BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0075.p (163 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 279 9e-77 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 88 3e-19 gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase tra... 88 4e-19 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 87 6e-19 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 87 6e-19 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 279 bits (713), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 139/163 (85%), Positives = 145/163 (88%) Query: 1 MGFAEVDLRALLGSVVGDLSSVADHGGIDLGLSDQGDDGRPVRVQGNADALRILFNNLVD 60 M F +VDLRALLGSVVGDLSSVA+HGGIDLGLSDQGDDGRP+RV GNADALRILFNNLVD Sbjct: 282 MSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNADALRILFNNLVD 341 Query: 61 NALRYTPAGGVVDVGFEFPAGGVCAVVIQDSGPGIAESELPRVFDRFYRAQRAQEQGSSR 120 NALRYTPAGGVVDVG + G C VV+QDSGPGIAESELPRVFDRFYRAQ +QE SR Sbjct: 342 NALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRFYRAQHSQEGSGSR 401 Query: 121 GAFGSGLGLAIVRQIADLHRVPVELRNTGRGLQARVVFTLLPA 163 AFGSGLGLAIVRQIADLHRVPVELRNTG GLQARVVF L PA Sbjct: 402 AAFGSGLGLAIVRQIADLHRVPVELRNTGHGLQARVVFPLSPA 444 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 88.2 bits (217), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 68/171 (39%), Positives = 89/171 (52%), Gaps = 18/171 (10%) Query: 2 GFAEVDLRALLGSVVGDLSSVADHGGIDLGLSDQGDDGRPVRVQGNADALRILFNNLVDN 61 G V+L L SVV D ++ IDLG +Q D P+ + GN LR L +NL+DN Sbjct: 219 GMIPVELNELARSVVQDWVPMSFTQRIDLGF-EQPD--HPILITGNPVMLRELLSNLLDN 275 Query: 62 ALRYTPA--GGVVDVGFEFPAGGVCAVV-IQDSGPGIAESELPRVFDRFYRAQRAQEQGS 118 AL YTP G V V GG A++ ++D+GPGI +E VF+RFYR GS Sbjct: 276 ALHYTPHDGNGRVTVRARTDEGGGLAILEVEDNGPGIPVAERANVFERFYRILGTPSTGS 335 Query: 119 SRGAFGSGLGLAIVRQIADLHRVPVELRNTGR-------GLQARVVFTLLP 162 G GLGLAIVR+IA H +E+ + R G RV F ++P Sbjct: 336 -----GLGLGLAIVREIAQQHEAALEITSNPRSTDPKYPGCVFRVSFRMMP 381 >gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 459 Score = 87.8 bits (216), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 60/132 (45%), Positives = 74/132 (56%), Gaps = 11/132 (8%) Query: 4 AEVDLRALLGSVVGDLSSVADHGGIDLGLSDQGDDGRPVRVQGNADALRILFNNLVDNAL 63 A VDL A+ V+ +LS +A GIDLGL + V GN L + NL+DNAL Sbjct: 309 APVDLLAVSAMVLEELSGLALKKGIDLGLETTLESAM---VNGNEAQLMGMLMNLIDNAL 365 Query: 64 RYTPAGGVVDVGFEFPAGGVCAVVIQDSGPGIAESELPRVFDRFYRAQRAQEQGSSRGAF 123 RYTP GG V VG E + + + D+GPGI RVF+RFYR A +Q Sbjct: 366 RYTPEGGKVTVGLEKKRSQMI-ITVSDNGPGIPAEVRERVFERFYR-NAAPDQP------ 417 Query: 124 GSGLGLAIVRQI 135 GSGLGLAIVR+I Sbjct: 418 GSGLGLAIVREI 429 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 87.4 bits (215), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 60/147 (40%), Positives = 80/147 (54%), Gaps = 14/147 (9%) Query: 18 DLSSVADHGGIDLGLSDQGDDGRPVRVQGNADALRILFNNLVDNALRYTPAGGVVDVGFE 77 +LS A I L ++GD V ++ NA L L NL+DNA+RYTPAGG V + Sbjct: 339 ELSQQAVSREIGLAFEEEGD----VSIEANALLLHELMVNLLDNAIRYTPAGGHVTLRVR 394 Query: 78 FPAG----GVCAVVIQDSGPGIAESELPRVFDRFYRAQRAQEQGSSRGAFGSGLGLAIVR 133 + V + ++DSGPGI +E +VF+ FYRA Q G+GLGL+IVR Sbjct: 395 AQSSPGQPTVALLEVEDSGPGIPAAEREKVFEPFYRAGATQHVNPG----GTGLGLSIVR 450 Query: 134 QIADLHRVPVEL--RNTGRGLQARVVF 158 +IA +HR V L G GL R+VF Sbjct: 451 EIAAMHRASVTLDAPAQGSGLVVRIVF 477 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 87.4 bits (215), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 62/162 (38%), Positives = 81/162 (50%), Gaps = 16/162 (9%) Query: 3 FAEVDLRALLGSVVGDLSSVADHGGIDLGLSDQGDDGRPVRVQGNADALRILFNNLVDNA 62 E+ L L+ V AD IDLG D RP R++G+ L L +NL+DNA Sbjct: 309 LEELSLDKLVEESVQHFIEEADKKRIDLGF-----DLRPARLRGDRFLLSDLIDNLIDNA 363 Query: 63 LRYTPAGGVVDVGFEFPAGGVCAVVIQDSGPGIAESELPRVFDRFYRAQRAQEQGSSRGA 122 +RY+P G V V + ++DSGPGIA +FDRFYRA + Sbjct: 364 VRYSPPHGTVTVRC-LEEQDATVLSVEDSGPGIAPEHRELIFDRFYRA--------NDKV 414 Query: 123 FGSGLGLAIVRQIADLH--RVPVELRNTGRGLQARVVFTLLP 162 GSGLGLAIVR+IA H + V+ + G+G V F L P Sbjct: 415 AGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPLAP 456 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.142 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 588,330 Number of extensions: 27346 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 20 Length of query: 163 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 85 Effective length of database: 1,250,540 Effective search space: 106295900 Effective search space used: 106295900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)