BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0033.p (388 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 675 0.0 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 588 e-169 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 259 3e-70 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 253 2e-68 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 251 8e-68 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 675 bits (1742), Expect = 0.0, Method: Compositional matrix adjust. Identities = 343/364 (94%), Positives = 354/364 (97%) Query: 5 TKTSCTAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISRTLFPSDNEFVSLILTFGTF 64 T T+ AKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYIS+T FPSDNEFVSLILTFGTF Sbjct: 2 TTTTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTF 61 Query: 65 GAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGITAPILVLT 124 GAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIG+ APILVLT Sbjct: 62 GAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLT 121 Query: 125 GRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQWLSP 184 GRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQ LSP Sbjct: 122 GRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSP 181 Query: 185 LQISEWGWRIPFFIGCMIVPAIFIIRRSLQETEEFMARKHHPGFAEIMASIAQNWKIVVA 244 +QIS+WGWRIPFFIGCMIVPAIFIIRRSLQET+EF+ARK HP F EIM+SIAQNWKIVVA Sbjct: 182 VQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVA 241 Query: 245 GMMMVVMTTVSFYLITVYTPTFGKTVLKLSASDSLIVTFCVGLSNFCWLPVMGALSDRVG 304 GMMMVVMTTVSFYLITVYTPTFGK VLKLSASDSLIVTFCVGLSNFCWLP+MGALSDRVG Sbjct: 242 GMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVG 301 Query: 305 RRPVLVAFTLLTLLTAYPMMSWLVADISFKNLLITELWLSFLYASYNGAAVVALTEVMPA 364 RRPVLVAFTLLTLLTAYP+MSWLV+DISFKNLLITELWLSFLYASYNGAAVVALTEVMPA Sbjct: 302 RRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPA 361 Query: 365 HVRT 368 HVRT Sbjct: 362 HVRT 365 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 588 bits (1515), Expect = e-169, Method: Compositional matrix adjust. Identities = 283/362 (78%), Positives = 318/362 (87%) Query: 7 TSCTAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISRTLFPSDNEFVSLILTFGTFGA 66 T+ TA SK STVLRVTSGNF+EMFDFFL+GFYA++IS+ FPSDNEF SL+LTF TFGA Sbjct: 2 TTSTASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGA 61 Query: 67 GFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGITAPILVLTGR 126 GFLMRPLGAIILG+Y+DRVGRRQGLIVTLA+MA+GT+LIAFVP YATIG AP+LVL GR Sbjct: 62 GFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGR 121 Query: 127 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQWLSPLQ 186 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQ AI+ +A IGYL+S L+P + Sbjct: 122 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAE 181 Query: 187 ISEWGWRIPFFIGCMIVPAIFIIRRSLQETEEFMARKHHPGFAEIMASIAQNWKIVVAGM 246 +S WGWRIPFFIGC+IVP +F+IRRSLQETEEFM RKH P +I S+ +NWK+V+AGM Sbjct: 182 VSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGM 241 Query: 247 MMVVMTTVSFYLITVYTPTFGKTVLKLSASDSLIVTFCVGLSNFCWLPVMGALSDRVGRR 306 M+V MTTVSFYLITVYTPTFGK VLKLS SLIVT CVGLSNF WLP+MGALSDRVGRR Sbjct: 242 MLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRR 301 Query: 307 PVLVAFTLLTLLTAYPMMSWLVADISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHV 366 P+LV FT+LT+LTAYP ++WLV D SF +LI ELWLSFLYASYNGA VVALTEVMPA V Sbjct: 302 PLLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADV 361 Query: 367 RT 368 RT Sbjct: 362 RT 363 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 259 bits (661), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 138/357 (38%), Positives = 203/357 (56%), Gaps = 2/357 (0%) Query: 14 SKLSTVLRVTSGNFLEMFDFFLYGFYASYISRTLFPSDNEFVSLILTFGTFGAGFLMRPL 73 +K V+ GN LE +DF +YGF+++ I+R FP+DNE+ SL++ TFG GF MRP+ Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 74 GAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGITAPILVLTGRLLQGFSA 133 G ++LG Y DR GR+ + V + +M + +IAF P YATIGI API+++ R+LQGF+ Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 134 GVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQWLSPLQISEWGWR 193 G E + +L E