BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0014.p (286 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate A... 133 2e-32 gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate A... 113 1e-26 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 107 8e-25 gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate A... 106 3e-24 gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate A... 91 1e-19 >gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 283 Score = 133 bits (335), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 75/204 (36%), Positives = 114/204 (55%) Query: 81 AQLLQHFGITAMEVVFGFLFGALLGLAVGVALGLSPRAEAMLSPYILALQIAPKVAFAPL 140 A++ H IT E V F+ G + GLA+G+ L LSP A A+L PY+ A P+V AP+ Sbjct: 77 AEIYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDPYVKAANSMPRVILAPI 136 Query: 141 FVMWLGYTIYPKIIVAVLIVFFPIMINVLSAIRTVDPDMVNLVRTLNAGRWQIFHLVEFR 200 F MW G I+ K+ +AV +VFF + NV ++ V P ++ R L A Q+ V Sbjct: 137 FAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANARQLLRTVYLP 196 Query: 201 SALPALFSGLRIASTLSVIGVTVGELVGGNQGLGYLLVDGEGQGNTAAVFVSIVALTLIG 260 SA +FS L + L+ +G VGE +G +G+GYL++ EG + VF IV LT+ Sbjct: 197 SATSWVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTFDINTVFAGIVVLTIFA 256 Query: 261 VVTYGAVVWAERKVLHYLPKANTS 284 +V AV E++++ + PK+ + Sbjct: 257 LVLDLAVGVIEKRLMKWQPKSGET 280 >gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 259 Score = 113 bits (283), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 70/243 (28%), Positives = 122/243 (50%), Gaps = 2/243 (0%) Query: 31 DKAQTVCGSLVLLVLFIAAWQWGPGMLGMPEYILPNFMQVVREGVVMWNNAQLLQHFGIT 90 ++A T LL++ + WQ+ + + ++I P+ +++ V + +H T Sbjct: 10 NRAATTLAPWFLLLVILGVWQFICSVWQVSDFIFPSPGSILQAMVD--YAGPITEHALST 67 Query: 91 AMEVVFGFLFGALLGLAVGVALGLSPRAEAMLSPYILALQIAPKVAFAPLFVMWLGYTIY 150 + GF ++G+ +G +G SP A P + A PK AF P+ V+W G Sbjct: 68 FWVTMVGFAIAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVVWFGIGAG 127 Query: 151 PKIIVAVLIVFFPIMINVLSAIRTVDPDMVNLVRTLNAGRWQIFHLVEFRSALPALFSGL 210 P I+ A LI FFPIM+N+ + + T++P++ +++R L A R I V ++P F+ L Sbjct: 128 PAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYFFASL 187 Query: 211 RIASTLSVIGVTVGELVGGNQGLGYLLVDGEGQGNTAAVFVSIVALTLIGVVTYGAVVWA 270 ++A TL+ +G TV E+ NQG+GYLLV F +V + + + Y Sbjct: 188 KVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGAMAMAMYELFAII 247 Query: 271 ERK 273 E++ Sbjct: 248 EKR 250 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 107 bits (268), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 67/227 (29%), Positives = 121/227 (53%) Query: 59 MPEYILPNFMQVVREGVVMWNNAQLLQHFGITAMEVVFGFLFGALLGLAVGVALGLSPRA 118 +P +LP ++ + +LL H + V GF+ G+ L +A+G+ +G+S RA Sbjct: 48 IPARLLPPPSELAHTLWSLAQRGELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRA 107 Query: 119 EAMLSPYILALQIAPKVAFAPLFVMWLGYTIYPKIIVAVLIVFFPIMINVLSAIRTVDPD 178 EA+L P AL+ P +A+ PL ++WLG PK+++ + FFP+ +N+++ IR +D Sbjct: 108 EALLEPSFQALRAIPSLAWIPLLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHK 167 Query: 179 MVNLVRTLNAGRWQIFHLVEFRSALPALFSGLRIASTLSVIGVTVGELVGGNQGLGYLLV 238 ++ + T + + ++LP LF+GLR +L+ + + EL+ +G+GYLL Sbjct: 168 LIEVGTTSGLNTQALIGHILLPASLPYLFAGLRGGLSLAWMFLVAAELIAATKGVGYLLS 227 Query: 239 DGEGQGNTAAVFVSIVALTLIGVVTYGAVVWAERKVLHYLPKANTST 285 DG V +I+ L L+G ++ G + E+K L + S+ Sbjct: 228 DGRESSRPDLVLAAILILALLGKLSDGLLRAVEQKSLGWREARRQSS 274 >gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 267 Score = 106 bits (264), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 69/221 (31%), Positives = 107/221 (48%), Gaps = 6/221 (2%) Query: 36 VCGSLVLLVLFIAAWQWGPGMLGMPEYILPNFMQVVREGVVMWNNAQLLQHFGITAMEVV 95 V L++ + + WQ +L +P Y++P +V+ W+ L+ +T Sbjct: 17 VAAPLIIGIALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWS--MLMASLLVTLKITF 74 Query: 96 FGFLFGALLGLAVGVALGLSPRAEAMLSPYILALQIAPKVAFAPLFVMWLGYTIYPKIIV 155 F +LG+A+ S E L PY + LQ+ P VA APL ++W+ T + Sbjct: 75 LAFALAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLIIIWVKDTTAALTVC 134 Query: 156 AVLIVFFPIMINVLSAIRTVDPDMVNLVRTLNAGRWQIFHLVEFRSALPALFSGLRIAST 215 A ++ FPI+ N +R+V+P ++NL R A RWQ + SALP F GLRI+S Sbjct: 135 ATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSALPYFFGGLRISSG 194 Query: 216 LSVIGVTVGELVGGN----QGLGYLLVDGEGQGNTAAVFVS 252 L++IG V E V G GL Y ++ Q N +F + Sbjct: 195 LALIGAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAA 235 >gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 284 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 73/285 (25%), Positives = 132/285 (46%), Gaps = 10/285 (3%) Query: 4 TTAKDLTMSSSEPSAAAPSRPRRHFLSDKAQTVCGSLVLLVLFIAAWQ------W-GPGM 56 T A+ +++ AA + +R + K + + +LV+ + W+ W P Sbjct: 2 TQAQASQSAATMDIAAVEAEAQRKIRARKQLVIGLRIAILVIVLGGWEVCARIGWIDPFF 61 Query: 57 LGMPEYILPNFMQVVREGVVMWNNAQLLQHFGITAMEVVFGFLFGALLGLAVGVALGLSP 116 P I+ + EG + L +T E V GFL G++ G+ G+ LG + Sbjct: 62 FSQPSLIVAQIYDWMVEGT---SQGPLWTQVLVTLEETVLGFLIGSVAGVIAGIVLGRNK 118 Query: 117 RAEAMLSPYILALQIAPKVAFAPLFVMWLGYTIYPKIIVAVLIVFFPIMINVLSAIRTVD 176 + S YI P+V +FV+ G + K+ +AV++VFF + N +R D Sbjct: 119 LLSDVFSLYIQIANSIPRVVLGSIFVIAFGLGMASKVALAVVMVFFVVFANAFQGVREAD 178 Query: 177 PDMVNLVRTLNAGRWQIFHLVEFRSALPALFSGLRIASTLSVIGVTVGELVGGNQGLGYL 236 M+ + L A R Q+ V SAL + + L ++ +++G VGE +G QG+G L Sbjct: 179 RYMIANAQILGASRRQVTMAVVIPSALSWILASLHVSFGFALVGAVVGEFLGSKQGIGLL 238 Query: 237 LVDGEGQGNTAAVFVSIVALTLIGVVTYGAVVWAERKVLHYLPKA 281 + +G N + VF +++ L ++ + + ER++L + P+A Sbjct: 239 ISTAQGAFNASGVFAAMIVLAVVALAADWLLHALERRLLKWRPQA 283 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 781,455 Number of extensions: 30575 Number of successful extensions: 175 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 172 Number of HSP's successfully gapped: 14 Length of query: 286 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 202 Effective length of database: 1,222,130 Effective search space: 246870260 Effective search space used: 246870260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)