BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0024.p (384 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding prote... 278 4e-76 gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-me... 119 4e-28 gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [He... 70 2e-13 gi|300311950|ref|YP_003776042.1| 2-polyprenyl-6-methoxyphenol hy... 62 8e-11 >gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 384 Score = 278 bits (712), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 144/359 (40%), Positives = 206/359 (57%), Gaps = 4/359 (1%) Query: 25 GHEVVVYEQSRQFLRVGADINLTPNAVRALDGLGIGEAVRRTAARPTHRISRTWNSGEET 84 G V VYEQ+ F R+GA I++ PN ++ L +GI + + R +RP SR W +G+ Sbjct: 27 GFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIGIEDEMNRQGSRPDFWYSRHWQTGDVL 86 Query: 85 SRLTMGDTAEQKYGAPQLTIHRADLLAALAEVFPLEQVRFAKRAEAIREEGDGIDLHFTD 144 +++ +GD A + YGA LT+HR D L + P E++ F K + + GD + L F D Sbjct: 87 AQIPLGDYAVKHYGASYLTVHRGDFHELLIKALPSERLSFGKCLTKVEDRGDVVILSFAD 146 Query: 145 GSTDRVDALIGGDGIHSAVRTAMFGAESPRFTGVVAFRAVVPAAKV-AGVPNLQAFTKWW 203 G+T+ D +IG DG++S +R + G E P++ G +A RAV P ++ AG+ A KWW Sbjct: 147 GTTEEADIVIGADGVNSRIREELLGVEPPKYAGYLAHRAVFPTPELKAGMLPFDACVKWW 206 Query: 204 GPNPESQIVTFPLNRGEDIFIFATTAQESWHLESWTTPGNVQELRDSYAGFHPDAMALLD 263 + +V F ++ +++ E W L P + +E+R+++ G+HP AL+D Sbjct: 207 SDD-RHMMVYFVTSKANELYYVTGVPVEKWDLNDRWLPSSKEEMRETFHGWHPTVQALVD 265 Query: 264 ACDEVLKTALYERDPLPAWARGNMALLGDACHPMMPFMAQGAGMAIEDAVVLARCLATVS 323 A EV K +L ERDPLP W+RG + LLGDACHPM P MAQGA MAIED +LARC V Sbjct: 266 ATVEVTKWSLLERDPLPLWSRGRLVLLGDACHPMKPHMAQGAAMAIEDGAMLARCFKEV- 324 Query: 324 ALGDVAAALHRYEQLRMERASTIQIGSRGNNWLRAGGNADWVYGYDAWQVPL-QPQVAA 381 + + A A YE R RAS +Q S N WLR + W +GYD + PL P AA Sbjct: 325 GVSNYADAFALYEANRAARASKVQRISHDNTWLRTNEDPSWCFGYDVFTEPLVSPSKAA 383 >gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 119 bits (298), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 104/355 (29%), Positives = 170/355 (47%), Gaps = 32/355 (9%) Query: 4 KIGVLGAGIGGLTAAIALQRAGHEVVVYEQSRQFLRVGADINLTPNAVRALDGLGIGEAV 63 +I ++G G G++AAI L+R G +V + E Q+ GA I+L P +RAL LG+ EA Sbjct: 7 RILIIGGGFSGMSAAIDLRRRGAQVDLVELDAQWRNYGAGISLGPATLRALKQLGVLEAF 66 Query: 64 RRTAARPTHRISRTWNSGEETSRLTMGDTAEQKYGAPQLTIHRADLLAALAEVFPLE--- 120 R A + G + + L + +P + A L LA + Sbjct: 67 LREGA-AGDGVRLCLPHGPQVAELPT-----PRLASPDVPGGGAILRPVLARILADATRA 120 Query: 121 ---QVRFAKRAEAIREEGDGIDLHFTDGSTDRVDALIGGDGIHSAVRTAMF-GAESPRFT 176 VR A+RE G +++ FTDG T R D +IG DG++S +R +F A PR++ Sbjct: 121 AGADVRLGCTFSAVREVGHKVEVDFTDGQTRRYDLVIGADGLYSKLRMHLFPHAPKPRYS 180 Query: 177 GVVAFRAVVPAAKVAGVPNLQAFTKWWGPNPESQIVTFPLNRGEDIFIFATTAQE-SWHL 235 G +RAV+P + + T W GP + + P+++ E +++F T + + H+ Sbjct: 181 GQAVWRAVLPRPQ-----EIVTCTMWMGPRIKPGV--NPVSKDE-MYLFVTEPRPVNEHV 232 Query: 236 ESWTTPGNVQELRDSYAGFHPDAMALLDACDEVLKTALYERD----PLPAWARGNMALLG 291 + T +++ L + ++ P + + D+ + + P P W +G + L+G Sbjct: 233 DPTTFVSHLRGLLEEFSA--PVLKTIREQLDDNARIIFRPLEGLLLPRP-WYQGRVVLIG 289 Query: 292 DACHPMMPFMAQGAGMAIEDAVVLARCLATVSALGDVAAALHRYEQLRMERASTI 346 DA H P +A GA + IEDA+VLA L G V AL +E+ R ER + Sbjct: 290 