BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0049.p (957 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 1986 0.0 gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase ... 290 4e-79 gi|300311101|ref|YP_003775193.1| NADH dehydrogenase I subunit G ... 203 7e-53 gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta... 140 6e-34 gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase... 122 1e-28 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 1986 bits (5145), Expect = 0.0, Method: Compositional matrix adjust. Identities = 942/957 (98%), Positives = 954/957 (99%) Query: 1 MNQLNETDYGTPARASEQMVTLEIDGVAVTVPAGTSVMRASVEAGINVPKLCATDSLEPF 60 MNQLNETDYGTPARAS+ MVTLEIDGVAVTVPAGTSVMRASVEAGINVPKLCATDSLEPF Sbjct: 1 MNQLNETDYGTPARASDNMVTLEIDGVAVTVPAGTSVMRASVEAGINVPKLCATDSLEPF 60 Query: 61 GSCRLCLVEIEKDGRKMKGYPASCTTPCEPGMKVQTQTPKLAEIRRGVMELYISDHPLDC 120 GSCRLCLVEIEKDGRKMKGYPASCTTPCEPGMKVQTQTPKLAEIRRGVMELYISDHPLDC Sbjct: 61 GSCRLCLVEIEKDGRKMKGYPASCTTPCEPGMKVQTQTPKLAEIRRGVMELYISDHPLDC 120 Query: 121 LTCPTNGNCELQDMAGVVGLREVRYGYEGENHTKMKKDESNPYFTYDPSKCIVCNRCVRA 180 LTCPTNGNCELQDMAGVVGLREVRYGYEGENHTKMKKDESNPYFTYDPSKCIVCNRCVRA Sbjct: 121 LTCPTNGNCELQDMAGVVGLREVRYGYEGENHTKMKKDESNPYFTYDPSKCIVCNRCVRA 180 Query: 181 CEETQGTFALTINGRGFESRVSAGQMESFMESECVSCGACVEACPTATLTEKTVIMLGQA 240 CEETQGTFALTINGRGFESRVSAGQME+FMESECVSCGACVEACPTATLTEKTVIMLGQA Sbjct: 181 CEETQGTFALTINGRGFESRVSAGQMEAFMESECVSCGACVEACPTATLTEKTVIMLGQA 240 Query: 241 EHSKVTTCAYCGVGCSFKAEMKGNEVVRMVPYKDGKANQGHSCVKGRFAWGYATHKDRIL 300 EHSKVTTCAYCGVGCSFKAEMKGNEVVRMVPYKDGKANQGHSCVKGRFAWGYATHKDRIL Sbjct: 241 EHSKVTTCAYCGVGCSFKAEMKGNEVVRMVPYKDGKANQGHSCVKGRFAWGYATHKDRIL 300 Query: 301 NPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQKLV 360 NPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQKLV Sbjct: 301 NPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQKLV 360 Query: 361 RAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGHPVF 420 RAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGHPVF Sbjct: 361 RAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGHPVF 420 Query: 421 ASQIKRRVRQGARLIVIDPRTTEMVKSPHLKADYHLKLRPGTNTAIITALAHVILSEGLQ 480 ASQIKRRVRQGA+LIVIDPRTTEMVKSPH++ADYHLKLRPGTNTAIITALAHVILSEGLQ Sbjct: 421 ASQIKRRVRQGAKLIVIDPRTTEMVKSPHIQADYHLKLRPGTNTAIITALAHVILSEGLQ 480 Query: 481 NESYIQERCDLQSYKDWKEFVLREDNSPEAMAAVTGVPAEIIRGAARLYATGGNAAIYYG 540 +E+YIQERCDLQSY+DWKEFVLREDNSPEAMAA+TGVPAEIIRGAARLYATGGNAAIYYG Sbjct: 481 DEAYIQERCDLQSYQDWKEFVLREDNSPEAMAAITGVPAEIIRGAARLYATGGNAAIYYG 540 Query: 541 LGVTEHAQGSTTVMGIANLAMCTGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRH 600 LGVTEHAQGSTTVMGIANLAMCTGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRH Sbjct: 541 LGVTEHAQGSTTVMGIANLAMCTGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRH 600 Query: 601 ISDSTVRGQFEQAWGVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAA 660 ISDSTVR QFEQAWGVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAA Sbjct: 601 ISDSTVRNQFEQAWGVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAA 