BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0011.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis tra... 625 e-180 gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis tra... 308 4e-85 gi|300309796|ref|YP_003773888.1| methyl-accepting chemotaxis pro... 260 1e-70 gi|300313926|ref|YP_003778018.1| methyl-accepting chemotaxis tra... 225 4e-60 gi|300313925|ref|YP_003778017.1| methyl-accepting chemotaxis sen... 220 1e-58 >gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 625 bits (1613), Expect = e-180, Method: Compositional matrix adjust. Identities = 321/378 (84%), Positives = 352/378 (93%) Query: 1 MNKLKVSTRLALGFAGVIVLLLVISLVGMWGLSTVKSNLSNIVANNQQGGLADVMNNSVR 60 MNKLKVSTRLALGFA V+VLL+VISLVG+WG++TVK NLS+IVANNQQGGLADVMNNSVR Sbjct: 1 MNKLKVSTRLALGFASVVVLLVVISLVGLWGMATVKGNLSDIVANNQQGGLADVMNNSVR 60 Query: 61 IRALAIRNIALFQDPALVSAEIERIKVQEKSYQEARDKLRKLFDAPDTSRKEREFFDQIA 120 IRALAIRNIALFQ+PAL++ EIERIKVQEK+YQ+AR+KL K+FD PDT+R+E E FD IA Sbjct: 61 IRALAIRNIALFQEPALIAREIERIKVQEKAYQDAREKLGKMFDQPDTTRREHELFDLIA 120 Query: 121 QNAADTEPLLKQAVTLAQENKTTDLINLLVQKIRPVQAKWLDNLDALGRLEDDLNDQAAA 180 QNAADTEPL+ QAVTLAQ++K+ +LI+LL QKIRPVQAKWLDNLD LGR ED+LN+QA+A Sbjct: 121 QNAADTEPLMNQAVTLAQQDKSAELISLLTQKIRPVQAKWLDNLDELGRFEDELNNQASA 180 Query: 181 DAETTYQRIATTIWTLVAGSVLLALGAAVLIVRSVLRQLGGEPADAQVLAAQIAQGNLAV 240 A+TTY+ I TT WTLVAGSVLLA+ AA LIVRSVL QLGGEPA AQ LAAQIAQGNLAV Sbjct: 181 AADTTYRSILTTTWTLVAGSVLLAVAAAFLIVRSVLHQLGGEPAQAQELAAQIAQGNLAV 240 Query: 241 SIPLAKGDQHSLMASLDNMRAQLTSIVDGIKRSAESISTAAGQIAVGNLDLSRRTEEQAA 300 SIPLAKGDQHSLMASL+NMRAQLT IVDGIK SAESISTAA QIAVGNLDLSRRTEEQAA Sbjct: 241 SIPLAKGDQHSLMASLENMRAQLTRIVDGIKHSAESISTAASQIAVGNLDLSRRTEEQAA 300 Query: 301 SLEETASSMEEITSTVEQNTQNAGQGNQLARAASLHAQKGGEVVERVVDTMKDISQSAYK 360 SLEETASSMEEITSTVEQNTQNAGQG+QLAR+ASLHAQKGGEVVERVV TMKDISQSAYK Sbjct: 301 SLEETASSMEEITSTVEQNTQNAGQGHQLARSASLHAQKGGEVVERVVGTMKDISQSAYK 360 Query: 361 ITEIISTVEGIAFQTNIL 378 ITEII+TVEGIAFQTNIL Sbjct: 361 ITEIIATVEGIAFQTNIL 378 >gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 547 Score = 308 bits (790), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 176/380 (46%), Positives = 246/380 (64%), Gaps = 2/380 (0%) Query: 1 MNKLKVSTRLALGFAGVIVLLLVISLVGMWGLSTVKSNLSNIV-ANNQQGGLADVMNNSV 59 M LKV TRLALGF ++V LL IS +G++G++ V L++I NN + L + N++ Sbjct: 1 MGNLKVVTRLALGFGLILVFLLSISALGLYGMARVNDALTDITDVNNAEIKLVTALRNAL 60 Query: 60 RIRALAIRNIALFQDPALVSAEIERIKVQEKSYQEARDKLRKLFDA-PDTSRKEREFFDQ 118 RALAIRNIAL + A + E + + QEK Y +A L K+F T+ +ER+ Q Sbjct: 61 SQRALAIRNIALLDEQADMQVESQTLARQEKLYADAYAALDKMFTTEAATTERERQLLVQ 120 Query: 119 IAQNAADTEPLLKQAVTLAQENKTTDLINLLVQKIRPVQAKWLDNLDALGRLEDDLNDQA 178 I + A T PL+ +AV L ENK + +L+ ++RP Q W+ +L L ED LND+A Sbjct: 121 IKNDEAATLPLMAKAVQLGLENKAAEAGKMLITQVRPKQQAWIKSLTELAEFEDQLNDEA 180 Query: 179 