BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0058.p (265 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310818|ref|YP_003774910.1| nitrate/sulfonate/bicarbonate A... 493 e-141 gi|300309717|ref|YP_003773809.1| nitrate/sulfonate/bicarbonate A... 220 9e-59 gi|300312685|ref|YP_003776777.1| nitrate/sulfonate/bicarbonate A... 189 3e-49 gi|300311841|ref|YP_003775933.1| nitrate/sulfonate/bicarbonate A... 187 6e-49 gi|300310542|ref|YP_003774634.1| nitrate transporter ATPase [Her... 187 1e-48 >gi|300310818|ref|YP_003774910.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 265 Score = 493 bits (1269), Expect = e-141, Method: Compositional matrix adjust. Identities = 249/265 (93%), Positives = 259/265 (97%) Query: 1 MGHGSSVSIKRVGLTIGKNEIMAGIDLDIRAGEFICLLGPSGCGKSTLLNAIAGFQDVAG 60 M HGSSVS+KRVGLTIG N+IMAGIDLDIRAGEF+CLLGPSGCGKSTLLNAIAGFQDV+G Sbjct: 1 MAHGSSVSLKRVGLTIGSNDIMAGIDLDIRAGEFVCLLGPSGCGKSTLLNAIAGFQDVSG 60 Query: 61 EISVAGKRVKGPGLDRGVVFQSSEALFPWLSVRENVEYGLKLRGIPAAERRSAAERYVSL 120 EISV GKRVKGPGLDRGVVFQSSEALFPWLSVRENVEYGLKLRG+ +AERR+AAERYVSL Sbjct: 61 EISVDGKRVKGPGLDRGVVFQSSEALFPWLSVRENVEYGLKLRGVASAERRAAAERYVSL 120 Query: 121 VGLKHAIDKFPAELSGGMRQRAQIARVLVNEPSVVLMDEPFGALDAQTREVLQSELDRIW 180 VGLKHAIDKFPAELSGGMRQRAQIARVLVNEPSVVLMDEPFGALDAQTREVLQSELDRIW Sbjct: 121 VGLKHAIDKFPAELSGGMRQRAQIARVLVNEPSVVLMDEPFGALDAQTREVLQSELDRIW 180 Query: 181 KSTGCTIVFVTHDIWEAILLADRVVTMTAGPRAHIKAIEEVDLPHPRDPADPACMALYAR 240 KSTGCTIVFVTHDIWEAILLADRVVTMTAGPRAHIK IEEV LPHPRDPADPACMALYAR Sbjct: 181 KSTGCTIVFVTHDIWEAILLADRVVTMTAGPRAHIKTIEEVHLPHPRDPADPACMALYAR 240 Query: 241 IRDDIGAEVTRVLRAQGLIEEESIQ 265 IRDDIGAEVTRVLRAQGL+EEES++ Sbjct: 241 IRDDIGAEVTRVLRAQGLLEEESVK 265 >gi|300309717|ref|YP_003773809.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 220 bits (560), Expect = 9e-59, Method: Compositional matrix adjust. Identities = 120/217 (55%), Positives = 145/217 (66%), Gaps = 3/217 (1%) Query: 22 MAGIDLDIRAGEFICLLGPSGCGKSTLLNAIAGFQ-DVAGEISVAGKRVKGPGLDRGVVF 80 + I+LDI G+F+CLLGPSGCGKSTLLNAIAGF +G+I K V PG DRG+VF Sbjct: 29 LKDINLDIPDGQFVCLLGPSGCGKSTLLNAIAGFALPSSGQILTDDKVVSEPGPDRGMVF 88 Query: 81 QSSEALFPWLSVRENVEYGLKLRGIPAAERRSAAERYVSLVGLKHAIDKFPAELSGGMRQ 140 Q ALFPW++V +NV +GL+++G P AE + + +GL +FP +LSGGMRQ Sbjct: 89 QEY-ALFPWMTVEQNVAFGLEIKGTPKAEIHQRVTQLLDKLGLIDFRTRFPKDLSGGMRQ 147 Query: 141 RAQIARVLVNEPSVVLMDEPFGALDAQTREVLQSELDRIWKSTGCTIVFVTHDIWEAILL 200 R IARVL + ++LMDEPFGALDA TR LQ EL RIW TIVFVTH I EAI L Sbjct: 148 RVAIARVLALDSPIMLMDEPFGALDALTRRNLQDELLRIWDEFRKTIVFVTHSIEEAIYL 207 Query: 201 ADRVVTMTAGPRAHIKAIEEVDLPHPRDPADPACMAL 237 ADR+V MT P +K V+LP RDPADP AL Sbjct: 208 ADRIVVMTYRP-GTVKRDMLVNLPRLRDPADPEFNAL 243 >gi|300312685|ref|YP_003776777.