660 Query: 661 ALQAMECIVVQDLFLNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVRKVMPPKAGKSD 720 ALQAMECIVVQDLFLNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVRKVMPPKAGKSD Sbjct: 661 ALQAMECIVVQDLFLNETAKYAHVFLPGSSFLEKDGTFTNAERRISRVRKVMPPKAGKSD 720 Query: 721 WEVTVALAEALGYPMPYNHPSEIMDEIAALTPSFHGVSYDKLEKLGSIQWPCNEAAPEGT 780 WEVTVALAEALGYPMPYNHPSEIMDEIAALTPSFHGVSYDKLEKLGSIQWPCNE+APEGT Sbjct: 721 WEVTVALAEALGYPMPYNHPSEIMDEIAALTPSFHGVSYDKLEKLGSIQWPCNESAPEGT 780 Query: 781 PIMHVGSFIRGKGKFINTQYFATDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQWHS 840 PIMHVGSFIRGKGKFINTQYFATDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQWHS Sbjct: 781 PIMHVGSFIRGKGKFINTQYFATDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQWHS 840 Query: 841 EDRLEIHPHDAEDRGIREGDWVGVESRAGQTVLRATVTEKVQPGVVYTTFHFPESGANVI 900 EDRLEIHPHDAEDRGIREGDWVGVESRAGQTVLRATVTEKVQPGVVYTTFHFPESGANVI Sbjct: 841 EDRLEIHPHDAEDRGIREGDWVGVESRAGQTVLRATVTEKVQPGVVYTTFHFPESGANVI 900 Query: 901 TTDNSDWATNCPEYKVTAVQVMPVTQPSQWQQHYERFNRAQLDLLKGEAQAEPLVTK 957 TTDNSDWATNCPEYKVTAVQVMPVTQPSQWQQHY+RFNR QLDLLKG+AQAEPLVTK Sbjct: 901 TTDNSDWATNCPEYKVTAVQVMPVTQPSQWQQHYQRFNREQLDLLKGQAQAEPLVTK 957 >gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 922 Score = 290 bits (742), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 207/725 (28%), Positives = 335/725 (46%), Gaps = 51/725 (7%) Query: 237 LGQAEHSKVTTCAYCGVGCSFKAEMKGNEVVRMVPYKDGKANQGHSCVKGRFAWGYATHK 296 L A + TTC YCGVGC A MK + + + AN G CVKG A G Sbjct: 8 LALAVATTSTTCPYCGVGCGVSASMKDDGRIGIQGDAQHPANFGRLCVKGS-ALGETVDL 66 Query: 297 D-RILNPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYL 355 D R+L+P +R + ++++W++AL + AS+F I A+HG D++ S + E+ Y+ Sbjct: 67 DGRLLHPQLRDA-QGQLQQLAWDQALDHVASKFSAIIAEHGPDAVALYVSGQLLTEDYYI 125 Query: 356 VQKLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAA 415 KL++ G+ N+DT +R+C S G K+ GE +E + +D+++++G+N A Sbjct: 126 ANKLMKGYIGSANIDTNSRLCMSSAVAGHKRAFGEDLVPVCYEDLELADLVVLVGSNAAW 185 Query: 416 GHPVFASQIKR--RVRQGARLIVIDPRTTEMVKSPHLKADYHLKLRPGTNTAIITALAHV 473 HP+ +I R R RL+VIDPR T + AD HL ++PGT+ + L Sbjct: 186 CHPILFQRIARLKETRPALRLVVIDPRRTATCE----LADLHLPIKPGTDVWLFNGLMSY 241 Query: 474 ILSEGLQNESYIQERCDLQSYKDWKEFVLRED---NSPEAMAAVTGVPAEIIRGAARLYA 530 + GL++ ++I+ K + E + D P +A + + + + +A Sbjct: 242 LAHRGLRDNAFIEAHT-----KGFDEALAVADADCADPATVARLCKIDLKDLLAFYEAFA 296 Query: 531 TGGNAAIYYGLGVTEHAQGSTTVMGIANLAMCTGNVGREGVGVNPLRGQNNVQGSCDMGS 590 + +GV + + G+ V I N + TG +G+ G+G + GQ N G ++G Sbjct: 297 NTEKVVTGFSMGVNQSSAGTDKVNSIINCHLATGRIGKPGMGPFSITGQPNAMGGREVGG 356 Query: 591 FPHELPGYRHISDSTVRGQFEQAW-GVTLNPEPGLRIPNMFDAALDGSFKGLYCEGEDIV 649 + L + + R + W + +PGL+ +MF A +G K ++ + + Sbjct: 357 LANMLAAHMDLDQPLHRQIVQDFWQSPRIADKPGLKAVDMFRAIEEGRIKAVWIMATNPM 416 Query: 650 QSDPNTQHVAAALQAMECIVVQDLFLN-ETAKYAHVFLPGSSFLEKDGTFTNAERRISRV 708 S P+ V ALQ E +V D+ +T AHV LP ++ EKDGT TN+ERRISR Sbjct: 417 VSLPDADLVQRALQKCELVVSSDVITQTDTNALAHVLLPALAWGEKDGTVTNSERRISRQ 476 Query: 709 RKVM-PPKAGKSDWEVTVALAEALGYP-MPYNHPSEIMDEIAALT--------------- 