BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0088.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 368 e-103 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 310 1e-85 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 303 2e-83 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 268 5e-73 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 262 5e-71 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 368 bits (944), Expect = e-103, Method: Compositional matrix adjust. Identities = 190/425 (44%), Positives = 282/425 (66%), Gaps = 13/425 (3%) Query: 14 SAAEAAP-APMSAARTRRLIVASSIGNALEWFDFVAYGFFAVTISKLFFPTGDETVSLLV 72 S+ AAP A ++ + RR I++SSIGNALEWFD + YG FAV I+K FFPTGD++VSLL+ Sbjct: 2 SSNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLL 61 Query: 73 TFATFGLSYLFRPLGAILLGWYGDKVGRKPALLVSLSMTVAGTLICTVIPTYETIGVAAP 132 TFATFG+S+ RPLGA++LG Y D+ GRK AL++S+ + GT + +P+Y +IG+ AP Sbjct: 62 TFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAP 121 Query: 133 IGILVARILLALSAGGEFGSATALLTETGSKRRAFMASWQFSSQGAAALMAALAGFLLNQ 192 GI + +++ SAGGEFGS+TA L E RR F +SWQ +SQG + L+AAL G +LN Sbjct: 122 AGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGISLLLAALFGAVLNN 181 Query: 193 WLTPEQISAWGWRLPFAFGLLIAPVGLYIRRHIDEVPMAKKA--QVAGEDDASWGLQVTR 250 LTPEQ+++WGWR+PF FGLLIAP G+YIRRH+DE P K++ + ++ Q R Sbjct: 182 MLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKMR 241 Query: 251 VLVAMGILSVSTAATYLMLLYMPTYAIKQLHLPQSTALYRTRLKKTPPPAAVTGAILMIG 310 +L+ G + ++T + YL LY+PTYA+KQL LP ++ +V G I+ IG Sbjct: 242 LLIGAGSVIMATVSVYLS-LYIPTYAVKQLGLPAWSSF---------AAMSVAGLIMFIG 291 Query: 311 APFMGMLADRVGRARIMVVSTVLTLATIYGSFVWVIAMPTVWVMMAIMIWAGILKAVYFG 370 +P +G L+D++GR M+ S+ L + Y FV++ P ++ + G+L +YF Sbjct: 292 SPLVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFA 351 Query: 371 SLGVVLSSIFPPATRVRGMGISYSLGATLFGGFTPFMVTWLVGKTGNPVSPSYYLMVCAA 430 ++ +L+ IFP ATR GM ++Y++ T+FGGF ++TWL+ TG+ +S SY+++ A Sbjct: 352 AMPALLADIFPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAV 411 Query: 431 ISLLC 435 +SL+ Sbjct: 412 LSLIA 416 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 310 bits (795), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 177/431 (41%), Positives = 255/431 (59%), Gaps = 20/431 (4%) Query: 23 MSAARTRRLIVASSIGNALEWFDFVAYGFFAVTISKLFFPTGDETVSLLVTFATFGLSYL 82 +S + RR IVA+ IGN LEWFDF Y FFAV I+KLFFPTG++ SLL+ ATFG+ + Sbjct: 14 VSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFF 73 Query: 83 FRPLGAILLGWYGDKVGRKPALLVSLSMTVAGTLICTVIPTYETIGVAAPIGILVARILL 142 RP+G ILLG Y D+VGRK AL V++ + GT + + PTYE+IG+ AP+ I+VAR+L Sbjct: 74 MRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQ 133 Query: 143 ALSAGGEFGSATALLTETGSKR-RAFMASWQFSSQGAAALMAALAGFLLNQWLTPEQISA 201 SAGGE GSATA LTE +R RA+ +SW +S G A L+ A G + L +++ Sbjct: 134 GFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALAS 193 Query: 202 WGWRLPFAFGLLIAPVGLYIRRHIDEVPMAKKAQVAGEDDASWGLQVTRVL-----VAMG 256 WGWRLPF G++I PVG