BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0030.p (377 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313395|ref|YP_003777487.1| porin transmembrane protein [He... 177 2e-45 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 147 1e-36 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 139 3e-34 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 132 6e-32 gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC fami... 131 9e-32 >gi|300313395|ref|YP_003777487.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 177 bits (448), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 139/379 (36%), Positives = 192/379 (50%), Gaps = 42/379 (11%) Query: 34 FAQSSVTIYGSLDAGVAYINNLNGGSVSRLDQGTMQPDRIGFRGTEDLGGNLKAIFQLEA 93 +AQSSVT+YG++D G+AY +N G S + ++ G + ++G GTEDLGG A+F+LE Sbjct: 32 WAQSSVTLYGTVDLGLAYSSNQGGASNTYMNSGALAASKLGLTGTEDLGGGNMALFRLEN 91 Query: 94 GFYTDSGATPNSSKLFNRYSTIGLSG-DFGSVTLGHMPDVVFDYVGKLSNGFQLTNWYLF 152 GF D+GA + LFNR S +GLS D+G VT+G F YVG L LT Sbjct: 92 GFNADTGAQSTAGVLFNRQSYVGLSSKDYGQVTVGRQYTPYFQYVGALGPTNVLTGATGA 151 Query: 153 HPGNLDSLANTYQFDNAVRYTSPTLGGFQGSLMYGFGEVAGDSSNGRNVSAGGTYTNGPF 212 HPG++D+L T + +N++ YT PTLGG Q + YG GE AG ++NG +VSA Y F Sbjct: 152 HPGDIDALDTTLRMNNSITYTLPTLGGLQAGVQYGMGEQAGSAANGSSVSAALRYDYQAF 211 Query: 213 RAALAYSKLNNRAAG-FAGTF--------LTSVGLGNAATVFNSLTTTAGGLGYTIGNVR 263 + Y++L N A G AG+F + V G A+ L TA YT G++ Sbjct: 212 SWSAGYTRLKNLANGNTAGSFGNVGSFANNSPVNAGYASADSTQLLATAA--RYTRGDLM 269 Query: 264 LNTAYTQVKL---ALPS-------------GATPKQKNLDLGAAWRYATLDTINFGYGLS 307 L Y+ V+ AL + G+ L L A + Y N G+S Sbjct: 270 LGLNYSNVQYKAGALSAFSQTAVFNTIGLIGSYNVSPALVLAAGYSYTAEKARN---GIS 326 Query: 308 KLEGARWNTFSLSHVHEFSKRTQWYVQAAYQRAGGDARFA------VMNGTGASGATG-- 359 A++N SL V+ SKRT Y AYQRA G A ++N + G + Sbjct: 327 --SPAKYNQLSLEEVYSLSKRTALYAIQAYQRASGQTLRAGGVGSSIVNAVASVGDSQNG 384 Query: 360 -VSGGQGQLVTTIGMHHSF 377 S G GQ V +G+ HSF Sbjct: 385 TPSSGSGQFVAMLGIRHSF 403 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 147 bits (371), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 142/406 (34%), Positives = 194/406 (47%), Gaps = 61/406 (15%) Query: 17 MKKLMMAAAA-SCLSAGAFAQSSVTIYGSLDAGVAYINNL---NGGSVSR--LDQGTMQP 70 MKK ++A A + A AQSSVTIYG +D GV Y + + GG+ S+ L+ G +Q Sbjct: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQG 60 Query: 71 DRIGFRGTEDLGGNLKAIFQLEAGFYTDSGA---TPNSSKLFNRYSTIGLSGDFGSVTLG 127 RIGF+GTEDLGG L A+F LEAGF D+GA + S+ LF R S +GL+G+FGSV LG Sbjct: 61 SRIGFKGTEDLGGGLSAVFNLEAGFSNDTGALDDSKTSNTLFRRKSVVGLAGNFGSVLLG 120 Query: 128 HMPDVVFDYVGKLSNGFQLTNWYLFHPGNLDSLANTYQFDNAVRYTSPTLGGFQGSLMYG 187 D D + ++ W NLD L T + +N++ YT+ L GF G+LMYG Sbjct: 121 RQTDFA-DTISSYTSVQDFGGWTGAAGHNLDRLEGT-RTNNSISYTTANLNGFTGNLMYG 178 Query: 188 