BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0003.p (220 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein... 327 4e-91 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 93 2e-20 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 78 7e-16 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 71 7e-14 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 66 3e-12 >gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 327 bits (839), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 187/263 (71%), Positives = 202/263 (76%), Gaps = 44/263 (16%) Query: 1 MDLGISGRRALVCASSKGLGLACAISLAREGVAVTMTARGAEVLEAAAQQVRA---ATGA 57 MDL ++G+ ALVCASSKGLG ACA SLAREGV VTM ARG E LEAAAQ++RA ATGA Sbjct: 1 MDLRLNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEVAATGA 60 Query: 58 SVTTVAGDITTPEGRALALAACPAPDILVTNAGGPKPGDFREWSREDWIAAIDANMLTPI 117 +TTV+ DITTPEGRALALAACPAPDILVTNAGGPKPGDFR+WSREDWIAA+DANMLTPI Sbjct: 61 IITTVSCDITTPEGRALALAACPAPDILVTNAGGPKPGDFRDWSREDWIAAVDANMLTPI 120 Query: 118 ELIKATVDGMMQRGFGRIVNITSGAVKAPIDILGLSNGARSGLTGFVAGLARTTVAHNVT 177 ELIKATVDGM+ R FGRIVNITS AV+APIDILGLSNGARSGLTGFVAGLAR TVAHNVT Sbjct: 121 ELIKATVDGMIARRFGRIVNITSSAVRAPIDILGLSNGARSGLTGFVAGLARKTVAHNVT 180 Query: 178 INNLLPGPF----------------------------------------QFGFSSXPWLC 197 INNLLPGPF +FG + +LC Sbjct: 181 INNLLPGPFETDRLRATASGAAQESGRSVDEILAERRKLNPAGRFGDPAEFG-DACAYLC 239 Query: 198 SAQAGFVTGQNLLMDGGAYPGTF 220 SAQAGF+TGQNLLMDGGAYPGTF Sbjct: 240 SAQAGFITGQNLLMDGGAYPGTF 262 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 92.8 bits (229), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 71/254 (27%), Positives = 110/254 (43%), Gaps = 48/254 (18%) Query: 5 ISGRRALVCASSKGLGLACAISLAREGVAVTM-----TARGAEVLEAAAQQVRAATGASV 59 +S R A V G+G A L ++G V + R + L +Q G + Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQ-----GFDI 55 Query: 60 TTVAGDITTPEGRALALAACPAP----DILVTNAGGPKPGDFREWSREDWIAAIDANMLT 115 G+++ E A A D+LV NAG + G FR+ S++DW A ID N+ + Sbjct: 56 HASEGNVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNS 115 Query: 116 PIELIKATVDGMMQRGFGRIVNITSGAVKAPIDILGLSN--GARSGLTGFVAGLARTTVA 173 + K ++GM +RGFGRI+NI+S V G +N A++G+ GF LA+ Sbjct: 116 LFNVTKQVIEGMCERGFGRIINISS--VNGQKGQFGQTNYSTAKAGIHGFTMALAQEVAT 173 Query: 174 HNVTINNLLPG-----------------------------PFQFGFSSXPWLCSAQAGFV 204 VT+N + PG P + S W+ S++ G+ Sbjct: 174 KGVTVNTVSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIA-SIVAWIASSEGGYA 232 Query: 205 TGQNLLMDGGAYPG 218 TG + ++GG Y G Sbjct: 233 TGSDFSLNGGLYMG 246 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 77.8 bits (190), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 75/245 (30%), Positives = 105/245 (42%), Gaps = 39/245 (15%) Query: 5 ISGRRALVCASSKGLGLACAISLAREGVAVTMTARGAEVLEAAAQQVRAATGASVTTVAG 64 + G+ AL+ +S G+G A A+ AR G A+ +TAR + LE A + A G + VAG Sbjct: 4 LQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALI-CAQGGRASVVAG 62 Query: 65 DI----TTPEGRALALAACPAPDILVTNAGGP---KPGDFREWSREDWIAAIDANMLTPI 117 D+ T G A+AL DI + NAG KP E S + W + AN+ + Sbjct: 63 DVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKP--LAEISPQQWQDTLHANLTSAF 120 Query: 118 ELIKATVDGMMQRGFGRIVNITSGAVKAPIDILGLS--NGARSGLTGFVAGLARTTVAHN 175 + + M+ RG G IV TS V + + G++ A++GL G V G+A Sbjct: 121 LGARCQIPAMLARGAGAIV-FTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQG 179 Query: 176 VTINNLLPGPFQFGFSSXP--------------------------WLCSAQAGFVTGQNL 209 + N LLPG P +L S A FVTG L Sbjct: 180 IRANALLPGGVDTDMGGDPQQKQWAAGLHAMKRIAQPEEIAAAALFLASPMASFVTGTAL 239 Query: 210 LMDGG 214 DGG Sbjct: 240 YADGG 244 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 70.9 bits (172), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 56/191 (29%), Positives = 91/191 (47%), Gaps = 9/191 (4%) Query: 3 LGISGRRALVCASSKGLGLACAISLAREGVAVTMTARGAEVLEAAAQQVRAATGASVTTV 62 + G+ AL+ SS G+GLA A +A+ G A+ + R A L A A Q+R A G ++ Sbjct: 15 FSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLR-AEGHTIHES 73 Query: 63 AGDITTPEGRALALAACPAP----DILVTNAGGPKPGDFREWSREDWIAAIDANMLTPIE 118 + D T+ E A+ + ILV NAG + E+ DW I N+ + Sbjct: 74 SFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFF 133 Query: 119 LIKATVDGMMQRGFGRIVNITSGAVKAPIDILGLS--NGARSGLTGFVAGLARTTVAHNV 176 +A M+ RG G+I+NI S V++ + G++ + + F G+A + + Sbjct: 134 AAQAVARHMIPRGAGKIINICS--VQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGI 191 Query: 177 TINNLLPGPFQ 187 +N L PG F+ Sbjct: 192 QVNGLGPGYFK 202 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 65.9 bits (159), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 74/251 (29%), Positives = 106/251 (42%), Gaps = 42/251 (16%) Query: 5 ISGRRALVCASSKGLGLACAISLAREGVAVTMTARGAEVLEAAAQQVRAATGASVTTVAG 64 + G+ ALV +S+G+G A A LAR+G V TA +E AA + AA G A Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVIGTAT-SESGAAAITEYLAALGPEAGRGAV 64 Query: 65 DITTPEGRALALAACPAPD-----ILVTNAGGPKPGDFREWSREDWIAAIDANMLTPIEL 119 ++AL D ILV NAG + E+W + I N+ L Sbjct: 65 LNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRL 124 Query: 120 IKATVDGMMQRGFGRIVNITS--GAVKAPIDILGLSN--GARSGLTGFVAGLARTTVAHN 175 +A + GMM+ GRI+NITS G P G N A++G+ G LAR + N Sbjct: 125 SRAVLRGMMKARTGRIINITSVVGEAGNP----GQMNYAAAKAGVAGMSRALAREIGSRN 180 Query: 176 VTINNLLPGPFQFGFS----------------------------SXPWLCSAQAGFVTGQ 207 +T+N + PG + + +L S QA ++TG Sbjct: 181 ITVNCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGT 240 Query: 208 NLLMDGGAYPG 218 L ++GG Y G Sbjct: 241 TLNVNGGMYMG 251 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 692,836 Number of extensions: 27109 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 17 Length of query: 220 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 138 Effective length of database: 1,231,600 Effective search space: 169960800 Effective search space used: 169960800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)