AADAETTYQRIATTIWTLVAGSVLLALGAAVLIVRSVLRQLGGEPADAQVLAAQIAQGNL 238 A A TY + ++L+ + A+ LI RS+LRQLG EP++AQ +A IA G+L Sbjct: 181 ATSARNTYAWLRMVTVAAALAALLVGVAASALIARSILRQLGAEPSEAQAVARDIASGDL 240 Query: 239 AVSIPLAKGDQHSLMASLDNMRAQLTSIVDGIKRSAESISTAAGQIAVGNLDLSRRTEEQ 298 ++ + +GD SLMAS++ MR QL I GIK +AE+IS A+GQIA GN DLS+RTEEQ Sbjct: 241 TATVRVREGDSSSLMASIEQMRYQLNVIAHGIKSAAETISVASGQIAQGNFDLSQRTEEQ 300 Query: 299 AASLEETASSMEEITSTVEQNTQNAGQGNQLARAASLHAQKGGEVVERVVDTMKDISQSA 358 AASLEETA+SMEE+T+TV QNTQ+A + +L+ A+ A G + +RVV TM I+ S+ Sbjct: 301 AASLEETAASMEELTTTVRQNTQHASEALRLSGGATETAAAGSQAFDRVVQTMDRITASS 360 Query: 359 YKITEIISTVEGIAFQTNIL 378 K+++IIS +EGIAFQTNIL Sbjct: 361 SKMSDIISVIEGIAFQTNIL 380 >gi|300309796|ref|YP_003773888.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 260 bits (665), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 158/377 (41%), Positives = 239/377 (63%), Gaps = 2/377 (0%) Query: 4 LKVSTRLALGFAGVIVLLLVISLVGMWGLSTVKSNLSNIV-ANNQQGGLADVMNNSVRIR 62 +K+ TRL LGFA V++LL++++ G++ + V+ +++V N + L M +V R Sbjct: 5 MKMGTRLGLGFAWVVLLLVLVTGFGIFNMHQVQGRFTHVVEVKNPESALVKEMLETVGER 64 Query: 63 ALAIRNIALFQDPALVSAEIERIKVQEKSYQEARDKLRKLF-DAPDTSRKEREFFDQIAQ 121 ++++RN+ L P V E + I+ Q Y+ A KL++LF ++ DTS +E+ +IA Sbjct: 65 SISLRNLMLPAAPEDVDIEAQYIEAQSLKYKMAAQKLQQLFAESSDTSDEEKAALPKIAA 124 Query: 122 NAADTEPLLKQAVTLAQENKTTDLINLLVQKIRPVQAKWLDNLDALGRLEDDLNDQAAAD 181 A+ E L+ +AV + + +L L ++ PVQ W L AL LED LN +AA Sbjct: 125 QASKAEKLINEAVEFGKRREAYELAQFLKEQYLPVQKSWQVELKALAALEDQLNKEAAEA 184 Query: 182 AETTYQRIATTIWTLVAGSVLLALGAAVLIVRSVLRQLGGEPADAQVLAAQIAQGNLAVS 241 + Y R + + A V+ A+ AA + R +LR+LGGEP A +A QIA+G+LAV Sbjct: 185 SAVAYSRARMLMLLISAIGVVTAVLAAFWLTRHLLRKLGGEPDYAVQIAGQIAEGDLAVV 244 Query: 242 IPLAKGDQHSLMASLDNMRAQLTSIVDGIKRSAESISTAAGQIAVGNLDLSRRTEEQAAS 301 I GD SLMA++ MR +L +IV +++ +E+I+TA+ +IA GNLDLS RTE+QA + Sbjct: 245 IDTRAGDHDSLMAAMRVMRDKLAAIVAEVRKGSETITTASSEIARGNLDLSGRTEQQAGA 304 Query: 302 LEETASSMEEITSTVEQNTQNAGQGNQLARAASLHAQKGGEVVERVVDTMKDISQSAYKI 361 LEETASSME++ STV+QN +NA Q N LA AAS A +GG +V +VV TM+ I+ S+ K+ Sbjct: 305 LEETASSMEQLNSTVKQNAENAHQANDLALAASDVAVQGGSIVSQVVQTMQSINASSQKV 364 Query: 362 TEIISTVEGIAFQTNIL 378 +I+S ++GIAFQTNIL Sbjct: 365 ADIVSVIDGIAFQTNIL 381 >gi|300313926|ref|YP_003778018.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 582 Score = 225 bits (574), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 164/402 (40%), Positives = 221/402 (54%), Gaps = 56/402 (13%) Query: 4 LKVSTRLALGFAGVIVLLLVISLVGMWGLSTVKSNLSNIVANNQQGGLADVM-----NNS 58 + V +L L F V+V+ LV S + + + ++ + + Q G AD M N Sbjct: 5 MTVGRKLGLAFLCVVVVALVGSSLSLLNFNRLEDASAMNIHTYQVMGKADDMLLNMVNIE 64 Query: 59 VRIRALAIRNIALFQDPALVSAEIERIKVQEKSYQEARDKLRKLF-DAPDTSRKEREF-- 115 IR F DP K K ++ A D + L D P ++ E Sbjct: 65 TGIRGYVASGNEAFLDP---------YKGGRKDFKVAFDDAKSLTADNPVQQKRLAEMQE 115 Query: 116 ----FDQIAQN---------AADTEPLLKQAVTLAQENKTTDLINLLVQKIRPVQAKWLD 162 ++I QN A+ ++ L Q A +K + + + R VQ +++D Sbjct: 116 LSNKIEEIDQNLIKLRKEANASGSQQAL-QTYFGATHDK------IFMDRFRVVQKEFVD 168 Query: 163 NLDAL----GRLEDDLNDQAAADAETTYQRIATTIWTLVAG--SVLLALGAAVLIVRSVL 216 ++L G L L+ +T I + AG +VLLA+GA +LI RS++ Sbjct: 169 AEESLLKERGELVRSLSK-------------STLIVMIAAGALTVLLAIGAGLLITRSII 215 Query: 217 RQLGGEPADAQVLAAQIAQGNLAVSIPLAKGDQHSLMASLDNMRAQLTSIVDGIKRSAES 276 LGGEP A +A QIA G+L V++PL D HSLMASL +MR QLT+IV GI+ S ES Sbjct: 216 NALGGEPGAAADVAQQIAAGDLTVAVPLKPNDSHSLMASLGSMREQLTTIVRGIQTSGES 275 Query: 277 ISTAAGQIAVGNLDLSRRTEEQAASLEETASSMEEITSTVEQNTQNAGQGNQLARAASLH 336 IS AA +IA GN DLS+RTEEQAASLEETASSMEE+TSTV QN+ NA Q N LA AS Sbjct: 276 ISVAANEIATGNTDLSQRTEEQAASLEETASSMEELTSTVRQNSDNARQANTLAENASTL 335 Query: 337 AQKGGEVVERVVDTMKDISQSAYKITEIISTVEGIAFQTNIL 378 A KGGEVV RV+DTM +IS S+ K+++II+ +EGIAFQTNIL Sbjct: 336 AAKGGEVVSRVIDTMNEISDSSSKVSDIITVIEGIAFQTNIL 377 >gi|300313925|ref|YP_003778017.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 522 Score = 220 bits (561), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 150/380 (39%), Positives = 216/380 (56%), Gaps = 2/380 (0%) Query: 1 MNKLKVSTRLALGFAGVIVLLLVISLVGMWGLSTVKSNLSNIV-ANNQQGGLADVMNNSV 59 +L VS+RL GF V+ L+L+I + + L+ V L +I NN + M + Sbjct: 3 FTRLTVSSRLKFGFGVVLFLILLIVGIALKKLAEVNRQLVSITEVNNVEIYHLSTMRAAA 62 Query: 60 RIRALAIRNIALFQDPALVSAEIERIKVQEKSYQEARDKLRKLF-DAPDTSRKEREFFDQ 118 ++LA R I L P ++ ++ Q Y ++ L KLF D +T+ E++ Sbjct: 63 YEQSLASRGIGLALTPEDLNQNANNLQRQIGLYTQSEAALAKLFADLQETTDTEKQALAA 122 Query: 119 IAQNAADTEPLLKQAVTLAQENKTTDLINLLVQKIRPVQAKWLDNLDALGRLEDDLNDQA 178 I + +A P L + LA+ KT +L ++ + +QA L L ED LN++A Sbjct: 123 IQKMSAGLSPGLSNMIDLAKAQKTEELKPIMTGPLGKMQADRRKALAELSAFEDKLNNEA 182 Query: 179 AADAETTYQRIATTIWTLVAGSVLLALGAAVLIVRSVLRQLGGEPADAQVLAAQIAQGNL 238 +A + L ++L L AAVLI RSVLRQLGGEPA A LAAQIA+G+L Sbjct: 183 KDEALAVSASARKLLIALGVIALLCGLMAAVLITRSVLRQLGGEPAYASRLAAQIAKGDL 242 Query: 239 AVSIPLAKGDQHSLMASLDNMRAQLTSIVDGIKRSAESISTAAGQIAVGNLDLSRRTEEQ 298 + SLM S+ M L IV ++ ++I+TA+ QIA GNLDLS RTEEQ Sbjct: 243 RTEVKADTRHPESLMMSMKTMNEGLRGIVREVRSGTDAIATASSQIASGNLDLSSRTEEQ 302 Query: 299 AASLEETASSMEEITSTVEQNTQNAGQGNQLARAASLHAQKGGEVVERVVDTMKDISQSA 358 A+SLEETAS+ME++T+TV+QN +AG+ N+LA AS A + G++V+ VV TM I S+ Sbjct: 303 ASSLEETASAMEQLTATVKQNADSAGEANRLAAEASAVASQSGQLVKEVVTTMAAIEDSS 362 Query: 359 YKITEIISTVEGIAFQTNIL 378 KI +IIS ++GI+FQTNIL Sbjct: 363 KKIVDIISVIDGISFQTNIL 382 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.127 0.339 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 910,305 Number of extensions: 31027 Number of successful extensions: 460 Number of sequences better than 1.0e-08: 65 Number of HSP's gapped: 377 Number of HSP's successfully gapped: 65 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)