BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0074.p (437 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine... 827 0.0 gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine... 396 e-111 gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine... 259 3e-70 gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirill... 96 6e-21 gi|300312039|ref|YP_003776131.1| trypsin family periplasmic seri... 60 5e-10 >gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine endoprotease [Herbaspirillum seropedicae SmR1] Length = 494 Score = 827 bits (2135), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/432 (95%), Positives = 426/432 (98%) Query: 6 TDFSGIVQRYGPAVVNISVTGKARKVADDDDNGLDPNDPFSEFFKRFGPQLQMPQQPRIM 65 TDFSGIVQRYGPAVVNISVTGKARKVADDDDNGLDPNDPFSEFFKRFGPQLQMPQQPRIM Sbjct: 63 TDFSGIVQRYGPAVVNISVTGKARKVADDDDNGLDPNDPFSEFFKRFGPQLQMPQQPRIM 122 Query: 66 HGLGSGFIISPDGLILTNAHVVDGAQEVVVKLTDRREFKAKVLGIDKQSDIAVIRIDAKN 125 HGLGSGFIISPDGLILTNAHVVDGAQEVVVKLTDRREFKAKVLGIDKQSDIAVIRIDAKN Sbjct: 123 HGLGSGFIISPDGLILTNAHVVDGAQEVVVKLTDRREFKAKVLGIDKQSDIAVIRIDAKN 182 Query: 126 LPTVQIGDPSRVKVGQPVLAIGSPYGFDNTATAGIISAKSRSLPDDNYVPFIQTDVAVNP 185 LPTVQIGDPSRVKVGQPVLAIGSPYGFDNTATAGIISAKSRSLPDDNYVPFIQTDVAVNP Sbjct: 183 LPTVQIGDPSRVKVGQPVLAIGSPYGFDNTATAGIISAKSRSLPDDNYVPFIQTDVAVNP 242 Query: 186 GNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSIPIDVAMKVEQQLVTHGKVTRGRLGV 245 GNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSIPIDVAMKVEQQLVTHGKVTRGRLGV Sbjct: 243 GNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSIPIDVAMKVEQQLVTHGKVTRGRLGV 302 Query: 246 SVQDLNQALSESFGLKKAEGALVSSVEKGSPADKAGLQAGDVILSFDGHAIDHSVDLPTL 305 SVQDLNQALS+SFGLKKAEGALVSSVEKGSPADKAGLQAGDVI+SF+GHAIDHSVDLPTL Sbjct: 303 SVQDLNQALSDSFGLKKAEGALVSSVEKGSPADKAGLQAGDVIISFNGHAIDHSVDLPTL 362 Query: 306 VADTAPGSSKPIQVMRGGKVLTMNVTVGEMKQAKNEAKPGKSDDQGRLGLAVRPLDKDEQ 365 VADT PGS+KP++VMR GKV T+NVTVGEMKQAKN+AK KSDDQGRLGLAVRPLDKDEQ Sbjct: 363 VADTPPGSTKPMEVMRAGKVRTINVTVGEMKQAKNDAKASKSDDQGRLGLAVRPLDKDEQ 422 Query: 366 AQIGGQKGLLVEDASGPAAVAGIQSGDVILALNGTPVSSVAQLRKLASEAGKNVALLVER 425 AQ+GGQKGLLVED SGPAAVAGIQSGDVILA+NGTPVSSVAQLRKLA++AGKNVALLVER Sbjct: 423 AQLGGQKGLLVEDVSGPAAVAGIQSGDVILAVNGTPVSSVAQLRKLATDAGKNVALLVER 482 Query: 426 GDEKIFVPLALN 437 GDEKIFVPLALN Sbjct: 483 GDEKIFVPLALN 494 >gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 492 Score = 396 bits (1018), Expect = e-111, Method: Compositional matrix adjust. Identities = 227/459 (49%), Positives = 299/459 (65%), Gaps = 27/459 (5%) Query: 2 