BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0035.p (302 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310792|ref|YP_003774884.1| LysR family transcription regul... 542 e-155 gi|300311724|ref|YP_003775816.1| LysR family transcription regul... 160 2e-40 gi|300311542|ref|YP_003775634.1| LysR family transcription regul... 148 7e-37 gi|300312961|ref|YP_003777053.1| transcription regulator protein... 112 5e-26 gi|300313102|ref|YP_003777194.1| LysR family transcription regul... 101 6e-23 >gi|300310792|ref|YP_003774884.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 302 Score = 542 bits (1396), Expect = e-155, Method: Compositional matrix adjust. Identities = 278/302 (92%), Positives = 291/302 (96%) Query: 1 MRINIETAELQAFLAVARQSSFRAAAEGLFITAPALSRRIENLEQALQERLFDRTTRRVS 60 MRINIETAELQAF+AVARQSSFRAAAEGLFIT PALSRRIENLEQALQERLFDRTTRRVS Sbjct: 1 MRINIETAELQAFIAVARQSSFRAAAEGLFITQPALSRRIENLEQALQERLFDRTTRRVS 60 Query: 61 LTPAGELFLVHAQAVMDELELAVRSVEQGMAHRREHVTVACVPSVANNLLPLVLKPYART 120 LTPAGELFLVHAQAVM+ELELAV+SVEQ MA RREHVTVACVPSVANNLLP VLK YAR+ Sbjct: 61 LTPAGELFLVHAQAVMEELELAVKSVEQRMAQRREHVTVACVPSVANNLLPQVLKRYARS 120 Query: 121 HPNVRVRIIDENAAEVLESVRKGEADFGVNFIGAQEAEIDFRELTRERYLAVMPAHHPLA 180 H NVRVRIIDE+AA+VLESVRKGEADFGVNFIGAQEAEIDF ELTRERYLAVMP HHPLA Sbjct: 121 HANVRVRIIDESAADVLESVRKGEADFGVNFIGAQEAEIDFAELTRERYLAVMPTHHPLA 180 Query: 181 AQSSLHWKSLAGEKLVSVSSSSGNRSLIDNAFSRVPQRPVIQYETNHVAGAISLVAAGLG 240 A +SL+WKSLAGEKLVSVSSSSGNRSLIDNAFSRVPQRPVIQYETNHVAGAISLVAAGLG Sbjct: 181 AHASLNWKSLAGEKLVSVSSSSGNRSLIDNAFSRVPQRPVIQYETNHVAGAISLVAAGLG 240 Query: 241 VALLPELAIRNAPYKGIVARPVTSPVLSRAMGLITLKGRVLKPAAATLAQALREAMASKP 300 VALLPELAIRNAPYKGIVARPVTSPVLSRAMGLITLKGRVLKPAAATLAQALR+A++++ Sbjct: 241 VALLPELAIRNAPYKGIVARPVTSPVLSRAMGLITLKGRVLKPAAATLAQALRDAISTRS 300 Query: 301 RK 302 K Sbjct: 301 GK 302 >gi|300311724|ref|YP_003775816.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 297 Score = 160 bits (404), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 105/297 (35%), Positives = 166/297 (55%), Gaps = 10/297 (3%) Query: 3 INIETAELQAFLAVARQSSFRAAAEGLFITAPALSRRIENLEQALQERLFDRTTRRVSLT 62 + I+ +QAF+A+A SF+ AA L +T A++ R+ LE L L +RTTR ++LT Sbjct: 1 MKIDILGVQAFVAIADHGSFQGAAGALHVTQTAVTLRLRKLEDFLGVTLIERTTRSIALT 60 Query: 63 PAGELFLVHAQAVMDELELAVRSVEQGMAHRREHVTVACVPSVANNLLPLVLKPYARTHP 122 G FL A+ ++ EL A+ + + RR V++ACVP+V LP +L+ Y+ +P Sbjct: 61 EIGRDFLPQARRLLAELAGALVEIRETGIARRGDVSIACVPTVGVQYLPRILQAYSARYP 120 Query: 123 NVRVRIIDENAAEVLESVRKGEADFGVNFIGAQEAEIDFRELTRERYLAVMPAHHPLAAQ 182 + R++I+D ++ V E+V + E +FG++ G E+ LT +RY+ + P HPLA + Sbjct: 121 HNRIKILDHASSAVAEAVLRREVEFGIHIAGEHHPELISVALTEDRYVLICPKDHPLARR 180 Query: 183 SSLHWKSLAGEKLVSVSSSSGNRSLID-----NAFSRVPQRPVIQYETNHVAGAISLVAA 237 + W+ L L+ S NR+L+D N F+ R YE + A+ LVA Sbjct: 181 RRIAWRQLQSYPLIFAGEVSSNRALLDPTLKANGFA---LRSF--YEVQRSSTAVGLVAQ 235 Query: 238 GLGVALLPELAIRNAPYKGIVARPVTSPVLSRAMGLITLKGRVLKPAAATLAQALRE 294 G+GVA++P+LAI+ Y + + PV+SR++ LIT K L PAA L +RE Sbjct: 236 GVGVAVVPKLAIQEGAYPTVRVIELVDPVVSRSLVLITRKAAHLSPAAQALYDMIRE 292 >gi|300311542|ref|YP_003775634.