BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0031.p (387 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 238 6e-64 gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis pro... 234 9e-63 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 233 2e-62 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 224 1e-59 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 222 4e-59 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 238 bits (607), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 144/366 (39%), Positives = 216/366 (59%), Gaps = 5/366 (1%) Query: 2 LAHLKLGTRLSLGFGLVLALLTGITVLAIVSLFTLHEDTRRIVEDRYPQVLLANDVLLRI 61 +LK+ RL+ F ++ALL ++V A + + ++ +IV DRY +V A D+ + Sbjct: 5 FKNLKVAVRLAAVFAFIVALLAVVSVTAFIKINNINRAIDQIVNDRYLKVRWAFDLRDGV 64 Query: 62 SENASAMRNLLLLDDHQKLTEEIARIAKGEAAITAT---LDQLQARLSSEQGRKSFAEIL 118 +E +R + + +++ E+ R A+ E A+ T LD++ AR + G+K + Sbjct: 65 NEQIKYLRGIAIDIENRPANEK--RFAQLEVAVKQTKEALDKITARQVTAVGQKKAKALD 122 Query: 119 ALRSKYHEGQQRFLQLASTGATMDATEFLLTTLRQDQENTIVRLKGYIAVGTRMMERSGE 178 R + + FL L G + A E++L + Q N + + + ++ G Sbjct: 123 EARIAFDASKADFLALVKAGNSTSADEYVLRKITDTQNNYLALARAFADSQDAQLQAEGA 182 Query: 179 EAAAQYHQKTRIMLAIALLAVASGGACAWWITRSITRPVRHAVGVAQQVAHGNLDSRIEQ 238 +A A + LA ++ AV + + + RSI +P+ AV VA+ VA G+L +RIE Sbjct: 183 KAVADGAMAIEVTLACSMAAVLAAIILGYLMARSIVKPLTEAVRVAENVAAGDLTTRIEP 242 Query: 239 GGRDELGQLLEALHQMNGSLTGIVGEVRKGAETIATASSEIAGGNLDLSSRTEQQAGSLE 298 RDE GQL++AL +MN +L IV VR+G E+I TAS EIA GNLDLS+RTEQQAGSLE Sbjct: 243 HSRDETGQLMQALRKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLSARTEQQAGSLE 302 Query: 299 QTAAAMEELTSTVRQTADNAKAANQLAAEAATVAARSGAVVQEVVATMAGISQASCRIVD 358 +TA+AMEE+TSTVRQ ADNA+ ANQ+A A+ +A + G +V VV TM I+Q+S +IVD Sbjct: 303 ETASAMEEMTSTVRQNADNARQANQMAVSASEIALQGGQIVGRVVHTMDEINQSSRKIVD 362 Query: 359 IIGVID 364 IIGVID Sbjct: 363 IIGVID 368 >gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 534 Score = 234 bits (597), Expect = 9e-63, Method: Compositional matrix adjust. Identities = 146/363 (40%), Positives = 217/363 (59%) Query: 2 LAHLKLGTRLSLGFGLVLALLTGITVLAIVSLFTLHEDTRRIVEDRYPQVLLANDVLLRI 61 L+++ + RL LGFGL++ L+ + I L L+ + ++ D+YP+ ++A D++ ++ Sbjct: 3 LSNVTIRVRLWLGFGLLMVLMLAMAASGIARLGQLNAQMKDVIHDKYPKTVVAGDIVDQL 62 Query: 62 SENASAMRNLLLLDDHQKLTEEIARIAKGEAAITATLDQLQARLSSEQGRKSFAEILALR 121 + A ++RN+LLL ++ E RI+ + AI L QL L+ R + ++ + Sbjct: 63 NLIARSVRNILLLKREPQIKSEYERISGADRAIREDLAQLDKLLTDGDSRAALTTLVTAQ 122 Query: 122 SKYHEGQQRFLQLASTGATMDATEFLLTTLRQDQENTIVRLKGYIAVGTRMMERSGEEAA 181 + Y E + + L + G A + L+ +R Q T+ I MM+ +G+E Sbjct: 123 NAYFEKRDKVLAMVQQGGKEGAIDMLIDEVRPVQSATMNAADALIRQQHEMMKTAGDEVE 182 Query: 182 AQYHQKTRIMLAIALLAVASGGACAWWITRSITRPVRHAVGVAQQVAHGNLDSRIEQGGR 241 + Y Q ++L +A +A+ AW ITRSIT P+ AV VA+ VA G+L S I+ GR Sbjct: 183 SNYAQARTLLLLLAAAGLAAAAVIAWMITRSITAPLDRAVRVAETVAAGDLTSAIDAHGR 242 Query: 242 DELGQLLEALHQMNGSLTGIVGEVRKGAETIATASSEIAGGNLDLSSRTEQQAGSLEQTA 301 DE LL AL +MN L IV +VR +TIATASSEIA GNLDLSSRTE+QA SLE+TA Sbjct: 243 DETSMLLRALKKMNDRLLDIVSQVRVSTDTIATASSEIARGNLDLSSRTEEQAASLEETA 302 Query: 302 AAMEELTSTVRQTADNAKAANQLAAEAATVAARSGAVVQEVVATMAGISQASCRIVDIIG 361 ++ME+LT+TV+Q A +A A+QLA A+ +A + G VV VV TM I +S +IVDIIG Sbjct: 303 SSMEQLTATVKQNASHAGQASQLAGSASDIARQGGEVVGHVVETMGAIDASSKKIVDIIG 362 Query: 362 VID 364 VID Sbjct: 363 VID 365 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 233 bits (594), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 153/363 (42%), Positives = 222/363 (61%) Query: 2 LAHLKLGTRLSLGFGLVLALLTGITVLAIVSLFTLHEDTRRIVEDRYPQVLLANDVLLRI 61 L +K+ RL++ F +++ ++ IT + + SL ++ + + IVEDRY ++ LA ++ + Sbjct: 3 LKDIKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRDNV 62 Query: 62 SENASAMRNLLLLDDHQKLTEEIARIAKGEAAITATLDQLQARLSSEQGRKSFAEILALR 121 + A +RN LL + ++ T + R A A T L +++ LS+ +GR+ F I R Sbjct: 63 NGAARNLRNALLGRNLEEATRYLDRSAANSAKTTEALGKMEKILSTARGRELFQSIQEAR 122 Query: 122 SKYHEGQQRFLQLASTGATMDATEFLLTTLRQDQENTIVRLKGYIAVGTRMMERSGEEAA 181 S Y+ + + +L +A E L T + Q+ L ++A +ME+S E Sbjct: 123 SAYNTPRDKLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSVTEGR 182 Query: 182 AQYHQKTRIMLAIALLAVASGGACAWWITRSITRPVRHAVGVAQQVAHGNLDSRIEQGGR 241 +ML ++ +A+A AW +TRSITRP+ AV VA VA G+L +I + Sbjct: 183 DTTQSAIAMMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQIGATSK 242 Query: 242 DELGQLLEALHQMNGSLTGIVGEVRKGAETIATASSEIAGGNLDLSSRTEQQAGSLEQTA 301 DE G LL AL MN SL IV EVR+G++TI TAS+EIA GNLDLSSRTE+QAG+LE+TA Sbjct: 243 DETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGALEETA 302 Query: 302 AAMEELTSTVRQTADNAKAANQLAAEAATVAARSGAVVQEVVATMAGISQASCRIVDIIG 361 +AMEELTSTV+Q ADNA+ AN LAA A+ VA + G+VV +VV TM I+ AS +IVDII Sbjct: 303 SAMEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKIVDIIS 362 Query: 362 VID 364 VID Sbjct: 363 VID 365 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 224 bits (570), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 145/366 (39%), Positives = 211/366 (57%), Gaps = 6/366 (1%) Query: 2 LAHLKLGTRLSLGFGLVLALLTGITVLAIVSLFTLHEDTRRIVEDRYPQVLLANDVLLRI 61 + +LK+G RL++GF +L L+ + L + L ++ + T + + A I Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 Query: 62 SENASAMRNLLLLDDHQKLTEEIARIAKGEAAITATLDQLQARLSSEQGRKSFAEILALR 121 EN ++ DD +I A I+ +++A ++ + ++ F E+ LR Sbjct: 61 KENGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKLR 120 Query: 122 SKYHEGQQRFLQLASTGATMDATEFLLTTLRQ---DQENTIVRLKGYIAVGTRMMERSGE 178 + Y +Q + + G A E TTL + D + ++R Y ++++++ Sbjct: 121 AAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLR---YADFQKQVIDQTAA 177 Query: 179 EAAAQYHQKTRIMLAIALLAVASGGACAWWITRSITRPVRHAVGVAQQVAHGNLDSRIEQ 238 Y ++LA+ + + G A AW +TRSITRP+ AV +A+ VA G+L SRI+ Sbjct: 178 AINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDS 237 Query: 239 GGRDELGQLLEALHQMNGSLTGIVGEVRKGAETIATASSEIAGGNLDLSSRTEQQAGSLE 298 RDE G+LL AL MNG+L IVG VR G ETI TAS EIA GNLDLS+RTE QAGSLE Sbjct: 238 SSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLE 297 Query: 299 QTAAAMEELTSTVRQTADNAKAANQLAAEAATVAARSGAVVQEVVATMAGISQASCRIVD 358 +TA+AME+LTSTV+Q ADNA+ AN+LAA A+ VA + GAVV +VVATM I +S +I D Sbjct: 298 ETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIAD 357 Query: 359 IIGVID 364 IIGVID Sbjct: 358 IIGVID 363 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 222 bits (566), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 130/361 (36%), Positives = 216/361 (59%) Query: 4 HLKLGTRLSLGFGLVLALLTGITVLAIVSLFTLHEDTRRIVEDRYPQVLLANDVLLRISE 63 +L + TRL++ F +++ ++ GI L + L +++ +V DRY ++ + N + ++ + Sbjct: 5 NLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISGKVDD 64 Query: 64 NASAMRNLLLLDDHQKLTEEIARIAKGEAAITATLDQLQARLSSEQGRKSFAEILALRSK 123 A ++RN LL+ D ++ E I A T D+L +++ + + ++ R Sbjct: 65 VAISLRNQLLMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLDKVTETRKS 124 Query: 124 YHEGQQRFLQLASTGATMDATEFLLTTLRQDQENTIVRLKGYIAVGTRMMERSGEEAAAQ 183 Y +++ +L S+G A + +L+ + Q+ +L ++M++S ++A A Sbjct: 125 YAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKLMDQSVDQAEAA 184 Query: 184 YHQKTRIMLAIALLAVASGGACAWWITRSITRPVRHAVGVAQQVAHGNLDSRIEQGGRDE 243 Y IM+ +LA AW I+RSITRP+ AV VA+ VA G+L + I DE Sbjct: 185 YETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDLTAVIAAHSNDE 244 Query: 244 LGQLLEALHQMNGSLTGIVGEVRKGAETIATASSEIAGGNLDLSSRTEQQAGSLEQTAAA 303 G+LL+AL MN L IV EVR+G +++ TAS++IA GNLDLSSRTE+QA +LE+TA++ Sbjct: 245 TGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEEQASALEETASS 304 Query: 304 MEELTSTVRQTADNAKAANQLAAEAATVAARSGAVVQEVVATMAGISQASCRIVDIIGVI 363 +EELTS+V+Q AD+ + +++LAA A+ VA + G V++VV TM I+ +S +IVDII VI Sbjct: 305 LEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDSSHKIVDIISVI 364 Query: 364 D 364 D Sbjct: 365 D 365 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.129 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 963,217 Number of extensions: 35298 Number of successful extensions: 432 Number of sequences better than 1.0e-08: 65 Number of HSP's gapped: 384 Number of HSP's successfully gapped: 65 Length of query: 387 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 300 Effective length of database: 1,207,925 Effective search space: 362377500 Effective search space used: 362377500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)