BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0057.p (256 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate A... 481 e-137 gi|300311644|ref|YP_003775736.1| taurine ABC transporter permeas... 129 2e-31 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 107 1e-24 gi|300310541|ref|YP_003774633.1| nitrate transporter permease [H... 101 6e-23 gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 98 5e-22 >gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 256 Score = 481 bits (1237), Expect = e-137, Method: Compositional matrix adjust. Identities = 246/256 (96%), Positives = 254/256 (99%) Query: 1 MPSSLGRRMKAARPFLISVVAIVGGLVLWQLVSMTTSPLFLPSVSMTWAAALELISDGTL 60 MPS+LGRR+KAARPFLISV+AIVGGLVLWQLVS+TTSPLFLPSVSMTWAAALELISDGTL Sbjct: 1 MPSNLGRRLKAARPFLISVIAIVGGLVLWQLVSLTTSPLFLPSVSMTWAAALELISDGTL 60 Query: 61 QQSVLASSGRILAGWGAGVAIGVPLGIFMGRFDLVRQLLDPYIEFFRFIPPIAFVTLAVI 120 QQSVLASSGRILAGWGAGV IGVPLGIFMGRF+ VRQLLDPYIEFFRFIPPIAFVTLAVI Sbjct: 61 QQSVLASSGRILAGWGAGVVIGVPLGIFMGRFETVRQLLDPYIEFFRFIPPIAFVTLAVI 120 Query: 121 WLGPGELSKIALIFYTTVFIVTLNTIAGVQAVNPLRLRAAASLGAGQWKILTTVILPSTV 180 WLGPGELSKIALIFYTTVFIVTLNTIAGVQAVNPLRLRAAASLGAGQWKIL+TVILPSTV Sbjct: 121 WLGPGELSKIALIFYTTVFIVTLNTIAGVQAVNPLRLRAAASLGAGQWKILSTVILPSTV 180 Query: 181 PFMVTGARIAMGNSFLTVVSAEIVSAREGLGALIWTARNFSRTEWVFVGIIALGLLGYLF 240 PFMVTGARIAMGNSFLTVVSAEIVSAREGLGALIWTARNFSRTEWVFVGIIALG+LGYLF Sbjct: 181 PFMVTGARIAMGNSFLTVVSAEIVSAREGLGALIWTARNFSRTEWVFVGIIALGILGYLF 240 Query: 241 DRVLRIATRKLLARYL 256 DRVLR+ATRKLLARYL Sbjct: 241 DRVLRVATRKLLARYL 256 >gi|300311644|ref|YP_003775736.1| taurine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 129 bits (324), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 94/242 (38%), Positives = 145/242 (59%), Gaps = 6/242 (2%) Query: 17 ISVVAIVGGLVLWQLVSMT--TSPLFLPSVSMTWAAALELISDG----TLQQSVLASSGR 70 IS+ A + L LW L++ T LFLP+ + A + DG TL + V AS R Sbjct: 46 ISIAAGIVILALWWLIAQTGWVPHLFLPTPAEVLKVAQSIWEDGYANATLAEHVGASLLR 105 Query: 71 ILAGWGAGVAIGVPLGIFMGRFDLVRQLLDPYIEFFRFIPPIAFVTLAVIWLGPGELSKI 130 IL V +G+P+G+ MG V+ +LD IEF+ +PP+A++ L +IWLG GE SKI Sbjct: 106 ILTAGVIAVLLGIPVGLLMGLNRWVKGVLDTPIEFYWPLPPLAYLPLMIIWLGIGEASKI 165 Query: 131 ALIFYTTVFIVTLNTIAGVQAVNPLRLRAAASLGAGQWKILTTVILPSTVPFMVTGARIA 190 L+ V L+ AGV+++ R+ AA SLGA +W++LT V+LPS +P ++TG RIA Sbjct: 166 TLLTLAMFAPVVLSAQAGVRSLPQERVNAALSLGATRWQLLTEVVLPSALPEILTGIRIA 225 Query: 191 MGNSFLTVVSAEIVSAREGLGALIWTARNFSRTEWVFVGIIALGLLGYLFDRVLRIATRK 250 +G + T+V+AE++++ G+G +I +A +F T+ VFVGI + + F +R+ R Sbjct: 226 LGVGWSTLVAAELIASNRGIGYMIMSASHFLATDAVFVGIALIATCAFAFSYGMRLLERA 285 Query: 251 LL 252 L+ Sbjct: 286 LV 287 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 107 bits (266), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 57/144 (39%), Positives = 91/144 (63%) Query: 98 LLDPYIEFFRFIPPIAFVTLAVIWLGPGELSKIALIFYTTVFIVTLNTIAGVQAVNPLRL 157 L++ ++ R IPPIA++ LA++W G G + LI F V +NTIAGV+ V+ + L Sbjct: 111 LMNLTVQVIRPIPPIAYIPLAILWFGLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYL 170 Query: 158 RAAASLGAGQWKILTTVILPSTVPFMVTGARIAMGNSFLTVVSAEIVSAREGLGALIWTA 217 RAA +LGA Q + V+LP+ VP++++G RI +G +F+ V+ AE+++ GLG I A Sbjct: 171 RAARNLGASQSTLFLRVMLPAAVPYILSGVRIGIGTAFIVVIVAEMIAVSNGLGFRIMEA 230 Query: 218 RNFSRTEWVFVGIIALGLLGYLFD 241 R + ++ + G+I +GLLG D Sbjct: 231 REYFWSDKIIAGMITIGLLGLAID 254 >gi|300310541|ref|YP_003774633.1| nitrate transporter permease [Herbaspirillum seropedicae SmR1] Length = 307 Score = 101 bits (252), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 57/198 (28%), Positives = 109/198 (55%), Gaps = 7/198 (3%) Query: 29 WQLVSMTTSPLFLPSVSMTWAAAL-------ELISDGTLQQSVLASSGRILAGWGAGVAI 81 WQ++++ TS P V++ A + +D + +VLAS R+ G+G + Sbjct: 71 WQIIALKTSSFPTPWVTLKEAGVMFADPFYRNSPNDQGIGWNVLASLQRVGTGFGLAALV 130 Query: 82 GVPLGIFMGRFDLVRQLLDPYIEFFRFIPPIAFVTLAVIWLGPGELSKIALIFYTTVFIV 141 G+PLG +GR + + ++ P I R + P+A++ + ++ + I IF +++ + Sbjct: 131 GIPLGFAIGRVEFLARMFGPIISLLRPVSPLAWLPIGLLVFKSANPAAIWSIFICSIWPM 190 Query: 142 TLNTIAGVQAVNPLRLRAAASLGAGQWKILTTVILPSTVPFMVTGARIAMGNSFLTVVSA 201 +NT GVQ V + A L +WK+LT ++ PS +P+M+TG R+++G ++L +V+A Sbjct: 191 IINTAVGVQRVPQDYMNVARVLNLSEWKVLTKILFPSVLPYMLTGVRLSIGTAWLVIVAA 250 Query: 202 EIVSAREGLGALIWTARN 219 E+++ G+G +W N Sbjct: 251 EMLTGGVGIGFWVWDEWN 268 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 98.2 bits (243), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 70/200 (35%), Positives = 108/200 (54%) Query: 50 AALELISDGTLQQSVLASSGRILAGWGAGVAIGVPLGIFMGRFDLVRQLLDPYIEFFRFI 109 AA EL + G L + + SSGR +G+ G +G+ LG+ G LLD ++ R I Sbjct: 70 AAWELSASGELWRHLAVSSGRAASGFAVGAGLGLILGLLSGSLRSAELLLDTTLQMVRNI 129 Query: 110 PPIAFVTLAVIWLGPGELSKIALIFYTTVFIVTLNTIAGVQAVNPLRLRAAASLGAGQWK 169 P +A + L ++W G E +K+ L+ F V LNT G+++V+ + A S G W Sbjct: 130 PALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMAKSYGLSGWP 189 Query: 170 ILTTVILPSTVPFMVTGARIAMGNSFLTVVSAEIVSAREGLGALIWTARNFSRTEWVFVG 229 + VILP +P ++ G R A+G ++ ++ AE +SA+ G+G + AR F +T+ V VG Sbjct: 190 LYRDVILPGALPAILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAREFLQTDVVLVG 249 Query: 230 IIALGLLGYLFDRVLRIATR 249 I+ LLG L D R R Sbjct: 250 ILLYALLGKLADTAARWLER 269 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 778,704 Number of extensions: 31633 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 18 Length of query: 256 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 173 Effective length of database: 1,226,865 Effective search space: 212247645 Effective search space used: 212247645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)