BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0073.p (612 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium... 1062 0.0 gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium... 236 5e-63 gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans... 182 5e-47 gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 107 2e-24 >gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium-efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 606 Score = 1062 bits (2746), Expect = 0.0, Method: Compositional matrix adjust. Identities = 569/605 (94%), Positives = 590/605 (97%) Query: 1 MDEQLLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF 60 MDEQLLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF Sbjct: 1 MDEQLLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF 60 Query: 61 GVVLLLFLIGLELDPKRLWALRRSIFGWGSVQVAAVSLALFAAALAAGVPWRIGLIGALG 120 GVVLLLFLIGLELDPKRLWALRRSIFGWGS+QVAAVSLAL AAALAA VPWRIGLIGALG Sbjct: 61 GVVLLLFLIGLELDPKRLWALRRSIFGWGSIQVAAVSLALLAAALAASVPWRIGLIGALG 120 Query: 121 MSLSSTAIALATLGERKLTATPAGAAGFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGW 180 MSLSSTAIALATLGERKLTATPAG+A FSILLFQDIAAIPMMAAVPLLGVAASQGSGQGW Sbjct: 121 MSLSSTAIALATLGERKLTATPAGSAAFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGW 180 Query: 181 LHAGQVALVIALLIFGGRVLIRPILRIIAKTDMREIFTAFALLLVISTCLLMQSVGMSMA 240 LHAGQVALVIALLIFGGRVL+RPILRIIAKTDMREIFTAFALLLVI+TCLLMQSVGMSMA Sbjct: 181 LHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSVGMSMA 240 Query: 241 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQPWRILGMVAG 300 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVF AQPWRILGMV G Sbjct: 241 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRILGMVLG 300 Query: 301 FLTIKTGVMWFLGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPEISSMLVV 360 FL IKT VMW LGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPE SS+LVV Sbjct: 301 FLAIKTCVMWALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPETSSLLVV 360 Query: 361 VVALSMLITPLLLLVHDRLLVRWLEGGKRRPEDKIGPQDNQVIIAGFGRFGQIVGRLLAA 420 VVALSML+TPLLLLVHDRLLVRW+EGGKRRPEDKI PQD+ VIIAGFGRFGQI+GRLLAA Sbjct: 361 VVALSMLVTPLLLLVHDRLLVRWMEGGKRRPEDKIDPQDHAVIIAGFGRFGQIIGRLLAA 420 Query: 421 NDIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKAKVLVIAIDDAQAALDM 480 N IGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKA+VLVIAIDDAQAAL+M Sbjct: 421 NGIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEM 480 Query: 481 VEKVREEFPHLQIAARARNVTHFYDLMDRGVTVLERETFESALHMGRQVLQQLDFGAYRA 540 V+KVREEFPHLQIAARARNVTH+YDLMDRGVTVLERETFESALHMGRQVLQQLDFGAYRA Sbjct: 481 VDKVREEFPHLQIAARARNVTHYYDLMDRGVTVLERETFESALHMGRQVLQQLDFGAYRA 540 Query: 541 REAAMKFRSHNLKTLAALYPHYKDQAQMVSMAVQAREELQAMFAGDREAMQRDHTHDQWR 600 REAAMKFR+HNLKTL ALYPHYK+QAQMVSMAVQAREEL+AMFAGDR+AM+R+H+HDQWR Sbjct: 541 REAAMKFRAHNLKTLEALYPHYKNQAQMVSMAVQAREELEAMFAGDRQAMEREHSHDQWR 600 Query: 601 EKPAP 605 +P P Sbjct: 601 GEPPP 605 >gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 663 Score = 236 bits (601), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 