YIR H++E P + + + +V R + Sbjct: 194 WGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLK---EVIRNFPRETSASFS 250 Query: 257 ILSVSTAATYLMLLYMPTYAIKQLHLPQSTALYRTRLKKTPPPAAVTGAILMIGAPFMGM 316 ++ + T TY++L YMPTY++K L LPQ+T V G +M+ AP +G Sbjct: 251 MVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFI---------AGMVGGLCIMVLAPIVGR 301 Query: 317 LADRVGRARIMVVSTVLTLATIYGSFVWVIAMPTVWVMMAIMIWAGILKAVYFGSLGVVL 376 LADR+GR ++ S L L Y F ++ + P++ ++ + G L A Y G + Sbjct: 302 LADRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAF 361 Query: 377 SSIFPPATRVRGMGISYSLGATLFGGFTPFMVTWLVGKTGNPVSPSYYLMVCAAISLLCV 436 S +FP G+ ++Y+L T+FGGF F +TWL+ TG+ ++P++Y+M+ AAIS V Sbjct: 362 SELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISF--V 419 Query: 437 SIYHFRLKKYL 447 R +YL Sbjct: 420 GTRFVREPQYL 430 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 303 bits (776), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 169/415 (40%), Positives = 246/415 (59%), Gaps = 19/415 (4%) Query: 27 RTRRLIVASSIGNALEWFDFVAYGFFAVTISKLFFPTGDETVSLLVTFATFGLSYLFRPL 86 +R ++A+ +GNALEW+DF+ YGFF+ I++LFFP +E SLLV ATFG+ + RP+ Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 87 GAILLGWYGDKVGRKPALLVSLSMTVAGTLICTVIPTYETIGVAAPIGILVARILLALSA 146 G +LLG Y D+ GRK A+ V + + I PTY TIG+AAPI I+VAR+L + Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 147 GGEFGSATALLTETG-SKRRAFMASWQFSSQGAAALMAALAGFLLNQWLTPEQISAWGWR 205 GGE+ S+TA L E+ + +R SWQ Q A A G L+ + L+PE + +WGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 206 LPFAFGLLIAPVGLYIRRHIDEVPMAKKAQVAGEDDAS--WGLQVTR-----VLVAMGIL 258 LPF GLLI PVGL+IRRH+ E + A + +A+ L V + VLV+MG + Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETD--DFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQV 243 Query: 259 SVSTAATYLMLLYMPTYAIKQLHLPQSTALYRTRLKKTPPPAAVTGAILMIGAPFMGMLA 318 T A Y++L+ MPT+A KQL LP A + A+L PF G L+ Sbjct: 244 INGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQM---------IAVALLTAVIPFAGALS 294 Query: 319 DRVGRARIMVVSTVLTLATIYGSFVWVIAMPTVWVMMAIMIWAGILKAVYFGSLGVVLSS 378 DR+GR +++ S++ L +Y FVWV A P++ ++ + + I+ FG + LS Sbjct: 295 DRLGRRTVLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSE 354 Query: 379 IFPPATRVRGMGISYSLGATLFGGFTPFMVTWLVGKTGNPVSPSYYLMVCAAISL 433 FP R G+ ++Y+L A LFGGF PF+VTWL +G+PV+P++Y++ A I + Sbjct: 355 QFPTRVRSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGV 409 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 268 bits (686), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 161/401 (40%), Positives = 227/401 (56%), Gaps = 13/401 (3%) Query: 38 GNALEWFDFVAYGFFAVTISKLFFPTGDETVSLLVTFATFGLSYLFRPLGAILLGWYGDK 97 GN LE FDF YGF+A ISK FFP+ +E VSL++TF TFG +L RPLGAI+LG Y D+ Sbjct: 22 GNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAGFLMRPLGAIILGSYIDR 81 Query: 98 VGRKPALLVSLSMTVAGTLICTVIPTYETIGVAAPIGILVARILLALSAGGEFGSATALL 157 VGR+ L+V+L++ GTL+ +P Y TIGVAAPI +L R+L SAG E G + L Sbjct: 82 VGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQGFSAGVELGGVSVYL 141 Query: 158 TETGS-KRRAFMASWQFSSQGAAALMAALAGFLLNQWLTPEQISAWGWRLPFAFGLLIAP 216 E + + F SWQ +SQ AA + +AL G+L++Q L+P QIS WGWR+PF G +I P Sbjct: 142 AEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDWGWRIPFFIGCMIVP 201 Query: 217 VGLYIRRHI---DEVPMAKKAQVAGEDDASWGLQVTRVLVAMGILSVSTAATYLMLLYMP 273 IRR + DE K+ GE +S V+ M ++ ++T + YL+ +Y P Sbjct: 202 AIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVAGMMMVVMTTVSFYLITVYTP 261 Query: 274 TYAIKQLHLPQSTALYRTRLKKTPPPAAVTGAILMIGAPFMGMLADRVGRARIMVVSTVL 333 T+ L L S +L T G P MG L+DRVGR ++V T+L Sbjct: 262 TFGKNVLKLSASDSLIVT---------FCVGLSNFCWLPIMGALSDRVGRRPVLVAFTLL 312 Query: 334 TLATIYGSFVWVIAMPTVWVMMAIMIWAGILKAVYFGSLGVVLSSIFPPATRVRGMGISY 393 TL T Y W+++ + ++ +W L A Y G+ V L+ + P R G ++Y Sbjct: 313 TLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHVRTVGFSLAY 372 Query: 394 SLGATLFGGFTPFMVTWLVGKTGNPVSPSYYLMVCAAISLL 434 SL +FGGFTP + TWL+ +G+ +P Y++ A LL Sbjct: 373 SLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLL 413 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 262 bits (669), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 157/415 (37%), Positives = 232/415 (55%), Gaps = 22/415 (5%) Query: 38 GNALEWFDFVAYGFFAVTISKLFFPTGDETVSLLVTFATFGLSYLFRPLGAILLGWYGDK 97 GN +E FDF +GF+A ISK FFP+ +E SL++TF TFG +L RPLGAI+LG Y D+ Sbjct: 20 GNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMRPLGAIILGAYVDR 79 Query: 98 VGRKPALLVSLSMTVAGTLICTVIPTYETIGVAAPIGILVARILLALSAGGEFGSATALL 157 VGR+ L+V+L++ GT++ +P+Y TIG AP+ +L+ R+L SAG E G + L Sbjct: 80 VGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFSAGVELGGVSVYL 139 Query: 158 TETGS-KRRAFMASWQFSSQGAAALMAALAGFLLNQWLTPEQISAWGWRLPFAFGLLIAP 216 E + + F SWQ +SQ A ++AA G+LL+ LTP ++S WGWR+PF G LI P Sbjct: 140 AEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGWRIPFFIGCLIVP 199 Query: 217 VGLYIRRHIDEVP--MAKK-----AQVAGEDDASWGLQVTRVLVAMGILSVSTAATYLML 269 V IRR + E M +K Q+ +W L V+ M ++S++T + YL+ Sbjct: 200 VLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKL----VIAGMMLVSMTTVSFYLIT 255 Query: 270 LYMPTYAIKQLHLPQSTALYRTRLKKTPPPAAVTGAILMIGAPFMGMLADRVGRARIMVV 329 +Y PT+ L L +L T G I P MG L+DRVGR ++V Sbjct: 256 VYTPTFGKNVLKLSTEASLIVT---------LCVGLSNFIWLPIMGALSDRVGRRPLLVT 306 Query: 330 STVLTLATIYGSFVWVIAMPTVWVMMAIMIWAGILKAVYFGSLGVVLSSIFPPATRVRGM 389 TVLT+ T Y + W++ + M+ + +W L A Y G++ V L+ + P R G Sbjct: 307 FTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVRTAGF 366 Query: 390 GISYSLGATLFGGFTPFMVTWLVGKTGNPVSPSYYLMVCAAISLLCVSIYHFRLK 444 ++YSL +FGGFTP + T L+ T + +P + M AA+ L ++ +R K Sbjct: 367 SLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIW-MSTAAVCGLIATLILYRNK 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,340,546 Number of extensions: 57078 Number of successful extensions: 382 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 334 Number of HSP's successfully gapped: 13 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)