DAVHATTPHLASGACIGIEDALVLADEL---ERHGTVPQALAAFEERRWERCRMV 341 >gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 96/366 (26%), Positives = 135/366 (36%), Gaps = 43/366 (11%) Query: 3 FKIGVLGAGIGGLTAAIALQRAGHEVVVYEQSRQFLRVGADINLTPNAVRALDGLGIGEA 62 + + GAG GL A+ L+RAGH V + E+ Q VG+ + L P + L LG+ Sbjct: 4 LHVAIAGAGPAGLAMALYLKRAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGL--- 60 Query: 63 VRRTAARPTHRISRTWN----SGEETSRLTMGDTAEQKYGAPQLTIHRADLLAALAEVFP 118 R AA RI R + SG+ + A +YG + +HRA L L E Sbjct: 61 FSRIAAL-GQRIDRLYGADALSGKTVLDVRYDAIAGGRYG---IAVHRAALFGVLLEAAQ 116 Query: 119 LEQVRFAKRAEAIREEGDGIDLHFTDGSTDRV---DALIGGDGIHSAVRTAMFGAESPRF 175 E + + E G T R+ D +I G S +R + PR Sbjct: 117 QEGIEVETGQDIGNLEHTGAQACLTAADGRRIGGFDLVIDASGARSPLRRHALHSCEPRP 176 Query: 176 TGVVAFRAVV---PAAKVAGVPNLQAFTKWWGPNPESQIVTFPLNRGEDIFIFATTAQES 232 AF + P +Q + + + I P+ R T E Sbjct: 177 LPYGAFWVSLRAQPGLSFGHNSLVQRYRR-----ASTMIGVLPIGR-------PTPQDEP 224 Query: 233 WHLESWTT-PGNVQEL--------RDSYAGFHPDAMALLDACD--EVLKTALYERDPLPA 281 W+T P V+ L +D P+ L D E + A Y LP Sbjct: 225 MVAFFWSTRPDRVESLKQAGLSAWKDGVHALWPECAPYLAQIDGFEQMTLARYGHHTLPL 284 Query: 282 WARGNMALLGDACHPMMPFMAQGAGMAIEDAVVLARCLATVSALGDVAAALHRYEQLRME 341 +A++GDA H P + QGA MA+ DA L+ L L AL RY R Sbjct: 285 PYGERLAIIGDAAHSTSPQLGQGANMALLDARALSHALQQSETLEQ---ALPRYAASRRR 341 Query: 342 RASTIQ 347 T Q Sbjct: 342 HVRTFQ 347 >gi|300311950|ref|YP_003776042.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 407 Score = 62.0 bits (149), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 88/339 (25%), Positives = 139/339 (41%), Gaps = 44/339 (12%) Query: 21 LQRAGHEVVVYEQSRQFLRVGADINLT-PN--AVRALDGLG-IGEAVRRTAARPTHRISR 76 +QRAG V+V EQ+ + G + L+ P VR +D + +GE + +R R Sbjct: 29 MQRAGWRVLVVEQAEELRDGGHMMGLSGPGLATVRRMDLVARLGERAYPDMGQHIYRDRR 88 Query: 77 TWNSGEETSRLTMGDTAEQKYGAPQLTIHRADLLAALAEVFPLEQV--RFAKRAEAIREE 134 G E R+ + E G LT+ R L+ L P + RF + + Sbjct: 89 ----GREILRI---NYREALAGIEWLTLRRGVLVQELRAALPTRDIAWRFGVTVQEALNQ 141 Query: 135 GDGIDLHFTDGSTDRVDALIGGDGIHSAVRTAMFGAESPRF--TGVVAFRAVVPAAKVAG 192 + + +DGS D LI DG+HS++R F A++ G V + G Sbjct: 142 ASSVRVSLSDGSCHEADLLIAADGVHSSLRRRHFAADANCLHPLGYRYASYEVQDHLLLG 201 Query: 193 VPNLQAFTKWWGPNPESQIVTFPLNRGEDIFI-------FATTAQESWHLESWTTPGNVQ 245 Q F + P +++ R + + F AQ L + T Sbjct: 202 ----QDFLSYAEPGIQTEYYGLCDGRLAALHVWRSREAGFVPPAQRHSLLRTCTA----- 252 Query: 246 ELRDSYAGFHPDAMALLDACDEVL-KTALYERDPLPAWARGNMALLGDACHPMMPFMAQG 304 S+ G A+ + + V+ AL E LP+W +G + LLGDA H + QG Sbjct: 253 ---RSFTGVRATLEAVENPQEIVIDDLALVE---LPSWRQGRILLLGDAAHSLSLISGQG 306 Query: 305 AGMAIEDAVVLARCLATVSALG------DVAAALHRYEQ 337 AG+A+ A +LA L +A G + +AL R++Q Sbjct: 307 AGVALASASILAEELERAAAGGVAPSSSALESALARHDQ 345 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,160,105 Number of extensions: 48247 Number of successful extensions: 192 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 4 Length of query: 384 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 297 Effective length of database: 1,207,925 Effective search space: 358753725 Effective search space used: 358753725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)