BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0050.p (310 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309646|ref|YP_003773738.1| transketolase C-terminal subuni... 155 5e-39 gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subun... 144 6e-36 gi|300313125|ref|YP_003777217.1| 1-deoxy-D-xylulose 5-phosphate ... 111 7e-26 gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 59 5e-10 gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 58 9e-10 >gi|300309646|ref|YP_003773738.1| transketolase C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 321 Score = 155 bits (391), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 96/300 (32%), Positives = 152/300 (50%), Gaps = 12/300 (4%) Query: 1 MRNAFADEITKLGSSDPRVVLLSGDIGNKLFDKFKAANGTRFLNCGIAEANMMGVAAGMA 60 MR+A I + D ++ D G+ + DK +A RF+N GIAE N++ V+AG+A Sbjct: 8 MRDALLQRIWSAMAEDRQIFFTCADFGSPVLDKIRADFPDRFVNVGIAEQNLINVSAGLA 67 Query: 61 LSGLRPVIYTITPFTTTRCFEQIRVDACYHN----VPVIIVGTGSGLSYAELGPTHHSCE 116 L G + Y I PF T RC+EQ+RV + + V ++G G+G SY GPTH E Sbjct: 68 LEGYKVFAYAIAPFITMRCYEQVRVSLALLSEVRPMNVNLIGVGAGYSYVVSGPTHQCYE 127 Query: 117 DLAIMRVLPNMTVLAPADEVELRQCLRAALKLDGPVYIRIGKKGEQIVP--KQDEHFEIG 174 D+ +MR LPN VL+ AD V GP Y+R Q++P D ++ Sbjct: 128 DITLMRALPNFQVLSAADHVTAAAFFDTCSNSMGPKYLRFDA---QVLPVIYGDGAPDLA 184 Query: 175 RAINV-RSGSDVCLIGAGTLLPTVMAAADLLQARGISARVESFHTIKPLDEATLEQAFAG 233 + ++ R G + L+ G +L T + AA++L A GI V + L++ Sbjct: 185 QGFHIHRRGGKIGLVATGYMLHTALQAAEVLAAEGIEVGVIDLFDLARFSADALQETLQS 244 Query: 234 YAVVGVVEEHSR-IGGLGGAIAEWLARQEPMKGRLLGFGVDDSFMHEIGSQEYARAKYGL 292 YA + +EE R GG+ + E++AR+ ++ R+L GVD + E+G++ + G+ Sbjct: 245 YAGIATLEEGFRGRGGMDSMLFEFIARR-GLQTRMLNIGVDGVYRFELGTRTELHEEVGI 303 >gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 334 Score = 144 bits (364), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 96/301 (31%), Positives = 148/301 (49%), Gaps = 5/301 (1%) Query: 5 FADEITKLGSSDPRVVLLSGDIGNKL-FDKFKAANGTRFLNCGIAEANMMGVAAGMALSG 63 F + + P +V L+ D+ F+ A RF G+AE +M A GMA G Sbjct: 32 FGKALVDYAADRPEIVGLTADLAKYTDLHLFQKAYPERFFQMGMAEQLLMAAAGGMAKEG 91 Query: 64 LRPVIYTITPFTTTRCFEQIRVDACYHNVPVIIVGTGSGLSYAELGPTHHSCEDLAIMRV 123 L P T F T R ++ I N+ V I GL+ GP+H + EDLA+MR Sbjct: 92 LVPFATTYAVFATRRAYDFIHQVIAEENLNVKICCALPGLTTG-YGPSHQATEDLAMMRA 150 Query: 124 LPNMTVLAPADEVELRQCLRAALKLDGPVYIRIGKKGEQIVPKQ-DEHFEIGRAINVRSG 182 +P MT++ P D ++L Q + GPVY+R+ + +V + D FE+GRA +R G Sbjct: 151 IPGMTIVDPCDALDLEQAVPQIAAHQGPVYMRLARGNVPLVLDEYDYRFELGRAKEIRDG 210 Query: 183 SDVCLIGAGTLLPTVMAAADLLQARGISARVESFHTIKPLDEAT-LEQAFAGYAVVGVVE 241 +DV +I +G + + A L+A +S V TIKP DEAT + Q +V V E Sbjct: 211 NDVLIISSGFMTMRALEVAKALEADKVSVAVLHCPTIKPFDEATVVAQVKKKNRLVVVAE 270 Query: 242 EHSRIGGLGGAIAEWLARQEPMKGRLLGFGVDDSFMHEIGSQEYARAKYGLTADNIAAKV 301 HS +GGLG +A L R G+ D+F+ + G+ +YG++ + + ++ Sbjct: 271 NHSVVGGLGEIVATTLMRHRVQPAGFQLAGLPDAFL-DAGALPTLHDRYGISCEVLGRRI 329 Query: 302 E 302 + Sbjct: 330 K 330 >gi|300313125|ref|YP_003777217.1| 1-deoxy-D-xylulose 5-phosphate synthase [Herbaspirillum seropedicae SmR1] Length = 621 Score = 111 bits (278), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 94/308 (30%), Positives = 150/308 (48%), Gaps = 16/308 (5%) Query: 5 FADEITKLGSSDPRVVLLSGDI--GNKLFDKFKAANGTRFLNCGIAEANMMGVAAGMALS 62 F D + + ++D ++V ++ + G+ + D F+ R+ + GIAE + + AAGMA Sbjct: 315 