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 275 Score = 189 bits (479), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 95/204 (46%), Positives = 134/204 (65%), Gaps = 3/204 (1%) Query: 25 IDLDIRAGEFICLLGPSGCGKSTLLNAIAGF-QDVAGEISVAGKRVKGPGLDRGVVFQSS 83 + + GEF+ ++GP+GCGKST L+ I G + GE+ V G+ V G G VFQ+ Sbjct: 45 FSMSVARGEFVAVVGPTGCGKSTTLSLITGLLKPTLGEVRVMGQPVNGIDPRIGFVFQN- 103 Query: 84 EALFPWLSVRENVEYGLKLRGIPAAERRSAAERYVSLVGLKHAIDKFPAELSGGMRQRAQ 143 +A+FPW SVRENV G RG P A+ + A+ ++ VGL D +P +LSGGMR+R Sbjct: 104 DAVFPWRSVRENVAAGPLFRGQPKAQAHALADEWIRRVGLDKFGDHYPHQLSGGMRKRVA 163 Query: 144 IARVLVNEPSVVLMDEPFGALDAQTREVLQSELDRIWKSTGCTIVFVTHDIWEAILLADR 203 +A+ +N P ++LMDEPF ALD QTR ++Q EL R+W + ++VFVTHD+ EAI LAD+ Sbjct: 164 LAQTFINSPEILLMDEPFSALDMQTRTLMQDELLRLWSAHSGSVVFVTHDLEEAIALADK 223 Query: 204 VVTMTAGPRAHIKAIEEVDLPHPR 227 V +TA P A +K++ +DLP PR Sbjct: 224 VYVLTARP-ATLKSVYPIDLPRPR 246 >gi|300311841|ref|YP_003775933.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 187 bits (476), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 105/229 (45%), Positives = 139/229 (60%), Gaps = 15/229 (6%) Query: 7 VSIKRVGLTIGKNEIMA----GIDLDIRAGEFICLLGPSGCGKSTLLNAIAGF-QDVAGE 61 + I RV L + + + ++ G+ LLGPSGCGKSTLL AI G+ Q +G Sbjct: 18 LEIDRVTLQYKTTDSLVTATQSVSFNVHPGDRYILLGPSGCGKSTLLKAIGGYLQPTSGA 77 Query: 62 ISVAGKRVKGPGLDRGVVFQSSEALFPWLSVRENVEYGL----KLRGIPAAERRSAAERY 117 I + G+ V PG DR +VFQ + L PW +VR+NVE+ L +L+G AAER AE Y Sbjct: 78 IRLKGREVHEPGPDRMMVFQEFDQLLPWKTVRQNVEFALHASGRLQGRAAAER---AEYY 134 Query: 118 VSLVGLKHAIDKFPAELSGGMRQRAQIARVLVNEPSVVLMDEPFGALDAQTREVLQSELD 177 +S VGL ID +P LSGGM+QR IAR + EP V+LMDEPF ALDA TR +Q EL Sbjct: 135 ISKVGLAKFIDSYPHMLSGGMKQRVSIARGMAMEPDVLLMDEPFAALDALTRRKMQDELL 194 Query: 178 RIWKSTGCTIVFVTHDIWEAILLADRVVTMTAGP---RAHIKAIEEVDL 223 R+W T T++FVTH I EAI + R++ +T P RA + +I+ L Sbjct: 195 RLWDDTQFTVLFVTHSIEEAIRVGTRILLLTPHPGQVRAELNSIDPSQL 243 >gi|300310542|ref|YP_003774634.1| nitrate transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 272 Score = 187 bits (474), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 106/209 (50%), Positives = 134/209 (64%), Gaps = 7/209 (3%) Query: 25 IDLDIRAGEFICLLGPSGCGKSTLLNAIAGF-QDVAGEISVAGKRVKGPGLDRGVVFQSS 83 I+L + GEF+ L+G SGCGKSTLLN IAG + G + A + + GP +R VVFQ+ Sbjct: 33 INLTVAKGEFVTLIGHSGCGKSTLLNLIAGLLKPSEGVLLCANREIGGPAPERAVVFQN- 91 Query: 84 EALFPWLSVRENVEYGLKLRGIPAAERRSA----AERYVSLVGLKHAIDKFPAELSGGMR 139 +L PWLS EN+ G++ R A E R+ ++LVGL A +K P E+SGGM+ Sbjct: 92 HSLLPWLSCYENIYLGVE-RVFGATENRTQLRDRTRAALALVGLSAAENKRPNEISGGMK 150 Query: 140 QRAQIARVLVNEPSVVLMDEPFGALDAQTREVLQSELDRIWKSTGCTIVFVTHDIWEAIL 199 QR IAR L EP V+LMDEPFGALDA TR LQ EL +I T T+V VTHD+ EA+L Sbjct: 151 QRVGIARALAMEPKVLLMDEPFGALDALTRAHLQDELLKIVAKTDSTVVMVTHDVDEAVL 210 Query: 200 LADRVVTMTAGPRAHIKAIEEVDLPHPRD 228 L+DR+V MT GP A I + EV L PRD Sbjct: 211 LSDRIVMMTNGPAATIGEVLEVKLARPRD 239 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 854,942 Number of extensions: 36385 Number of successful extensions: 511 Number of sequences better than 1.0e-08: 129 Number of HSP's gapped: 329 Number of HSP's successfully gapped: 145 Length of query: 265 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 181 Effective length of database: 1,222,130 Effective search space: 221205530 Effective search space used: 221205530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)