BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0005.p (615 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311601|ref|YP_003775693.1| glutamine ABC transporter perip... 103 4e-23 gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signa... 87 5e-18 gi|300313535|ref|YP_003777627.1| amino acid ABC transporter/sign... 84 2e-17 gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/sign... 84 2e-17 gi|300313169|ref|YP_003777261.1| amino acid transport periplasmi... 77 3e-15 >gi|300311601|ref|YP_003775693.1| glutamine ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 251 Score = 103 bits (257), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 72/238 (30%), Positives = 116/238 (48%), Gaps = 6/238 (2%) Query: 347 SASALLSA--GAMAQGGPLRTGVDATFAPHAMVKLGGGLQGFNIDLGEELARRLGRKIEI 404 S S L++A A A G +R G DATF P VK G GF+++L E LA+ +G+K++ Sbjct: 14 SLSLLIAAVPAAHANGEVIRVGTDATFPPMEFVK-DGKRTGFDVELIEALAKTMGKKVQW 72 Query: 405 EGTEFSGLVPGLNAKKYDFVLAPVTATPERAKAMLFTEGYLDTDYTFVIKKGAAPINALD 464 +F GL+PGL + ++D + + T ER K + F++ Y ++++ I + Sbjct: 73 IDIDFKGLIPGLVSNRFDIAASAIYMTDERRKVVNFSDPYYRGGLAVLVRRDDNSIKVPE 132 Query: 465 DL-KGKTISVNKGSAYELWARDNAPKYGFKYDVYGNNADAVQAVQSGRADANLAGSTVAA 523 DL KGK +SV G+ + +DN P G + N + +GR +A + G A Sbjct: 133 DLNKGKRVSVQVGTKSVGYLKDNFP--GVERVEVEKNQAMFDLLATGRVNAVVTGRPAAV 190 Query: 524 WAAKQNPAVQTTYTVKTGLVWALAFRLDDKVGRDAAGMALKCMKQDGTLGKIAQKWFG 581 A+ P V+ T ++ A R D AL+ ++ +GT + KWFG Sbjct: 191 EYARTQPLVRVLDKGLTTELYGFALRKSDTELTAQLNKALQTLRMNGTYTALTDKWFG 248 >gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 86.7 bits (213), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 73/253 (28%), Positives = 121/253 (47%), Gaps = 15/253 (5%) Query: 338 LKYSLLAVLSASALLSAGAMAQGGPLRTGVDATFAPHAMVKLGGGLQGFNIDLGEELARR 397 L ++ L S AL GA A+ L D F P K GF+IDL + +A+ Sbjct: 3 LPFAKLVFASLVALSVVGASARAETLVVACDTAFVPFEF-KDRNQYVGFDIDLWDAIAKD 61 Query: 398 LGRKIEIEGTEFSGLVPGLNAKKYDFVLAPVTATPERAKAMLFTEGYLDTDYTFVIKKGA 457 +G +++ +F+G++P L K D LA +T ER K + F++GY D+ ++ ++ G+ Sbjct: 62 IGVTYKLQPMDFNGIIPALQTKNVDVALAGITIKDERKKVIDFSDGYYDSGFSLMVPVGS 121 Query: 458 APINALDDLKGKTISVNKGSAYELWARDNAPKYGFKYDVYGNNADAVQAVQSGRADANLA 517 + I ++ DL GK ++V G++ +A+ N + + N +A +Q+GR DA + Sbjct: 122 S-IKSVADLAGKALAVKSGTSAFDYAKANFK--ATELHQFPNIDNAYLELQTGRVDAAMH 178 Query: 518 GST----VAAWAAKQNPAVQTTYTV--KTGLVWALAFRLDDKVGRDAAGMALKCMKQDGT 571 + A A K V T + + G+ + L KV AL +K DG Sbjct: 179 DTPNVLYYIATAGKGRAKVVGTQMMAHQYGIGFPKGSPLVPKV-----NAALAKIKADGR 233 Query: 572 LGKIAQKWFGYTP 584 +I +KWFG P Sbjct: 234 YAEIYRKWFGAEP 246 >gi|300313535|ref|YP_003777627.