751 R + P + DW + +A +G+ + P EI DE A L+ Sbjct: 477 RAFLSAPGEARGDWRIICDVARRMGFDGFDFAGPHEIFDEHARLSAHRNGEADASRVRRV 536 Query: 752 ---PSFHGVSYDKLEKLGSIQWPC---NEAAPEGTPIM---HVGSFIRGKGKFINTQYFA 802 G+ + L IQWP ++ + GT + H S G+ +F+ T + Sbjct: 537 FNLAGLTGMDRAAYDALAPIQWPVQRLSDGSVSGTARVLEDHRFSHADGRARFVPTAPRS 596 Query: 803 TDEKVTQRYPLILTTGRILSQYNVGAQTRRTENSQWH-SEDRLEIHPHDAEDRGIREGDW 861 + YPLIL TGR+ ++ +T R+ H E ++IHP DA G+REG Sbjct: 597 AAHAACEDYPLILNTGRVRDHWHTMTRTGRSATLASHIPESFIDIHPQDALLYGVREGGL 656 Query: 862 VGVESRAGQTVLRATVTEKVQPGVVYTTFHFPESGAN-----VITTDNSDWATNCPEYKV 916 + SR G V R + G V+ H+ A+ + D + PE+K Sbjct: 657 ARISSRWGAMVARVQHGGGIARGSVFVPIHWNGQSASDARVGAVVNPVVDPVSGEPEFKH 716 Query: 917 TAVQV 921 T V + Sbjct: 717 TPVAI 721 >gi|300311101|ref|YP_003775193.1| NADH dehydrogenase I subunit G [Herbaspirillum seropedicae SmR1] Length = 772 Score = 203 bits (516), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 130/421 (30%), Positives = 218/421 (51%), Gaps = 19/421 (4%) Query: 19 MVTLEIDGVAVTVPAGTSVMRASVEAGINVPKLCATDSLEPFGSCRLCLVEIEKDGRKMK 78 MV +EIDG V V G+ VM A+ + G +P C L +CR+CLVE+EK + + Sbjct: 1 MVEIEIDGKKVEVQEGSMVMDAANKLGTYIPHFCYHKKLSIAANCRMCLVEVEKAPKPL- 59 Query: 79 GYPASCTTPCEPGMKVQTQTPKLAEIRRGVMELYISDHPLDCLTCPTNGNCELQDMAGVV 138 PA C TP GM V+T + K + ++GVME + +HPLDC C G C+LQD+A V Sbjct: 60 --PA-CATPVNAGMIVRTASDKAVKAQKGVMEFLLINHPLDCPICDQGGECQLQDLA--V 114 Query: 139 GLREVRYGYEGENHTKMKKDESNPYFTYDPSKCIVCNRCVRACEETQGTFALTINGRGFE 198 G Y+ E KD + ++CI C RCVR +E G L + GRG Sbjct: 115 GYGGSSSRYQEEKRVVEPKDVGPLISMKEMNRCIHCTRCVRFGQEVAGVMELGMLGRGEH 174 Query: 199 SRVSAGQMESFMESECVSCGACVEACPTATLTEKTVIMLGQA-EHSKVTTCA-YCGVGCS 256 S ++ ++ ++SE G ++ CP LT K + E S+ + + + VG + Sbjct: 175 SEITTFVGKT-VDSEL--SGNMIDLCPVGALTSKPFRYSARTWELSRRKSVSPHDSVGAN 231 Query: 257 FKAEMKGNEVVRMVPYKDGKANQGHSCVKGRFAWGYATHKDRILNPMIRKSINDPWREVS 316 ++K +V+R++P+++ + N+ K RFA+ + R+ PM+++ + W+EV Sbjct: 232 LIVQVKNGKVMRVLPFENEEVNECWISDKDRFAYEALDSEQRLTKPMLKQ--DGKWQEVD 289 Query: 317 WEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVC 376 W+ AL Y A R I+ +HG D+I + + T EE L+QK+VR G+ N+D R Sbjct: 290 WQTALEYVAHGLRNIRHEHGADAIAALGTPYSTLEELSLLQKIVR-GVGSENIDFRLR-- 346 Query: 377 HSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGHPVFASQIKRRVRQGARLIV 436 + +GL + G E + + + ++G+ HP+ ++++++ + GA++ V Sbjct: 347 --QSDFGLDGQIKPWLGMSIVE-FSQLNRVFVIGSFLRKDHPLLSARLRQSAKSGAKVSV 403 Query: 437 I 437 + Sbjct: 404 L 404 >gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-containing) formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 775 Score = 140 bits (352), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 101/397 (25%), Positives = 168/397 (42%), Gaps = 42/397 (10%) Query: 298 RILNPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQ 357 R+ +PM+ + D ++++ W +A + A + + A D S R +NE +L Q Sbjct: 111 RLTHPMVYDAATDKYKKIEWADAFALMA---KHLNALPDPDMADFYVSGRASNEAAFLFQ 167 Query: 358 KLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGH 417 VR +G NN C+ +CH PT GL T+G GT E D +L+ G NPA H Sbjct: 168 