BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0061.p (249 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 80 1e-16 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 72 3e-14 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 68 6e-13 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 67 1e-12 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 67 2e-12 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 80.5 bits (197), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 79/254 (31%), Positives = 105/254 (41%), Gaps = 34/254 (13%) Query: 1 MSERHFLVVGGSKGSGLALVEHLLAAGQRVSVLARSASPLPSSTLLNWQQLDVTDGGALR 60 ++ +V GGS+G GLA + A G RV V R A L Q + GA+ Sbjct: 7 FEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLE-------QAVREIGNGAVG 59 Query: 61 AALDACARSQGKIDSA-VFMQRNRATGDAFDTDLAVALTATRNA----------IDHLL- 108 DA K+D R +A D A A TA+ A D L Sbjct: 60 VPGDAA-----KLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFN 114 Query: 109 ---------AQGHFSLDESGNSIVMVSSIADHYVAPEQDLGYHVSKAGLVQLARYYALQL 159 Q L G +IV+ S+A P Q L Y+ SKA + AR + L Sbjct: 115 LNVRGLLFSVQKALPLMSQGGAIVLNGSVAGVKGFPGQSL-YNASKAAVRSFARSWTADL 173 Query: 160 GPHGIRVNVVSPCVVIKEAAREYYRQNSWITDRFERFIPLRRAGSSRDIINAILFLTGEQ 219 GIRVNVV+P R+Y + D ++ +PL R G +I A+LFL + Sbjct: 174 KERGIRVNVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAE 233 Query: 220 SSYITGQNIVVDGG 233 SSYI G + VDGG Sbjct: 234 SSYIAGVELFVDGG 247 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 72.4 bits (176), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 71/243 (29%), Positives = 111/243 (45%), Gaps = 15/243 (6%) Query: 4 RHFLVVGGSKGSGLALVEHLLAAGQRVSV--LARSASPLPSSTLLNWQ------QLDVTD 55 R LV GG+ G G ++ + L A G V V + A+ + L+ Q+D+ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 56 GGALRAALDACARSQGKIDSAVFMQRNRATGDAFDTDLAVAL-TATRNAIDHLLAQGH-- 112 ++ AA + G+ D V T D L L T N LLA H Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 113 -FSLDESGNSIVMVSSIADHYVAPEQDLGYHVSKAGLVQLARYYALQLGPHGIRVNVVSP 171 + + IV +SSI+ A Y SKA ++ L R A++L +GI VN V+P Sbjct: 129 RLMVKQQWGRIVNISSISG-IRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAP 187 Query: 172 CVVIKEAAREYYRQNSWITDRFERFIPLRRAGSSRDIINAILFLTGEQSSYITGQNIVVD 231 V + + + + + + R +P+RR G++ +I +A+ +L E+S+YITG I VD Sbjct: 188 GPVDTPMTQAMHSEQT--RESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVD 245 Query: 232 GGL 234 GG Sbjct: 246 GGF 248 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 68.2 bits (165), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 42/116 (36%), Positives = 58/116 (50%), Gaps = 2/116 (1%) Query: 119 GNSIVMVSSIADHYVAPEQDLGYHVSKAGLVQLARYYALQLGPHGIRVNVVSPCVVIKEA 178 G ++V ++SIA H EQ L Y SKAGL R A +LGPHGIRVN V+P + + E Sbjct: 125 GGAMVNITSIAGHR-GFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTEL 183 Query: 179 AREYYRQNSWITDRFERFIPLRRAGSSRDIINAILFLTGEQSSYITGQNIVVDGGL 234 A + + R P R S D+ +I L ++ + G + VDGG Sbjct: 184 AEAAWSDPAKSGPMMARH-PSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGF 238 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 67.0 bits (162), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 77/253 (30%), Positives = 112/253 (44%), Gaps = 41/253 (16%) Query: 7 LVVGGSKGSGLALVEHLLAAGQRVSVLARSASPLPSSTLLNWQQL----------DVTDG 56 V GGS+G G A+V+ L A G V+ S SP + L++ Q D D Sbjct: 21 FVQGGSRGIGAAIVQRLAAEGATVAFTYVS-SPDKAQALVSSVQAKGGRALAIQADSADA 79 Query: 57 GALRAALDACARSQGKIDSAVFMQRNRATG--DAF-----DTDLAV----ALTATRNAID 105 AL+ A+ A + G++D V A G D F D LAV AT+ A Sbjct: 80 AALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRSVFVATQEAAR 139 Query: 106 HLLAQGHFSLDESGNSIVMVSSIADHYVAPEQDLGYHVSKAGLVQLARYYALQLGPHGIR 165 ++ E G I + S+ AD + Y +SK+ LV L + A LGP GI Sbjct: 140 YM--------GEGGRVISIGSTNADRIPFAGGAV-YAMSKSALVGLTKGLARDLGPRGIT 190 Query: 166 VNVVSPCVV---IKEAAREYYRQNSWITDRFERFIPLRRAGSSRDIINAILFLTGEQSSY 222 VN V P V + A+ ++ + L R G + +I + + +L G +S Y Sbjct: 191 VNNVQPGPVDTDMNPASGDF-------AASLTGLMALPRYGKAEEIASFVAYLAGPESGY 243 Query: 223 ITGQNIVVDGGLS 235 ITG N+++DGG S Sbjct: 244 ITGANLMIDGGFS 256 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 66.6 bits (161), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 75/253 (29%), Positives = 116/253 (45%), Gaps = 28/253 (11%) Query: 1 MSERHFLVVGGSKGSGLAL-VEHLLAA-GQRVSVL-ARSASPLPSSTLLNWQQL----DV 53 + ++ ++ GG+ +GL L+AA G R+ +L A P ++ L Q L DV Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVADV 62 Query: 54 TDGGALRAALDACARSQGKID----SAVFMQRNR---ATGDAFDTDLAVALTATRNAIDH 106 TD A AA A + G+ID +A Q + TG +D L V+L T Sbjct: 63 TDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMSQA 122 Query: 107 LLAQGHFSLDESGNSIVMVSSIADH-----YVAPEQDLGYHVSKAGLVQLARYYALQLGP 161 +L +SG SIV +SS++ P Y +KAG++ LAR A + GP Sbjct: 123 VLP--AMQAQQSG-SIVCISSVSAQRGGGILGGPH----YSAAKAGVLGLARAMAREFGP 175 Query: 162 HGIRVNVVSPCVVIKEAAREYYRQNSWITDRFERFIPLRRAGSSRDIINAILFLTGEQSS 221 GIRVN ++P ++ + ++ + IPL R G D+ +FL + S Sbjct: 176 QGIRVNCITPGLISTDINKDILSAEK--KAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQ 233 Query: 222 YITGQNIVVDGGL 234 Y TG + V+GG+ Sbjct: 234 YCTGVTLDVNGGM 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 782,654 Number of extensions: 31500 Number of successful extensions: 201 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 24 Length of query: 249 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 166 Effective length of database: 1,226,865 Effective search space: 203659590 Effective search space used: 203659590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)