BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0020.p (599 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 119 5e-28 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 85 2e-17 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 79 1e-15 gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [H... 73 5e-14 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 119 bits (299), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 123/531 (23%), Positives = 231/531 (43%), Gaps = 36/531 (6%) Query: 26 LEQCGVKAAFGVISIHNMPILDAFGERGKIRFIPARGEAGGANMADAYARTTGGLGVCLT 85 L + GV+ FG + I DA ++ K + I R E + ADAY+R++ +GV + Sbjct: 16 LAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRSSQKVGVAIV 75 Query: 86 STGTAAGNAAGAMVEALTAGTPLLHLTGQIETPYLDQSLAYIHEAPDQLSMLKAISKAAF 145 ++G NA + A P++ ++GQ+ + + Q + D + + + K F Sbjct: 76 TSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDA---FQECDTVGITRPCVKHNF 132 Query: 146 RIRSVETAISTIKLAVQTALTPPMGPVSVEIPIDIQ----AAMIPLPINLQPLAIPQSVP 201 ++ V+ T+K A A T GPV V+IP DI A P + ++ P Sbjct: 133 LVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAVEMRSYK-PVDKG 191 Query: 202 SARALDELAERLMKAKRPLLWLGGGARHAGA--QVQRLL-ALGFGVVTSTQGRGIVPEDD 258 + + + + L+ A+RP+++ GGG A A ++ +L+ LG+ + G G Sbjct: 192 HSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMGLGAYRSSS 251 Query: 259 PRSLGAFNLHKPVE--QFYQSCDAMLVVGSRLR----GNETLKYELKLPRPLYRVDADPA 312 + +G +H E Q CD ++ +G+R GN K+ R + +D DP+ Sbjct: 252 DKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNP--KHFASHARKIIHIDIDPS 309 Query: 313 AEGRCYTSDYFVCGDSALVLAGLADRL---------AALKVDPDFMGDLKKARDTAVHGL 363 + + D + G+ VL L +L AAL + + +K RD Sbjct: 310 SISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRK-RDCLKFAT 368 Query: 364 VDGLGPYSALVEQLQQAAGREFNWVRDVTVSNSTWGNRHLRIFDPRAGVHALGGGIGQGL 423 + ++V+++ + + DV + W ++ PR +++ GG+G + Sbjct: 369 SEEFIKPQSVVQKVWEVTKGDAFITSDVG-QHQMWAAQYYGFDKPRRWINS--GGLGT-M 424 Query: 424 AMGIGAAIGAAVTQSGKKTFTLAGDGGFILNLGELATAVQEQADMVIVLMNDKGYGVIKN 483 +G+ A+G + + G+ + + ELAT Q I+L+N++ G+++ Sbjct: 425 GVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGMVRQ 484 Query: 484 IQDAQYGGR--RHYVDLHTPDYAELSSALKLRHKRVGDLAEVAAGLDEALS 532 Q YG R Y+D PD+ +L+ + ++ +V L EA + Sbjct: 485 WQQIDYGSRYSESYMD-SLPDFNKLAESYGHVGMKIEKPGDVDGALREAFA 534 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 84.7 bits (208), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 127/548 (23%), Positives = 224/548 (40%), Gaps = 35/548 (6%) Query: 9 MPNTQSKVTVGCAIAAFLEQCGVKAAFGVISIHNMPILDAFGERGKIRFIPARGEAGGAN 68 MP T + + + L + GV+ FGV+ P+ DA +I +I R E G A Sbjct: 1 MPATVADILIDT-----LYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAAL 55 Query: 69 MADAYARTTGGLGVCLTSTGTAAGNAAGAMVEALTAGTPLLHLTGQIETPYLDQSLAYIH 128 A A+ TG L VC +TG A + + EA P+L ++G + P + Y+ Sbjct: 56 AAAGQAKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGV--PAAHRGTDYLQ 113 Query: 129 