FGDWLCDMAAADDKLVGITPAMREGSGMVD-FEKKFPNRYYDVGIAEQHAVTFAAGMACE 373 Query: 63 GLRPVIYTITPFTTTRCFEQIRVDACYHNVPVIIVGTGSGLSYAELGPTHHSCEDLAIMR 122 G++PV+ + F R ++Q+ D N+ V +GL A+ G TH DLA +R Sbjct: 374 GMKPVVAIYSTFLQ-RAYDQLIHDVALQNLDVTFALDRAGLVGAD-GATHAGNYDLAFLR 431 Query: 123 VLPNMTVLAPADEVELRQCLRAALKLDGPVYIRIGKKGEQIVPKQDEHFE---IGRAINV 179 +PNM V+A +DE E R+ L A + GP +R +G I D+ E +GR Sbjct: 432 CIPNMVVMAASDENECRKMLSTAYQYPGPASVRY-PRGAGIGAAVDQTLETLPLGRGEIR 490 Query: 180 RSGSDVCLIGAGTLLPTVMAAADLLQARGISARVESFHTIKPLDEATLEQAFAGYAVVGV 239 R G +V ++ G+LL + A D L +A V + IKPLD ++Q A + + Sbjct: 491 RQGKNVAILAFGSLLAPSLGAGDKL-----NATVANMRFIKPLDVELVKQLAASHDALVT 545 Query: 240 VEEHSRIGGLGGAIAEWLARQEPMKGRLLGFGVDDSFMHEIGSQEYARAKYGLTADNIAA 299 VEE +GG GA + +L G+ D F+ + G A+ GL A+ IA Sbjct: 546 VEEGCIMGG-AGAAVSEALAAAGINKPILHLGLPDRFI-DHGDAAQLLAQCGLDANGIAD 603 Query: 300 KVEAAFRK 307 + F K Sbjct: 604 SIAQRFGK 611 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 58.9 bits (141), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 73/314 (23%), Positives = 121/314 (38%), Gaps = 24/314 (7%) Query: 2 RNAFADEITKLGSSDPRVVLLSGDIGNKLFDKFKAANGTR------FLNCGIAEANMMGV 55 R A + I + P + S D+ +K + R +N G+ E M + Sbjct: 360 RKASQNAIQAYAQALPEFLGGSADLTGSNLTNWKESVAVRADQRGNHINYGVREFGMSAI 419 Query: 56 AAGMALSG-LRPVIYTITPFTTTRCFEQIRVDACYHNVPVIIVGTGSGLSYAELGPTHHS 114 G+AL G P T F+ +R+ A + + V T + E GPTH S Sbjct: 420 MNGIALHGGYLPFGATFLTFSD-YSRNALRM-AALMKIRALFVFTHDSIGLGEDGPTHQS 477 Query: 115 CEDLAIMRVLPNMTVLAPADEVELRQCLRAALKL-DGPVYIRIGKKGEQIVPKQDEHFEI 173 E ++ +R++PN+ P D E ++K DGP + ++ + D + Sbjct: 478 VEHVSSLRLIPNLDNWRPCDTTETAVAWAESIKRHDGPSTLIFSRQNLPFQERTDAQVKD 537 Query: 174 ----GRAINVRSGSDVCLIGAGTLLPTVMAAADLLQARGISARVESFHTIKPLDEATLEQ 229 G + + V L+ G+ + AAD L A GI+ RV S + D Sbjct: 538 IARGGYVLRDAPNAKVILMATGSEIELATKAADALTAEGIAVRVVSMPSTDVFDRQD--- 594 Query: 230 AFAGYAVVGVVEEHSRIGGLGGAIAEWLARQEPMKGRLLGFGVDDSFMHEIGSQEYARAK 289 A Y + R+ G A W + ++G ++G D+F E Sbjct: 595 --AAYKASVLTRGVPRVAIEAGVTAFW-HKYVGLEGAVVGI---DTF-GESAPAGVLFKH 647 Query: 290 YGLTADNIAAKVEA 303 +G T +N+ AK +A Sbjct: 648 FGFTVENVVAKAKA 661 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 58.2 bits (139), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 55/192 (28%), Positives = 86/192 (44%), Gaps = 12/192 (6%) Query: 42 FLNCGIAEANMMGVAAGMALSGLRPVIYTITPFTTTRCFEQ--IRVDACYHNVPVIIVGT 99 +++CG+ E M +A GMA G VI + +E+ +R+ A +PV V + Sbjct: 400 YVHCGVREHLMGAMANGMAAHG--GVIALSVTYLAFSDYERPAMRMSALM-GLPVKYVFS 456 Query: 100 GSGLSYAELGPTHHSCEDLAIMRVLPNMTVLAPADEVELRQCLRAALK-LDGPVYIRIGK 158 + + GPTH E LA +R +PNM V+ PAD E +C A++ GPV + + Sbjct: 457 HDSIGIGKNGPTHQPVEILASLRAMPNMLVMRPADATEAVECWEIAMQHRSGPVSMVFAR 516 Query: 159 KGEQIVPKQDE-HFEIGRAINVRS-----GSDVCLIGAGTLLPTVMAAADLLQARGISAR 212 + V +Q E R V V L+ G+ + AA L+A GI+ Sbjct: 517 QALPPVRRQYEAENRCARGGYVLHEAQCGARQVTLLATGSEVALACAARAQLEAAGIATA 576 Query: 213 VESFHTIKPLDE 224 V S + D+ Sbjct: 577 VVSLPCWELFDQ 588 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 966,591 Number of extensions: 42289 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 5 Length of query: 310 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 225 Effective length of database: 1,217,395 Effective search space: 273913875 Effective search space used: 273913875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)