FGEVAGDSSNGRNVSAGGTYTNGPFRAALAY--SKLNNRAAGFA-----GTFLTSVGLGN 240 FGE AG +S G+ GG Y NGP + Y SK + A + GT + G N Sbjct: 179 FGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATGTNQFTNGTNN 238 Query: 241 AATVFNSLTTTAGGLGYTIGNVRLNTAYTQVKLALP-----------SGATPKQKNLDLG 289 +T L A Y G R+ +++VK L S AT + L Sbjct: 239 GSTAQKVLNVAA---SYQFGPARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTL--- 292 Query: 290 AAWRYATLDTINFGYGLS--------------KLEG----ARWNTFSLSHVHEFSKRTQW 331 AA A + I Y LS + +G A+ SL + SKRT Sbjct: 293 AASNKADIYEIGTAYSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQVSLGTDYWLSKRTDL 352 Query: 332 YVQAAYQRAGGDARFAVMNGTGASGATGVSGGQGQLVTTIGMHHSF 377 Y AY +A +N G + ++G G QG + +G+ H F Sbjct: 353 YAFLAYSKA------TDLNNPGVADSSGTDGNQGAVA--VGIRHKF 390 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 139 bits (351), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 124/379 (32%), Positives = 173/379 (45%), Gaps = 44/379 (11%) Query: 16 LMKKLMMAAAASCLSAGAFAQSSVTIYGSLDAGVAYINNL-----NGGSVSRLDQGTMQP 70 + K L++AA + + A AQSSV+IYG +D G+ Y ++ G + R+D G Q Sbjct: 1 MKKTLLVAAMLAGGATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQG 60 Query: 71 DRIGFRGTEDLGGNLKAIFQLEAGFYTDSGATPNSSKLFNRYSTIGL-SGDFGSVTLGHM 129 RIGF+GTEDLGG + A F LE GF D G+ LF R S +GL S +GS+++G Sbjct: 61 SRIGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQ 120 Query: 130 PDVVFDYVGKLSNGFQLT----NWYLFHPGNLDSLANTYQF-----DNAVRYTSPTLGGF 180 D + + G+ + G W L + N + + +N+++Y S GGF Sbjct: 121 YDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGF 180 Query: 181 QGSLMYGFGEVAGDSSNGRNVSAGGTYTNGPFRAALAYSKLNNRAAGFAGTFLTSVGLGN 240 M G GE AG+++ GR VSA G Y NGPF ALAY+ + + G+F T + Sbjct: 181 SFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKDS----TGSFSTRI---- 232 Query: 241 AATVFNSLTTTAGGLGYTIGNVRLNTAYTQVKLALPSGATPKQKNLDLGAAWRYATLDTI 300 GG Y IG TQVK + + K +LG + L + Sbjct: 233 ----------YGGGARYQIGAFTPFGVVTQVK---NTNTSAKATTYELGTVYALTPLWDL 279 Query: 301 NFGYGLSKLEGARWNTFSLSHV--HEFSKRTQWYVQAAYQRAGGDARFAVMNGTGASGAT 358 Y K N +L ++ SKRT YV A Y R G + V G G AT Sbjct: 280 AAAYQFQKRNQGVGNAQALVATVDYKISKRTDLYVGAVYDRDKGYNAYPVFGG-GIQSAT 338 Query: 359 GVSGGQGQLVTTIGMHHSF 377 G Q +GM H F Sbjct: 339 G-----NQTALRLGMRHRF 352 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 132 bits (331), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 116/356 (32%), Positives = 164/356 (46%), Gaps = 35/356 (9%) Query: 35 AQSSVTIYGSLDAGVAYINNL--NG-GSVSRLDQGTMQPDRIGFRGTEDLGGNLKAIFQL 91 AQSSVTIYG +D GV Y+NN NG GSV+R+ G RIGFRGTEDLGG LKA+FQL Sbjct: 21 AQSSVTIYGLVDTGVEYLNNAGPNGAGSVTRMTSGATNTSRIGFRGTEDLGGGLKALFQL 80 Query: 92 EAGFYTDSGATP-NSSKLFNRYSTIGLSGDFGSVTLGHMPDVVFDYVGKLSN-GFQLTNW 149 E GF D+G+ ++++LFNR ST+GL G FG V G +D++G G+ Sbjct: 81 ENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGPFDPMGYSANYS 140 Query: 150 YLFHPGNLDSLANTYQ--FDNAVRYTSPTLGGFQGSLMYGFGEVAGDSSNGRNVSAGGTY 207 + G + + N V+Y G + MYGFGEVAG++ NG G + Sbjct: 141 WATTAGATGNRKDGMPTGVSNLVKYQGE-FSGVKIGAMYGFGEVAGETRNGAKYGVGLGW 199 Query: 208 TNGPFRAALAYSKLNNRAAGFAGTFLTSVGLGNAATVFNSLTTTAGGLGYTIGNV-RLNT 266 GPF Y + N+ A T ++ T+ GY + N Sbjct: 200 GTGPFSVVATYDRNNSATA--------------TNTPYDRATSAHLAAGYQPSEAWKFNL 245 Query: 267 AYTQVKLALPSGATPKQKNLDL-GAAWRYATLDTINFGYGLSKLEG----ARWNTFSLSH 321 Y K +L +GA + + GAA+++ T+ ++ A +SL Sbjct: 246 GYRYYKKSLATGAADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVANDADPIMYSLRL 305 Query: 322 VHEFSKRTQWYVQAAYQRAGGDARFAVMNGTGASGATGVSGGQGQLVTTIGMHHSF 377 + SKRT Y+ AY +A + G++ Q T+GM H F Sbjct: 306 KYAMSKRTDLYLSTAYAKAKNGQLVGLSRDDAGYGSS-------QFGMTLGMQHRF 354 >gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC family protein [Herbaspirillum seropedicae SmR1] Length = 346 Score = 131 bits (329), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 120/368 (32%), Positives = 166/368 (45%), Gaps = 29/368 (7%) Query: 17 MKKLMMAAAASCLSAG-AFAQSSVTIYGSLDAGVAYINNLNGGSVSRLDQGTMQPDRIGF 75 MKK AS L AG A AQS+VT+YG +D + Y N GSV+ LD G Q R+GF Sbjct: 1 MKKAWAVGVASLLCAGGALAQSNVTLYGVIDTYMGYTNATGKGSVASLDSGGYQASRVGF 60 Query: 76 RGTEDLGGNLKAIFQLEAGFYTDSGATPNSSKLFNRYSTIGLSGDFGSVTLGHMPDVVFD 135 +G+EDLGG L+A FQ+E GF +DSGA +S++LFNR S +GL + G + LG F Sbjct: 61 KGSEDLGGGLRANFQMENGFASDSGAMHDSTRLFNRQSWVGLGNELGELRLGRQNSPGFL 120 Query: 136 YVGKLSNGFQLTNWYLFHPGNLDSL-ANTYQFDNAVRYTSPTLGGFQGSLMYGFGEVAGD 194 + +L + F + F L+++ A T ++DN + Y SP + GF+ YG GE Sbjct: 121 MIARL-DAFAGATYASF----LNNVSAYTPRYDNVIGYLSPVMRGFRVQAYYGLGEQTAP 175 Query: 195 SSNGRNVSAGGTYTNGPFRAALAYSKLNNRAAGFAGTFLTSVGLGNAATVFNSLTTTAGG 254 + G Y +GP ++ S N+ N S T G Sbjct: 176 RNGLSTAMLAGEYESGPLYLGVSSSAQNS---------------ANDNITIRS-TFAGGA 219 Query: 255 LGYTIGNVRLNTAYTQVKLALPSGATPKQ--KNLDLGAAWRYATLDTINFGYGLSKLE-- 310 Y G V L A S TP L A +R T+ GYG +K Sbjct: 220 YDYGSGKVFLGYYRGNNLGAAASANTPGSYYSAYSLSANYRLTGQATLGAGYGWAKDSTG 279 Query: 311 -GARWNTFSLSHVHEFSKRTQWYVQAAYQRAGGDARFAVMNGTGASGATGVSGGQGQLVT 369 G FSL ++ S+RT Y A+ A F++ + VSGG V Sbjct: 280 AGRNARQFSLIGTYDLSRRTMLYATYAHLANNNGASFSLAGAAPITKNAPVSGGTVNGV- 338 Query: 370 TIGMHHSF 377 +G+ H F Sbjct: 339 QVGIRHLF 346 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,116,877 Number of extensions: 48613 Number of successful extensions: 186 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 15 Length of query: 377 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 290 Effective length of database: 1,207,925 Effective search space: 350298250 Effective search space used: 350298250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)