BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0082.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogena... 487 e-139 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 100 1e-22 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 80 2e-16 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 73 2e-14 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 69 5e-13 >gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 487 bits (1254), Expect = e-139, Method: Compositional matrix adjust. Identities = 242/255 (94%), Positives = 249/255 (97%), Gaps = 1/255 (0%) Query: 10 MTSPSS-GIALVTGAARRIGRHIALALARAGWDVAVHYNRSREEADTLVREIEALGRRAL 68 MTSPSS GIALVTGAARRIGRHIALALA++GWDVAVHYNRSREEADTLVREIE LGRRAL Sbjct: 1 MTSPSSAGIALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIETLGRRAL 60 Query: 69 AVQGELGDEAQVRSLLPQAAPLGRITCVVNNASLFDYDDAASFGNDRLLRHMSANVGAPV 128 AVQ ELGDE QVRSLLPQAAPLGRI+CVVNNASLFDYDDAASFGN+RLLRHMSANVGAPV Sbjct: 61 AVQAELGDETQVRSLLPQAAPLGRISCVVNNASLFDYDDAASFGNERLLRHMSANVGAPV 120 Query: 129 LLAQALFEATPEGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRV 188 LLAQAL+EATP+GQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRV Sbjct: 121 LLAQALYEATPQGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRV 180 Query: 189 VGVAPGLTMVSGDQTEDQFDKAHTVTPLGRGSTPEDIAAAVCYVASARAVTGTTLLVDGG 248 VGVAPGLTMVSGDQTE+QF KAH VTPLGRGSTPEDIAAAVCYVASARAVTGTTLLVDGG Sbjct: 181 VGVAPGLTMVSGDQTEEQFGKAHQVTPLGRGSTPEDIAAAVCYVASARAVTGTTLLVDGG 240 Query: 249 QHLLPLSRDVMFVAK 263 QHLLPLSRDVMFVAK Sbjct: 241 QHLLPLSRDVMFVAK 255 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 100 bits (249), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 82/247 (33%), Positives = 128/247 (51%), Gaps = 16/247 (6%) Query: 17 IALVTGAARRIGRHIALALARAGWDVAVHYNRSREEADTLVREIEALGRRALAVQGELGD 76 + LVTG +R IG AL + G+ VAV+Y +R AD +V +I+A G RA+AV G++ Sbjct: 7 VILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAVGGDVAR 66 Query: 77 EAQVRSLLPQA-APLGRITCVVNNASLFDYDDA-ASFGNDRLLRHMSANVGAPVLLAQAL 134 E V L LGR+T +VNNA + D+ +R+ R + ANV +L A+ Sbjct: 67 EEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLCAREA 126 Query: 135 FEATPE---GQQAVVINL--LDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPK-VRV 188 + GQ ++NL + KL P +++ Y SK A+ + T LA+ +A + +RV Sbjct: 127 VKRMSTRHGGQGGAIVNLSSMAAKLGGPG-EYVDYAASKGAIDAFTIGLAKEVAAEGIRV 185 Query: 189 VGVAPGLTMV----SGDQTEDQFDKAHTVTPLGRGSTPEDIAAAVCYVASARA--VTGTT 242 V PGL SG + + D+ P+ RG + ++A A+ ++ S A TGT Sbjct: 186 NAVRPGLIYTDIHASGGEA-GRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYATGTF 244 Query: 243 LLVDGGQ 249 + V GG+ Sbjct: 245 IDVSGGR 251 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 78/241 (32%), Positives = 114/241 (47%), Gaps = 10/241 (4%) Query: 15 SGIALVTGAARRIGRHIALALARAGWDVAVHYNRSREEADTLVREIEALGRRALAVQGEL 74 S +A V G +R IG I LA G VA Y S ++A LV ++A G RALA+Q + Sbjct: 17 SKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQADS 76 Query: 75 GDEAQVRSLLPQAAP-LGRITCVVNNASLFDYDDAASFGNDRLLRHMSANVGAPVLLAQA 133 D A ++ + AA GR+ +VNNA + F + L R ++ NV + + Q Sbjct: 77 ADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRSVFVATQE 136 Query: 134 LFEATPEGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPK-VRVVGVA 192 EG + + I + P Y +SK+AL T LA+ L P+ + V V Sbjct: 137 AARYMGEGGRVISIGSTNADRI-PFAGGAVYAMSKSALVGLTKGLARDLGPRGITVNNVQ 195 Query: 193 PGLTMVSGDQTEDQFDKAHTVT---PLGRGSTPEDIAAAVCYVASARA--VTGTTLLVDG 247 PG V D D A ++T L R E+IA+ V Y+A + +TG L++DG Sbjct: 196 PG--PVDTDMNPASGDFAASLTGLMALPRYGKAEEIASFVAYLAGPESGYITGANLMIDG 253 Query: 248 G 248 G Sbjct: 254 G 254 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 73.2 bits (178), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 76/244 (31%), Positives = 116/244 (47%), Gaps = 12/244 (4%) Query: 17 IALVTGAARRIGRHIALALARAGWDVAVHYNRSREEADTLVREIEALGRRA--LAVQGEL 74 +ALVTGA+R IGR IA LAR G V + S A + + ALG A AV Sbjct: 10 VALVTGASRGIGRAIATELARQGAKV-IGTATSESGAAAITEYLAALGPEAGRGAVLNVN 68 Query: 75 GDEAQVRSLLPQAAPLGRITCVVNNASLFDYDDAASFGNDRLLRHMSANVGAPVLLAQAL 134 EA V + G ++ +VNNA + A ++ ++ N+ A L++A+ Sbjct: 69 DAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAV 128 Query: 135 FEATPEGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPK-VRVVGVAP 193 + + +IN+ NP ++Y +KA + + LA+ + + + V VAP Sbjct: 129 LRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAP 188 Query: 194 GLTMVSGDQT----EDQFDKAHTVTPLGRGSTPEDIAAAVCYVASARA--VTGTTLLVDG 247 G + D T E Q PLGR E+IA AV ++AS +A +TGTTL V+G Sbjct: 189 GF--IDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNG 246 Query: 248 GQHL 251 G ++ Sbjct: 247 GMYM 250 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 68.6 bits (166), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 68/245 (27%), Positives = 118/245 (48%), Gaps = 10/245 (4%) Query: 17 IALVTGAARRIGRHIALALARAGWDVAVHYNRSREEADTLVREIEALGRRALAVQGELGD 76 + ++GA+ IG A A A G + VHYN S +EA+ + E+ A G +++ + ++ D Sbjct: 8 VVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSITIGADVRD 67 Query: 77 EAQVRSLLPQA-APLGRITCVVNNASLFDYDDAASFGNDRLLRH-MSANVGAPVLLAQAL 134 + + + + +A A +GRI ++NNA D L ++ N + V +A+ Sbjct: 68 TSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVAFTRAV 127 Query: 135 FEATPEGQQAVVINLLD-QKLYNPNPDFLSYTLSKAALHSATTLLAQA-LAPKVRVVGVA 192 +IN+ + P L Y SK + + T +A+ LA K+RV VA Sbjct: 128 IPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRVNAVA 187 Query: 193 PGLTMVSGDQ---TEDQFDKAHTVTPLGRGSTPEDIAAAVCYVASARA---VTGTTLLVD 246 PG+ M + T +Q + P+GR P++ + A Y+AS + VTG + V+ Sbjct: 188 PGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTGQIIDVN 247 Query: 247 GGQHL 251 GGQ++ Sbjct: 248 GGQYM 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 822,864 Number of extensions: 33976 Number of successful extensions: 194 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 18 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)