BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0094.p (358 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313691|ref|YP_003777783.1| CDP-glucose-4,6-dehydratase [He... 617 e-178 gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 66 5e-12 gi|300313504|ref|YP_003777596.1| galactose-1-phosphate uridylylt... 61 1e-10 gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [H... 59 6e-10 >gi|300313691|ref|YP_003777783.1| CDP-glucose-4,6-dehydratase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 617 bits (1590), Expect = e-178, Method: Compositional matrix adjust. Identities = 297/358 (82%), Positives = 325/358 (90%), Gaps = 1/358 (0%) Query: 1 MANFWRDKRVFITGHTGFKGSWLTLWLHLMGAKVSGYALPAPTNPSLFHLARLQNCVQTT 60 MANFWRDKRVFITGHTGFKGSWLTLWLHLMGAKVSGY+LPAPT+PSLFHLARL NCVQTT Sbjct: 1 MANFWRDKRVFITGHTGFKGSWLTLWLHLMGAKVSGYSLPAPTSPSLFHLARLHNCVQTT 60 Query: 61 IGDVRDPQHLAETLAAVQPDVVFHLAAQPLVRDSYATPVDTYATNVMGTVHLLEAVRHTP 120 GDVRDP+HLAET+AAVQPDVVFHLA QP + SY TP+DT+ATNVMGT HLLEAVR TP Sbjct: 61 TGDVRDPKHLAETMAAVQPDVVFHLAGQPQIEHSYLTPLDTFATNVMGTAHLLEAVRSTP 120 Query: 121 SVRSVVVVTSDKCYENREWLWGYREDDPMGGHDPYSSSKGAAELVTSAYRRSFFASNPDN 180 SVR+VVVV+S+KCYENREWLWGYREDDPMGGHDP+SSSKGAAELVT+AYRRSFF+++PD+ Sbjct: 121 SVRAVVVVSSNKCYENREWLWGYREDDPMGGHDPFSSSKGAAELVTAAYRRSFFSADPDS 180 Query: 181 TVGIASVRAGNVIGGGDFAADRLIPDFVRALEKEEPVVIRNPHAVRPWQFVLEPLSGYLQ 240 VG+ASVR GNVIGGGDFAADRLIPDF+RALE ++PV IR PHAVRPWQFVLEPLSGYLQ Sbjct: 181 PVGVASVRTGNVIGGGDFAADRLIPDFIRALETDQPVTIRQPHAVRPWQFVLEPLSGYLQ 240 Query: 241 IAQLLYEQGGAFSEGWNFGPADTDLQTVSQLCATFNHSLQKNDAGQVEVRLDTQANGPHE 300 I ++LYE G +++E WNFGP DTDLQTVSQLCATFNHSLQKND VEVRL+ QA Sbjct: 241 IGKMLYEHGASYAEAWNFGPTDTDLQTVSQLCATFNHSLQKNDVEPVEVRLE-QAGATLS 299 Query: 301 AALLRLDISKVRQRLDWIPKMELYTALELTAQWYAGYLNNEDLRTLSENQIAFYQSLN 358 A+LRLD SK RQRLDWIP+MELYTALELTAQWYAGYLNNEDLR LSENQIAFYQSL Sbjct: 300 EAVLRLDTSKARQRLDWIPRMELYTALELTAQWYAGYLNNEDLRALSENQIAFYQSLT 357 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 65.9 bits (159), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 54/189 (28%), Positives = 91/189 (48%), Gaps = 14/189 (7%) Query: 10 VFITGHTGFKGSWLTLWLHLMGAKVSGYALPAPTNPSLFHLARLQNCVQTTI----GDVR 65 + +TG G+ GS + L G +V + ++P + R+Q T+ GD+R Sbjct: 3 ILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPE--SVKRVQQITGKTLSLVKGDIR 60 Query: 66 DPQHLAETLAAVQPDVVFHLAAQPLVRDSYATPVDTYATNVMGTVHLLEAVRHTPSVRSV 125 + + L L + + V H A V +S P+ Y NV+GTV LLEA++ T V+++ Sbjct: 61 NREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQAT-GVKTI 119 Query: 126 VVVTSDKCYENREWLWGYREDDPMGGHDPYSSSKGAAELVTSAYRRSFFASNPDNTVGIA 185 V +S Y + ++L E P+ +PY SK +V R ++ ++PD IA Sbjct: 120 VFSSSATVYGDPQYL-PLDEAHPLSASNPYGRSK----IVIEDMLRDYYRAHPDWK--IA 172 Query: 186 SVRAGNVIG 