A +G Y SWQ Q A+ A +G LV++ LSP + WGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 194 IPFFIGCMIVPAIFIIRRSLQETEEFMARKHHPGFAEI--MASIAQNWKIVVAGMMMVVM 251 +PF +G +I P IRR + ET++F+ + P A I + + N + V+ M V+ Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVIN 245 Query: 252 TTVSFYLITVYTPTFGKTVLKLSASDSLIVTFCVGLSNFCWLPVMGALSDRVGRRPVLVA 311 TV+FY++ V PTF L L + +V +P GALSDR+GRR VL+ Sbjct: 246 GTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLY 305 Query: 312 FTLLTLLTAYPMMSWLVADISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHVRT 368 +L LL YP+ W+ A S LL+ ++ L + + G AL+E P VR+ Sbjct: 306 SSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRS 362 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 253 bits (646), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 138/366 (37%), Positives = 207/366 (56%), Gaps = 3/366 (0%) Query: 2 NTTTKTSCTAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISRTLFPSDNEFVSLILTF 61 NT + ++ + GN LE FD +YG +A I++ FP+ ++ VSL+LTF Sbjct: 4 NTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTF 63 Query: 62 GTFGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGITAPIL 121 TFG F MRPLGA++LG+Y DR GR+ L++++ +M +GT +IAF+P YA+IG+ AP Sbjct: 64 ATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAG 123 Query: 122 VLTGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQW 181 + G+++QGFSAG E G + +L E A P +GF+ SWQ ASQ +++ +AL G +++ Sbjct: 124 IALGKMIQGFSAGGEFGSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAALFGAVLNNM 182 Query: 182 LSPLQISEWGWRIPFFIGCMIVPAIFIIRRSLQETEEFMARKHHPGFAEIMASIAQNWKI 241 L+P Q++ WGWR+PF G +I PA IRR L E EF A + + A Sbjct: 183 LTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTN-APLRDTFAHQKMR 241 Query: 242 VVAGMMMVVMTTVSFYLITVYTPTFGKTVLKLSASDSLIVTFCVGLSNFCWLPVMGALSD 301 ++ G V+M TVS YL ++Y PT+ L L A S GL F P++GALSD Sbjct: 242 LLIGAGSVIMATVSVYL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSD 300 Query: 302 RVGRRPVLVAFTLLTLLTAYPMMSWLVADISFKNLLITELWLSFLYASYNGAAVVALTEV 361 ++GR P ++ + L ++ YPM +L F LL+ + + L Y A L ++ Sbjct: 301 KIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADI 360 Query: 362 MPAHVR 367 P R Sbjct: 361 FPVATR 366 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 251 bits (640), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 136/357 (38%), Positives = 203/357 (56%), Gaps = 3/357 (0%) Query: 11 AKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISRTLFPSDNEFVSLILTFGTFGAGFLM 70 + + ++ GN LE FDF +Y F+A I++ FP+ N+ SL+L TFG GF M Sbjct: 15 SNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFM 74 Query: 71 RPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGITAPILVLTGRLLQG 130 RP+G I+LG Y DRVGR+ L VT+ +MA+GT +I P Y +IG+ AP++++ RLLQG Sbjct: 75 RPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQG 134 Query: 131 FSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQWLSPLQISEW 190 FSAG E+G + +L E A + +Y SW AS A++ A +G V+ L ++ W Sbjct: 135 FSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASW 194 Query: 191 GWRIPFFIGCMIVPAIFIIRRSLQETEEFMARKHHPGFAEIMASIAQNW-KIVVAGMMMV 249 GWR+PF +G +I P + IR L+ET F+ K + + +N+ + A MV Sbjct: 195 GWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTD--SPLKEVIRNFPRETSASFSMV 252 Query: 250 VMTTVSFYLITVYTPTFGKTVLKLSASDSLIVTFCVGLSNFCWLPVMGALSDRVGRRPVL 309 ++ TV Y++ Y PT+ VLKL + I GL P++G L+DR+GRRP+L Sbjct: 253 ILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLL 312 Query: 310 VAFTLLTLLTAYPMMSWLVADISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHV 366 L L+ AYPM +++ + S LLI +L L A+Y G + A +E+ PA V Sbjct: 313 SGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKV 369 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.138 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,086,784 Number of extensions: 42064 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 247 Number of HSP's successfully gapped: 14 Length of query: 388 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 301 Effective length of database: 1,207,925 Effective search space: 363585425 Effective search space used: 363585425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)