BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0049.p (275 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [He... 169 2e-43 gi|300309645|ref|YP_003773737.1| transketolase N-terminal subuni... 164 1e-41 gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 101 7e-23 gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 90 2e-19 >gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [Herbaspirillum seropedicae SmR1] Length = 281 Score = 169 bits (428), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 97/257 (37%), Positives = 134/257 (52%), Gaps = 2/257 (0%) Query: 1 MAQDITALKKTASQLRGRIIENAHRTQTPHLGSCLSCVDILTAAYFGTLRIDPSRPADPL 60 M+ ++ L+ +A ++R + ++G L D+L AY + + P P Sbjct: 1 MSDNLANLRASAYRIRRNAVRMGEVQGQGYIGQALGWADVLAVAYKHAMSLRPDDPQWSG 60 Query: 61 RDRFILSKGHGAAALFQVLALRGFYPIAMLDDYGKDGGIFAEHPPTPDHLPGIEAATGSL 120 RDRF+LS GH A AL+ L G P LD YG D + PG+E + GSL Sbjct: 61 RDRFLLSHGHYAIALYAALIEAGIVPDEELDSYGSDDSRLP-MSGMATYTPGMEISGGSL 119 Query: 121 GHGLPIGLGMALAGRIQQQRYDVTVLLGDGECNEGSVWEAAMMAAAQRTGNLMVLVDFNK 180 G GL IG+GMAL R V + DGE +EGS WEAAM AA + GNL+ LVD N Sbjct: 120 GQGLAIGVGMALGLRYNNNPAFVYNSMSDGELDEGSTWEAAMGAAHHQLGNLICLVDINN 179 Query: 181 WQATGRSEEVLALNPLVDKWRAFGWDAMEIDGHDMATLVEIMGRPRA-PEGKPIAVVAHT 239 QA G S +++ PL DKW AFGW +DG+D+A +V+ R + KP ++ T Sbjct: 180 QQADGPSGKIMGFEPLADKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFDT 239 Query: 240 VKGKGVSFMEDDNNWHY 256 + GKGV F+E H+ Sbjct: 240 LMGKGVPFLEQREKNHF 256 >gi|300309645|ref|YP_003773737.1| transketolase N-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 272 Score = 164 bits (414), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 94/252 (37%), Positives = 139/252 (55%), Gaps = 4/252 (1%) Query: 1 MAQDITALKKTASQLRGRIIENAHRTQTPHLGSCLSCVDILTAAYFG-TLRIDPSRPADP 59 M D L+ A + + R L S LS +DI A Y+G LR +P+ P D Sbjct: 1 MKNDFVDLRAKAHWVWRETLAIHRRAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDA 60 Query: 60 LRDRFILSKGHGAAALFQVLALRGFYPIAMLDDYGKDGGIFAEHPPTPDHLPGIEAATGS 119 RDR ++SKGHG+ ++ +LA G++P+ L + + G F P P +PG E GS Sbjct: 61 GRDRCVISKGHGSICMYPILADLGYFPMEELQ-HVCEAGSFLGGIPDPV-IPGYETVNGS 118 Query: 120 LGHGLPIGLGMALAGRIQQQRYDVTVLLGDGECNEGSVWEAAMMAAAQRTGNLMVLVDFN 179 LGHGL + GMAL + V V+ GDGE +EG+ WEA M A+ + NL ++VD N Sbjct: 119 LGHGLGVATGMALGLKRSGSDRSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDN 178 Query: 180 KWQATGRSEEVLALNPLVDKWRAFGWDAMEIDGHD-MATLVEIMGRPRAPEGKPIAVVAH 238 + G +E++++ L + AFGWD ME+DGHD +A ++ + GKP A++A Sbjct: 179 RISMLGYTEDIVSHGELATRLSAFGWDCMEVDGHDVLAVQAALLQQKAHAAGKPKAIIAR 238 Query: 239 TVKGKGVSFMED 250 T+KG GV +E+ Sbjct: 239 TLKGHGVPGLEN 