPVGNIVENELQSLLRLEQNLAKRVVGQDHALNALAESLRTAKVGLKNEQ 621 + ++++ IP ++ +++ L ++++L V GQD A+ AL+ +++ A+ GL Sbjct: 429 IEEIISKIARIPPQSVNQDDRAKLQTIDRDLKNVVFGQDPAIEALSSAIKMARAGLGKTD 488 Query: 622 APLGVFLLVGPSGVGKTETALALADLLFGGEATLTTINMSEYQESHTVSQLKGSPPGYVG 681 P+G FL GP+GVGKTE A LA + G E L +MSEY E H VS+L G+PPGYVG Sbjct: 489 KPIGSFLFSGPTGVGKTEVAKQLA-FILGIE--LVRFDMSEYMERHAVSRLIGAPPGYVG 545 Query: 682 YGQGGILTEAVRRRPYSVILLDEVEKAHRDVLEMFYQVFDRGMMRDGEGREIDFRNCVIL 741 + QGG+LTEAV ++P++V+LLDE+EKAH D+ + QV D G + D GR+ DFRN +I+ Sbjct: 546 FDQGGLLTEAVNKKPHAVLLLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKTDFRNVIII 605 Query: 742 MTSNLASAQIDEATADNAEITQ--DALLEIIHPHLLDHFQPALLARFQSLV-YRPLDTDA 798 MT+N + + + T E + D + +I F P R +++ +RPLD + Sbjct: 606 MTTNAGAESLQKRTIGFTEKKEAGDEMADIKR-----MFTPEFRNRLDAIISFRPLDEEI 660 Query: 799 LESIVHLKLAKVAERLLRQHDITMTCDDSLIKEMAQLCLARESGARNCDAFLNQSVLPTV 858 + +V L ++ E+ L + + DSL K + G + D + + + Sbjct: 661 ILRVVDKFLMQLEEQ-LHEKKVEAVFSDSLRKFL---------GKKGFDPLMGARPMSRL 710 Query: 859 SRELLARMASDNMPASIVLSSSMEGKLTIDFIDRD 893 ++L+ + +D + ++S G++T++ D+D Sbjct: 711 IQDLIRKALADELLFGKLVSG---GRVTVEMDDKD 742 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,733,188 Number of extensions: 116948 Number of successful extensions: 564 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 546 Number of HSP's successfully gapped: 5 Length of query: 905 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 811 Effective length of database: 1,174,780 Effective search space: 952746580 Effective search space used: 952746580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)