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 335 Score = 148 bits (373), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 99/292 (33%), Positives = 155/292 (53%), Gaps = 9/292 (3%) Query: 1 MRINIETAELQAFLAVARQSSFRAAAEGLFITAPALSRRIENLEQALQERLFDRTTRRVS 60 M+ NI T LQAFLA+A F AA+ ++ A S I+ +E A RLF+R TR V+ Sbjct: 1 MQTNISTRLLQAFLALADCRHFGHAAQRCHVSQSAFSAMIQKIEAAAGARLFERDTRNVT 60 Query: 61 LTPAGELFLVHAQAVMDELELAVRSVEQGMAHRREHVTVACVPSVANNLLPLVLKPYART 120 LTP GELF+ A+ ++ ++E A + +A R+ V +A +PS+A LP VL Y + Sbjct: 61 LTPEGELFVQVARQLVSDIEAAFSDMSDYVARRKGRVAIAALPSLAAGWLPPVLADYRQR 120 Query: 121 HPNVRVRIIDENAAEVLESVRKGEADFGVNFIGAQEAEIDFRELTRERYLAVMPAHHPLA 180 +P V V + D + + L+ +R G+ D + G E D R L + + V H LA Sbjct: 121 YPGVTVELFDAISDQCLDLLRHGKVDIALTAPGPNLLEFDTRPLCSDPFYLVCRKDHKLA 180 Query: 181 AQSSLHWKSLAGEKLVSVSSSSGNRSLIDNAFSRVPQRP--VIQ--YETNHVAGAISLVA 236 ++ + LAG +++ ++ S+ R +D RP VI E H+A +L+ Sbjct: 181 SKRRIKVPQLAGCEMIHLARSTSVRQHLDAVL-----RPGGVIHTGLEVEHLATVAALIE 235 Query: 237 AGLGVALLPELAIRNAPYKGIVARPVTSPVLSRAMGLITLKGRVLKPAAATL 288 +GLGV+++PEL + +VA P+ +P L R + ++T K R L AA L Sbjct: 236 SGLGVSVVPELTLFQFRLPNLVAIPLDAPELKRPLLIVTPKERSLSVAAQGL 287 >gi|300312961|ref|YP_003777053.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 112 bits (279), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 89/276 (32%), Positives = 136/276 (49%), Gaps = 20/276 (7%) Query: 5 IETAELQAFLAVARQSSFRAAAEGLFITAPALSRRIENLEQALQERLFDRTTRRVSLTPA 64 +E L+ F AVA SF AAE L I P LSR+I LE+ L L DR +R ++LT A Sbjct: 1 MELRHLKYFNAVASTLSFSRAAELLHIAQPPLSRQIRQLEEMLGVELIDRASRPIALTMA 60 Query: 65 GELFLVHAQAVMDELELAVRSVEQG---MAHRREH-VTVACVPSVANNLLPLVLKPYART 120 G+ F V+ LE +E+G +A R+H + VPS LLP ++K + Sbjct: 61 GKFFYEQTLQVLSRLE----QIEEGTRVIARGRQHWFNIGFVPSALYGLLPEMIKRFRTE 116 Query: 121 HPNVRVRIIDENAAEVLESVRKGEADFGVNFIGAQEAEIDFRELTRERYLAVMPAHHPLA 180 P + + + E +E+++ G D G + + +I + E +AV P HPL Sbjct: 117 MPEIEIGFSEIVTMEQVEALKSGRIDVGFGRLPISDPDIVCETIVEEPLVAVFPLGHPLL 176 Query: 181 AQSSLHWKSLAGEKLVSVSS----SSGNRSLIDNAFSRVPQRPVIQYETNHVAGAISLVA 236 A++ + LA ++ + + S ++ L + FS RP I E N + AI LVA Sbjct: 177 ARNKVTLAQLAAQRFILYPARPRPSYADQVL--DIFSSRALRPSIVKEANEMQTAIGLVA 234 Query: 237 AGLGVALLPELAIRNAPYKGIVARP-----VTSPVL 267 AG+GV L+P +++ +V RP V SPV+ Sbjct: 235 AGVGVTLVPR-SVQGLHRSDVVYRPLSNKGVHSPVI 269 >gi|300313102|ref|YP_003777194.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 101 bits (252), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 77/248 (31%), Positives = 116/248 (46%), Gaps = 12/248 (4%) Query: 5 IETAELQAFLAVARQSSFRAAAEGLFITAPALSRRIENLEQALQERLFDRTTRRVSLTPA 64 IE L F VA F AAE L I+ P L+R+I LE+ L +LFDR+ R + LT A Sbjct: 2 IELRHLTYFRTVAETLHFGRAAELLHISQPPLTRQIAALEKELGAQLFDRSKRAIQLTAA 61 Query: 65 GELFLVHAQAVMDELELAVRSVEQGMAHRREHVTVACVPSVANNLLPLVLKPYARTHPNV 124 G+ F + LE A R+V + + V + S A N+LP V + Y+ +P V Sbjct: 62 GKYFYRDTTEIFKALERAKRNVSSSSTGKSGALKVGFMMSTAYNILPSVTRHYSAAYPEV 121 Query: 125 RVRIIDENAAEVLESVRKGEADFGVNFIGAQEAEIDFRELTRERYLAVMPAHHPLAAQSS 184 +R+ + + + + D G+ + +D + E LAV+P H LA + S Sbjct: 122 DMRLTEYVPNLLATDIEDEKIDVGIMYRPEDCRRLDSHTIYAEPLLAVLPRDHRLAGRQS 181 Query: 185 LHWKSLAGEKLVSVSSSSGNRSLIDNAFSRVPQ-------RPVIQYETNHVAGAISLVAA 237 + LA + +S+ R + + + Q RP I ETN ++LV Sbjct: 182 ISAAELAEDAFISIP-----RMIAPVVYDLIQQHCQLHGFRPRIVLETNLQQTIVNLVGE 236 Query: 238 GLGVALLP 245 GLGVAL+P Sbjct: 237 GLGVALVP 244 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 715,272 Number of extensions: 24902 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 87 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 87 Length of query: 302 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 217 Effective length of database: 1,217,395 Effective search space: 264174715 Effective search space used: 264174715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)