BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0022.p (492 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 332 4e-92 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 326 3e-90 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 307 1e-84 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 295 6e-81 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 294 1e-80 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 332 bits (851), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 184/471 (39%), Positives = 270/471 (57%), Gaps = 15/471 (3%) Query: 18 PGAGDRYATLYPATGEAVASLHAASVADVEEAILGADRAFRTSGWAQKKPHERAAVLFRV 77 P G+ + + P G A + +S DVE A+ A A ++ W + P ERA +L ++ Sbjct: 30 PVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKS--WGKTSPTERANMLLKI 87 Query: 78 AQLIRENGEALAQLQRLDNGKPISETRNL-VASAAATFQFFAAACETLEESLTPMRGDNL 136 A + N E LA + LDNGKPI ET + A F++FAAA T E S+ P+ D Sbjct: 88 ADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSICPIDNDTY 147 Query: 137 TMSVHEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALALARLCIEA 196 HEP+GVV I PWN PI K+APALAAGN VV+KPAE TP + + L L + Sbjct: 148 AYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMVLIELIADL 207 Query: 197 GVPAGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTSTGKHIAHIAADKMMPVSLELGGK 256 +P G+++++ G G G + + + +++FTG T+TG+ I A+ ++PV+LELGGK Sbjct: 208 -IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGK 266 Query: 257 SPTMVFEDA------DIDHAVAGVLYGIFS-SSGESCIAGSRLFVARSLHEPFMERLAAG 309 SP + F D D A+ G + +F+ + GE C SR+ V S++E F+ER Sbjct: 267 SPNIFFADVLDKDDDFFDKALEG--FAMFALNQGEVCTCPSRVLVQESIYERFIERALKR 324 Query: 310 AAALRVGDPADERTQMGPLITARHREGIERYVDMGVTDGGRIRTGGVRPK-GPAFERGYF 368 AA++ G+P D+ T +G + E I Y+D+G +G ++ GG R + G GY+ Sbjct: 325 VAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREELGGDLASGYY 384 Query: 369 YTPTIIEGVRNDQRICQEEIFGPVLVAMPFDDEEDLIRQANDSVYALAAGIWTRDYKRAW 428 PT+ +G N RI QEEIFGPV+ F DEE+ + AND++Y L AG+WTRD RA+ Sbjct: 385 VKPTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAF 443 Query: 429 RVGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAM 479 R+GR +QAG VW N Y + FGG++ SG+GRE ++ + Y + K + Sbjct: 444 RMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNL 494 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 326 bits (835), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 170/411 (41%), Positives = 250/411 (60%), Gaps = 4/411 (0%) Query: 70 RAAVLFRVAQLIRENGEALAQLQRLDNGKPISETRNLVASAAATFQFFAAACETLEESLT 129 R L+ V +IR E LA+L+ + +GKPI + R + A F+++A + + Sbjct: 83 RGRALYAVGAVIRAEAEPLARLEAISSGKPIRDCRAEMQKVAEMFEYYAGWADKFYGEVI 142 Query: 130 PMRGDNLTMSVHEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALAL 189 P+ +L + EP G V +TPWN+P + ++ PALA GNAV++KP+E+TP ++LA+ Sbjct: 143 PVPSSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVLLKPSELTPFSSLAI 202 Query: 190 ARLCIEAGVPAGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTSTGKHIAHIAADKMMPV 249 ARL +AG+PAGL++VL G G + VK+V F G +TG IA AA ++MP Sbjct: 203 ARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGALIAKAAAARVMPC 262 Query: 250 SLELGGKSPTMVFEDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSLHEPFMERLAAG 309 LELGGKS ++FEDAD+ A G IFS +G+SC+AGSRL V R +++ F+E +AAG Sbjct: 263 VLELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYDRFVETVAAG 322 Query: 310 AAALRVGDPADERTQMGPLITARHREGIERYVDMGVTDGGRIRTGGVRPKGPAFERGYFY 369 A +R+G P D+ T++GP+ + E I+R V G+ G + G R E GYF Sbjct: 323 AEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHTRYG----EEGYFV 378 Query: 370 TPTIIEGVRNDQRICQEEIFGPVLVAMPFDDEEDLIRQANDSVYALAAGIWTRDYKRAWR 429 PT++ N + + EIFGPV VA+PF+DEE+ I ANDS + LA +WTRD RA R Sbjct: 379 RPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHR 438 Query: 430 VGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMY 480 V +V AG W+N+YK ++++PFGG+ SG GR G + Y + K+++ Sbjct: 439 VAASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSVW 489 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 307 bits (786), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 192/470 (40%), Positives = 263/470 (55%), Gaps = 10/470 (2%) Query: 12 IAGQWRPGAG-DRYATLYPATGEAVASLHAASVADVEEAILGADRAFRTSGWAQKKPHER 70 I G+W+P G +R A + PAT E V + + ADV++A+ A RA WA P ER Sbjct: 20 IDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAI--PAWAATSPLER 77 Query: 71 AAVLFRVAQLIRENGEALAQLQRLDNGKPISETRNLVASAAATFQFFAAACETLEESLTP 130 AA+L RV L+ E E AQ L+ G PIS R AA + A + L + Sbjct: 78 AALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAA--EHLRVARDNLAD-YPF 134 Query: 131 MRGDNLTMSVHEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALALA 190 +R T V EP+GV A ITPWN PI K+ PALAAG VV+KP+E++PL+AL A Sbjct: 135 IRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALLFA 194 Query: 191 RLCIEAGVPAGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTSTGKHIAHIAADKMMPVS 250 + EAGVPAG+ +++ G G+ +G A++ HP V +S TG T G +A AA + V+ Sbjct: 195 EIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRVA 254 Query: 251 LELGGKSPTMVFEDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSLHEPFMERLAAGA 310 ELGGKSP +V DAD++ A+ + + G+SC A +RL V R+ E A Sbjct: 255 QELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELALATL 314 Query: 311 AALRVGDPADERTQMGPLITARHREGIERYVDMGVTDGGRIRTGGV-RPKGPAFERGYFY 369 A ++VGDP D T GPL +++ ++ G+ DG R+ GG RP G +GY+ Sbjct: 315 AQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDG--LAQGYYA 372 Query: 370 TPTIIEGVRNDQRICQEEIFGPVLVAMPFDDEEDLIRQANDSVYALAAGIWTRDYKRAWR 429 PTI V D I QEEIFGPVL +P+D E+ I AND+VY L A + RD + Sbjct: 373 RPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRA 432 Query: 430 VGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAM 479 V +Q+G V +N Y + PFGG++ SG GRE G G+ +YME KA+ Sbjct: 433 VAARIQSGQVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 295 bits (755), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 183/465 (39%), Positives = 259/465 (55%), Gaps = 11/465 (2%) Query: 21 GDRYATLYPATGEAVASLHAASVADVEEAILGADRAFRTSGWAQKKPHERAAVLFRVAQL 80 G R + P E AS + ADV A+ A W++ +R +L ++A L Sbjct: 19 GQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSGLQRGQLLHKLAAL 78 Query: 81 IRENGEALAQLQRLDNGKPISETRNL-VASAAATFQFFAAACETLEESLTPMRG----DN 135 + + + LA + G+ E R + V +A + + A +E P G Sbjct: 79 VERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEGRTIPTAGYMGKPT 138 Query: 136 LTMSVHEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALALARLCIE 195 L+ +V EP+GVV AI PWN+P+ + K+A LAAG VV+KPAE TP +AL LARL E Sbjct: 139 LSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEETPQSALHLARLAQE 198 Query: 196 AGVPAGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTSTGKHIAHIAADKMMPVSLELGG 255 AG P G+I+V+ G G+ +G A+ HP V ++SFTG G+ I IA ++ V+LELGG Sbjct: 199 AGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVTLELGG 258 Query: 256 KSPTMVFEDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSLHEPFMERLAAGAAALRV 315 KSP +VF+DA + A+ G G+F + G+ C AGSR+ V RSL + F + LA AA + V Sbjct: 259 KSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGITV 318 Query: 316 GDPADERTQMGPLITARHREGIERYVDMGVTDGGRIRTGGV-RPKGPAFERGYFYTPTII 374 GDP+ +MGP+ E + RY+ G+ +G + GGV RP ++G+F PTI Sbjct: 319 GDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRP-----DKGWFVQPTIF 373 Query: 375 EGVRNDQRICQEEIFGPVLVAMPFDDEEDLIRQANDSVYALAAGIWTRDYKRAWRVGRAV 434 N I +EEIFGPV + FD EE+ I ANDS Y LAA +WT + RA RV V Sbjct: 374 ADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGV 433 Query: 435 QAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAM 479 + G V +N + + P+GG +DSG+GRE G G L Y E+K + Sbjct: 434 KVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTV 478 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 294 bits (753), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 172/463 (37%), Positives = 257/463 (55%), Gaps = 17/463 (3%) Query: 12 IAGQW-RPGAGDRYATLYPATGEAVASLHAASVADVEEAILGADRAFRTSGWAQKKPHER 70 IA +W P +G+ L P+ G+ ++ + D+++A+ A A+ + W + ER Sbjct: 6 IANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGA-WGRLSAAER 64 Query: 71 AAVLFRVAQLIRENGEALAQLQRLDNGKPISETRNLVASAAATFQFFAAACETLEESLTP 130 + +L ++A ++++ E LAQL+ D GKP+ + R A+ F+F+A A + L P Sbjct: 65 SRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHGDTIP 124 Query: 131 MRGDNLTMSVHEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALALA 190 + +++ EP GV I PWN P+ + + ALAAGN VVKPAE L+ L +A Sbjct: 125 YQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSILRVA 184 Query: 191 RLCIEAGVPAGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTSTGKHIAHIAADKMMPVS 250 L E G P G ++++ G G GD + HP V +SFTG + GK + AA+ PV+ Sbjct: 185 ELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHTPVT 244 Query: 251 LELGGKSPTMVFEDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSLHEPFMERLAAGA 310 LELGGKSP +VFEDAD++ + V+ I ++G++C AGSRL V R +E +ERL+ Sbjct: 245 LELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERLSGLF 304 Query: 311 AALRVGDPADERTQMGPLITARHREGIERYVDMGVTDG------GRIRTGGVRPKGPAFE 364 + L+VG + GPLI +E + ++ DG G+I G P G Sbjct: 305 SQLQVGS-SQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAG--VPTG---- 357 Query: 365 RGYFYTPTIIEGVRNDQRICQEEIFGPVLVAMPFDDEEDLIRQANDSVYALAAGIWTRDY 424 G++ P ++ V R+ QEE+FGPVL AMPF+DE I AN + Y L A +WTRD Sbjct: 358 -GFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDG 416 Query: 425 KRAWRVGRAVQAGNVWINTYKQ-FSISTPFGGWRDSGLGREKG 466 R R+ RA+++G V+IN Y + PFGG + SG GREKG Sbjct: 417 GRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKG 459 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,669,744 Number of extensions: 75737 Number of successful extensions: 326 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 265 Number of HSP's successfully gapped: 20 Length of query: 492 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 403 Effective length of database: 1,198,455 Effective search space: 482977365 Effective search space used: 482977365 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)