VAAATDFSGIVQRYGPAVVNISVTGKARKVADDDDNGLDPNDPFSEFFKRFG----PQLQ 57 VAA DF+ IV+R GP VVNI T + R+ A DP EFF+RF P+ Q Sbjct: 35 VAALPDFADIVERTGPGVVNIRTTERVRQNAQGQGGVDDPE--MQEFFRRFFGIPIPRQQ 92 Query: 58 MPQQPR-------------IMHGLGSGFIISPDGLILTNAHVVDGAQEVVVKLTDRREFK 104 P PR + G+GSGFIIS DG I+TNAHVV+GA EV V LTD+REFK Sbjct: 93 QPGTPRGNGKQGGQGQQEEVPRGVGSGFIISADGFIMTNAHVVEGASEVYVTLTDKREFK 152 Query: 105 AKVLGIDKQSDIAVIRIDAKNLPTVQIGDPSRVKVGQPVLAIGSPYGFDNTATAGIISAK 164 AK++G D ++D+AV++ID NLP + +GD +++VG+ VLAIGSP+G +NT TAGI+SAK Sbjct: 153 AKIVGSDTRTDVAVLKIDGSNLPRLNMGDSDKIRVGEWVLAIGSPFGLENTVTAGIVSAK 212 Query: 165 SRSLPDDNYVPFIQTDVAVNPGNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSIPIDV 224 +R D Y+P IQTDVAVNPGNSGGPL +L G+VIGINSQIYS++GGF G+SF++PID Sbjct: 213 ARDTGD--YLPLIQTDVAVNPGNSGGPLINLKGEVIGINSQIYSRSGGFMGISFAVPIDE 270 Query: 225 AMKVEQQLVTHGKVTRGRLGVSVQDLNQALSESFGLKKAEGALVSSVEKGSPADKAGLQA 284 A++V QL G+VTRGR+GV + ++ + ++ES GL +A+GALV VE G PA+KAGL+A Sbjct: 271 ALRVADQLKASGRVTRGRIGVQIGEVTKDVAESLGLARAQGALVQRVEAGGPAEKAGLEA 330 Query: 285 GDVILSFDGHAIDHSVDLPTLVADTAPGSSKPIQVMRGGKVLTMNVTVGEM---KQAKNE 341 GD+IL ++G AI+ DLP +V T PG+ + + R G ++VTV E+ K + E Sbjct: 331 GDIILKYNGAAIERPSDLPRMVGSTKPGAKATVSIWRKGSARDVSVTVVELEADKPKQAE 390 Query: 342 AKPGKSDDQ-GRLGLAVRPLDKDEQAQIGGQKGLLVEDASGPAAVAGIQSGDVILALNGT 400 K K D+ LGL V L ++ ++ G+LVE SG AA AGI+ GDVI LN Sbjct: 391 EKKAKPDNAPNSLGLVVSDLSDAQRKELKVDNGVLVEAVSGAAAAAGIRPGDVIQRLNEV 450 Query: 401 PVSSVAQLRKLAS--EAGKNVALLVERGDEKIFVPLALN 437 V Q +L S +A K A+LV RGD FVPL N Sbjct: 451 DVKDAKQFAQLVSKLDAKKRAAVLVRRGDSSQFVPLRPN 489 >gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 393 Score = 259 bits (662), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 144/319 (45%), Positives = 201/319 (63%), Gaps = 8/319 (2%) Query: 17 PAVVNISVTGKARKVADDDDNGLDPNDPFSEFFKRFGPQLQMPQQPRIMHGLGSGFIISP 76 P+VVNI T +AR N DPF F K FG Q QQ LGSG I+SP Sbjct: 69 PSVVNIFTTTEARPQKSPFQN-----DPF--FRKFFGDQFDEQQQDDKQSSLGSGVIVSP 121 Query: 77 DGLILTNAHVVDGAQEVVVKLTDRREFKAKVLGIDKQSDIAVIRIDAKNLPTVQIGDPSR 136 G ILTN HVV+ A ++ V L D R+ AKV+GID ++D+AVI+ID NLP + +G P Sbjct: 122 QGYILTNNHVVEAADKIEVALADGRKASAKVVGIDPETDLAVIKIDLPNLPAITLGHPEN 181 Query: 137 VKVGQPVLAIGSPYGFDNTATAGIISAKSRS-LPDDNYVPFIQTDVAVNPGNSGGPLFDL 195 VG VLAIG+P+G T T GI+SA R+ L + + FIQTD A+NPGNSGG L D Sbjct: 182 SSVGDVVLAIGNPFGVGQTVTMGIVSALGRNHLGINTFENFIQTDAAINPGNSGGALVDT 241 Query: 196 NGQVIGINSQIYSQTGGFQGLSFSIPIDVAMKVEQQLVTHGKVTRGRLGVSVQDLNQALS 255 NG ++GIN+ IYS+TGG G+ F+IP+ A V + +++HG+V RG +GV QD+ L+ Sbjct: 