EAPDQLSMLKAISKAAFRIRSVETAISTIKLAVQTALTPPMGPVSVEIPIDIQAAMIPLP 188 E +L + + ++ + I S + A + A+ A G + IP D+ A Sbjct: 114 ENSPEL-LFRDVAAYSQIITSPDQAPAVFHQAIAQAYA-QRGVAHLNIPADVIGAKTSGS 171 Query: 189 I-NLQPL--AIPQSVPSARALDELAERLMKAKRPLLWLGGGARHAGAQVQRLLA-LGFGV 244 + +L L P S P+A + A+ + A ++ G G R A +V L + L V Sbjct: 172 VPSLHTLRERTPLSPPAAD-IQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPV 230 Query: 245 VTSTQGRGIVPEDDPRSLGAFNL--HKPVEQFYQSCDAMLVVGSRLRGNETLKYELKLPR 302 + + +G+ + D P +G L P + + ML++GS +E L + K Sbjct: 231 MHTFRGKDLASYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSK--- 287 Query: 303 PLYRVDADPAAEGRCYTSDYFVCGDSALVLAGLADRLAALKVDPDFMGDLKKARD----- 357 + ++D GR + G A + L +R+ K D F+ R+ Sbjct: 288 -VIQIDERGFVLGRRAPVTLGITGSVAPAVQQLLERVEG-KTDGAFLQQANAHRERWNAK 345 Query: 358 ----TAVHGLVDGLGPYSALVEQLQQAAGREFNWVRDVTVSNSTWGNRHLRIFDPRAGVH 413 + + P S + + A + +V D V + W +R R G Sbjct: 346 LDSAAEIKRHHKKIKPQS-VARAISDLADDDAAFVVDTGVV-TLWCGNWIR---QRGGQR 400 Query: 414 ALGGGIGQGLAMGIGAAIGAAVTQSGKKTFTLAGDGGFILNLGELATAVQEQADMVIVLM 473 L + +G A G ++ GDGGF + LGE T+V+ + + +V+ Sbjct: 401 ILASFNNAAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVF 460 Query: 474 NDKGYGVIKNIQDAQYGGRRHYVDLHTPDYAELSSALKLRHKRVGDLAEVAAGLDEALSS 533 N++ +G++ + + PD+A + A + V E+ GL + L++ Sbjct: 461 NNREWGLVHLEMEEAGMPAFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAA 520 Query: 534 PGPFLLEI 541 PGP +L++ Sbjct: 521 PGPAVLDV 528 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 78.6 bits (192), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 129/550 (23%), Positives = 225/550 (40%), Gaps = 50/550 (9%) Query: 36 GVISIHNMPILDAFGE-----RGKIRFIPARGEAGGANMADAYART--TGGLGVCLTSTG 88 GV SI + GE R + A E A+ A AYA+ + TS G Sbjct: 40 GVFSIFGHGNVAGMGEALYQYRATLPTYRAHNEQAMAHSAIAYAKAHLRQRMMAVTTSIG 99 Query: 89 TAAGNAAGAMVEALTAGTPLLHLTGQI-----ETPYLDQSLAYIHEAPDQLSMLKAISKA 143 A N A A P+L L G + P L Q + + +S+ Sbjct: 100 PGATNLLTAAALAHVNRLPVLLLPGDVFVSRRPDPVLQQLEDFNDPGLSVNDAFRPLSRM 159 Query: 144 AFRIRSVETAISTIKLAVQTALTPPM-GPVSVEIPIDIQAAMIPLPINLQP--LAIPQS- 199 RI E ++ + A+Q P GPV++ +P D+Q P++ + +P++ Sbjct: 160 FDRICYPEQLLTALPRAIQVLTDPAQCGPVTLALPQDVQTMAYDYPLDFFSPRVIVPRAQ 219 Query: 200 VPSARALDELAERLMKAKRPLLWLGGGARHAGA-QVQRLLALGFGV-VTSTQ-GRGIVPE 256 P+A+ L++ L +A++PLL GGG + A + R A GV V TQ G+ +P Sbjct: 220 APAAQELEDAVALLRQARQPLLIAGGGVLYGNACEHLRRFAEQHGVPVAETQAGKSALPW 279 Query: 257 DDPRSLGAFNL--HKPVEQFYQSCDAMLVVGSRLR----GNETLKYELKLPRPLYRVDAD 310 +GA + Q D ++ VG+RL+ G+ TL + +L V+A Sbjct: 280 SHALQMGAIGVTGSPAANALAQEADVVIAVGTRLQDFTTGSHTLFAQARLLN--LNVNAM 337 Query: 311 PAAEGRCYTSDYFVCGDSALVLAGLADRLAALKVDPDF------MGDLKKARDTAVHGLV 364 A + R + D+ L L L+ L + P++ + + R + G Sbjct: 338 DAHKWRGLA----LQADAGLGLEALSHALEGWRSAPEWHARAHTLAQDWRERVAQITGQT 393 Query: 365 DGLG--PYSA-LVEQLQQAAGREFNWVRDVTVSNSTWGNRHLRIFDPRAGVHALGGGIGQ 421 D G PY ++ +Q++ + V + + H R G + + G Sbjct: 394 DTGGRLPYDGEVIGAIQRSVADSPSQDIVVCAAGTLPAELHKLWRAGRPGAYHVEYGYS- 