LFVRQ-YGTNNFPDCSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNH 226 Query: 418 PVFASQIKRRVRQGARLIVIDP------------------------RTTEMVKSPHLKAD 453 P +++ ++GA ++ I+P R + M P L D Sbjct: 227 PRMMGELRHASKRGATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGD 286 Query: 454 YHLKLRPGTNTAIITALAHVILSEGLQNESYIQERCDLQSYKDWKEFVLR-EDNSPEAMA 512 L T + A +E + + +I+E ++EF + S + + Sbjct: 287 LALIKGVIKRTIELDDAARAAGAERVIDVKFIEEHT-----AGFEEFAAQARAESWDDIV 341 Query: 513 AVTGVPAEIIRGAARLYATGGNAAIYYGLGVTEHAQGSTTVMGIANLAMCTGNVGREGVG 572 A +GV E I A++Y G +G+G+T+H T+ ++N+ M GN+GR+G G Sbjct: 342 AESGVSREDIEKLAQVYVKGKAVIATWGMGLTQHKHAVATIQLLSNMMMLRGNIGRQGAG 401 Query: 573 VNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVRGQFEQAWGVTLNPEPGLRIPNMFDA 632 + P+RG +NVQG +G P + + EQ +G G A Sbjct: 402 LCPVRGHSNVQGDRTVGIDEKPTPAF--------LDRLEQVFGFKAPRHHGNDTVGSVMA 453 Query: 633 ALDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMECIV 669 L+G K G + + P+T A+++ V Sbjct: 454 MLEGRTKVFIGLGGNFAMATPDTPRTFEAMRSCNLTV 490 >gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 790 Score = 122 bits (307), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 106/405 (26%), Positives = 173/405 (42%), Gaps = 59/405 (14%) Query: 298 RILNPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQ 357 R+ PM + +D + VSW EA A++ R + K + S R + E +YL Q Sbjct: 132 RVTEPMKYDAASDRYLPVSWSEAFDDIAAQLRAMDPK----KVVFYASGRASLETSYLYQ 187 Query: 358 KLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGH 417 + R +G NN+ + +CH T L QT+G GT T + ++D IL G N Sbjct: 188 LMARM-YGTNNLPDSSNMCHESTSVALPQTIGVPVGTVTLDDFGQTDCILFFGHNTGTNA 246 Query: 418 PVFASQIKRRVRQGARLIVIDP-------------RTTEMVKSPHLK-ADYHLKLRPGTN 463 P ++ ++G +I +P EMV H + +L+++ G + Sbjct: 247 PRMLHPLEEVRKRGVPVITFNPLRERGLERFVNPQSPKEMVTPAHTDISTQYLQIKIGGD 306 Query: 464 TAIITALAHVIL-------SEGLQ---NESYIQERCD-LQSYKD------WKEFVLREDN 506 +A +A IL EGL + ++I+E + + D W E Sbjct: 307 SAAAIGMAKRILERDDKARQEGLPRLLDLAFIEEHTSGFEQFADAVRAAPWDEL------ 360 Query: 507 SPEAMAAVTGVPAEIIRGAARLYATGGNAAIYYGLGVTEHAQGSTTVMGIANLAMCTGNV 566 E A V+ E+ AA YA G A + YG+GVT+H + T+ + NL + GN+ Sbjct: 361 --ERHAGVSRAELEL---AADTYAGAGRAMLMYGMGVTQHREAVRTIHMLTNLLLMRGNI 415 Query: 567 GREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVRGQFEQAWGVTLNP--EPGL 624 G+ G G+ P+RG +NVQG +G E P + + +R + P E GL Sbjct: 416 GKPGAGICPIRGHSNVQGQRTVGI--TEKPEL--VPNDKLRALYH------FEPPMEKGL 465 Query: 625 RIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMECIV 669 + DG + G + V++ P+ + AA + + V Sbjct: 466 NTVEACEKIRDGELSAFFMLGGNFVRAIPDHGVMEAAWRKLPLTV 510 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,016,321 Number of extensions: 126649 Number of successful extensions: 321 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 301 Number of HSP's successfully gapped: 6 Length of query: 957 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 863 Effective length of database: 1,174,780 Effective search space: 1013835140 Effective search space used: 1013835140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)