194 +R N +G Sbjct: 173 LLRYFNPVG 181 >gi|300313504|ref|YP_003777596.1| galactose-1-phosphate uridylyltransferase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 66/224 (29%), Positives = 100/224 (44%), Gaps = 28/224 (12%) Query: 4 FWRDKRVFITGHTG-FKGSWLTLWLHLMGAKVSGYALPAPTNPSLFHLARLQNCVQTTIG 62 + D + ITG TG F + L +L+ ++ ++ L +A ++ IG Sbjct: 1 MFDDSVLMITGGTGSFGQTVLNRFLNTGVREIRVFSRDEKKQEDL-RIALNNEKLRFYIG 59 Query: 63 DVRDPQHLAETLAAVQPDVVFHLAAQPLVRDSYATPVDTYATNVMGTVHLLEAVRHTPSV 122 DVRDP L + V D VFH AA V P++ TNV+GT ++L V V Sbjct: 60 DVRDPDSLRAAMKGV--DYVFHAAALKQVPSCEFYPMEAVKTNVIGTENVLR-VSSECGV 116 Query: 123 RSVVVVTSDKCYENREWLWGYREDDPMGGHDPYSSSKGAAELVTSAYRRSFFASNPDNTV 182 R VVV+++DK P+ + SK AE V A R+ TV Sbjct: 117 RRVVVLSTDKAVY------------PI---NAMGISKAMAEKVMVAAART---QRQGETV 158 Query: 183 GIASVRAGNVIGGGDFAADRLIPDFVRALEKEEPVVIRNPHAVR 226 A+ R GNV+ + +IP FV ++ E+P+ I +P+ R Sbjct: 159 FCAT-RYGNVMA----SRGSVIPLFVSQIKAEKPITITDPNMTR 197 >gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [Herbaspirillum seropedicae SmR1] Length = 338 Score = 58.9 bits (141), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 78/349 (22%), Positives = 137/349 (39%), Gaps = 47/349 (13%) Query: 10 VFITGHTGFKGSWLTLWLHLMGAKVSGYALPAPTNPSLFHLARLQ-----NCVQTTIGDV 64 +F+TG GF G+ L L AP + ++ ++ +L + + + GD Sbjct: 2 LFVTGGAGFIGANYILHF-----------LAAPHDEAVLNIDKLTYAGNPDNLASVAGDA 50 Query: 65 R---------DPQHLAETLAAVQPDVVFHLAAQPLVRDSYATPVDTYATNVMGTVHLLEA 115 R D +A A +P V H AA+ V S A P TNV GT +L A Sbjct: 51 RYLFSQTDICDTAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSA 110 Query: 116 V----RHTPSVRSVVV----VTSDKCYENREW-LWGYREDDPMGGHDPYSSSKGAAELVT 166 P+ +++D+ + + + E P + PYS+SK A++ + Sbjct: 111 AYDYWSTLPAAEQAAFRFLHISTDEVFGSLAADAPPFTETSPYAPNSPYSASKAASDHLV 170 Query: 167 SAYRRSFFASNPDNTVGIASVRAGNVIGGGDFAADRLIPDFVRALEKEEPVVIR-NPHAV 225 A+ ++ + + N G F ++LIP + +P+ I + V Sbjct: 171 RAFHSTY-------GLPVLITHCSNNYGPFHF-PEKLIPLVITNALAGQPLPIYGDGQQV 222 Query: 226 RPWQFVLEPLSGYLQIAQLLYEQGGAFSEGWNFGPADTDLQTVSQLCATFNHSLQKNDAG 285 R W V + +++ G +++ G N T+L+ V LC + L G Sbjct: 223 RDWLHVADHCRA-IELVLARARPGESYNIGGN--SEKTNLEVVLTLCDLLD-ELAPKAQG 278 Query: 286 QVEVRLDTQANGPHEAALLRLDISKVRQRLDWIPKMELYTALELTAQWY 334 Q ++ + P +D SK+++ L W P+ L T QW+ Sbjct: 279 QYRDQICFVQDRPGHDRRYAVDTSKIQRELGWSPQHAFRQGLRQTVQWF 327 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,161,900 Number of extensions: 46717 Number of successful extensions: 142 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 4 Length of query: 358 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 272 Effective length of database: 1,212,660 Effective search space: 329843520 Effective search space used: 329843520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)