BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0098.p (605 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313709|ref|YP_003777801.1| L-glutamine:D-fructose-6-phosph... 1224 0.0 gi|300313838|ref|YP_003777930.1| glucosamine 6-phosphate synthas... 106 6e-24 gi|300312583|ref|YP_003776675.1| glutamine phosphoribosylpyropho... 68 3e-12 >gi|300313709|ref|YP_003777801.1| L-glutamine:D-fructose-6-phosphate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 605 Score = 1224 bits (3166), Expect = 0.0, Method: Compositional matrix adjust. Identities = 596/605 (98%), Positives = 601/605 (99%) Query: 1 MCGIVGAVAQQNVTPILLEGLKRLEYRGYDSCGVALHVDGKLQRSRSTSRVAELQQQVAQ 60 MCGIVGAVAQQNVTPILLEGLKRLEYRGYDSCGVALHVDGKLQRSRSTSRVAELQQQ+AQ Sbjct: 1 MCGIVGAVAQQNVTPILLEGLKRLEYRGYDSCGVALHVDGKLQRSRSTSRVAELQQQIAQ 60 Query: 61 SGLSGFTGIAHTRWATHGAPATHNAHPHFSRDRIALVHNGIIENHDELRAELQAAGYVFE 120 SGLSGFTGIAHTRWATHGAPATHNAHPHFSRDRIALVHNGIIENHDELRAELQA GYVFE Sbjct: 61 SGLSGFTGIAHTRWATHGAPATHNAHPHFSRDRIALVHNGIIENHDELRAELQAVGYVFE 120 Query: 121 SQTDTEVIAHLVDHMYDGDLFHTVQQAVRRLTGAYAIAVFCADEPHRVVAARQGSPLIVG 180 SQTDTEVIAHLVDHMYDGDLF+TVQQAVRRLTGAYAIAVFCADEPHRVVAARQGSPLIVG Sbjct: 121 SQTDTEVIAHLVDHMYDGDLFNTVQQAVRRLTGAYAIAVFCADEPHRVVAARQGSPLIVG 180 Query: 181 VGEGQNFVASDAMALAGTTDQIIYLEEGDVVDLQLQKVWIVDAAGRPAEREVRTVQAYTS 240 VGEGQNFVASDAMALAGTTDQIIYLEEGDVVDLQLQKVWIVDAAGRPAEREVRTVQAYTS Sbjct: 181 VGEGQNFVASDAMALAGTTDQIIYLEEGDVVDLQLQKVWIVDAAGRPAEREVRTVQAYTS 240 Query: 241 AVELGPYQHYMQKEIFEQPRAIADTLEGVVGIMPELFGDKAYKVFKEVDSILILACGTSY 300 AVELGPYQHYMQKEIFEQPRAIADTLEGVVGIMPELFGDKAYKVFKE+DSILILACGTSY Sbjct: 241 AVELGPYQHYMQKEIFEQPRAIADTLEGVVGIMPELFGDKAYKVFKEIDSILILACGTSY 300 Query: 301 YAGLTAKYWLESIAKIPVNVEIASEYRYRDSVPNPKSLVVTISQSGETADTLAALKHARG 360 YAGLTAKYWLESIAKIPVNVEIASEYRYRDSVPNPKSLVVTISQSGETADTLAALKHAR Sbjct: 301 YAGLTAKYWLESIAKIPVNVEIASEYRYRDSVPNPKSLVVTISQSGETADTLAALKHARS 360 Query: 361 LGMHHTLTICNVATSAMVRECELAYITRAGVEVGVASTKAFTTQLAALFLLTLALAQTRG 420 LGM HTLTICNVATSAMVRECELAYITRAGVEVGVASTKAFTTQLAALFLLTLALAQTRG Sbjct: 361 LGMQHTLTICNVATSAMVRECELAYITRAGVEVGVASTKAFTTQLAALFLLTLALAQTRG 420 Query: 421 LLTEEQEAEHLKAMRHLPAALQSVLALEPSIVAWAEAFARRENALFLGRGLHYPIALEGA 480 LLTEEQEAEHLKAMRHLPAALQSVLALEPSIVAWAE+FARRENALFLGRGLHYPIALEGA Sbjct: 421 LLTEEQEAEHLKAMRHLPAALQSVLALEPSIVAWAESFARRENALFLGRGLHYPIALEGA 480 Query: 481 LKLKEITYIHAEAYAAGELKHGPLALVTDQMPVVTIAPNDALVEKLKSNMQEVRARGGQL 540 LKLKEITYIHAEAYAAGELKHGPLALVTDQMPVVTIAPNDALVEKLKSNMQEVRARGGQL Sbjct: 481 LKLKEITYIHAEAYAAGELKHGPLALVTDQMPVVTIAPNDALVEKLKSNMQEVRARGGQL 540 Query: 541 YVFADGDSRITSAEGLHVIRMPEHYGVLSPILHVVPLQLLSYHTALARGTDVDKPRNLAK 600 YVFADGDS I+SAEGLHVIRMPEHYGVLSPILHVVPLQLLSYHTALARGTDVDKPRNLAK Sbjct: 541 YVFADGDSHISSAEGLHVIRMPEHYGVLSPILHVVPLQLLSYHTALARGTDVDKPRNLAK 600 Query: 601 SVTVE 605 SVTVE Sbjct: 601 SVTVE 605 >gi|300313838|ref|YP_003777930.1| glucosamine 6-phosphate synthase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 106 bits (264), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 96/326 (29%), Positives = 165/326 (50%), Gaps = 46/326 (14%) Query: 290 SILILACGTSYYAGLTAKYWLES-----IAKIPVNVEIAS--EYRYRDSVPNPKSLVVTI 342 +I+ +A G+S +A Y + + +A +P+++ + + RD+ L + + Sbjct: 37 NIVTVARGSSDHAATYCAYLIMARLGRIVASLPMSLVTLNHAPLQVRDA------LAIAV 90 Query: 343 SQSGETADTLAALK--HARGLGMHHTLTICNVATSAMVRECELAYITRAGVEVGVASTKA 400 SQSG++ D + ++ HA+G T+ + N A S + + + AGVE VA+TK+ Sbjct: 91 SQSGQSPDVVEPIRYFHAQG---ATTVALVNQADSPLASAAQWSLPLHAGVESSVAATKS 147 Query: 401 FTTQLAALFLLTLALAQTRGLLTEEQEAEHLKAMRHLPAALQSVLALEPSIVAWAEA--- 457 F L A LLT + +A L A+ LP AL++ A + W++A Sbjct: 148 FIASLVASALLTAHW---------QDDAALLNALAQLPDALRAACACD-----WSDAVQA 193 Query: 458 FARRENALFLGRGLHYPIALEGALKLKEITYIHAEAYAAGELKHGPLALVTDQMPVVTIA 517 + + +GRG+ +P+ALE ALK KE I AEA++ E+KHGP+AL+ + P++ A Sbjct: 194 LTPADRIMVVGRGIGFPLALEAALKCKETCAIQAEAFSGAEIKHGPIALIEEGYPLLMFA 253 Query: 518 ---PNDALVEKLKSNMQEVRARGGQLYVFADGDSRITSAEGLHVIRMPEHYGVLSPILHV 574 P+ A + L + E+R RG ++ + A D + L V P+ L PI+ + Sbjct: 254 IRGPSQAGMLALAA---EMRVRGAKVLLAAPEDV-VERDLTLPVAATPD----LDPIVAI 305 Query: 575 VPLQLLSYHTALARGTDVDKPRNLAK 600 +++ + ARG D D+PR+L K Sbjct: 306 QAFYVMAAQLSAARGMDADRPRHLNK 331 >gi|300312583|ref|YP_003776675.1| glutamine phosphoribosylpyrophosphate amidotransferase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 67.8 bits (164), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 59/222 (26%), Positives = 102/222 (45%), Gaps = 31/222 (13%) Query: 1 MCGIVGAVAQQNVTPILLEGLKRLEYRGYDSCGVALHVDGKLQRSRSTSRVAELQQQVAQ 60 MCGIVG V+ V +L + L L++RG D+ G+A + ++ V ++ + Sbjct: 1 MCGIVGIVSHSPVNQMLYDALLLLQHRGQDAAGIATNHSNGFSMHKANGLVRDVFRTRNM 60 Query: 61 SGLSGFTGIAHTRWATHGAPAT-HNAHPHFSRDR--IALVHNGIIENHDELRAEL-QAAG 116 L G TGI R+ T G+ ++ A P + I L HNG + N ++L+ E+ + Sbjct: 61 RSLPGNTGIGQVRYPTAGSSSSEEEAQPFYVNAPFGIILAHNGNLTNAEQLKIEMFKNDR 120 Query: 117 YVFESQTDTEVIAHLVDHM-------YDGD---LFHTVQQAVRRLTGAYAIAVFCAD--- 163 + +DTEV+ +++ H Y D LF V +R+ G+YA+ A Sbjct: 121 RHINTDSDTEVLLNVLAHEIQQATTGYSLDPAALFKAVAMVHKRVRGSYAVVAQIAGYGL 180 Query: 164 ----EPHRVVAARQGSPLIVGVGEGQN----FVASDAMALAG 197 +P+ + P+ +G E +AS+++AL G Sbjct: 181 LGFRDPYGI------RPMCLGFNESDKGVEYMMASESVALEG 216 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,847,963 Number of extensions: 78692 Number of successful extensions: 263 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 260 Number of HSP's successfully gapped: 3 Length of query: 605 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 514 Effective length of database: 1,188,985 Effective search space: 611138290 Effective search space used: 611138290 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)