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 260 Score = 84.3 bits (207), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 64/223 (28%), Positives = 100/223 (44%), Gaps = 5/223 (2%) Query: 361 GPLRTGVDATFAPHAMVKLGGGLQGFNIDLGEELARRLGRKIEIEGTEFSGLVPGLNAKK 420 G LR V A F P V QG +ID+ +A++LG K+E+ + +P L KK Sbjct: 34 GTLRVAVPADFPPFGSVDADLKPQGLDIDVATLIAKKLGVKVELIPVSSANRIPYLTTKK 93 Query: 421 YDFVLAPVTATPERAKAMLFTEGYLDTDYTFVIKKGAAPINALDDLKGKTISVNKGSAYE 480 D V++ + ER K + F+ Y + V + + DL GKTI+V +GS + Sbjct: 94 VDLVISSMGKNAEREKVIDFSAAYAPF-FNGVFGPADLSVKSAADLAGKTIAVTRGSVED 152 Query: 481 LWARDNAPKYGFKYDVYGNNADAVQAVQSGRADANLAGSTVAAWAAKQNP--AVQTTYTV 538 L P Y +N + + SG+ G+ VAA +NP +T + + Sbjct: 153 LELTKIVPASA-TVKRYEDNNSTISSFLSGQVQLVATGNVVAAAIIARNPPKKPETKFLI 211 Query: 539 KTGLVWALAFRLDDKVGRDAAGMALKCMKQDGTLGKIAQKWFG 581 K + + +K +D L K+DG+L I+QKW G Sbjct: 212 KDSPCF-IGLNKGEKKLQDKVDAILAESKKDGSLSAISQKWLG 253 >gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 84.3 bits (207), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 70/245 (28%), Positives = 119/245 (48%), Gaps = 10/245 (4%) Query: 344 AVLSASALLSAGAMAQG----GPLRTGVDATFAPHAMVK-LGGGLQGFNIDLGEELARRL 398 A+L++S +SA + Q G L+ ++ + P G L GF +D+ + LA +L Sbjct: 16 ALLASSLSVSAADLLQSVKSSGTLKVALEGNYPPFNFKDPKTGELTGFEVDVAKLLAAKL 75 Query: 399 GRKIEIEGTEFSGLVPGLNAKKYDFVLAPVTATPERAKAMLFTEGYLDTDYTFVIKKGAA 458 G K TE+SG++ GL A KYD ++ V T ER KA F++ Y + +++K Sbjct: 76 GVKPVFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSSAQLIVRKDEK 135 Query: 459 -PINALDDLKGKTISVNKGSAYELWARDNAPKYGFKYDVYGNNADAVQAVQSGRADANLA 517 +L+DLKGK + + +G+ +E A+ P G Y + + + + +GR DA L Sbjct: 136 REFKSLEDLKGKKLGLGQGTNFEQKAK-AVP--GIDVRTYPGSPEYLADLAAGRIDAALN 192 Query: 518 GSTVAAWAAKQ-NPAVQTTYTVKTGLVWALAFRLDDKVGRDAAGMALKCMKQDGTLGKIA 576 S + + K N ++ + + FR + + A AL +K DG+ + Sbjct: 193 DSLLVGYILKSTNLPLKAGSPIGAVDKIGIPFRKGNPEFKAALNKALADIKADGSFKAAS 252 Query: 577 QKWFG 581 +KWFG Sbjct: 253 EKWFG 257 >gi|300313169|ref|YP_003777261.1| amino acid transport periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 77.4 bits (189), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 62/225 (27%), Positives = 107/225 (47%), Gaps = 6/225 (2%) Query: 365 TGVDATFAPHAMVKLGGGLQGFNIDLGEELARRLGRKIEIEGTEFSGLVPGLNAKKYDFV 424 +D P M QG +++ LA+ LG ++EI +P L K D V Sbjct: 41 VAIDLGAPPFGMTNAKLEPQGSDVETARLLAKDLGVELEIVQVTGPNRIPFLMTGKADMV 100 Query: 425 LAPVTATPERAKAMLFTEGYLDTDYTFVIKKGAAPINALDDLKGKTISVNKGSAYELWAR 484 ++ + TPER K + FT+ Y + + KG I +L+DL GK ++V +G+ + Sbjct: 101 ISSFSITPERQKVVAFTQPYGASQFVVAAPKGEN-IKSLNDLVGKRVAVVRGNMQDSLLT 159 Query: 485 DNAPKYGFKYDVYGNNADAVQAVQSGRADANLAGSTVAAWAAKQNPA--VQTTYTVKTGL 542 APK G + ++A A+ SG+ DA +++A+ AKQNP+ ++T + +K + Sbjct: 160 PIAPK-GTNIVRFDDDATVTAAIISGQVDALCTPNSLASAIAKQNPSKNLETKFAIK-DI 217 Query: 543 VWALAFRLDDKVGRDAAGMALKCMKQDGTLGKIAQKWFGYTPAAD 587 +A+ R + + ++G L I Q+W G +P D Sbjct: 218 PYAIGLRKGEPALEKWLNEWISANLKNGKLPGIYQQWVG-SPMPD 261 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,874,672 Number of extensions: 78616 Number of successful extensions: 303 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 287 Number of HSP's successfully gapped: 14 Length of query: 615 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 524 Effective length of database: 1,188,985 Effective search space: 623028140 Effective search space used: 623028140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)