250 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 101 bits (251), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 81/273 (29%), Positives = 127/273 (46%), Gaps = 12/273 (4%) Query: 8 LKKTASQLRGRIIENAHRTQTPHLGSCLSCVDILTAAYFGTLRIDPSRPADPLRDRFILS 67 L+ A +R I+ R H G L +I TA Y LR +P+ P RDR +LS Sbjct: 6 LQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRDRVVLS 65 Query: 68 KGHGAAALFQVLALRGFYPIAM--LDDYGKDGGIFAEHPPTPDHLPGIEAATGSLGHGLP 125 GHG+ L+ +L L G+ I + + + + G A HP L GIE TG LG G+ Sbjct: 66 NGHGSMLLYCLLYLTGYEHITLEQVRSFREFGSHCAGHPEYAPEL-GIEVTTGPLGQGIA 124 Query: 126 IGLGMALAGRIQQQRY--DVT-----VLLGDGECNEGSVWEAAMMAAAQRTGNLMVLVDF 178 GMA+A R+ D+ +GDG EG E +A R L +L D Sbjct: 125 NAFGMAVAEAYLNARFGDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTLLWDD 184 Query: 179 NKWQATGRSEEVLALNPLVDKWRAFGWDAMEIDGHDMATLVEIMGRPRAPEGKPIAVVAH 238 N+ G++E ++ N + +++R GW +E+DGHD+ + + + + +P + Sbjct: 185 NQITDDGKTELSISEN-VAERFRVAGWHVVEVDGHDIQAVSRALDAAQGSD-RPSMIACR 242 Query: 239 TVKGKGVSFMEDDNNWHYRIPNADEVAAAYQEL 271 TV G+G+ ++ H D+ AA + L Sbjct: 243 TVIGRGIPRVQGQRGGHSARLYEDDAQAARELL 275 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 90.1 bits (222), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 76/277 (27%), Positives = 124/277 (44%), Gaps = 19/277 (6%) Query: 10 KTASQLRGRIIENAHRTQTPHLGSCLSCVDILTAAYFGTLRIDPSRPADPLRDRFILSKG 69 K A+ +R ++ + + H G + +I A + R +P+ P RDRF+LS G Sbjct: 9 KMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPANPHWANRDRFVLSNG 68 Query: 70 HGAAALFQVLALRGFYPIAMLD--DYGKDGGIFAEHPPTPDHL-PGIEAATGSLGHGLPI 126 HG+ + +L L G Y ++M D + + A HP H+ PG+E TG LG G+ Sbjct: 69 HGSMLQYALLHLTG-YDLSMEDIKSFRQLHSKTAGHPEV--HITPGVETTTGPLGQGITN 125 Query: 127 GLGMALAGRIQQQRYD----------VTVLLGDGECNEGSVWEAAMMAAAQRTGNLMVLV 176 +GMALA ++ ++ +GDG EG EA +A L+VL Sbjct: 126 AVGMALAEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSKLIVLY 185 Query: 177 DFNKWQATGRSEEVLALNPLVDKWRAFGWDAM-EIDGHDMATLVEIMGRPRAPEGKPIAV 235 D N G E + ++ A+GW+ + +DGH + + + + + + KP + Sbjct: 186 DDNGISIDGHVEGWFK-DDTPKRFEAYGWNVIPAVDGHSVEAVSAAIHQAKLAD-KPTLI 243 Query: 236 VAHTVKGKGVSFMEDDNNWHYRIPNADEVAAAYQELG 272 TV GKG + + H E+AA + LG Sbjct: 244 CCRTVIGKGSPNLAGTDKVHGAALGDKEIAAVREALG 280 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 991,867 Number of extensions: 46749 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 4 Length of query: 275 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 191 Effective length of database: 1,222,130 Effective search space: 233426830 Effective search space used: 233426830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)