BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0046.p (316 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309647|ref|YP_003773739.1| nucleoside-diphosphate-sugar ep... 129 2e-31 gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 65 7e-12 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 65 9e-12 gi|300309817|ref|YP_003773909.1| UDP-glucose 4-epimerase [Herbas... 59 6e-10 gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 57 2e-09 >gi|300309647|ref|YP_003773739.1| nucleoside-diphosphate-sugar epimerase [Herbaspirillum seropedicae SmR1] Length = 312 Score = 129 bits (325), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 99/303 (32%), Positives = 160/303 (52%), Gaps = 22/303 (7%) Query: 3 ILVTGGCGYKGSVLVPKLLARGYKVHVLDIMWFG----NYLAPHPNLTVTEGDVRNIDQI 58 ILVTGG GY GS+LVP+LL G++V V+D F N+ + N V GD+R+ D I Sbjct: 5 ILVTGGAGYIGSMLVPELLKLGHEVTVVDNFMFRQASLNHCCGYANFNVVNGDIRHEDFI 64 Query: 59 --NLDGVDTIIHLSSVANDPCGDLDPKLTWEVSALATMQLADKAVRKGVKQFIYASSGSV 116 + + + II L++ P + D V+ A M + + + ++ + ++ S Sbjct: 65 RPHYEAAEIIIPLAAYVGAPLCNKDKTGAQTVNHDAIMAML--GMLRDDQKVLMPTTNSA 122 Query: 117 YGVKEELQVT-EDLELKPISEYNKTKMVSEPVLLSYKDRMIVQIVRPATVCGYSPRMRLD 175 YG ++ E+ L PIS+Y K+ E L+ ++ + R ATV G +P+MR+D Sbjct: 123 YGSGDKDNFCDENSPLNPISKYAIDKVAIEKELMKRQNSISF---RLATVFGMAPKMRID 179 Query: 176 VSVNLLTMQALAKGKITVFGGTQVRPNIHIDDITDVYLHLIDHPEIT--GVYNAGFE--N 231 + VN +A+ I +F G R IH+ D+ +LH ID+ + ++N G N Sbjct: 180 LLVNDFVYRAVYDRFIVLFEGHFKRNYIHVTDVVRAFLHGIDNFDTMRGEIFNVGLSEAN 239 Query: 232 ISILDIARMVAKHIPVDIVVTE---SNDP--RSYRVNSDKLLATGFKPSKSVETAITEII 286 +S ++ + KHIP D+VV E + DP R+Y V++ KL ATG+KP ++ I E+I Sbjct: 240 VSKAELCAWIKKHIP-DLVVMEAPLATDPDQRNYVVSNAKLEATGWKPQVGLDAGIRELI 298 Query: 287 EKY 289 + Y Sbjct: 299 KGY 301 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 65.1 bits (157), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 75/308 (24%), Positives = 129/308 (41%), Gaps = 53/308 (17%) Query: 1 MNILVTGGCGYKGSVLVPKLLARGYKVHVLDIMWFGNYLAPHPN------------LTVT 48 MNILVTGG GY GS +LL G++V V D N+ P L++ Sbjct: 1 MNILVTGGAGYIGSHTCVELLKAGHEVIVFD-----NFCNSSPESVKRVQQITGKTLSLV 55 Query: 49 EGDVRNIDQINL----DGVDTIIHLSSVANDPCGDLDPKLTWEVSALATMQLADKAVRKG 104 +GD+RN +Q+ + +IH + + P + ++ + + T++L + G Sbjct: 56 KGDIRNREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATG 115 Query: 105 VKQFIYASSGSVYGVKEELQVTEDLELKPISEYNKTKMVSEPVLLSYKDR------MIVQ 158 VK +++SS +VYG + L + E L + Y ++K+V E +L Y +++ Sbjct: 116 VKTIVFSSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLR 175 Query: 159 IVRP-----ATVCGYSPRMRLDVSVNLLTMQALA----KGKITVFG-------GTQVRPN 202 P + + G P + NL+ A + ++ ++G GT VR Sbjct: 176 YFNPVGAHESGLIGEDPG---GIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDF 232 Query: 203 IHIDDITDVY---LHLIDHPEITGVYNAGFENISILDI----ARMVAKHIPVDIVVTESN 255 IH+ D+ + L +D P+ V S+LD+ + K +P I Sbjct: 233 IHVVDLARGHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPG 292 Query: 256 DPRSYRVN 263 D S N Sbjct: 293 DIASCYAN 300 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 64.7 bits (156), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 61/224 (27%), Positives = 103/224 (45%), Gaps = 24/224 (10%) Query: 3 ILVTGGCGYKGSVLVPKLLARGYKVHVLDIMWFGNYLA------------PHPNLTVTEG 50 I+V GG G GS V L+ K V +I+ + N+L P + G Sbjct: 8 IVVIGGAGLIGSHTVDHLV----KEDVKEILIYDNFLRGSRENLQNALKDPRVKIYDVGG 63 Query: 51 DVRNID--QINLDGVDTIIHLSSVANDPCGDLDPKLTWEVSALATMQLADKAVRKGVKQF 108 D+ D Q L+G D + HL+++ C + P+ ++V+ T + + V KGVK+ Sbjct: 64 DIMQTDILQSALEGADGVFHLAALWLLQCHEY-PRSAFDVNVRGTFNVMEACVAKGVKRL 122 Query: 109 IYASSGSVYGVKEELQVTEDLELKPISEYNKTKMVSEPVLLSYKDRMIVQIVRPATVCGY 168 +Y+SS SVYG + ED + Y TK+ E +L S+ R + V + Y Sbjct: 123 VYSSSASVYGDAVREPMDEDHPYNNKNFYGATKIAGETMLRSFHHRYGLNYVGLRYMNVY 182 Query: 169 SPRMRLD---VSVNLLTMQALAKGKI-TVFG-GTQVRPNIHIDD 207 PR ++V + + A+ KG+ T+ G G++ + ++D Sbjct: 183 GPRQDYHGAYIAVIMKMLDAIDKGESPTIMGDGSEAFDFVAVED 226 >gi|300309817|ref|YP_003773909.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 320 Score = 58.5 bits (140), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 69/246 (28%), Positives = 111/246 (45%), Gaps = 40/246 (16%) Query: 1 MNILVTGGCGYKGSVLVPKLLARGYKVHVLDIMWFGNYLAPHPNLTVTEGDVRNIDQINL 60 M+ILVTG G+ G L+ +L + G H+L G + P+ + T D I++ + Sbjct: 1 MHILVTGASGFVGRALLSRLDSEGQ--HLLR----GAF---RPS-SETVMDRERIERTVI 50 Query: 61 DGVD-------------TIIHLSS---VANDPCGDLDPKLTWEVSALATMQLADKAVRKG 104 G+D ++H ++ V N+ GD + ++ ++ LA +A + G Sbjct: 51 SGLDPETDWSAALHNIEAVVHCAARVHVMNETAGDPLAEFR-RINLEGSLALARQAQQAG 109 Query: 105 VKQFIYASSGSVYGVKEEL--QVTEDLELKPISEYNKTKMVSEPVLLSY--KDRMIVQIV 160 V++FI+ SS V G + L T D + P Y +KM +E LL +M V I+ Sbjct: 110 VQRFIFLSSIKVNGEQTSLGRPFTADDKPAPTDPYGISKMEAEQALLELGRTGKMQVVII 169 Query: 161 RPATVCGYSPRMRLDVSVNLLT-MQALAKGKITVFGGT--QVRPNIHIDDITDVYLHLID 217 RP V Y P V N L+ M+ L KG GG R + +D++ D+ + Sbjct: 170 RPVLV--YGP----GVKANFLSMMRWLDKGMPLPLGGITHNRRSLVALDNLVDLITVCLA 223 Query: 218 HPEITG 223 HP G Sbjct: 224 HPAAAG 229 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 45/161 (27%), Positives = 74/161 (45%), Gaps = 19/161 (11%) Query: 3 ILVTGGCGYKGSVLVPKLLARGYKVHVLD---------IMWFGNYLAPHPNLTVTEGDVR 53 ILVTGG GY GS +L+ GY V VLD I P+ +GD+R Sbjct: 6 ILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFV--QGDIR 63 Query: 54 NIDQINLDGV------DTIIHLSSVANDPCGDLDPKLTWEVSALATMQLADKAVRKGVKQ 107 D+ LDG+ + +IH + + P ++ + + L + GVK Sbjct: 64 --DRAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVKN 121 Query: 108 FIYASSGSVYGVKEELQVTEDLELKPISEYNKTKMVSEPVL 148 +++SS +VYG + +TE+ L + Y ++K++ E +L Sbjct: 122 IVFSSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQIL 162 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 943,766 Number of extensions: 37014 Number of successful extensions: 123 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 123 Number of HSP's successfully gapped: 7 Length of query: 316 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 231 Effective length of database: 1,217,395 Effective search space: 281218245 Effective search space used: 281218245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)