BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0032.0027.p
         (349 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313279|ref|YP_003777371.1| vanillate O-demethylase oxygena...   110   2e-25
gi|300309801|ref|YP_003773893.1| phenylpropionate dioxygenase [H...    91   1e-19

>gi|300313279|ref|YP_003777371.1| vanillate O-demethylase oxygenase
           subunit oxidoreductase [Herbaspirillum seropedicae SmR1]
          Length = 349

 Score =  110 bits (274), Expect = 2e-25,   Method: Compositional matrix adjust.
 Identities = 59/173 (34%), Positives = 92/173 (53%), Gaps = 3/173 (1%)

Query: 18  KDLWYPVCPSEFVAEKPVSLRRLGKKFVFWREPSGKLHALEDHCPHRGAPLSMGIPMGDR 77
           K+ WY  C  + +  KP+  +  G+K VF+R   GK+ A+ED CPHRGAPLS+G     +
Sbjct: 4   KNAWYVACTPDEIDSKPLGRQICGEKIVFYRGAGGKVAAVEDFCPHRGAPLSLGFVREGQ 63

Query: 78  IACGYHGVQVRFDGVVTSVPGSPGCKLEGAKATRRFHVTEAAGAIFLYNSLEDVDTPPPL 137
           + CGYHG+++  DG   S+PG    ++ G    R + V E  G I+++   ++   P  +
Sbjct: 64  LVCGYHGLEMGCDGKAISMPGQ---RVRGFPCIRSYPVEERYGFIWVWPGDKEQADPAQI 120

Query: 138 RLPEELTSPEYSSFLCYTEWKGDYRYVLDNVMDPMHGTFLHKQSHSMAEGDNT 190
              E   +PE++          DYR ++DN+MD  H T++H  S    E D  
Sbjct: 121 HHLEWADNPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEA 173


>gi|300309801|ref|YP_003773893.1| phenylpropionate dioxygenase
           [Herbaspirillum seropedicae SmR1]
          Length = 343

 Score = 91.3 bits (225), Expect = 1e-19,   Method: Compositional matrix adjust.
 Identities = 81/307 (26%), Positives = 123/307 (40%), Gaps = 34/307 (11%)

Query: 17  LKDLWYPVCPSEFVAEKPVSLRRLGKKFVFWREPSGKLHALEDHCPHRGAPLSMGIPMGD 76
           L+  WY + P   + + P     LG+K V W +  G   AL+D C HR A LS G     
Sbjct: 9   LRRFWYALMPIAQLRQGPQPFTLLGEKLVVWLKEDGAPAALQDRCCHRTARLSKGFVENG 68

Query: 77  RIACGYHGVQVRFDGVVTSVPGSPGCKLEGAKATRRFHVTEAAGAIFLYNSLEDVDTPPP 136
            I CGYHG     +G    VP +    +      + +   E  G  +++ +LE+     P
Sbjct: 69  NIVCGYHGWTFNGEGACVRVPQNADGSVPAGACVKSYFCKERYG--YVWVALEE-----P 121

Query: 137 LR----LPEELTSPEYSSFLCYTEWKGDYRYVLDNVMDPMHGTFLHKQSHSMAEGDNTAS 192
           LR     PEE        F  Y  W+     +++N  D  H +++H+ +  + +      
Sbjct: 122 LRDIPEFPEEGAPGYRRIFQFYERWETSALRMMENSFDNSHFSYVHRATFGLYDQPKPEK 181

Query: 193 FGIRETDIGFVFE-KEGQRDVNFDWTEWG---DTGLHWM----------RLAIPYPKTGG 238
           + I ETD GF  E +   ++V       G   DT +  M          R    YP++  
Sbjct: 182 YEIAETDYGFEAETRVPIKNVPASHRVTGSTADTTVRHMFNRWYLPFVRRFGCTYPES-- 239

Query: 239 PGGSFVIIGSYTPVVDNIAAVFHWRCRKLSGWQRDTWRFLYRNRLEARHWNVLEQDRVVL 298
            G   +I    TP+ D    +  W  R       DT        L A    +L +DR +L
Sbjct: 240 -GRHHIIYNCATPIDDGSIMLSQWLYRN------DTEEECSEAELIAWDAPILVEDREIL 292

Query: 299 ELMEPDA 305
           E  +PDA
Sbjct: 293 EATDPDA 299


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.139    0.450 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,105,604
Number of extensions: 47627
Number of successful extensions: 111
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 108
Number of HSP's successfully gapped: 2
Length of query: 349
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 263
Effective length of database: 1,212,660
Effective search space: 318929580
Effective search space used: 318929580
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 131 (55.1 bits)