452 Query: 422 GLAMGIGAAIGAAVTQSGKKTFTLAGDGGFILNLGELATAVQEQADMVIVLMNDKGYGVI 481 + + +G + Q ++ + GDG +++ ELAT+V A +++V+++++GYG I Sbjct: 453 CMGYEVAGGLGVKLAQPQREVIVIVGDGSYLMMNSELATSVMLGAKLIVVVLDNRGYGCI 512 Query: 482 KNIQDAQYGG--RRHYVDLHTP-------DYAELSSALKLRHKRVGDLAEVAAGLDEALS 532 +Q A G D D+A + +L + V + E+ A L A + Sbjct: 513 NRLQQACGGAPFNNMLADCLQAGPGAPAIDFAAHARSLGALGENVKTITELEAALQRARA 572 Query: 533 SPGPFLLEID 542 + +L+ ID Sbjct: 573 ADRSYLVCID 582 >gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 73.2 bits (178), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 139/566 (24%), Positives = 220/566 (38%), Gaps = 63/566 (11%) Query: 2 FQEDFPAMPNTQSKVTVGCAIAAFLEQCGVKAAFGVISIHNMPILDAFGERGKIRFIPAR 61 Q+ FP +TV A+ + + G+ + F +P+ F E R++ Sbjct: 1 MQQKFP------HPITVRHAVLDLMRRLGMTSVFANPGSTELPMFRDFPE--DFRYVLGL 52 Query: 62 GEAGGANMADAYARTTGGLGVCLTSTGTAAGNAAGAMVEALTAGTPLLHLTGQIETPYL- 120 EA MAD +A+ +G + + GNA G + A TPL+ GQ L Sbjct: 53 QEAVVVGMADGFAQASGNAALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILP 112 Query: 121 -DQSLAYIHEAPDQLSMLKAISKAAFRIRSVETAISTIKLAVQTALTPPMGPVSVEIPID 179 D L LK + A R+ + ++ + A A+ P GPV V IP+D Sbjct: 113 FDPFLGATQATELAKPYLKWSIEPA---RAADVPLALAR-AYHMAMQEPRGPVFVSIPVD 168 Query: 180 I--QAAMIPLPINLQPLAIPQSVPSARALDELAERLMKAKRPLLWLGGGARHAGA----- 232 Q A + P + + P A+ LA L +++RP + +G AGA Sbjct: 169 DWDQPAELVPPTQV----TSRCRPEPAAIARLAAALEQSQRPAIVVGAELDRAGAWDAAI 224 Query: 233 -QVQRLLALGFGVVTSTQGRGIVPEDDPRSLGAF-NLHKPVEQFYQSCDAMLVVGSRLRG 290 + L A F V R PED P G + + + Q Q D + +G+ Sbjct: 225 ALAETLRARVF--VAPMSSRCSFPEDHPLFAGFLPAMREKIVQALQGHDLLFALGA---- 278 Query: 291 NETLKYELKLPRP-------LYRVDADPAAEGRCYTSDYFVCGDSALVLAGLADRLAALK 343 Y ++ P L+++ DP T V G+ L GL D LA ++ Sbjct: 279 -PAFTYHVEGSGPHVPPGAALFQLIDDPVTASWTPTGQAIV-GNIQL---GLDDLLAQIR 333 Query: 344 VDPDFMGDLKKARDTAVHGLVDGLGPYSALVEQLQQAAGREFNWVRDVTVSNSTWGNR-- 401 P AR V+ P S + LQ A + D+ V + G+R Sbjct: 334 RQPPTQRAQPPARPA--RARVEASAPLS-MAYALQTLA--DVREAGDIVVEEAP-GSRPV 387 Query: 402 ---HLRIFDPRAGVHALGGGIGQGLAMGIGAAIGAAVTQSGKKTFTLAGDGGFILNLGEL 458 HL P + GG+G G+ AA+G A+ + + L GDG + + + Sbjct: 388 MQAHLPFTRPGSFYTMASGGLGYGM----PAAVGVALARPHSRVIGLIGDGSAMYSPQAI 443 Query: 459 ATAVQEQADMVIVLMNDKGYGVIKNIQDAQYGGRR---HYVDLHTPDYAELSSALKLRHK 515 +AVQ + V++N+ Y +++ G H +L D+ L+ ++ Sbjct: 444 YSAVQLGLPITFVILNNGRYAALQDFAPVFGFGPEDPVHGTELPGLDFVALAHSMGCHGV 503 Query: 516 RVGDLAEVAAGLDEALSSPGPFLLEI 541 RV A +A L EAL S L+EI Sbjct: 504 RVSQAAALAPALREALCSNETRLVEI 529 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,869,258 Number of extensions: 79511 Number of successful extensions: 282 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 4 Length of query: 599 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 508 Effective length of database: 1,188,985 Effective search space: 604004380 Effective search space used: 604004380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)