BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0054.p (351 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 181 1e-46 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 145 4e-36 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 124 9e-30 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 84 2e-17 gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 79 5e-16 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 181 bits (458), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 129/367 (35%), Positives = 191/367 (52%), Gaps = 38/367 (10%) Query: 7 MRAAILAELQ--------QPLIIDEVELPPQLEVGQVLVKIGVSGVCGSQLGEIDGAKGE 58 ++AA+L E+ QPL I+E++L P G+VLV++ +G+C S L I G + Sbjct: 3 IQAAVLREMSDAHPFAVSQPLKIEELDLDPP-GPGEVLVRMKAAGLCHSDLSVITGVR-- 59 Query: 59 DKYLPHLLGHEASGIVEEVGPGVRFVKPGDRVVLHWRKSQG-----IESLPP-------- 105 + +P LGHEAS V EVG GV ++ GD VVL + S G +E P Sbjct: 60 PRPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAAT 119 Query: 106 -----------KYTWRGKPLNAGL-IATFNEYAIVSENRVTRIDPSSDMDVAALFGCAVT 153 + + +P++ L ++ F ++A+VS +I+ D AALFGCAV Sbjct: 120 NTVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVL 179 Query: 154 TGFGVAENNADIRLGESVVVFGAGGVGLNIIQAASLRSAYPIIAVDLHEGRLALAKQVGA 213 TG G A N A+++ G + V G GGVGL + A A +IAVDLH+ +LALA+ +GA Sbjct: 180 TGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGA 239 Query: 214 THLINASQVDARTALLELTGKQGIDVFIDNTGQPSIIEMGYELTKAQGRVVLVGVPRAGR 273 T +NA DA + LT + G+D + G +E Y +T+ G V G+P G+ Sbjct: 240 TATVNARDPDAVEKVRALT-RGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQ 298 Query: 274 NISIYSLPL-HFEKTISGSHGGEAVPHVDIPRYTRLLDAGLIQLKPLITEHFKLDDINVA 332 + ++ + L E+T+ GS+ G VP DIPRY L AG + + L+ E L DIN Sbjct: 299 HWALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRG 358 Query: 333 IQRMRSG 339 R+ G Sbjct: 359 FDRLHGG 365 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 145 bits (367), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 118/359 (32%), Positives = 178/359 (49%), Gaps = 30/359 (8%) Query: 8 RAAILAELQQPLIIDEVELPPQLEVGQVLVKIGVSGVCGSQLGEIDGAKGEDKYLPHLLG 67 RAA+ + +PL I+EVEL + G+VLV+I +G+C + + GA E + P +LG Sbjct: 4 RAAVAWQAGKPLTIEEVELGGP-KAGEVLVEIKATGICHTDYYTLSGADPEGIF-PAILG 61 Query: 68 HEASGIVEEVGPGVRFVKPGDRVVLHW---------------------RKSQGIESLP-- 104 HE +G+V +VGPGV+ +K D V+ + R +QG +P Sbjct: 62 HEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDA 121 Query: 105 -PKYTWRGKPLNAGL-IATFNEYAIVSENRVTRIDPSSDMDVAALFGCAVTTGFGVAENN 162 +++ GKPL + +TF+ Y +V E + +I + D GC VTTG G Sbjct: 122 TSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVTTGVGAVLFT 181 Query: 163 ADIRLGESVVVFGAGGVGLNIIQAASLRSAYPIIAVDLHEGRLALAKQVGATHLINASQV 222 A + G +VVVFG GG+GLN+IQAA + A II VDL+ R A+A++ G T IN V Sbjct: 182 AKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKDV 241 Query: 223 -DARTALLELTGKQGIDVFIDNTGQPSIIEMGYELT-KAQGRVVLVGVPRAGRNISIYSL 280 + A++ +T G D + G + + E K G+ +++GV AG+ IS Sbjct: 242 PNVVDAIIGMT-DGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPF 300 Query: 281 PLHFEKTISGSHGGEAVPHVDIPRYTRLLDAGLIQLKPLITEHFKLDDINVAIQRMRSG 339 L + GS G A D+P+ G + + LIT L+ IN M+SG Sbjct: 301 QLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSG 359 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 124 bits (312), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 107/356 (30%), Positives = 170/356 (47%), Gaps = 29/356 (8%) Query: 5 KTMRAAILAELQQPLIIDEVELPPQLEVGQVLVKIGVSGVCGSQLGEIDGAKGEDKYLPH 64 + M+AA++ E +PL I++V +P GQ+LVK SGVC + L G P Sbjct: 3 QMMKAAVVREFGKPLSIEQVPVPTPAP-GQILVKFEASGVCHTDLHAAHGDWPVKPTPPF 61 Query: 65 LLGHEASGIVEEVGPGVRFVKPGDRVVLHWRKSQGIESLPPKYTWR---GKPLNAG--LI 119 + GHE +G V VG GV+ VK GDRV + W + P + W + N G + Sbjct: 62 IPGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTGYSVN 121 Query: 120 ATFNEYAIVSENRVTRIDPSSDMDVAALFGCA-VTTGFGVAENNADIRLGESVVVFGAGG 178 +F EY + V + + D AA CA VT G+ E ++R GE VV+ G GG Sbjct: 122 GSFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKET--EVRPGEWVVISGIGG 179 Query: 179 VGLNIIQAASLRSAYPIIAVDLHEGRLALAKQVGATHLINASQVDARTALLELTGKQGID 238 +G +Q A + ++A D+HE +LALAK++GA ++ +A + + G G Sbjct: 180 LGHMAVQYAKAMGMH-VVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIG--GAH 236 Query: 239 VFIDNTGQPSIIEMGYELTKAQGRVVLVGVPRAGRNISIYSLPLHFEKTISGSHGGEAVP 298 + P +E + +A+G + LVG+P ++ +++ L T+ GS G Sbjct: 237 GALVTAVSPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNTVLK-RITVRGSIVG---- 291 Query: 299 HVDIPRYTR--LLDAGLIQLKPLITEHF---KLDDINVAIQRMRSGAMSGRCLIRM 349 TR L ++ + + + HF KLD+IN RM G + GR ++ + Sbjct: 292 -------TRQDLEESLVFAAEGKVAAHFTWDKLDNINAIFARMEEGKIDGRIVLDL 340 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 100/369 (27%), Positives = 156/369 (42%), Gaps = 57/369 (15%) Query: 10 AILAELQQPLIIDEVELPPQLEVGQVLVKIGVSGVCGSQL----GEIDGAKGEDKYLPHL 65 A+L + ++ V P E V++K+ + +CGS L G+I K D + Sbjct: 3 ALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRGKIPATKAGD-----I 57 Query: 66 LGHEASGIVEEVGPGVRFVKPGDRVVLHWRKSQGIESLPPKYTW---------RGKPLNA 116 LGHE G+V E GP ++ +K GDRVV+ + + G ++ + RG +NA Sbjct: 58 LGHEFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGALMNA 117 Query: 117 GLI----ATFN-------------EYAIVSENRV--TRIDPSSDMDVAALFGCAVTTGFG 157 I A F EY V + V RID + + + TG+ Sbjct: 118 KGIRSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQ 177 Query: 158 VAENNADIRLGESVVVFGAGGVGLNIIQAASLRSAYPIIAVDLHEGRLALAKQVGATHLI 217 A NA +R G SV ++GAG VG +A + A I VD H RL A++ + Sbjct: 178 -AVKNAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPV 236 Query: 218 NASQVDARTALLELTGKQGIDVFIDNTG---QPSIIEMGYELTKAQG------RVVLVGV 268 N QVD ++ T +G+D ID G + S++E K +G R + V Sbjct: 237 NFDQVDPAEYIIANTAGRGVDGVIDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASV 296 Query: 269 PRAG--RNISIYSLPLH-------FEKTISGSHGGEAVPHVDIPRYTRLLDAGLIQLKPL 319 R G +Y+ +H FEK ++ G V H +P ++ G ++ + Sbjct: 297 RRGGVISVPGVYAGFIHGFLFGDIFEKGVTVKTGQTHVQHY-MPELLASIEEGRLRPDAI 355 Query: 320 ITEHFKLDD 328 IT L+D Sbjct: 356 ITHRLPLED 364 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 79.0 bits (193), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 89/347 (25%), Positives = 145/347 (41%), Gaps = 34/347 (9%) Query: 10 AILAELQQPLIIDEVELPPQLEVGQVLVKIGVSGVCGSQL-----GEIDGAKGEDKYLPH 64 A++ E + L + E++LP Q+ V ++I G+CGS L G I K E P Sbjct: 3 ALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVE---APM 59 Query: 65 LLGHEASGIVEEVGPGVRFVKPGDRVVLHW--------RKSQGIESLPPKYT-WRGKPLN 115 +LGHEASG V EVG V +K GDRV + +G+ +L P W P++ Sbjct: 60 VLGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWATPPIH 119 Query: 116 AGLIATFNEYAIVSENRVTRIDPSSDMDVAALFGCAVTTGFGVAENNADIRLGESVVVFG 175 L + A + + + V L + G A A ++ G++ VV G Sbjct: 120 GCLTGSVVHPAAFTYRLPDNVSFAEGAIVEPL-----SIGLQAA-TKARMKPGDTAVVIG 173 Query: 176 AGGVGLNIIQAASLRSAYPIIAVDLHEGRLALAKQVGATHLINASQVDARTALLELTGKQ 235 AG +G AA A +I D+ +LA A ++ ++ + + T Sbjct: 174 AGTIGAMTALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRETLTDVVRQATDGW 233 Query: 236 GIDVFIDNTGQPSIIEMGYELTKAQGRVVLVGVPRAGRNISIYSL---PLHFEKTISGSH 292 G DV + +G + + +L G VLVG+P A + + ++ + E ++ Sbjct: 234 GADVVFEASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYAN 293 Query: 293 GGEAVPHVDIPRYTRLLDAGLIQLKPLITEHFKLDDINVAIQRMRSG 339 PR L+ +G+I +KP I+ F A + SG Sbjct: 294 --------IFPRALALISSGMIDVKPFISRKFPFSQSIRAFEEAASG 332 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.139 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,157,501 Number of extensions: 53244 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 9 Length of query: 351 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 265 Effective length of database: 1,212,660 Effective search space: 321354900 Effective search space used: 321354900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)