190/592 (32%), Positives = 291/592 (49%), Gaps = 28/592 (4%) Query: 5 LLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVL 64 L + L L AAV V + + L +LGYL+ GI+IGP GLG + + EFGVV Sbjct: 5 LELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLGFAEDNETTHALGEFGVVF 64 Query: 65 LLFLIGLELDPKRLWALRRSIFGWGSVQVAAVSLALFAAALAA--------GVPWRIGLI 116 L+F IGLE +L A+R +FG G QVA A A + W+ Sbjct: 65 LMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQAAFA 124 Query: 117 GALGMSLSSTAIALATLGERKLTATPAGAAGFSILLFQDIAAIPMMAAVPLLGVAASQGS 176 +++SSTAI L ER T G +LLFQD+A +P++ VP A + S Sbjct: 125 LGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVP----ALANES 180 Query: 177 GQ-----GWLHAGQVALVIALLIFGGRVLIRPILRIIAKTDMREIFTAFALLLVISTCLL 231 G GW AG+ +V+ALL+F G L+R RI+ K +E+F LL+ + + Sbjct: 181 GNIMVTLGW-AAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWI 239 Query: 232 MQSVGMSMALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQP 291 + G+S+ALG F+AG+L++++E++H +E D++ F+ +LLGLFFI +GM ++ + Sbjct: 240 TEKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHW 299 Query: 292 WRILGMVAGFLTIKTGVMWFLGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLT 351 + +L ++ G + +K G++ L R FG G L L+Q GEF F++ A K++ Sbjct: 300 FLVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVD 359 Query: 352 PEISSMLVVVVALSMLITPLLLLVHDRLLVR-----WLEGG---KRRPEDKIGPQDNQVI 403 P + +++ + LSML TP +L D ++++ W+ + +G Q VI Sbjct: 360 PMLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQ-KHVI 418 Query: 404 IAGFGRFGQIVGRLLAANDIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADK 463 IAGFGR GQ + RLL I L+ DP+ + R G V YGDA R + L AG + Sbjct: 419 IAGFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHR 478 Query: 464 AKVLVIAIDDAQAALDMVEKVREEFPHLQIAARARNVTHFYDLMDRGVTVLERETFESAL 523 A LV+ +AL ++ E P L + R+ + L+ G T + E E +L Sbjct: 479 AAALVVTYASTPSALKVLHHAAELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEGSL 538 Query: 524 HMGRQVLQQLDFGAYRAREAAMKFRSHNLKTLAALYPHYKD-QAQMVSMAVQ 574 + L L R R +L + D +A V M V+ Sbjct: 539 MLASHALVMLGVPLRRVVHRVQDARDERYASLRGYFHGMSDGEADGVGMNVR 590 >gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 182 bits (463), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 160/522 (30%), Positives = 254/522 (48%), Gaps = 21/522 (4%) Query: 21 IAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVLLLFLIGLELDPKRLWA 80 +A RL + ++GYL+AGI IGP+ G + + E +E GV+LL+F +GL K L + Sbjct: 24 LAHRLKMPPLVGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVGLHFSLKDLLS 83 Query: 81 LRRSIFGWGSVQVAAVSLALFAAALAAGVPWRIGLIGALGMSLSSTAIALATLGERKLTA 140 ++ Q+ +L A G P G + L +S++ST + L L ER+L Sbjct: 84 VKNIAIPGAVAQITIATLLGMALGWLLGWPVGEGFLFGLALSVASTVVLLKALQERRLVE 143 Query: 141 TPAGAAGFSILLFQDIAAIPMMAAVPLL-GVAASQG---SGQGWLHA-----GQVALVIA 191 T G L+ +DIA + + +P L G+ +G SG L G+V A Sbjct: 144 TQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGNAVLLTLGITLGKVVAFAA 203 Query: 192 LLIFGGRVLIRPILRIIAKTDMREIFTAFALLLVISTCLLMQSV-GMSMALGTFLAGVLL 250 +++ GR +I IL IA T RE+F L + + L V G+S ALG F AG++L Sbjct: 204 VMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSAYVFGVSFALGAFFAGMIL 263 Query: 251 ADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQPWRILGMVAGFLTIKTGVMW 310 ++SE H + P + LFF++VGM D + +P +L V + K+ Sbjct: 264 SESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVAREPLLVLATVLIIVVGKSLGAL 323 Query: 311 FLGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPEISSMLVVVVALSMLITP 370 + R FG P G L + L+Q GEF+FI+ ++ +L+P +++ LS+L+ P Sbjct: 324 LIVRAFGYPNGTALTISASLAQIGEFSFILATLGLSLDLLSPMARDLILGGAILSILLNP 383 Query: 371 LLLLVHDRLLVRWLEGGKRRPEDKIGPQ----DNQVIIAGFGRFGQIVGRLLAANDIGAT 426 LL + DR R + P+ PQ + V++ GFGR G+ +G L A Sbjct: 384 LLFSLLDRYEARQ----PKAPQGGDEPQKVELQDHVVLVGFGRVGRGIGEQLRAQGKPFV 439 Query: 427 VLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKAKVLVIAIDDAQAALDMVEKVRE 486 V++ + ++ LR G YG+A + +LL+ A D+A+ L++AI + A ++E E Sbjct: 440 VIEAQLENLEALRAAGVPALYGNAAQSELLQAAAIDRARWLLVAIPEVFEAGQVIENALE 499 Query: 487 EFPHLQIAARARNVTHFYDLMDRG---VTVLERETFESALHM 525 P LQ+ ARA + L G V + ERE L + Sbjct: 500 LNPGLQVVARAHSDAEIEHLEKHGAQRVIMGEREIARGMLEL 541 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 107 bits (268), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 104/379 (27%), Positives = 191/379 (50%), Gaps = 11/379 (2%) Query: 7 IALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWG--LGLITEVKHILEFSEFGVVL 64 +A++ L+A + V + +L VLGY++AG++IGP LI + K + SE GV+ Sbjct: 11 MAIIMLIAGIVTV-VFNKLKQPVVLGYIVAGVIIGPHTPPYDLIQDEKTVHILSELGVIF 69 Query: 65 LLFLIGLELDPKRLWALRRSIFGWGSVQVAAVSLALFAAALAAGVPWR---IGLIGALGM 121 LLF +GLE K+L + + F + ++ + + L G W+ +GA+ + Sbjct: 70 LLFSLGLEFSLKKLAKVGATAFIGAAAEILLMIWIGYEIGLYFG--WKRMDAIFLGAM-L 126 Query: 122 SLSSTAIALATLGERKLTATPAGAAGFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGWL 181 ++SST I + L E + F IL+ +DI AI M+A + + + S S + + Sbjct: 127 AVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGVATSGSVDSTEVFT 186 Query: 182 HAGQVALVIALLIFGGRVLIRPILRIIAKTDMREIFTAFALLLVISTCLLMQSVGMSMAL 241 G++ L + + + G + + +L I++ E+ L ++ CLL+ + S+AL Sbjct: 187 TVGKLVLFMTVSLVVGILAVPRLLSFISRFKSNEMLLVAVLGVLFGFCLLVMKLQYSVAL 246 Query: 242 GTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFLAQPWRILGMVAGF 301 G FL G ++A+S + H +E +EP + + +FF+A+G+ D V L + W + ++ Sbjct: 247 GAFLVGAVMAESRHLHRIERLVEPIRDMFSAIFFVAIGLLFDPNV-LVKYWMPITVITLA 305 Query: 302 LTIKTGVMWFLGRCFGIPRGQQ-LFFAFLLSQGGEFAFIVFGAAVTAKVLTPEISSMLVV 360 + + LG G+ + L+Q GEF+FI+ + KV + + ++V Sbjct: 306 VVFGKLISCGLGTFIAGQGGRTPMRVGMGLAQIGEFSFIIAALGQSLKVTSDFLYPIVVA 365 Query: 361 VVALSMLITPLLLLVHDRL 379 V A++ L+TP L+ D L Sbjct: 366 VSAVTALLTPYLIKAADPL 384 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,594,849 Number of extensions: 65747 Number of successful extensions: 381 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 373 Number of HSP's successfully gapped: 4 Length of query: 612 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 521 Effective length of database: 1,188,985 Effective search space: 619461185 Effective search space used: 619461185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)