242 NGNLLGINTAIYSRTGGNLGIGFAIPMSTAKTVMEAIISHGQVVRGWIGVEPQDITPELA 301 Query: 256 ESFGLKKAEGALVSSVEKGSPADKAGLQAGDVILSFDGHAIDHSVDLPTLVADTAPGSSK 315 ESFGL K GA+++ V KG PAD+AG++ GD+++S D + + ++ ++A PG Sbjct: 302 ESFGLGKKTGAIIAGVLKGGPADRAGMRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQI 361 Query: 316 PIQVMRGGKVLTMNVTVGE 334 + V+R + +N+TVG+ Sbjct: 362 AMTVLRKSQETKLNITVGK 380 >gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 95.9 bits (237), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 65/164 (39%), Positives = 98/164 (59%), Gaps = 14/164 (8%) Query: 69 GSGFIISPDGLILTNAHVVDGAQEVVVKLTDRREFKAKVLGIDKQSDIAVIRID---AKN 125 G+GF I G +L+N HVV A+ V V+L RE A+VL +D D+A+++ + + Sbjct: 271 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 330 Query: 126 LPTVQIGDPSRVKVGQPVLAIGSPYG--FDNTATAGIISAKSRSLPDDNYVPFIQTDVAV 183 LP +++ +P +G+ V A+GSP G F+ T T GI+S R L N F+Q+DVA+ Sbjct: 331 LP-LRLDEPG---LGEDVYALGSPLGDTFNTTLTRGILSG-VRLL---NGQEFLQSDVAI 382 Query: 184 NPGNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSIPIDVAMK 227 PG+SGGPL D +GQV+GI G G++F IP+ A++ Sbjct: 383 LPGSSGGPLLDRSGQVVGITVAGLGAR-GMAGMNFFIPLASAVE 425 >gi|300312039|ref|YP_003776131.1| trypsin family periplasmic serine protease [Herbaspirillum seropedicae SmR1] Length = 256 Score = 59.7 bits (143), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 58/206 (28%), Positives = 96/206 (46%), Gaps = 44/206 (21%) Query: 68 LGSGFIISPDGL-ILTNAHVV------------DGAQEVVVKLTDRREFKAKVLGIDKQS 114 +G+GF++ DGL ++TNAHVV D + +V + + +A +L +D+ Sbjct: 54 VGTGFVVG-DGLTVVTNAHVVAAASGEERKDSKDNLRVLVARGANVEAREAALLVMDRLH 112 Query: 115 DIAVIRIDAKNLPTVQIGDPSRVKVGQPVLAIGSP----YGFDNTATAGIISA------- 163 D+A++R+ P +++GD + GQ + G P GF +ISA Sbjct: 113 DLALLRLSGAPEPALRVGDSDSAREGQMLAFTGFPLTAILGFHPVTHRAMISAITVVATP 172 Query: 164 --KSRSLPDDNYV-------PFIQTDVAVNPGNSGGPLFD-LNGQVIGINSQIYSQT--- 210 +RSL V P Q D PG SG PL+D +G V+G+ +++ + T Sbjct: 173 APNARSLDAKAIVQLQRSAYPVFQLDGTAYPGFSGSPLYDPESGVVLGVINKVLNATKES 232 Query: 211 --GGFQGLSFSIPIDVAMKVEQQLVT 234 G++++IP K QQL+T Sbjct: 233 AISAPSGITYAIP----AKFVQQLMT 254 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,398,287 Number of extensions: 61247 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 164 Number of HSP's successfully gapped: 5 Length of query: 437 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 349 Effective length of database: 1,203,190 Effective search space: 419913310 Effective search space used: 419913310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)